Click here to switch to the map view.

The map label for this gene is gcvH [H]

Identifier: 78780146

GI number: 78780146

Start: 1653599

End: 1653988

Strand: Reverse

Name: gcvH [H]

Synonym: PMT9312_1761

Alternate gene names: 78780146

Gene position: 1653988-1653599 (Counterclockwise)

Preceding gene: 78780147

Following gene: 78780145

Centisome position: 96.77

GC content: 35.13

Gene sequence:

>390_bases
ATGTCTTACCAGTTTCCAGACAACCTCAACTATGCTGATACCCATGAATATGTCTTAGAAGAAGAAGGATTGTTAAAAAT
TGGAGTTAGTGAATTTGCTATAGATCAATTAGGAGATATTGTTTTTGTTGAGTTAGCTGATCAAGGGGCTACTTTAGAGA
AAGGTGAGACTTTTGGAACAATAGAATCTGTTAAGGCCGTTGAGGAAGTCTATCTGCCTTTTTCAGGGGAAATAGTATCT
GTAAATGAAAGTGTTATTGATAACCCTGAGCTTTTACAGAATGATCCGATTGGAGAAGGTTGGTTAGTCATTTTGAAACC
AGAATCAAAAGTATCAATTGCTGATTTGATGACTTCTGAGGAATATAAATCAAAGGTTATGCCAAATTAA

Upstream 100 bases:

>100_bases
ACATAGCTCATATCAAAATTGCATTAATTCAATTATTATCTGAATTATTAGAGGAAAAATTAATTTCAAAGGATTCTCTA
CTTTCTTTATCAACTTAATC

Downstream 100 bases:

>100_bases
AGCAAAATCTTTATTAATAGGCTAAGTTATAGAAAAATTGGATATGAAATCCACCGTTAATTCAGATTTATTTATTAATA
GACACCTTGGTTTGAGTGAT

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 129; Mature: 128

Protein sequence:

>129_residues
MSYQFPDNLNYADTHEYVLEEEGLLKIGVSEFAIDQLGDIVFVELADQGATLEKGETFGTIESVKAVEEVYLPFSGEIVS
VNESVIDNPELLQNDPIGEGWLVILKPESKVSIADLMTSEEYKSKVMPN

Sequences:

>Translated_129_residues
MSYQFPDNLNYADTHEYVLEEEGLLKIGVSEFAIDQLGDIVFVELADQGATLEKGETFGTIESVKAVEEVYLPFSGEIVS
VNESVIDNPELLQNDPIGEGWLVILKPESKVSIADLMTSEEYKSKVMPN
>Mature_128_residues
SYQFPDNLNYADTHEYVLEEEGLLKIGVSEFAIDQLGDIVFVELADQGATLEKGETFGTIESVKAVEEVYLPFSGEIVSV
NESVIDNPELLQNDPIGEGWLVILKPESKVSIADLMTSEEYKSKVMPN

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI89057342, Length=113, Percent_Identity=37.1681415929204, Blast_Score=93, Evalue=7e-20,
Organism=Homo sapiens, GI49574537, Length=113, Percent_Identity=37.1681415929204, Blast_Score=92, Evalue=1e-19,
Organism=Escherichia coli, GI1789271, Length=120, Percent_Identity=37.5, Blast_Score=94, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=37.5, Blast_Score=96, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=39.2857142857143, Blast_Score=95, Evalue=8e-21,
Organism=Saccharomyces cerevisiae, GI6319272, Length=130, Percent_Identity=33.8461538461538, Blast_Score=91, Evalue=6e-20,
Organism=Drosophila melanogaster, GI17865652, Length=116, Percent_Identity=36.2068965517241, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14303; Mature: 14172

Theoretical pI: Translated: 3.72; Mature: 3.72

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQFPDNLNYADTHEYVLEEEGLLKIGVSEFAIDQLGDIVFVELADQGATLEKGETFGT
CCCCCCCCCCCCCCHHHHCCCCCEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
IESVKAVEEVYLPFSGEIVSVNESVIDNPELLQNDPIGEGWLVILKPESKVSIADLMTSE
HHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCCCCCCCEEEEECCCCCCHHHHHHHCH
EYKSKVMPN
HHHHCCCCC
>Mature Secondary Structure 
SYQFPDNLNYADTHEYVLEEEGLLKIGVSEFAIDQLGDIVFVELADQGATLEKGETFGT
CCCCCCCCCCCCCHHHHCCCCCEEEECHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCH
IESVKAVEEVYLPFSGEIVSVNESVIDNPELLQNDPIGEGWLVILKPESKVSIADLMTSE
HHHHHHHHHHCCCCCCCEEECCCCCCCCCHHHCCCCCCCCEEEEECCCCCCHHHHHHHCH
EYKSKVMPN
HHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA