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The map label for this gene is miaB [H]

Identifier: 78779785

GI number: 78779785

Start: 1317769

End: 1319160

Strand: Direct

Name: miaB [H]

Synonym: PMT9312_1401

Alternate gene names: 78779785

Gene position: 1317769-1319160 (Clockwise)

Preceding gene: 78779782

Following gene: 78779786

Centisome position: 77.1

GC content: 34.48

Gene sequence:

>1392_bases
GTGCTAACAAAAACAAAAAAAGAAGAAAAAGAATTTCAAAAGGATTCAACTACTAGAAGTTATTGGATAACCACATTTGG
ATGCCAAATGAACAAGGCCGATTCTGAAAGAATGGCTGGAACGTTAGAGAAAATGGGATATACCAGAGCAGATGATGAAC
TAAATGCCGATTTGGTCCTCTACAATACTTGCACTATAAGAGATAATGCGGAACACAAAGTTTATAGTTTCCTAGGAAGA
CAAGCAAAAAGAAAGCACAAAATACCTAGTTTAAAACTTGTTGTCGCAGGTTGCCTTGCTCAGCAAGAAGGAGAGTCCTT
ACTTAGGAGAGTGCCAGAACTTGATCTAGTGATGGGTCCTCAACACGTCAATAACCTTGAGAACCTTCTAGGGAAAGTTG
ATTTAGGTAATCAAGTTGCTGCCACGGAGGAAACCTTCATTTCTGAAGACATAACAAATGCGAGAAGAGAAAGCTCTATT
TGTGGTTGGGTTAATATCATTTATGGGTGTAATGAAAGATGTTCATATTGTGTAGTTCCCTCTGTCAGAGGGAAAGAGCA
ATCAAGATATCCCAATGCTATAAAAAGTGAGATACAAAAATTAGCTGATGATAATTTTAAAGAAATTACTCTTTTGGGGC
AGAATATTGATGCTTATGGAAGAGATCTTCCTGGAACTACAAAAGAGGGGAGAAAAGAAAATACTTTAACTGATCTTTTA
TATTACATTCATGATATTAATGGGATTAGTAGGATTAGGTTTGCTACTAGTCATCCAAGATATTTTTCAAAAAGATTGAT
TCAAGCTTGTTATGAACTTGATAAAGTCTGTGAACATTTCCATATTCCCTTCCAAAGTGGAAATGATGAAATCTTAAAGC
AGATGTCCAGAGGATATAATATTAAAAAGTATAAAAATATAATTGATAATATAAGATCATTAATGCCAGATGCTTCAATT
ACAGCTGACGCTATAGTTGCTTTCCCAGGAGAAACCGAACAACAATATCAAGATACATTGAAACTTATATCAGATATTGG
CTTTGATCAAGTAAATACAGCAGCATACTCCCCAAGGCCAAATACGCCTGCAGCAGTTTGGTCTAATCAACTTTCGGAAG
AAGTAAAAAAAACTAGATTACAGGAAATTAATGCTTTGGTAGAGAAAACTGCTAGGAACAGAAATCAAAGATACATTAAT
AATATTGAAAGCGTTTTAATTGAGGGTTTAAATCCAAAAAATTCCTCGCAAATTATGGGTAGAACCAGGACAAATAGATT
AACTTTCGTAAAGATTCCCAAAAACATTCAATTTAATTTTTCACTTGGAGATGAGATAGATGTCAGAATTAATGAAGCAA
GACCTTTTTCTTTAACAGGTGAACTTTGCTAA

Upstream 100 bases:

>100_bases
ATTCGGGGGTTCGAATCCCCTCGCTCGCCCTCAAAAAATTGCAACAAAATTATTTAACTTGTAATTTTGAATTAAAGAAT
CATAAAAAGTTCCTAGCAAA

Downstream 100 bases:

>100_bases
AAATTTTTTCTAAATGATCGAGGAAAAGAAAAAATGTATTGGATTAATATTTGGTGGATATTCCAATGAGCATGATGTAT
CAATATCCTCTGCTAAGACA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase [H]

Number of amino acids: Translated: 463; Mature: 463

Protein sequence:

>463_residues
MLTKTKKEEKEFQKDSTTRSYWITTFGCQMNKADSERMAGTLEKMGYTRADDELNADLVLYNTCTIRDNAEHKVYSFLGR
QAKRKHKIPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVAATEETFISEDITNARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDINGISRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYNIKKYKNIIDNIRSLMPDASI
TADAIVAFPGETEQQYQDTLKLISDIGFDQVNTAAYSPRPNTPAAVWSNQLSEEVKKTRLQEINALVEKTARNRNQRYIN
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVKIPKNIQFNFSLGDEIDVRINEARPFSLTGELC

Sequences:

