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The map label for this gene is lig2 [H]

Identifier: 78780157

GI number: 78780157

Start: 1663219

End: 1664532

Strand: Direct

Name: lig2 [H]

Synonym: PMT9312_1772

Alternate gene names: 78780157

Gene position: 1663219-1664532 (Clockwise)

Preceding gene: 78780156

Following gene: 78780158

Centisome position: 97.31

GC content: 34.78

Gene sequence:

>1314_bases
TTGTTTAAAGAAGAAATAATACATCAATTAGAATTACACCCAAGTAGATTAGATAAAGAAAAAATCATCTCAGAAGCAAT
GGAAAATGGTCTAGATGATTTTTTTGAAGGCATTCGTATGGCACTTGATCCATTGGTAACTTTTGGTGTAAAAATTGTTC
CTGAGAAAGAGAATGAAAAAAGTCAAAGTTTTTTATGGAAAGATTTTAGAAAATTAGCAAATAAGCTTATTCAAAGAGAA
CTTACTGGTCACGCTGCGCGCGATGCAATTCTTGCGGCCATGGAATCTGCAACAAAAGAAGAGTGGAATGGATTTTATAG
ACGAGTTTTAATTAAAGATCTTAGATGTGGCGTATCTGAAAAAACAATCAACAAGATAGCAAAGAAATTTCCCGAATATT
CTATTCCTATTTTTTCTTGTCCTTTAGCTCATGACAGTGCAAATCATGAAAAAAAAATGATAGGGAAAAAGCAAATTGAA
ATCAAATTAGATGGTGTGCGCGTCTTAACTATTATTAGACAAAATAAAGTAGAGATGTTTTCTCGTAATGGGAAACAATT
CCATAATTTTGGTCATATTATCTCAGAAATAGAAAACGTCTTAAAAGAAGACCCAGCACCTTATGACTTAGTGCTAGATG
GTGAAGTAATGAGCGCTAACTTTCAGGATTTAATGAAACAGGTACATAGAAAAGATGGCAAACAATCCAAAGACGCAGTT
CTCCACCTATTTGACTTATGTCCCCTTGAAAACTTCCAAAAAGGAAGATGGAACACTAATCAAACAACAAGAAGTTTATT
AGTAAAAGAATGGGTAGCAAAACATTCTATGCTTCTAAAACATATACAAACACTTGAATGGGAAAATGTAGATCTCGATA
CCATTGAGGGACAGAAAAGATTTGTAGAGCTTAATAAATCTGCAGTGGAAGGAGGATATGAAGGAGTAATGATTAAAGAT
CCTGATGCTATGTATGAATGTAAAAGAACACACAGTTGGTTAAAAGCAAAACCTTTCATTGAAGTCACTTTAAAAATTGT
ATCGGTTGAGGAAGGCACAGGTCGAAATAAAGGTCGACTCGGAGCAGTCCTCGTAGAAGGGGAAGATGATGGTTATGAAT
ACAGTCTTAGTTGCGGCAGCGGATTTAGTGATATCCAACGTAAAGAATATTGGTCAAAACGTCATCAACTCATTGGTCAA
CTTGTAGAAATCAGAGCTGATGCTAAAACTAAATCCAAGGATGCGGTGGCTTTTAGTCTTAGGTTTCCTAGATTCAAGTG
CTTTAGAGGATTTAAAGCTGGAGAAAAAGTTTAA

Upstream 100 bases:

>100_bases
TGAGCTCCCATTAGATTTTACTTTCGCAAAAACTAACAATGATTGGAAAATAGACAGAATAGCTAAAGTGAATTAATAAT
TTCCTGTGAGGCAAAAAGAA

Downstream 100 bases:

>100_bases
ATTTTAAAAAATAATATTCCACAAACTAGTCAATTAAAAATGTAGTTTTTAAGTCAAAAAACTTGAAATCTTAACTATAA
AATATAAGAATAACTATCAG

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] 2 [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MFKEEIIHQLELHPSRLDKEKIISEAMENGLDDFFEGIRMALDPLVTFGVKIVPEKENEKSQSFLWKDFRKLANKLIQRE
LTGHAARDAILAAMESATKEEWNGFYRRVLIKDLRCGVSEKTINKIAKKFPEYSIPIFSCPLAHDSANHEKKMIGKKQIE
IKLDGVRVLTIIRQNKVEMFSRNGKQFHNFGHIISEIENVLKEDPAPYDLVLDGEVMSANFQDLMKQVHRKDGKQSKDAV
LHLFDLCPLENFQKGRWNTNQTTRSLLVKEWVAKHSMLLKHIQTLEWENVDLDTIEGQKRFVELNKSAVEGGYEGVMIKD
PDAMYECKRTHSWLKAKPFIEVTLKIVSVEEGTGRNKGRLGAVLVEGEDDGYEYSLSCGSGFSDIQRKEYWSKRHQLIGQ
LVEIRADAKTKSKDAVAFSLRFPRFKCFRGFKAGEKV

Sequences:

>Translated_437_residues
MFKEEIIHQLELHPSRLDKEKIISEAMENGLDDFFEGIRMALDPLVTFGVKIVPEKENEKSQSFLWKDFRKLANKLIQRE
LTGHAARDAILAAMESATKEEWNGFYRRVLIKDLRCGVSEKTINKIAKKFPEYSIPIFSCPLAHDSANHEKKMIGKKQIE
IKLDGVRVLTIIRQNKVEMFSRNGKQFHNFGHIISEIENVLKEDPAPYDLVLDGEVMSANFQDLMKQVHRKDGKQSKDAV
LHLFDLCPLENFQKGRWNTNQTTRSLLVKEWVAKHSMLLKHIQTLEWENVDLDTIEGQKRFVELNKSAVEGGYEGVMIKD
PDAMYECKRTHSWLKAKPFIEVTLKIVSVEEGTGRNKGRLGAVLVEGEDDGYEYSLSCGSGFSDIQRKEYWSKRHQLIGQ
LVEIRADAKTKSKDAVAFSLRFPRFKCFRGFKAGEKV
>Mature_437_residues
MFKEEIIHQLELHPSRLDKEKIISEAMENGLDDFFEGIRMALDPLVTFGVKIVPEKENEKSQSFLWKDFRKLANKLIQRE
LTGHAARDAILAAMESATKEEWNGFYRRVLIKDLRCGVSEKTINKIAKKFPEYSIPIFSCPLAHDSANHEKKMIGKKQIE
IKLDGVRVLTIIRQNKVEMFSRNGKQFHNFGHIISEIENVLKEDPAPYDLVLDGEVMSANFQDLMKQVHRKDGKQSKDAV
LHLFDLCPLENFQKGRWNTNQTTRSLLVKEWVAKHSMLLKHIQTLEWENVDLDTIEGQKRFVELNKSAVEGGYEGVMIKD
PDAMYECKRTHSWLKAKPFIEVTLKIVSVEEGTGRNKGRLGAVLVEGEDDGYEYSLSCGSGFSDIQRKEYWSKRHQLIGQ
LVEIRADAKTKSKDAVAFSLRFPRFKCFRGFKAGEKV

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 50295; Mature: 50295

Theoretical pI: Translated: 8.73; Mature: 8.73

Prosite motif: PS00697 DNA_LIGASE_A1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEEIIHQLELHPSRLDKEKIISEAMENGLDDFFEGIRMALDPLVTFGVKIVPEKENEK
CCHHHHHHHHHCCHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
SQSFLWKDFRKLANKLIQRELTGHAARDAILAAMESATKEEWNGFYRRVLIKDLRCGVSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KTINKIAKKFPEYSIPIFSCPLAHDSANHEKKMIGKKQIEIKLDGVRVLTIIRQNKVEMF
HHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHCCCEEEEEEECCEEEEEHHHHHHHHHH
SRNGKQFHNFGHIISEIENVLKEDPAPYDLVLDGEVMSANFQDLMKQVHRKDGKQSKDAV
HCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCHHHHH
LHLFDLCPLENFQKGRWNTNQTTRSLLVKEWVAKHSMLLKHIQTLEWENVDLDTIEGQKR
HHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
FVELNKSAVEGGYEGVMIKDPDAMYECKRTHSWLKAKPFIEVTLKIVSVEEGTGRNKGRL
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCE
GAVLVEGEDDGYEYSLSCGSGFSDIQRKEYWSKRHQLIGQLVEIRADAKTKSKDAVAFSL
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
RFPRFKCFRGFKAGEKV
ECCHHHHHCCCCCCCCC
>Mature Secondary Structure
MFKEEIIHQLELHPSRLDKEKIISEAMENGLDDFFEGIRMALDPLVTFGVKIVPEKENEK
CCHHHHHHHHHCCHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCEEEEECCCCCH
SQSFLWKDFRKLANKLIQRELTGHAARDAILAAMESATKEEWNGFYRRVLIKDLRCGVSE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
KTINKIAKKFPEYSIPIFSCPLAHDSANHEKKMIGKKQIEIKLDGVRVLTIIRQNKVEMF
HHHHHHHHHCCCCCCCEEECCCCCCCCCHHHHHCCCEEEEEEECCEEEEEHHHHHHHHHH
SRNGKQFHNFGHIISEIENVLKEDPAPYDLVLDGEVMSANFQDLMKQVHRKDGKQSKDAV
HCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCHHHHHHHHHHCCCCCHHHHH
LHLFDLCPLENFQKGRWNTNQTTRSLLVKEWVAKHSMLLKHIQTLEWENVDLDTIEGQKR
HHHHHHCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHH
FVELNKSAVEGGYEGVMIKDPDAMYECKRTHSWLKAKPFIEVTLKIVSVEEGTGRNKGRL
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHCCCCEEEEEEEEEEECCCCCCCCCE
GAVLVEGEDDGYEYSLSCGSGFSDIQRKEYWSKRHQLIGQLVEIRADAKTKSKDAVAFSL
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEE
RFPRFKCFRGFKAGEKV
ECCHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]