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The map label for this gene is btuR [C]

Identifier: 78780047

GI number: 78780047

Start: 1549609

End: 1550769

Strand: Reverse

Name: btuR [C]

Synonym: PMT9312_1662

Alternate gene names: 78780047

Gene position: 1550769-1549609 (Counterclockwise)

Preceding gene: 78780049

Following gene: 78780045

Centisome position: 90.73

GC content: 38.42

Gene sequence:

>1161_bases
TTGACGAATAACATTAGAAATCGAGGTATTGGAATTGTCACAGCAAGTGACAGTCAAGAGAGATTAAAAGGTCAATTACA
TATTTATGATGGCGAAGGTAAGGGTAAAAGTCAGGCTGCTTTAGGTGTTGTGCTGAGGACGATAGGTTTAGGAATATGCG
AAAAAAGACAGTCCAGAGTACTACTTCTTAGATTTTTAAAAGGTCCTGAGAGACCATATGATGAGGATTCTGCTATAGAG
GCCTTGCAGAGAGGATTCCCACACTTAATTGACCACGTAAGGACAGGAAGATCGGAATTCTTTGCTGCTGACCAGGTGAC
AAAGTTTGATATTGGTGAGGCTGAGAGAGGTTGGAATATTGCTAAAGGTGCAATTGCTAGTTCTCTCTATTCAGTAGTTG
TGCTTGATGAGCTGAATCCAGTTCTTGACTTAGGAATGCTTGATATCAAGGAAGTAGTTGATTCTCTTCAAAGTCGTCCT
GATGGATTGGAAATAATTATTACAGGAAGGGCAGCCCCTCCTTCTTTGGTAAGAATATCGCAACTCCATTCAGAAATGAG
ACCGAGACTAAGTGGAGACTTATCAAAAATAACAGGACAAAGGAGATTTAATGGTGGAATTGAGATTTATACAGGTGAGG
GAAAAGGGAAATCCACAAGTGCACTTGGCAAGGCTCTTCAAGCAATTGGTAAAGGAATATCTCAAGATAAAAGTCATAGA
GTTTTAATATTACAGTGGTTAAAAGGTGGAAATGGTTATACCGAAGATGCTGCCATAGATGCTTTGAGAGAAAGTTACCC
ACATTTGGTAGATCATTTGCGTTCAGGAAGAGATGCAATCGTTTGGAGAGGTCAGCAACAACCAATTGATTATGTTGAGG
CTGAAAGAGCATGGGAAATTGCTAAAGCTGCTATTTTGAGTGGTTTGTATAAAACAATTATTTTGGATGAATTGAATCCA
ACTGTTGATTTAGAGTTACTGCCTGTGGAATCAATACATCAAACGCTCTTAAAAAAACCAGCAGAAACAGAAGTTGTTAT
TACTGGGAGATGTAAAAATGAACCTTCATACTTTGAACTTGCTGATATTTACTCTGAAATGGTCTGTCATAAGCATTATG
CAAATGTTGGAGTTGATTTAAAAAGAGGGGTAGATTACTAA

Upstream 100 bases:

>100_bases
CTTTAAAATTCAAATTACCTATGTATAGGTTGAATTTATTATTAAATGTTTAATAATATTCTTCACTATATCCTAGATTA
AGTTTATTAACCTTGTTCCA

Downstream 100 bases:

>100_bases
ATATTTTTGAAATATTAATTGCATAATAATTTTTTTACCTTTTCAATGCCACCTTCAATAGATCTCGATTTAATTTTGAA
TTTAGACAAGATTTTGGAAG

Product: hypothetical protein

Products: Adenosylcobalamin; Phosphate; Diphosphate [C]

Alternate protein names: NA

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTNNIRNRGIGIVTASDSQERLKGQLHIYDGEGKGKSQAALGVVLRTIGLGICEKRQSRVLLLRFLKGPERPYDEDSAIE
ALQRGFPHLIDHVRTGRSEFFAADQVTKFDIGEAERGWNIAKGAIASSLYSVVVLDELNPVLDLGMLDIKEVVDSLQSRP
DGLEIIITGRAAPPSLVRISQLHSEMRPRLSGDLSKITGQRRFNGGIEIYTGEGKGKSTSALGKALQAIGKGISQDKSHR
VLILQWLKGGNGYTEDAAIDALRESYPHLVDHLRSGRDAIVWRGQQQPIDYVEAERAWEIAKAAILSGLYKTIILDELNP
TVDLELLPVESIHQTLLKKPAETEVVITGRCKNEPSYFELADIYSEMVCHKHYANVGVDLKRGVDY

Sequences:

>Translated_386_residues
MTNNIRNRGIGIVTASDSQERLKGQLHIYDGEGKGKSQAALGVVLRTIGLGICEKRQSRVLLLRFLKGPERPYDEDSAIE
ALQRGFPHLIDHVRTGRSEFFAADQVTKFDIGEAERGWNIAKGAIASSLYSVVVLDELNPVLDLGMLDIKEVVDSLQSRP
DGLEIIITGRAAPPSLVRISQLHSEMRPRLSGDLSKITGQRRFNGGIEIYTGEGKGKSTSALGKALQAIGKGISQDKSHR
VLILQWLKGGNGYTEDAAIDALRESYPHLVDHLRSGRDAIVWRGQQQPIDYVEAERAWEIAKAAILSGLYKTIILDELNP
TVDLELLPVESIHQTLLKKPAETEVVITGRCKNEPSYFELADIYSEMVCHKHYANVGVDLKRGVDY
>Mature_385_residues
TNNIRNRGIGIVTASDSQERLKGQLHIYDGEGKGKSQAALGVVLRTIGLGICEKRQSRVLLLRFLKGPERPYDEDSAIEA
LQRGFPHLIDHVRTGRSEFFAADQVTKFDIGEAERGWNIAKGAIASSLYSVVVLDELNPVLDLGMLDIKEVVDSLQSRPD
GLEIIITGRAAPPSLVRISQLHSEMRPRLSGDLSKITGQRRFNGGIEIYTGEGKGKSTSALGKALQAIGKGISQDKSHRV
LILQWLKGGNGYTEDAAIDALRESYPHLVDHLRSGRDAIVWRGQQQPIDYVEAERAWEIAKAAILSGLYKTIILDELNPT
VDLELLPVESIHQTLLKKPAETEVVITGRCKNEPSYFELADIYSEMVCHKHYANVGVDLKRGVDY

Specific function: Required For Both De Novo Synthesis Of The Corrin Ring For The Assimilation Of Exogenous Corrinoids. Participates In The Adenosylation Of A Variety Of Incomplete And Complete Corrinoids (By Similarity). [C]

COG id: COG2109

COG function: function code H; ATP:corrinoid adenosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787525, Length=210, Percent_Identity=29.5238095238095, Blast_Score=87, Evalue=3e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003724 [H]

Pfam domain/function: PF02572 CobA_CobO_BtuR [H]

EC number: 2.5.1.17 [C]

Molecular weight: Translated: 42656; Mature: 42525

Theoretical pI: Translated: 6.86; Mature: 6.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNNIRNRGIGIVTASDSQERLKGQLHIYDGEGKGKSQAALGVVLRTIGLGICEKRQSRV
CCCCCCCCCEEEEECCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LLLRFLKGPERPYDEDSAIEALQRGFPHLIDHVRTGRSEFFAADQVTKFDIGEAERGWNI
HHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHEECCCCEECCCCCCCCCCCH
AKGAIASSLYSVVVLDELNPVLDLGMLDIKEVVDSLQSRPDGLEIIITGRAAPPSLVRIS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
QLHSEMRPRLSGDLSKITGQRRFNGGIEIYTGEGKGKSTSALGKALQAIGKGISQDKSHR
HHHHHHCHHHCCCHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
VLILQWLKGGNGYTEDAAIDALRESYPHLVDHLRSGRDAIVWRGQQQPIDYVEAERAWEI
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
AKAAILSGLYKTIILDELNPTVDLELLPVESIHQTLLKKPAETEVVITGRCKNEPSYFEL
HHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
ADIYSEMVCHKHYANVGVDLKRGVDY
HHHHHHHHHHHHHHCCCCCHHCCCCC
>Mature Secondary Structure 
TNNIRNRGIGIVTASDSQERLKGQLHIYDGEGKGKSQAALGVVLRTIGLGICEKRQSRV
CCCCCCCCEEEEECCCCHHHCCCEEEEECCCCCCCHHHHHHHHHHHHCCHHHHHHHHHH
LLLRFLKGPERPYDEDSAIEALQRGFPHLIDHVRTGRSEFFAADQVTKFDIGEAERGWNI
HHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHCCHHHHEECCCCEECCCCCCCCCCCH
AKGAIASSLYSVVVLDELNPVLDLGMLDIKEVVDSLQSRPDGLEIIITGRAAPPSLVRIS
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHH
QLHSEMRPRLSGDLSKITGQRRFNGGIEIYTGEGKGKSTSALGKALQAIGKGISQDKSHR
HHHHHHCHHHCCCHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCCCC
VLILQWLKGGNGYTEDAAIDALRESYPHLVDHLRSGRDAIVWRGQQQPIDYVEAERAWEI
EEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHH
AKAAILSGLYKTIILDELNPTVDLELLPVESIHQTLLKKPAETEVVITGRCKNEPSYFEL
HHHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
ADIYSEMVCHKHYANVGVDLKRGVDY
HHHHHHHHHHHHHHCCCCCHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; Cob(I)alamin; H2O [C]

Specific reaction: ATP + Cob(I)alamin + H2O <==> Adenosylcobalamin + Phosphate + Diphosphate [C]

General reaction: Adenosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11759840; 7883709 [H]