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The map label for this gene is aroE

Identifier: 78780183

GI number: 78780183

Start: 1694751

End: 1695611

Strand: Direct

Name: aroE

Synonym: PMT9312_1798

Alternate gene names: 78780183

Gene position: 1694751-1695611 (Clockwise)

Preceding gene: 78780181

Following gene: 78780184

Centisome position: 99.15

GC content: 30.43

Gene sequence:

>861_bases
ATGATTTCAAGTAAGACCTCTTTTATTGCATTAATCGGCAATCCAGTAAGCCATTCTTTGTCGCCAATCATGCAAAATGC
TGCCCTTCAGTATTTAGGCTTAGATTTAATTTATATTGCTATGCCTTGTAAAGATGAAGATCTAGAACTAGTTATGAATT
CTTTGAAAAAAATTAATTGCAAAGGTTTAAATATTACAATTCCCCATAAAGAAAAAGTATTTGACCTTTGTAGTGAAATT
TCTCCTATTGCTAATAAACTGAAAGCAATTAATACCTTAAAATTAAACTCTGCAAAAGAATGGAGCGCAACTAATACAGA
TGTTGAGGGATTTATTTATCCATTAAAAACATTAAATTTAACAAAGAAACAATCGATCGTTCTTGGCTCGGGTGGTGCAG
CAAGATCTGTTATTCAAGGATTAATAAATTTAAATTTTTCTACAATTTCAGTAGTATCACGTAACAAGTCATCACTAGAT
GAATTAATAAAAAATTTTGAAAATCAAATTACAATTGAGGGTTTGTTAAATAATGATAATAGGACTGAAACTTTAATTGA
AGAGGCAGATTTAATTGTAAATACAACACCTGTAGGAATGAAAACAACTAAACATGAGATGAATGTATTGCCATATGGGG
ACGTATTTTGGAGATCTCTTAACTCGAAAACAATTGTTTACGATTTAATCTACAATCCTGCTCCAACTCCTCTATTGAAA
TTTAGTGCCAAAAAAGGATGCATAACTATCGATGGTCTCAAAATGCTTGTTGCTCAAGGAGCGAAATCATTATCATTTTG
GACAAATGGTTTAGAAGTACCTTTTCATATTATGAATGACGCATTAAAAAATTATCTTTAA

Upstream 100 bases:

>100_bases
TGATATTCTCCATAATCGGTCATTGGCGTCATTATTGTTACTCAAAGACGGGGTGTGGTTCCCGAACAGATCGTTATTTT
TTAAGAAAAAATTTCTAAAC

Downstream 100 bases:

>100_bases
AAAAAATGATGCTAATTATTAAGAAACTGCACATCATAATGTATCGTAAATAATGAACAGACGCTCATCACATCAATCAT
GAGCCTAAGACCTTGTCTGA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 286

Protein sequence:

>286_residues
MISSKTSFIALIGNPVSHSLSPIMQNAALQYLGLDLIYIAMPCKDEDLELVMNSLKKINCKGLNITIPHKEKVFDLCSEI
SPIANKLKAINTLKLNSAKEWSATNTDVEGFIYPLKTLNLTKKQSIVLGSGGAARSVIQGLINLNFSTISVVSRNKSSLD
ELIKNFENQITIEGLLNNDNRTETLIEEADLIVNTTPVGMKTTKHEMNVLPYGDVFWRSLNSKTIVYDLIYNPAPTPLLK
FSAKKGCITIDGLKMLVAQGAKSLSFWTNGLEVPFHIMNDALKNYL

Sequences:

>Translated_286_residues
MISSKTSFIALIGNPVSHSLSPIMQNAALQYLGLDLIYIAMPCKDEDLELVMNSLKKINCKGLNITIPHKEKVFDLCSEI
SPIANKLKAINTLKLNSAKEWSATNTDVEGFIYPLKTLNLTKKQSIVLGSGGAARSVIQGLINLNFSTISVVSRNKSSLD
ELIKNFENQITIEGLLNNDNRTETLIEEADLIVNTTPVGMKTTKHEMNVLPYGDVFWRSLNSKTIVYDLIYNPAPTPLLK
FSAKKGCITIDGLKMLVAQGAKSLSFWTNGLEVPFHIMNDALKNYL
>Mature_286_residues
MISSKTSFIALIGNPVSHSLSPIMQNAALQYLGLDLIYIAMPCKDEDLELVMNSLKKINCKGLNITIPHKEKVFDLCSEI
SPIANKLKAINTLKLNSAKEWSATNTDVEGFIYPLKTLNLTKKQSIVLGSGGAARSVIQGLINLNFSTISVVSRNKSSLD
ELIKNFENQITIEGLLNNDNRTETLIEEADLIVNTTPVGMKTTKHEMNVLPYGDVFWRSLNSKTIVYDLIYNPAPTPLLK
FSAKKGCITIDGLKMLVAQGAKSLSFWTNGLEVPFHIMNDALKNYL

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1787983, Length=291, Percent_Identity=30.2405498281787, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI1789675, Length=261, Percent_Identity=28.735632183908, Blast_Score=104, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6320332, Length=279, Percent_Identity=25.8064516129032, Blast_Score=79, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_PROM9 (Q317T6)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_398295.1
- HSSP:   Q9SQT8
- ProteinModelPortal:   Q317T6
- STRING:   Q317T6
- GeneID:   3766625
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1798
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   NEDLEIV
- ProtClustDB:   PRK00258
- BioCyc:   PMAR74546:PMT9312_1798-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 31592; Mature: 31592

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: ACT_SITE 70-70

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISSKTSFIALIGNPVSHSLSPIMQNAALQYLGLDLIYIAMPCKDEDLELVMNSLKKINC
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCC
KGLNITIPHKEKVFDLCSEISPIANKLKAINTLKLNSAKEWSATNTDVEGFIYPLKTLNL
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEHHHCCC
TKKQSIVLGSGGAARSVIQGLINLNFSTISVVSRNKSSLDELIKNFENQITIEGLLNNDN
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCEEEEEEEECCCC
RTETLIEEADLIVNTTPVGMKTTKHEMNVLPYGDVFWRSLNSKTIVYDLIYNPAPTPLLK
HHHHHHHHCCEEEECCCCCCEECCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCEE
FSAKKGCITIDGLKMLVAQGAKSLSFWTNGLEVPFHIMNDALKNYL
ECCCCCCEEEHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MISSKTSFIALIGNPVSHSLSPIMQNAALQYLGLDLIYIAMPCKDEDLELVMNSLKKINC
CCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHCCC
KGLNITIPHKEKVFDLCSEISPIANKLKAINTLKLNSAKEWSATNTDVEGFIYPLKTLNL
CCEEEECCCHHHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEEEHHHCCC
TKKQSIVLGSGGAARSVIQGLINLNFSTISVVSRNKSSLDELIKNFENQITIEGLLNNDN
CCCCEEEEECCCHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHCCEEEEEEEECCCC
RTETLIEEADLIVNTTPVGMKTTKHEMNVLPYGDVFWRSLNSKTIVYDLIYNPAPTPLLK
HHHHHHHHCCEEEECCCCCCEECCCCCCCCCCCHHHHHHCCCCEEEEEEEECCCCCCCEE
FSAKKGCITIDGLKMLVAQGAKSLSFWTNGLEVPFHIMNDALKNYL
ECCCCCCEEEHHHHHHHHCCCCHHHHHCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA