Click here to switch to the map view.

The map label for this gene is nadE [H]

Identifier: 78779923

GI number: 78779923

Start: 1434924

End: 1436621

Strand: Direct

Name: nadE [H]

Synonym: PMT9312_1539

Alternate gene names: 78779923

Gene position: 1434924-1436621 (Clockwise)

Preceding gene: 78779922

Following gene: 78779935

Centisome position: 83.95

GC content: 30.98

Gene sequence:

>1698_bases
ATGAAGTTTTTACTTGCTCAAATTAATCCAGTAGTTGGGGATTTAGAAGGCAATGCAAAAAAAATACTTTATATTGCTTC
GCAAGCCTACTCAATTTCTGCTGATTTTGTTCTCACTCCAGAGTTGTCACTATGGGGCTATCCAGCTAATGACTTACTTC
TAAAGAAAAATTTAATCAACAATCAATCTCAAATACTTCACCAATTAGCGTTAGATATTAATAAAAAATATGGAAACTTA
AGCATCACAGTTGGAATAGCTGAGAGAATAAATGATTCTTTTTTCCCAAATCTTTATAATTCTATTGCTTTGATTGAGAA
CGGAGCATGGAAAATAATTGCTCGTAAAATTATTCTTCCCACTTATGAAGTATTTGATGAAAAAAGATACTTTAGATCAG
CAGAAAAAGTTTCGATATTGATAAAAAAAATTAAAAATAAAAATTGGCGAATTGGTTTTACTATATGTGAGGATTTATGG
GTCAACAAAGATATAGAAGGTAGAGGAATTCACAAAAAAAATCCTATTATTGATTTAAAGAAAAAAAAAGTTGATCTTTT
AGTGAATTTATCAGCCTCCCCATATACCTTTAAAAAGTTAGAGCTGAGATCTAAAGTTTCCAGTTTTGCTGCTCAATATC
TTCAAGTGCCCTTGATTTATGTGAACCAGATAGGCGCGAATGATAATTTAATTTTTGATGGGAATAGTTTTATTCTTGAT
AAGAATGGATCTAAAATTAAGCAATTAAAAGCTTTCTCAGAAGACCACTCCAGTTGGGAAATTGAGCAAACAAAACCTGA
AAAAAATAAATTTGAAACCTCTGAAATATCATCAATTTTTGATGCATTAGTTCTTGGTGTTAGGGATTATGCTAAAAAAT
GCGGATTTAAAACAGCTTTAATTGGATTAAGTGGTGGCATTGATTCGGCACTAGTTTCGGCAATTGCGACGGCTGCTCTT
GGCAGCAATAATATTTATTGTGTCTCAATGCCCTCTAAATGGAGCTCATCTCATTCAAAAAATGATGCGAAGGATTTAGC
GATAAGATTAAAAATAAATTTCAATAGTATCCCAATTGAAAATTTGATGACCTCTTTTGAAGAATCTTTAATAAAAACCT
TAACTTTCGAGATGGCTGAAATAACAAATCAAAATATTCAATCAAGGATCAGAGGAACGCTTCTGATGGCTTTAGCAAAT
CAAGAAAAGCATTTATTACTTTCAACAGGAAATAAATCGGAATTAGCTGTTGGATATTGCACACTTTATGGTGATATGAA
TGGAGGATTATCTGTAATTGGAGATTTATATAAAACTAATGTTTTTAAATTATGCAATTGGCTTGATGGTGAAGATTCAA
TTAATCATAGAAAATCATACATGTTAGACACTAATGTAAATATAATTGGAGAAAATATACGTACAAAAGCTCCAAGTGCC
GAATTAGGTCCTGATCAATTAGATACTGATTCTCTCCCACCTTATTCCACATTAGATAATATTCTTAAAGGAATTATTGA
AGAGAAAAAAGATTTAAAACAACTTGAAGAGGATGGTCATGAAAAAGAATTAATTTTAAAAATAATCTCACTTATAAAAA
AAGCGGAATTCAAAAGAAAGCAGGCTCCCCCTATCCTTAAACTAAGCAGTCAATCATTAGGAAGTGATTGGAGATTTCCA
ATTGCAATATCTTATTAA

Upstream 100 bases:

>100_bases
GTTCAAAATTCAGATTAAATTTTAATTATTCTAATTTACCAACATCCTTAATAGATATTGTTAAGAAGAATAATTTATAT
GAACCCACTAAATTAGTTGA

Downstream 100 bases:

>100_bases
TCGTTATAAGAACATTTTTGGATTTTTTTTAAAGGCCGTTTAATTGAATTAATATTGATACCTTTTTTTATAGCAAGAAT
TATACCTGCAGCTTCTAAAT

Product: NAD+ synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 565; Mature: 565

Protein sequence:

>565_residues
MKFLLAQINPVVGDLEGNAKKILYIASQAYSISADFVLTPELSLWGYPANDLLLKKNLINNQSQILHQLALDINKKYGNL
SITVGIAERINDSFFPNLYNSIALIENGAWKIIARKIILPTYEVFDEKRYFRSAEKVSILIKKIKNKNWRIGFTICEDLW
VNKDIEGRGIHKKNPIIDLKKKKVDLLVNLSASPYTFKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSKIKQLKAFSEDHSSWEIEQTKPEKNKFETSEISSIFDALVLGVRDYAKKCGFKTALIGLSGGIDSALVSAIATAAL
GSNNIYCVSMPSKWSSSHSKNDAKDLAIRLKINFNSIPIENLMTSFEESLIKTLTFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDGEDSINHRKSYMLDTNVNIIGENIRTKAPSA
ELGPDQLDTDSLPPYSTLDNILKGIIEEKKDLKQLEEDGHEKELILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRFP
IAISY

Sequences:

>Translated_565_residues
MKFLLAQINPVVGDLEGNAKKILYIASQAYSISADFVLTPELSLWGYPANDLLLKKNLINNQSQILHQLALDINKKYGNL
SITVGIAERINDSFFPNLYNSIALIENGAWKIIARKIILPTYEVFDEKRYFRSAEKVSILIKKIKNKNWRIGFTICEDLW
VNKDIEGRGIHKKNPIIDLKKKKVDLLVNLSASPYTFKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSKIKQLKAFSEDHSSWEIEQTKPEKNKFETSEISSIFDALVLGVRDYAKKCGFKTALIGLSGGIDSALVSAIATAAL
GSNNIYCVSMPSKWSSSHSKNDAKDLAIRLKINFNSIPIENLMTSFEESLIKTLTFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDGEDSINHRKSYMLDTNVNIIGENIRTKAPSA
ELGPDQLDTDSLPPYSTLDNILKGIIEEKKDLKQLEEDGHEKELILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRFP
IAISY
>Mature_565_residues
MKFLLAQINPVVGDLEGNAKKILYIASQAYSISADFVLTPELSLWGYPANDLLLKKNLINNQSQILHQLALDINKKYGNL
SITVGIAERINDSFFPNLYNSIALIENGAWKIIARKIILPTYEVFDEKRYFRSAEKVSILIKKIKNKNWRIGFTICEDLW
VNKDIEGRGIHKKNPIIDLKKKKVDLLVNLSASPYTFKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
KNGSKIKQLKAFSEDHSSWEIEQTKPEKNKFETSEISSIFDALVLGVRDYAKKCGFKTALIGLSGGIDSALVSAIATAAL
GSNNIYCVSMPSKWSSSHSKNDAKDLAIRLKINFNSIPIENLMTSFEESLIKTLTFEMAEITNQNIQSRIRGTLLMALAN
QEKHLLLSTGNKSELAVGYCTLYGDMNGGLSVIGDLYKTNVFKLCNWLDGEDSINHRKSYMLDTNVNIIGENIRTKAPSA
ELGPDQLDTDSLPPYSTLDNILKGIIEEKKDLKQLEEDGHEKELILKIISLIKKAEFKRKQAPPILKLSSQSLGSDWRFP
IAISY

Specific function: Can use both glutamine or ammonia as a nitrogen source [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 63371; Mature: 63371

Theoretical pI: Translated: 9.16; Mature: 9.16

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFLLAQINPVVGDLEGNAKKILYIASQAYSISADFVLTPELSLWGYPANDLLLKKNLIN
CCEEEECCCCCEECCCCCCEEEEEEEECCEEEEECEEECCCCEECCCCCCHHHHHHHHCC
NQSQILHQLALDINKKYGNLSITVGIAERINDSFFPNLYNSIALIENGAWKIIARKIILP
CHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCHHHHHCEEEEECCCCEEEEHHHHCC
TYEVFDEKRYFRSAEKVSILIKKIKNKNWRIGFTICEDLWVNKDIEGRGIHKKNPIIDLK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHCCCCCCCCCCCCCCCCEEEC
KKKVDLLVNLSASPYTFKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCEEEEE
KNGSKIKQLKAFSEDHSSWEIEQTKPEKNKFETSEISSIFDALVLGVRDYAKKCGFKTAL
CCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGLSGGIDSALVSAIATAALGSNNIYCVSMPSKWSSSHSKNDAKDLAIRLKINFNSIPIE
EEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCHH
NLMTSFEESLIKTLTFEMAEITNQNIQSRIRGTLLMALANQEKHLLLSTGNKSELAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDGEDSINHRKSYMLDTNVNIIGENIRTKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCC
ELGPDQLDTDSLPPYSTLDNILKGIIEEKKDLKQLEEDGHEKELILKIISLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRFPIAISY
CCCCEEEECCCCCCCCCCCCEEEEC
>Mature Secondary Structure
MKFLLAQINPVVGDLEGNAKKILYIASQAYSISADFVLTPELSLWGYPANDLLLKKNLIN
CCEEEECCCCCEECCCCCCEEEEEEEECCEEEEECEEECCCCEECCCCCCHHHHHHHHCC
NQSQILHQLALDINKKYGNLSITVGIAERINDSFFPNLYNSIALIENGAWKIIARKIILP
CHHHHHHHHHHHHCCCCCCEEEEEEEHHHCCCCCCHHHHHCEEEEECCCCEEEEHHHHCC
TYEVFDEKRYFRSAEKVSILIKKIKNKNWRIGFTICEDLWVNKDIEGRGIHKKNPIIDLK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCHHCCCCCCCCCCCCCCCCEEEC
KKKVDLLVNLSASPYTFKKLELRSKVSSFAAQYLQVPLIYVNQIGANDNLIFDGNSFILD
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCEEECCCEEEEE
KNGSKIKQLKAFSEDHSSWEIEQTKPEKNKFETSEISSIFDALVLGVRDYAKKCGFKTAL
CCCCHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
IGLSGGIDSALVSAIATAALGSNNIYCVSMPSKWSSSHSKNDAKDLAIRLKINFNSIPIE
EEECCCHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCEEEEEEEEECCCCCHH
NLMTSFEESLIKTLTFEMAEITNQNIQSRIRGTLLMALANQEKHLLLSTGNKSELAVGYC
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEECCCCCCEEEEEE
TLYGDMNGGLSVIGDLYKTNVFKLCNWLDGEDSINHRKSYMLDTNVNIIGENIRTKAPSA
EEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCC
ELGPDQLDTDSLPPYSTLDNILKGIIEEKKDLKQLEEDGHEKELILKIISLIKKAEFKRK
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHH
QAPPILKLSSQSLGSDWRFPIAISY
CCCCEEEECCCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]