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The map label for this gene is gyrB

Identifier: 78780112

GI number: 78780112

Start: 1612654

End: 1614621

Strand: Reverse

Name: gyrB

Synonym: PMT9312_1727

Alternate gene names: 78780112

Gene position: 1614621-1612654 (Counterclockwise)

Preceding gene: 78780117

Following gene: 78780111

Centisome position: 94.47

GC content: 34.25

Gene sequence:

>1968_bases
ATGAGTGAGGACAAAAGATCTAATAAAATCTCAAATGAATATGGTGCGGAACAGATTCAGGTCTTAGAGGGATTAGAGCC
AGTTCGTAAACGTCCTGGCATGTATATAGGTTCTACAGGTCCTAGAGGTTTGCATCATTTAGTATATGAAGTCGTTGATA
ATTCAGTTGATGAAGCACTTGCAGGTCATTGTGATCATATAGAAATAGTTCTTCAATCAGATGGATCAGCTTTAATTTCT
GATAATGGACGAGGTATCCCAACAGATATTCATCCAAGGACGGGTAAAAGTGCCTTAGAAACTGTACTGACTGTTCTTCA
TGCGGGAGGTAAATTCGGAAGTGGTGGCTATAAAGTTTCAGGCGGATTGCATGGAGTAGGAATCTCTGTAGTTAATGCTT
TAAGCGAATGGGTTAATGTGACTGTTTATAGAGATGGTAGTGAGTTTAATCAAAGATTTGAAAAAGGTGTATCGCAGAAT
GTATTGCAAAGTAAAAAGCAGACTAAAAAACCTTTTAAAAAAGGAACCACTATTTGTTTTAAACCTGATAAAACGATTTT
TTCTGGAGGAATCGAATTCGAATATGCTCTTCTCTCATCAAGATTAAGAGAACTTGCTTATCTTAATGGCGGTGTAAAAA
TTGTTTTTAGAGATGAGAGAAATAAATTGTCAGATGGTTCTTTTAAAGAAGAAATTTACTTATATCAAGGAGGCATTAAA
GAATATGTTCAATACATGAATGCGGAAAAAGAGGCCATTCATCCTGACATAATTTATGTTGACTCACAAAAAGAAAACGT
TTACGTAGAAGCAGCTTTGCAATGGTGTTCAGATGTATATTCAGATAATATTTTAGGTTTTGCAAATAATATTAGAACTA
TTGATGGAGGAACTCATATTGAAGGATTAAAGACAGTTTTGACAAGAACGTTCAATAATCTTGCAAAAAAAAGAGGTAAA
AGAAAAGATATTGAAAAAAATTTAGCTGGCGAAAATATCAGAGAGGGCTTAACTGTAGTTTTATCCGTTAAAGTTCCAGA
TCCCGAATTTGAGGGACAAACAAAAACAAAATTAGGTAATACGGAAGTAAGAGGAATTGTTGATTCTCTTATTGGGGAGG
CTCTTACAAAATTTATGGAATTTAATCCTGGAATTTTAGACTTGGTTCTTGAGAAAGCAATTCAATCATTCAATGCAGCA
GAAGCTGCAAGAAGGGCTAGAGAATTAGTAAGAAGAAAAAGTGTTCTTGAAAGCTCAACTTTGCCAGGTAAATTAGCAGA
TTGTAGTTCTAGAGATCCCGCAGAATCTGAAATTTATATCGTTGAGGGAGATTCTGCTGGAGGTTCCGCAAAACAAGGCA
GAGATAGAAAATTTCAGGCTATTTTGCCTCTTAGGGGTAAAATTCTAAATATCGAAAAAACTGACGATACAAAAATATAT
AAAAATACAGAAATTCAGTCTTTAATAACAGCTTTAGGATTAGGAATAAAAGGAGAGGAGTTCGACGTGAGCTCTTTGAG
ATATCACAGAGTTGTAATTATGACGGATGCTGATGTTGACGGTGCTCATATAAGAACCTTATTATTGACATTTTTTTATA
GATATCAAAGAGAACTTGTTGAAAAGGGATATATATATATTGCTTGTCCTCCCCTCTACAAGGTTGAAAGAGGTAAGAAT
CATAAATACTGTTATAACGAGAATCAATTAAAGGATACAATTGGAAGCTTTGGAGAAAATGCAAATTACAATATCCAAAG
ATTTAAGGGATTAGGTGAGATGATGCCAAAACAATTGTGGGATACAACTATGAATCCTCAAACCAGGATGATGAAAAGGG
TTGAAATTGAAGATGCACTTGAAGCTGACAGAATATTTAATATATTAATGGGAGATAAAGTTGCACCAAGAAGAGAATTT
ATTGAAACTCACAGTAGTAAATTAGATATGGCAACTTTAGATATATGA

Upstream 100 bases:

>100_bases
AGTGATTAAATTTTTCATAGACTTCGAGCCTTTCTCTTATTGCGGTGGTAAGTTTAATGAAAGGTCATTTTAAATGACCA
ATGTTTTTTTTTTATATTAA

Downstream 100 bases:

>100_bases
TTAATAATCTTCTTAAGAATTTTTGCTTAATTACTTTTGCATTAATTGCTCCAATTACATTACCTGCTGGTGGGATTCAA
AAAAGTATAAATCATAATAA

Product: DNA gyrase subunit B

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 655; Mature: 654

Protein sequence:

>655_residues
MSEDKRSNKISNEYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDHIEIVLQSDGSALIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSQN
VLQSKKQTKKPFKKGTTICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNKLSDGSFKEEIYLYQGGIK
EYVQYMNAEKEAIHPDIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGK
RKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKFMEFNPGILDLVLEKAIQSFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPAESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDVSSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGYIYIACPPLYKVERGKN
HKYCYNENQLKDTIGSFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREF
IETHSSKLDMATLDI

Sequences:

>Translated_655_residues
MSEDKRSNKISNEYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDHIEIVLQSDGSALIS
DNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSQN
VLQSKKQTKKPFKKGTTICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNKLSDGSFKEEIYLYQGGIK
EYVQYMNAEKEAIHPDIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGK
RKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKFMEFNPGILDLVLEKAIQSFNAA
EAARRARELVRRKSVLESSTLPGKLADCSSRDPAESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
KNTEIQSLITALGLGIKGEEFDVSSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGYIYIACPPLYKVERGKN
HKYCYNENQLKDTIGSFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREF
IETHSSKLDMATLDI
>Mature_654_residues
SEDKRSNKISNEYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEALAGHCDHIEIVLQSDGSALISD
NGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVSGGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSQNV
LQSKKQTKKPFKKGTTICFKPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNKLSDGSFKEEIYLYQGGIKE
YVQYMNAEKEAIHPDIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHIEGLKTVLTRTFNNLAKKRGKR
KDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGNTEVRGIVDSLIGEALTKFMEFNPGILDLVLEKAIQSFNAAE
AARRARELVRRKSVLESSTLPGKLADCSSRDPAESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIYK
NTEIQSLITALGLGIKGEEFDVSSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELVEKGYIYIACPPLYKVERGKNH
KYCYNENQLKDTIGSFGENANYNIQRFKGLGEMMPKQLWDTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFI
ETHSSKLDMATLDI

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=644, Percent_Identity=24.8447204968944, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI19913406, Length=615, Percent_Identity=23.739837398374, Blast_Score=127, Evalue=5e-29,
Organism=Escherichia coli, GI48994957, Length=610, Percent_Identity=50.4918032786885, Blast_Score=573, Evalue=1e-164,
Organism=Escherichia coli, GI1789408, Length=656, Percent_Identity=39.3292682926829, Blast_Score=435, Evalue=1e-123,
Organism=Caenorhabditis elegans, GI17535065, Length=575, Percent_Identity=23.1304347826087, Blast_Score=124, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645845, Length=547, Percent_Identity=25.2285191956124, Blast_Score=123, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=180, Percent_Identity=27.2222222222222, Blast_Score=79, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324241, Length=632, Percent_Identity=25.9493670886076, Blast_Score=132, Evalue=1e-31,
Organism=Drosophila melanogaster, GI17136538, Length=573, Percent_Identity=25.6544502617801, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR011557
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR006171 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c; PF01751 Toprim [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 73311; Mature: 73180

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEDKRSNKISNEYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEAL
CCCCCHHCCCHHHHCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDHIEIVLQSDGSALISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSQNVLQSKKQTKKPFKKGTTICF
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCHHHHHHHHHHHHHCHHHHCCEEEE
KPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNKLSDGSFKEEIYLYQGGIK
CCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCHH
EYVQYMNAEKEAIHPDIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCEECCCCCHH
EGLKTVLTRTFNNLAKKRGKRKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGEALTKFMEFNPGILDLVLEKAIQSFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPAESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDVSSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGYIYIACPPLYKVERGKNHKYCYNENQLKDTIGSFGENANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFIETHSSKLDMATLDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECC
>Mature Secondary Structure 
SEDKRSNKISNEYGAEQIQVLEGLEPVRKRPGMYIGSTGPRGLHHLVYEVVDNSVDEAL
CCCCHHCCCHHHHCHHHHHHHHCCHHHHHCCCEEEECCCCHHHHHHHHHHHHCHHHHHH
AGHCDHIEIVLQSDGSALISDNGRGIPTDIHPRTGKSALETVLTVLHAGGKFGSGGYKVS
HCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEC
GGLHGVGISVVNALSEWVNVTVYRDGSEFNQRFEKGVSQNVLQSKKQTKKPFKKGTTICF
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHCHHHHHHHHHHHHHCHHHHCCEEEE
KPDKTIFSGGIEFEYALLSSRLRELAYLNGGVKIVFRDERNKLSDGSFKEEIYLYQGGIK
CCCCCEECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEEECCHH
EYVQYMNAEKEAIHPDIIYVDSQKENVYVEAALQWCSDVYSDNILGFANNIRTIDGGTHI
HHHHHHCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCEECCCCCEECCCCCHH
EGLKTVLTRTFNNLAKKRGKRKDIEKNLAGENIREGLTVVLSVKVPDPEFEGQTKTKLGN
HHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHCCEEEEEEEECCCCCCCCCCHHCCCC
TEVRGIVDSLIGEALTKFMEFNPGILDLVLEKAIQSFNAAEAARRARELVRRKSVLESST
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCC
LPGKLADCSSRDPAESEIYIVEGDSAGGSAKQGRDRKFQAILPLRGKILNIEKTDDTKIY
CCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEEECCCCEEEEEECCCCCCEE
KNTEIQSLITALGLGIKGEEFDVSSLRYHRVVIMTDADVDGAHIRTLLLTFFYRYQRELV
CCCHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHH
EKGYIYIACPPLYKVERGKNHKYCYNENQLKDTIGSFGENANYNIQRFKGLGEMMPKQLW
HCCEEEEECCCCHHHCCCCCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHH
DTTMNPQTRMMKRVEIEDALEADRIFNILMGDKVAPRREFIETHSSKLDMATLDI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11466286 [H]