>Translated_463_residues
MLTKTKKEEKEFQKDSTTRSYWITTFGCQMNKADSERMAGTLEKMGYTRADDELNADLVLYNTCTIRDNAEHKVYSFLGR
QAKRKHKIPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVAATEETFISEDITNARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDINGISRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYNIKKYKNIIDNIRSLMPDASI
TADAIVAFPGETEQQYQDTLKLISDIGFDQVNTAAYSPRPNTPAAVWSNQLSEEVKKTRLQEINALVEKTARNRNQRYIN
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVKIPKNIQFNFSLGDEIDVRINEARPFSLTGELC
>Mature_463_residues
MLTKTKKEEKEFQKDSTTRSYWITTFGCQMNKADSERMAGTLEKMGYTRADDELNADLVLYNTCTIRDNAEHKVYSFLGR
QAKRKHKIPSLKLVVAGCLAQQEGESLLRRVPELDLVMGPQHVNNLENLLGKVDLGNQVAATEETFISEDITNARRESSI
CGWVNIIYGCNERCSYCVVPSVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
YYIHDINGISRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYNIKKYKNIIDNIRSLMPDASI
TADAIVAFPGETEQQYQDTLKLISDIGFDQVNTAAYSPRPNTPAAVWSNQLSEEVKKTRLQEINALVEKTARNRNQRYIN
NIESVLIEGLNPKNSSQIMGRTRTNRLTFVKIPKNIQFNFSLGDEIDVRINEARPFSLTGELC

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain [H]

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=31.6666666666667, Blast_Score=241, Evalue=1e-63,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=31.6666666666667, Blast_Score=240, Evalue=2e-63,
Organism=Homo sapiens, GI93277076, Length=397, Percent_Identity=28.463476070529, Blast_Score=159, Evalue=7e-39,
Organism=Escherichia coli, GI1786882, Length=452, Percent_Identity=37.3893805309735, Blast_Score=293, Evalue=2e-80,
Organism=Escherichia coli, GI1787057, Length=446, Percent_Identity=25.7847533632287, Blast_Score=144, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17553146, Length=430, Percent_Identity=34.1860465116279, Blast_Score=237, Evalue=9e-63,
Organism=Caenorhabditis elegans, GI71996771, Length=371, Percent_Identity=25.8760107816712, Blast_Score=103, Evalue=2e-22,
Organism=Drosophila melanogaster, GI21356207, Length=477, Percent_Identity=30.6079664570231, Blast_Score=226, Evalue=2e-59,
Organism=Drosophila melanogaster, GI19922432, Length=464, Percent_Identity=25.8620689655172, Blast_Score=145, Evalue=4e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792 [H]

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 52763; Mature: 52763

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS50926 TRAM ; PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKTKKEEKEFQKDSTTRSYWITTFGCQMNKADSERMAGTLEKMGYTRADDELNADLVL
CCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEE
YNTCTIRDNAEHKVYSFLGRQAKRKHKIPSLKLVVAGCLAQQEGESLLRRVPELDLVMGP
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLGKVDLGNQVAATEETFISEDITNARRESSICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
CCCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHH
YYIHDINGISRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYN
HHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IKKYKNIIDNIRSLMPDASITADAIVAFPGETEQQYQDTLKLISDIGFDQVNTAAYSPRP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NTPAAVWSNQLSEEVKKTRLQEINALVEKTARNRNQRYINNIESVLIEGLNPKNSSQIMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHC
RTRTNRLTFVKIPKNIQFNFSLGDEIDVRINEARPFSLTGELC
CCCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLTKTKKEEKEFQKDSTTRSYWITTFGCQMNKADSERMAGTLEKMGYTRADDELNADLVL
CCCCCCHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCEEE
YNTCTIRDNAEHKVYSFLGRQAKRKHKIPSLKLVVAGCLAQQEGESLLRRVPELDLVMGP
EEEEEECCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECC
QHVNNLENLLGKVDLGNQVAATEETFISEDITNARRESSICGWVNIIYGCNERCSYCVVP
HHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEECC
SVRGKEQSRYPNAIKSEIQKLADDNFKEITLLGQNIDAYGRDLPGTTKEGRKENTLTDLL
CCCCCCHHCCCHHHHHHHHHHHCCCCEEEEEECCCCHHCCCCCCCCCCCCCCHHHHHHHH
YYIHDINGISRIRFATSHPRYFSKRLIQACYELDKVCEHFHIPFQSGNDEILKQMSRGYN
HHHHHCCCHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCC
IKKYKNIIDNIRSLMPDASITADAIVAFPGETEQQYQDTLKLISDIGFDQVNTAAYSPRP
HHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCC
NTPAAVWSNQLSEEVKKTRLQEINALVEKTARNRNQRYINNIESVLIEGLNPKNSSQIMG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCHHHHC
RTRTNRLTFVKIPKNIQFNFSLGDEIDVRINEARPFSLTGELC
CCCCCCEEEEECCCCEEEEEECCCEEEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA