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The map label for this gene is cobD [H]

Identifier: 78779797

GI number: 78779797

Start: 1329266

End: 1330285

Strand: Direct

Name: cobD [H]

Synonym: PMT9312_1413

Alternate gene names: 78779797

Gene position: 1329266-1330285 (Clockwise)

Preceding gene: 78779796

Following gene: 78779798

Centisome position: 77.77

GC content: 27.84

Gene sequence:

>1020_bases
TTGGCTGAAATAAATTTATTTTTTATTTTTCTTGGATCTATTGGTTTTGATTTATTAATCGGCGACCCAAGATTTCTTAT
CCACCCTGTTCAAATCATTGGCTTCTACATAAAAAAAATATCTGATTACCTCATAAATAATTTTGGAGAAAATAAAAAGA
TATTGTTATGGGGTGGTTTCATTGTCGCTATATCTACTATTGGTTTGAGTTTCAGTTTAGGGAAATTAATAGAACTAAGT
TATGTGCAATCAAGAAATAATTTTTTTAGTGGGCTTTTAATTTTTTTGGGACTTTCAAGTTGTATTGCTACTAATGGACT
TATTTCAAGTGTAAAAGAGATTGCAGAGCTAATAGAAGGCAAGGAAATTAATGATCAAAATAATAGACTAATCAAAGTGA
AGGTTCAAAGAATAGTAAGTAGGGATGTAAGTTCATCTTCTATAGAACATCTTTTGAGATCAAGTACCGAAAGTCTTACC
GAAAATTCTGTTGATGGAATATTTGGACCATTATTTTGGATTTTTATTGGAATTATTTTTATGAAGTTTTCATTTTTTCT
ACCAGGGCCTTTGTCACTTGGTTTTTCTTATAAAGCAATAAGTACTTTAGATTCAATGTTAGGTTACAAATATGATTACT
TTAGATATTTAGGTTTTTTCAGTGCAAAAATCGAAGATATCTTTACGTTTGTTCCTTCAAGATTAGTTTTAATCACATTA
CCTTTAGTTGGTTCCAAAGTTAATGAGTATGTACCAATAATAATAAAAAGTTATCTTGATGGTAAAAAATATGATTCGCC
TAATGCTGGGATTTCAGAAGCTATATTTGCTTATATTTCGGGTATTAAATTGGGAGGAAAAAGTAAATATAAAAATGAAA
TTATTGAAAAGCCAATAATCAATGCGACTGGAGATAATTGTACTGGAGAGAAAATTAAATTAATTTGTCAACTAATTTTG
AGATTACAATTTTTATGGATAATAATTTTTATCTTAATTTTTTTATGCTTTCGAGTTTAA

Upstream 100 bases:

>100_bases
AAGAATGCGATAAAAATAAACCCTTAATGACAAAATTAAGAGAAGAGAACTCAAAACATGAAATTGAGAAAGTCGGTAAA
GGTCTTCGCTCTATGTTCAG

Downstream 100 bases:

>100_bases
TTTAATAATAAATTAGTTTAAAAATTATTAATATAAATAAAAAAGACTCTATGCAAGAAAAAGACTCTCCAATGGAAAAT
CAAAATGATGATTTTACTAA

Product: adenosylcobinamide-phosphate synthase

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MAEINLFFIFLGSIGFDLLIGDPRFLIHPVQIIGFYIKKISDYLINNFGENKKILLWGGFIVAISTIGLSFSLGKLIELS
YVQSRNNFFSGLLIFLGLSSCIATNGLISSVKEIAELIEGKEINDQNNRLIKVKVQRIVSRDVSSSSIEHLLRSSTESLT
ENSVDGIFGPLFWIFIGIIFMKFSFFLPGPLSLGFSYKAISTLDSMLGYKYDYFRYLGFFSAKIEDIFTFVPSRLVLITL
PLVGSKVNEYVPIIIKSYLDGKKYDSPNAGISEAIFAYISGIKLGGKSKYKNEIIEKPIINATGDNCTGEKIKLICQLIL
RLQFLWIIIFILIFLCFRV

Sequences:

>Translated_339_residues
MAEINLFFIFLGSIGFDLLIGDPRFLIHPVQIIGFYIKKISDYLINNFGENKKILLWGGFIVAISTIGLSFSLGKLIELS
YVQSRNNFFSGLLIFLGLSSCIATNGLISSVKEIAELIEGKEINDQNNRLIKVKVQRIVSRDVSSSSIEHLLRSSTESLT
ENSVDGIFGPLFWIFIGIIFMKFSFFLPGPLSLGFSYKAISTLDSMLGYKYDYFRYLGFFSAKIEDIFTFVPSRLVLITL
PLVGSKVNEYVPIIIKSYLDGKKYDSPNAGISEAIFAYISGIKLGGKSKYKNEIIEKPIINATGDNCTGEKIKLICQLIL
RLQFLWIIIFILIFLCFRV
>Mature_338_residues
AEINLFFIFLGSIGFDLLIGDPRFLIHPVQIIGFYIKKISDYLINNFGENKKILLWGGFIVAISTIGLSFSLGKLIELSY
VQSRNNFFSGLLIFLGLSSCIATNGLISSVKEIAELIEGKEINDQNNRLIKVKVQRIVSRDVSSSSIEHLLRSSTESLTE
NSVDGIFGPLFWIFIGIIFMKFSFFLPGPLSLGFSYKAISTLDSMLGYKYDYFRYLGFFSAKIEDIFTFVPSRLVLITLP
LVGSKVNEYVPIIIKSYLDGKKYDSPNAGISEAIFAYISGIKLGGKSKYKNEIIEKPIINATGDNCTGEKIKLICQLILR
LQFLWIIIFILIFLCFRV

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 38148; Mature: 38016

Theoretical pI: Translated: 9.17; Mature: 9.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEINLFFIFLGSIGFDLLIGDPRFLIHPVQIIGFYIKKISDYLINNFGENKKILLWGGF
CCCEEEEEEEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHH
IVAISTIGLSFSLGKLIELSYVQSRNNFFSGLLIFLGLSSCIATNGLISSVKEIAELIEG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KEINDQNNRLIKVKVQRIVSRDVSSSSIEHLLRSSTESLTENSVDGIFGPLFWIFIGIIF
CCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MKFSFFLPGPLSLGFSYKAISTLDSMLGYKYDYFRYLGFFSAKIEDIFTFVPSRLVLITL
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
PLVGSKVNEYVPIIIKSYLDGKKYDSPNAGISEAIFAYISGIKLGGKSKYKNEIIEKPII
CHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
NATGDNCTGEKIKLICQLILRLQFLWIIIFILIFLCFRV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AEINLFFIFLGSIGFDLLIGDPRFLIHPVQIIGFYIKKISDYLINNFGENKKILLWGGF
CCEEEEEEEEHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHH
IVAISTIGLSFSLGKLIELSYVQSRNNFFSGLLIFLGLSSCIATNGLISSVKEIAELIEG
HHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
KEINDQNNRLIKVKVQRIVSRDVSSSSIEHLLRSSTESLTENSVDGIFGPLFWIFIGIIF
CCCCCCCCCEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
MKFSFFLPGPLSLGFSYKAISTLDSMLGYKYDYFRYLGFFSAKIEDIFTFVPSRLVLITL
HHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEE
PLVGSKVNEYVPIIIKSYLDGKKYDSPNAGISEAIFAYISGIKLGGKSKYKNEIIEKPII
CHHCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCC
NATGDNCTGEKIKLICQLILRLQFLWIIIFILIFLCFRV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]