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The map label for this gene is ddl

Identifier: 78779786

GI number: 78779786

Start: 1319174

End: 1320241

Strand: Direct

Name: ddl

Synonym: PMT9312_1402

Alternate gene names: 78779786

Gene position: 1319174-1320241 (Clockwise)

Preceding gene: 78779785

Following gene: 78779787

Centisome position: 77.18

GC content: 28.0

Gene sequence:

>1068_bases
ATGATCGAGGAAAAGAAAAAATGTATTGGATTAATATTTGGTGGATATTCCAATGAGCATGATGTATCAATATCCTCTGC
TAAGACAGTTTTTAATGCCTTTAATTCAGAAATTAATAAACAACGCTTTAATGTTAAAGCCTTTTACATAAATAAATATG
GAGATTGGATTGATAATGATATCTCAGTTAAGATCCTGATTGGTGAAATTGAACACAAAAAAACAAAAAAACAAGAGCTT
TTTAATCAAAAAAAAATTAACTTCCTTGACGGAATTGAATTTCAAAATATTGATATTTGGTTTCCACTTCTTCATGGATT
TAATGGCGAAGATGGATCAATTCATGGATTACTAAAATTCACAAATAAACCTTTAGTCGGATGTGGGATTCTTGGCTCTG
CTCTTGGTATGGATAAAATATTGATGAAGACAATTTTCTCAAATCTAAAAATTCCACAAGTTAATTATTTAGTTATTCAA
AACGAAGATTTGAATAATCTAGAAGTAAAAAATAAGTTAATTATTGAGATAATAAAAAAATTAAATTTTCCTGTTTTTGT
TAAACCATCTAACTCTGGTTCATCCCTTGGCATTTCTAAAGTAATAAATAAATCAGCATTATTAAAAGCTTTAGAAAAGG
CTTGGGAAATAGATGCAAGAATTTTAGTAGAAGAAGGTTTAGAGACAAGAGAGATTGAATGCGGAATAATTGGGAATTCA
GAACTCTTAACCTCTGAGATTGGAGAGGTAGAATACGGAAGTGATTGGTATGATTATGATTCAAAATATAACTCAAATAA
TAAGATAACTATCCCAGCCAAAATAGATTCTAAAATCACTAAACAAATTAAAGAAATTGCTATTCAAAGTTGTAGAACAC
TAAATATTTTTGGTTTTGCAAGGGTAGATTTCTTTTTAGAAAAATCTTCAAATAAAATTTTTTTAAATGAAATAAATACA
ATTCCTGGTTTTACAAAAAAAAGTATGTTTCCAATGCTCTGGGAAGCTTCGGGTTTAAATATTGAACAACTTGTGGCTAA
ACTAGTAGATATATCTCTAGATTTATAA

Upstream 100 bases:

>100_bases
ATTCAATTTAATTTTTCACTTGGAGATGAGATAGATGTCAGAATTAATGAAGCAAGACCTTTTTCTTTAACAGGTGAACT
TTGCTAAAAATTTTTTCTAA

Downstream 100 bases:

>100_bases
TTCAAATCAAATGTTGCATGGACTTCTTTGGTTACCATTACTTTTAATCTTCGTTTTATTAACTGCACTTGGATGGTTAG
AGAGAAGAAGGCAAAATCTT

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase

Number of amino acids: Translated: 355; Mature: 355

Protein sequence:

>355_residues
MIEEKKKCIGLIFGGYSNEHDVSISSAKTVFNAFNSEINKQRFNVKAFYINKYGDWIDNDISVKILIGEIEHKKTKKQEL
FNQKKINFLDGIEFQNIDIWFPLLHGFNGEDGSIHGLLKFTNKPLVGCGILGSALGMDKILMKTIFSNLKIPQVNYLVIQ
NEDLNNLEVKNKLIIEIIKKLNFPVFVKPSNSGSSLGISKVINKSALLKALEKAWEIDARILVEEGLETREIECGIIGNS
ELLTSEIGEVEYGSDWYDYDSKYNSNNKITIPAKIDSKITKQIKEIAIQSCRTLNIFGFARVDFFLEKSSNKIFLNEINT
IPGFTKKSMFPMLWEASGLNIEQLVAKLVDISLDL

Sequences:

>Translated_355_residues
MIEEKKKCIGLIFGGYSNEHDVSISSAKTVFNAFNSEINKQRFNVKAFYINKYGDWIDNDISVKILIGEIEHKKTKKQEL
FNQKKINFLDGIEFQNIDIWFPLLHGFNGEDGSIHGLLKFTNKPLVGCGILGSALGMDKILMKTIFSNLKIPQVNYLVIQ
NEDLNNLEVKNKLIIEIIKKLNFPVFVKPSNSGSSLGISKVINKSALLKALEKAWEIDARILVEEGLETREIECGIIGNS
ELLTSEIGEVEYGSDWYDYDSKYNSNNKITIPAKIDSKITKQIKEIAIQSCRTLNIFGFARVDFFLEKSSNKIFLNEINT
IPGFTKKSMFPMLWEASGLNIEQLVAKLVDISLDL
>Mature_355_residues
MIEEKKKCIGLIFGGYSNEHDVSISSAKTVFNAFNSEINKQRFNVKAFYINKYGDWIDNDISVKILIGEIEHKKTKKQEL
FNQKKINFLDGIEFQNIDIWFPLLHGFNGEDGSIHGLLKFTNKPLVGCGILGSALGMDKILMKTIFSNLKIPQVNYLVIQ
NEDLNNLEVKNKLIIEIIKKLNFPVFVKPSNSGSSLGISKVINKSALLKALEKAWEIDARILVEEGLETREIECGIIGNS
ELLTSEIGEVEYGSDWYDYDSKYNSNNKITIPAKIDSKITKQIKEIAIQSCRTLNIFGFARVDFFLEKSSNKIFLNEINT
IPGFTKKSMFPMLWEASGLNIEQLVAKLVDISLDL

Specific function: Cell wall formation

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1786579, Length=357, Percent_Identity=36.9747899159664, Blast_Score=251, Evalue=4e-68,
Organism=Escherichia coli, GI1786280, Length=345, Percent_Identity=28.1159420289855, Blast_Score=147, Evalue=1e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DDL_PROM9 (Q319I3)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397898.1
- ProteinModelPortal:   Q319I3
- SMR:   Q319I3
- STRING:   Q319I3
- GeneID:   3766216
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1402
- eggNOG:   COG1181
- HOGENOM:   HBG427092
- OMA:   FTDISMY
- ProtClustDB:   PRK01966
- BioCyc:   PMAR74546:PMT9312_1402-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00047
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR01205

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N; SSF52440 PreATP-grasp-like

EC number: =6.3.2.4

Molecular weight: Translated: 40176; Mature: 40176

Theoretical pI: Translated: 6.94; Mature: 6.94

Prosite motif: PS50975 ATP_GRASP; PS00843 DALA_DALA_LIGASE_1; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEEKKKCIGLIFGGYSNEHDVSISSAKTVFNAFNSEINKQRFNVKAFYINKYGDWIDND
CCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCC
ISVKILIGEIEHKKTKKQELFNQKKINFLDGIEFQNIDIWFPLLHGFNGEDGSIHGLLKF
CEEEEEEECCHHHHHHHHHHHHHCCCCHHCCCEECCEEEEEEHHCCCCCCCCCEEEEEEE
TNKPLVGCGILGSALGMDKILMKTIFSNLKIPQVNYLVIQNEDLNNLEVKNKLIIEIIKK
CCCCEEEHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
LNFPVFVKPSNSGSSLGISKVINKSALLKALEKAWEIDARILVEEGLETREIECGIIGNS
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCC
ELLTSEIGEVEYGSDWYDYDSKYNSNNKITIPAKIDSKITKQIKEIAIQSCRTLNIFGFA
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEEE
RVDFFLEKSSNKIFLNEINTIPGFTKKSMFPMLWEASGLNIEQLVAKLVDISLDL
EEEEEEECCCCEEEEEECCCCCCCCHHCCCCCEEECCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIEEKKKCIGLIFGGYSNEHDVSISSAKTVFNAFNSEINKQRFNVKAFYINKYGDWIDND
CCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCCCCCCC
ISVKILIGEIEHKKTKKQELFNQKKINFLDGIEFQNIDIWFPLLHGFNGEDGSIHGLLKF
CEEEEEEECCHHHHHHHHHHHHHCCCCHHCCCEECCEEEEEEHHCCCCCCCCCEEEEEEE
TNKPLVGCGILGSALGMDKILMKTIFSNLKIPQVNYLVIQNEDLNNLEVKNKLIIEIIKK
CCCCEEEHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHH
LNFPVFVKPSNSGSSLGISKVINKSALLKALEKAWEIDARILVEEGLETREIECGIIGNS
CCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCEEEEEEECCC
ELLTSEIGEVEYGSDWYDYDSKYNSNNKITIPAKIDSKITKQIKEIAIQSCRTLNIFGFA
HHHHHHCCCCCCCCCCCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCEEEEEEEE
RVDFFLEKSSNKIFLNEINTIPGFTKKSMFPMLWEASGLNIEQLVAKLVDISLDL
EEEEEEECCCCEEEEEECCCCCCCCHHCCCCCEEECCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA