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The map label for this gene is thiE [H]

Identifier: 78779752

GI number: 78779752

Start: 1285208

End: 1286305

Strand: Direct

Name: thiE [H]

Synonym: PMT9312_1368

Alternate gene names: 78779752

Gene position: 1285208-1286305 (Clockwise)

Preceding gene: 78779751

Following gene: 78779753

Centisome position: 75.19

GC content: 28.69

Gene sequence:

>1098_bases
ATGCCCTTAAGGTTTTTAAAAACCATAAAAAATAAGTTTCTAATGCAGAATTCAAGCCCAAAAGAACCTGAAGATTTAAG
AATTTTTCAAATTATTGATGCAAATCTTGATAGAGCTCGAGAAGGCTTAAGAGTTCTTGAAGATTGGTCAAGATTTGGGT
TAGGGGAGAATAATTTTGTAACTAAGATAAAAAATTTTAGACAAATTCTAGGTAAAAATCATTTAGAAATTTATAAACAA
TCCAGGAATTACAATGAGGACAAATGTAAAGGGTTGACTCATCAAGAGCAACTCAAAAGAAAAGCTCCTAAGCAAATAAT
AAGTTCCAATGCAGGCAGAGTGCAAGAAGCTTTAAGAGTGATAGAAGAATTCTCAAGACTCCATAATCATGAACTTTCAA
AAATCGCTTCAGAAATTAGATATGAAATTTATAATCTAGAGATTGACTTGTTAAATTTAAGCAAACGTAAAAAGTCGGAT
GAAATATTATACAAAACTCATTTATATGTAATAACAGATCAAAAGAAAAACTTATTAAGAATAATAGAAGATATCTTAAT
TGCAGGGGTAAAAATTATTCAATATAGATTTAAAACAGGAACTGATAAAGATAATCTTGAAGAAGCGATTCAGATTAAGA
ATTTATGTGAAAAATATAATTCATTATTCATCATTAACGATAGGGTTGATATAGCTATTGCATCAAATGCAGATGGTGTT
CATCTCGGACAAGAAGATTTAGACTTAAAAACTGCAAGAAAATTATTAGGGTATTCAAAAATAATTGGCATAAGCGCAAA
TAATGAAAATGATATTTCTAACGCTCTTAAAGAAGGTTGTGATTACATAGGAATAGGACCGGTATTTGAAACTTCAACGA
AAAAAAACAAAAAACCTTTAGGTATTGAAAAGATCAAAACATTAACAAAAGATTTAAATATTCCTTGGTTTGCTATCGGA
GGTGTTACGACAAATAATATTTCATTTTTAAAAAGCCATGGTTTTAAAAAAGTTGCCTTAGTTTCGCAAATAATGAATTC
TGAAGATCCGAAAGAAGACGCTATTATGATCTTAAAAGATTTGTCTAATGAAAATTAA

Upstream 100 bases:

>100_bases
GGTTTGAATTTATCTGTAGAACCAGTTGAAAAGCTGAGATCTCAAATCCAGTTCCAAAATGTAGATCAACTCTCTAATCA
AATTAAAGAAGATAGAGATT

Downstream 100 bases:

>100_bases
GGTAAATGGAGAGGAAAAAAATATTGAACTTGATCAAGAAAATGCTCTACTATCTATAGTGCTAAGTTCCATGGGATATA
AACCCAACACAATTGTTGTT

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase [H]

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MPLRFLKTIKNKFLMQNSSPKEPEDLRIFQIIDANLDRAREGLRVLEDWSRFGLGENNFVTKIKNFRQILGKNHLEIYKQ
SRNYNEDKCKGLTHQEQLKRKAPKQIISSNAGRVQEALRVIEEFSRLHNHELSKIASEIRYEIYNLEIDLLNLSKRKKSD
EILYKTHLYVITDQKKNLLRIIEDILIAGVKIIQYRFKTGTDKDNLEEAIQIKNLCEKYNSLFIINDRVDIAIASNADGV
HLGQEDLDLKTARKLLGYSKIIGISANNENDISNALKEGCDYIGIGPVFETSTKKNKKPLGIEKIKTLTKDLNIPWFAIG
GVTTNNISFLKSHGFKKVALVSQIMNSEDPKEDAIMILKDLSNEN

Sequences:

>Translated_365_residues
MPLRFLKTIKNKFLMQNSSPKEPEDLRIFQIIDANLDRAREGLRVLEDWSRFGLGENNFVTKIKNFRQILGKNHLEIYKQ
SRNYNEDKCKGLTHQEQLKRKAPKQIISSNAGRVQEALRVIEEFSRLHNHELSKIASEIRYEIYNLEIDLLNLSKRKKSD
EILYKTHLYVITDQKKNLLRIIEDILIAGVKIIQYRFKTGTDKDNLEEAIQIKNLCEKYNSLFIINDRVDIAIASNADGV
HLGQEDLDLKTARKLLGYSKIIGISANNENDISNALKEGCDYIGIGPVFETSTKKNKKPLGIEKIKTLTKDLNIPWFAIG
GVTTNNISFLKSHGFKKVALVSQIMNSEDPKEDAIMILKDLSNEN
>Mature_364_residues
PLRFLKTIKNKFLMQNSSPKEPEDLRIFQIIDANLDRAREGLRVLEDWSRFGLGENNFVTKIKNFRQILGKNHLEIYKQS
RNYNEDKCKGLTHQEQLKRKAPKQIISSNAGRVQEALRVIEEFSRLHNHELSKIASEIRYEIYNLEIDLLNLSKRKKSDE
ILYKTHLYVITDQKKNLLRIIEDILIAGVKIIQYRFKTGTDKDNLEEAIQIKNLCEKYNSLFIINDRVDIAIASNADGVH
LGQEDLDLKTARKLLGYSKIIGISANNENDISNALKEGCDYIGIGPVFETSTKKNKKPLGIEKIKTLTKDLNIPWFAIGG
VTTNNISFLKSHGFKKVALVSQIMNSEDPKEDAIMILKDLSNEN

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP) [H]

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family [H]

Homologues:

Organism=Escherichia coli, GI1790426, Length=190, Percent_Identity=31.0526315789474, Blast_Score=93, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325042, Length=206, Percent_Identity=37.378640776699, Blast_Score=120, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR016229
- InterPro:   IPR003733 [H]

Pfam domain/function: PF02581 TMP-TENI [H]

EC number: =2.5.1.3 [H]

Molecular weight: Translated: 41902; Mature: 41771

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLRFLKTIKNKFLMQNSSPKEPEDLRIFQIIDANLDRAREGLRVLEDWSRFGLGENNFV
CCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHH
TKIKNFRQILGKNHLEIYKQSRNYNEDKCKGLTHQEQLKRKAPKQIISSNAGRVQEALRV
HHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
IEEFSRLHNHELSKIASEIRYEIYNLEIDLLNLSKRKKSDEILYKTHLYVITDQKKNLLR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEEEEEECCHHHHHH
IIEDILIAGVKIIQYRFKTGTDKDNLEEAIQIKNLCEKYNSLFIINDRVDIAIASNADGV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCC
HLGQEDLDLKTARKLLGYSKIIGISANNENDISNALKEGCDYIGIGPVFETSTKKNKKPL
CCCCHHCCHHHHHHHHCHHHEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCC
GIEKIKTLTKDLNIPWFAIGGVTTNNISFLKSHGFKKVALVSQIMNSEDPKEDAIMILKD
CHHHHHHHHHHCCCCEEEECCEECCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHH
LSNEN
CCCCC
>Mature Secondary Structure 
PLRFLKTIKNKFLMQNSSPKEPEDLRIFQIIDANLDRAREGLRVLEDWSRFGLGENNFV
CHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHH
TKIKNFRQILGKNHLEIYKQSRNYNEDKCKGLTHQEQLKRKAPKQIISSNAGRVQEALRV
HHHHHHHHHHCCCHHHHHHHHCCCCHHHHCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHH
IEEFSRLHNHELSKIASEIRYEIYNLEIDLLNLSKRKKSDEILYKTHLYVITDQKKNLLR
HHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCEEEEEEEEEEECCHHHHHH
IIEDILIAGVKIIQYRFKTGTDKDNLEEAIQIKNLCEKYNSLFIINDRVDIAIASNADGV
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCEEEEECCEEEEEECCCCCC
HLGQEDLDLKTARKLLGYSKIIGISANNENDISNALKEGCDYIGIGPVFETSTKKNKKPL
CCCCHHCCHHHHHHHHCHHHEEEEECCCCHHHHHHHHHCCCEECCCCCCCCCCCCCCCCC
GIEKIKTLTKDLNIPWFAIGGVTTNNISFLKSHGFKKVALVSQIMNSEDPKEDAIMILKD
CHHHHHHHHHHCCCCEEEECCEECCCHHHHHHCCCHHHHHHHHHHCCCCCCHHHHHHHHH
LSNEN
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]