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The map label for this gene is lipA1 [H]

Identifier: 78779990

GI number: 78779990

Start: 1506301

End: 1507194

Strand: Reverse

Name: lipA1 [H]

Synonym: PMT9312_1606

Alternate gene names: 78779990

Gene position: 1507194-1506301 (Counterclockwise)

Preceding gene: 78779993

Following gene: 78779987

Centisome position: 88.18

GC content: 34.34

Gene sequence:

>894_bases
TTGACTAAAAATCCTAATAGTTTAAGTTCAAAACCTGATTGGTTAAGAGTAAAAGCTCCGCAAGTTCAGAGAATTGGGGA
TACTGCAAATTTGTTAAATGATTTAAATCTCAATACTGTATGTCAAGAAGCAAGCTGTCCAAATATTGGTGAATGTTTTG
CTAGTGGAACTGCGACTTTTCTCATAATGGGTCCTGGCTGTACTAGGGCATGTCCATATTGTGATATTGATTTTGATAGA
TCTAAAAGGGAATTAGATCCAACAGAACCATATCGTCTAGCCGAAGCAGTTTATAGAATGAGGCTTAAACATGTTGTAAT
TACATCAGTTAATAGAGACGATCTTGAGGATGGTGGCGCATCTCAATTTTTTGAATGTGTTTATCAAGTAAGAAAAAAAT
CTCCTGAAACTACGATCGAGCTTTTAATTCCAGATTTTTGTGGCAACTGGAAAGCTCTTGAAAAAGTTCTTGATTCCAAT
CCAAACGTTTTAAACCACAATATAGAAACTGTGCCATCGCTATATAAAAAAGTAAGGCCTCAGGGTAAATATGAGAGAAC
TCTAGAGTTACTTAAAAGAACTAGAGATTATTCTCCTAAAGTTTATACAAAGTCTGGCTTTATGCTTGGTTTAGGGGAAA
AAGATGAGGAGGTTTTGAATCTTCTTGAGGATTTAAGGAGTAATTACGTTGATATAGTTACTATTGGTCAATATTTATCT
CCTGGCCCTAATCATTTACCTGTTCAAAGATTTGTGAGTCCTTCAAAATTTAATTATTTTAAAGTTTTTGGGGAAAAAGA
TTTGGACTTTATGCAAGTAGTTAGTTCTCCTCTAACTCGTAGCAGTTACCATGCAGAAGAGATTCAAAAACTTATGAAAA
AGTATCCAAGATAG

Upstream 100 bases:

>100_bases
ATTTTTTGACTTCTATTGAATAGGCTTCATCTTTGAAATTATTAATAGTTAATTTATCCTGTTTTTAGTAAAATAAAATT
ACAGGCAAGAACGAATTAAT

Downstream 100 bases:

>100_bases
TCATTTTTCATTGATCTGAATCTACCCACTCATTTAATTTCCACTCAACTAGATTATTTGTAATCATTGGATCATTTTTG
ATTATTTTTAGTGCATTTTT

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn 1; Lipoate synthase 1; Lipoic acid synthase 1; Sulfur insertion protein lipA1 [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTKNPNSLSSKPDWLRVKAPQVQRIGDTANLLNDLNLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDIDFDR
SKRELDPTEPYRLAEAVYRMRLKHVVITSVNRDDLEDGGASQFFECVYQVRKKSPETTIELLIPDFCGNWKALEKVLDSN
PNVLNHNIETVPSLYKKVRPQGKYERTLELLKRTRDYSPKVYTKSGFMLGLGEKDEEVLNLLEDLRSNYVDIVTIGQYLS
PGPNHLPVQRFVSPSKFNYFKVFGEKDLDFMQVVSSPLTRSSYHAEEIQKLMKKYPR

Sequences:

>Translated_297_residues
MTKNPNSLSSKPDWLRVKAPQVQRIGDTANLLNDLNLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDIDFDR
SKRELDPTEPYRLAEAVYRMRLKHVVITSVNRDDLEDGGASQFFECVYQVRKKSPETTIELLIPDFCGNWKALEKVLDSN
PNVLNHNIETVPSLYKKVRPQGKYERTLELLKRTRDYSPKVYTKSGFMLGLGEKDEEVLNLLEDLRSNYVDIVTIGQYLS
PGPNHLPVQRFVSPSKFNYFKVFGEKDLDFMQVVSSPLTRSSYHAEEIQKLMKKYPR
>Mature_296_residues
TKNPNSLSSKPDWLRVKAPQVQRIGDTANLLNDLNLNTVCQEASCPNIGECFASGTATFLIMGPGCTRACPYCDIDFDRS
KRELDPTEPYRLAEAVYRMRLKHVVITSVNRDDLEDGGASQFFECVYQVRKKSPETTIELLIPDFCGNWKALEKVLDSNP
NVLNHNIETVPSLYKKVRPQGKYERTLELLKRTRDYSPKVYTKSGFMLGLGEKDEEVLNLLEDLRSNYVDIVTIGQYLSP
GPNHLPVQRFVSPSKFNYFKVFGEKDLDFMQVVSSPLTRSSYHAEEIQKLMKKYPR

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives [H]

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family [H]

Homologues:

Organism=Homo sapiens, GI37577166, Length=283, Percent_Identity=43.1095406360424, Blast_Score=244, Evalue=1e-64,
Organism=Homo sapiens, GI37577164, Length=243, Percent_Identity=43.6213991769547, Blast_Score=213, Evalue=2e-55,
Organism=Escherichia coli, GI1786846, Length=289, Percent_Identity=45.3287197231834, Blast_Score=273, Evalue=1e-74,
Organism=Caenorhabditis elegans, GI32564533, Length=256, Percent_Identity=44.921875, Blast_Score=226, Evalue=1e-59,
Organism=Saccharomyces cerevisiae, GI6324770, Length=293, Percent_Identity=40.9556313993174, Blast_Score=228, Evalue=1e-60,
Organism=Drosophila melanogaster, GI221513272, Length=283, Percent_Identity=41.3427561837456, Blast_Score=227, Evalue=6e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: =2.8.1.8 [H]

Molecular weight: Translated: 33905; Mature: 33774

Theoretical pI: Translated: 7.97; Mature: 7.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKNPNSLSSKPDWLRVKAPQVQRIGDTANLLNDLNLNTVCQEASCPNIGECFASGTATF
CCCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEE
LIMGPGCTRACPYCDIDFDRSKRELDPTEPYRLAEAVYRMRLKHVVITSVNRDDLEDGGA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHCCCCCCH
SQFFECVYQVRKKSPETTIELLIPDFCGNWKALEKVLDSNPNVLNHNIETVPSLYKKVRP
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHCC
QGKYERTLELLKRTRDYSPKVYTKSGFMLGLGEKDEEVLNLLEDLRSNYVDIVTIGQYLS
CCHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCHHHHHHHHHHHHHCCEEEEEECHHHC
PGPNHLPVQRFVSPSKFNYFKVFGEKDLDFMQVVSSPLTRSSYHAEEIQKLMKKYPR
CCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
TKNPNSLSSKPDWLRVKAPQVQRIGDTANLLNDLNLNTVCQEASCPNIGECFASGTATF
CCCCCCCCCCCCEEEECCCHHHHHCHHHHHHHHCCHHHHHHCCCCCCHHHHHCCCCEEE
LIMGPGCTRACPYCDIDFDRSKRELDPTEPYRLAEAVYRMRLKHVVITSVNRDDLEDGGA
EEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCHHCCCCCCH
SQFFECVYQVRKKSPETTIELLIPDFCGNWKALEKVLDSNPNVLNHNIETVPSLYKKVRP
HHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCEECCCHHHHHHHHHHHCC
QGKYERTLELLKRTRDYSPKVYTKSGFMLGLGEKDEEVLNLLEDLRSNYVDIVTIGQYLS
CCHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCHHHHHHHHHHHHHCCEEEEEECHHHC
PGPNHLPVQRFVSPSKFNYFKVFGEKDLDFMQVVSSPLTRSSYHAEEIQKLMKKYPR
CCCCCCCHHHHCCCCCCCEEEEECCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]