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The map label for this gene is cobQ

Identifier: 78779642

GI number: 78779642

Start: 1171009

End: 1172538

Strand: Direct

Name: cobQ

Synonym: PMT9312_1259

Alternate gene names: 78779642

Gene position: 1171009-1172538 (Clockwise)

Preceding gene: 78779640

Following gene: 78779643

Centisome position: 68.51

GC content: 31.63

Gene sequence:

>1530_bases
ATGTATTTAGAAGAGAAATTACATAAAATAAAGAAACCAATAATGGTCCTAGGTACTTCTAGTGGAGCAGGAAAATCATT
AACGGTTACAGCTATTTGCAGGATTCTTAAAAATTTAGCTGAACAGCCAATACCTTTTAAAGGACAAAACATGAGTAACA
ATGCATGGGTTGACTGGGAAGGGGGAGAGATGGCATATTCACAAGCACTTCAGGCCTTCGCTTGTGGTGTCAATCCTTCT
TCAGAAATGAACCCCATTTTATTAAAACCACAAGGCAATTCAGTAAGCGAGGTAATTCACCTTGGCAAAAGTATAGGGAC
CACAAGCGCGAAAAATTACTACCAAGATTGGTTTATTCCCGGATGGGAAGTAATTAAAAAAAGTTTAAACTCTATTTATA
AAAAAAATCCAAATTGTCGTTTAATTATAGAAGGGGCTGGCAGTCCAGTAGAAATGAATTTAAAACATAGAGACCTTACT
AATTTAAAAATTGCAAAGTATTTAAAGGCAAATTGCATTTTAGTTACTGATATTGAAAGGGGAGGAGTATTTGCACAAAT
AATTGGAACCCTTGAATTAATGAAACCTGAAGAAAAAAAACTTATAAAGGGAATTATAATAAATAGATTCAGAGGAGACC
TTTCATTATTTGAAGAAGGGAAAAAATGGATAGAAGAAAAAACTCAAATACCTGTTATTGGAATTGTTCCATGGCTAAAT
GATTCATTCCCTCCAGAAGATTCTTTAGATTTATTAGAAAATAAATCTCGTTTCACAAATGCTGAGATCAAAGTTGGAAT
TATAAAATTACCATCTATAAGTAACTTCTCAGATTTTGATCCATTAGAAAATGAAGAATCAATATTAATTGAATGGATTA
GAGAATCGCAAAACCTTAAGAAGTATGACTTTATTATTCTGCCTGGTAGTAAACAAACTATTAAAGATCAAATGTTTCTT
GAAAAGACTGGTTTATCTCATGATTTGAGGGAATATTCAAATAATAAGGGTCACATTATTGGGATCTGTGGAGGTTTACA
AATGTTAGGCACATTTCTTGAAGATCCTTTTTTAAAAGAGGGCTCGAAAACTTTCTCAGAACAAAAAATTAAAGGCATTG
GATTACTGCCATTAAGAACCACTTTCTTTGAAGAAAAATTAACCCGTCAAATCAGTTCCGAATCTTTATGGCCATGCCAA
TCTAAAATTAATGGTTTTGAAATTCACAATGGCCAGACCGAATTAGATAATACTCAAAACTCATTAAAGATTAACCCTAT
CTTCAAAGACTTAAATTTAGGTTGGTACAAAGAAAATAAAGAAGGTGGGACAATTGCAGGTACATACATTCACGGGATCT
TTGAAAATGATATTTGGAGAGATCAATATATAAATTTGATAAGAATGAACAAGAAATTGCCAGCATTAAAAAAAAGAACA
AGCTCTTATAAAAACAAGAGAGAAAAAATAATTGAAAATCTAGCAAATGAATTTAATAAGCATTTAAACCTCTCATCATT
ATTAAATTGA

Upstream 100 bases:

>100_bases
CTAGATTGAGAAGCAGAGGCTAGAATTAACACAAATTTTTTTTATCAAATTATAATTCAATTTACAAATTAATAAATTTC
AAAATTAATATAAATTACGA

Downstream 100 bases:

>100_bases
AAAATTCAAAAATTAAAATTATTTGGCCAAATAATATTGAAACATATGTTTCTGAAGGGGAAGATTGGTTCTCTACTGCA
AAAAAAGCAGGTTTAGAAAT

Product: cobyric acid synthase

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 509

Protein sequence:

>509_residues
MYLEEKLHKIKKPIMVLGTSSGAGKSLTVTAICRILKNLAEQPIPFKGQNMSNNAWVDWEGGEMAYSQALQAFACGVNPS
SEMNPILLKPQGNSVSEVIHLGKSIGTTSAKNYYQDWFIPGWEVIKKSLNSIYKKNPNCRLIIEGAGSPVEMNLKHRDLT
NLKIAKYLKANCILVTDIERGGVFAQIIGTLELMKPEEKKLIKGIIINRFRGDLSLFEEGKKWIEEKTQIPVIGIVPWLN
DSFPPEDSLDLLENKSRFTNAEIKVGIIKLPSISNFSDFDPLENEESILIEWIRESQNLKKYDFIILPGSKQTIKDQMFL
EKTGLSHDLREYSNNKGHIIGICGGLQMLGTFLEDPFLKEGSKTFSEQKIKGIGLLPLRTTFFEEKLTRQISSESLWPCQ
SKINGFEIHNGQTELDNTQNSLKINPIFKDLNLGWYKENKEGGTIAGTYIHGIFENDIWRDQYINLIRMNKKLPALKKRT
SSYKNKREKIIENLANEFNKHLNLSSLLN

Sequences:

>Translated_509_residues
MYLEEKLHKIKKPIMVLGTSSGAGKSLTVTAICRILKNLAEQPIPFKGQNMSNNAWVDWEGGEMAYSQALQAFACGVNPS
SEMNPILLKPQGNSVSEVIHLGKSIGTTSAKNYYQDWFIPGWEVIKKSLNSIYKKNPNCRLIIEGAGSPVEMNLKHRDLT
NLKIAKYLKANCILVTDIERGGVFAQIIGTLELMKPEEKKLIKGIIINRFRGDLSLFEEGKKWIEEKTQIPVIGIVPWLN
DSFPPEDSLDLLENKSRFTNAEIKVGIIKLPSISNFSDFDPLENEESILIEWIRESQNLKKYDFIILPGSKQTIKDQMFL
EKTGLSHDLREYSNNKGHIIGICGGLQMLGTFLEDPFLKEGSKTFSEQKIKGIGLLPLRTTFFEEKLTRQISSESLWPCQ
SKINGFEIHNGQTELDNTQNSLKINPIFKDLNLGWYKENKEGGTIAGTYIHGIFENDIWRDQYINLIRMNKKLPALKKRT
SSYKNKREKIIENLANEFNKHLNLSSLLN
>Mature_509_residues
MYLEEKLHKIKKPIMVLGTSSGAGKSLTVTAICRILKNLAEQPIPFKGQNMSNNAWVDWEGGEMAYSQALQAFACGVNPS
SEMNPILLKPQGNSVSEVIHLGKSIGTTSAKNYYQDWFIPGWEVIKKSLNSIYKKNPNCRLIIEGAGSPVEMNLKHRDLT
NLKIAKYLKANCILVTDIERGGVFAQIIGTLELMKPEEKKLIKGIIINRFRGDLSLFEEGKKWIEEKTQIPVIGIVPWLN
DSFPPEDSLDLLENKSRFTNAEIKVGIIKLPSISNFSDFDPLENEESILIEWIRESQNLKKYDFIILPGSKQTIKDQMFL
EKTGLSHDLREYSNNKGHIIGICGGLQMLGTFLEDPFLKEGSKTFSEQKIKGIGLLPLRTTFFEEKLTRQISSESLWPCQ
SKINGFEIHNGQTELDNTQNSLKINPIFKDLNLGWYKENKEGGTIAGTYIHGIFENDIWRDQYINLIRMNKKLPALKKRT
SSYKNKREKIIENLANEFNKHLNLSSLLN

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_PROM9 (Q319X7)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397754.1
- ProteinModelPortal:   Q319X7
- STRING:   Q319X7
- GeneID:   3766066
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1259
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- ProtClustDB:   PRK00784
- BioCyc:   PMAR74546:PMT9312_1259-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 57798; Mature: 57798

Theoretical pI: Translated: 9.01; Mature: 9.01

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 343-343 ACT_SITE 451-451

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYLEEKLHKIKKPIMVLGTSSGAGKSLTVTAICRILKNLAEQPIPFKGQNMSNNAWVDWE
CCHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
GGEMAYSQALQAFACGVNPSSEMNPILLKPQGNSVSEVIHLGKSIGTTSAKNYYQDWFIP
CCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCC
GWEVIKKSLNSIYKKNPNCRLIIEGAGSPVEMNLKHRDLTNLKIAKYLKANCILVTDIER
CHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEECCC
GGVFAQIIGTLELMKPEEKKLIKGIIINRFRGDLSLFEEGKKWIEEKTQIPVIGIVPWLN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
DSFPPEDSLDLLENKSRFTNAEIKVGIIKLPSISNFSDFDPLENEESILIEWIRESQNLK
CCCCCCCCHHHHHCCHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
KYDFIILPGSKQTIKDQMFLEKTGLSHDLREYSNNKGHIIGICGGLQMLGTFLEDPFLKE
EEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECHHHHHHHHHCCHHHHC
GSKTFSEQKIKGIGLLPLRTTFFEEKLTRQISSESLWPCQSKINGFEIHNGQTELDNTQN
CHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCCCCCC
SLKINPIFKDLNLGWYKENKEGGTIAGTYIHGIFENDIWRDQYINLIRMNKKLPALKKRT
CEEEEEEHCCCCCCEEEECCCCCCEEEEEEEEHHHCCCCHHHHHHHHHHCCCCHHHHHHH
SSYKNKREKIIENLANEFNKHLNLSSLLN
HHHHHHHHHHHHHHHHHHHHCCCHHHHCC
>Mature Secondary Structure
MYLEEKLHKIKKPIMVLGTSSGAGKSLTVTAICRILKNLAEQPIPFKGQNMSNNAWVDWE
CCHHHHHHHHCCCEEEEECCCCCCCEEHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
GGEMAYSQALQAFACGVNPSSEMNPILLKPQGNSVSEVIHLGKSIGTTSAKNYYQDWFIP
CCHHHHHHHHHHHHCCCCCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCHHHHHHHCCCC
GWEVIKKSLNSIYKKNPNCRLIIEGAGSPVEMNLKHRDLTNLKIAKYLKANCILVTDIER
CHHHHHHHHHHHHHCCCCEEEEEECCCCCEEEEECCCCCCCHHHHHHHCCCEEEEEECCC
GGVFAQIIGTLELMKPEEKKLIKGIIINRFRGDLSLFEEGKKWIEEKTQIPVIGIVPWLN
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCEEEEECCCC
DSFPPEDSLDLLENKSRFTNAEIKVGIIKLPSISNFSDFDPLENEESILIEWIRESQNLK
CCCCCCCCHHHHHCCHHCCCCEEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
KYDFIILPGSKQTIKDQMFLEKTGLSHDLREYSNNKGHIIGICGGLQMLGTFLEDPFLKE
EEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHCCCCCEEEEEECHHHHHHHHHCCHHHHC
GSKTFSEQKIKGIGLLPLRTTFFEEKLTRQISSESLWPCQSKINGFEIHNGQTELDNTQN
CHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEECCCCCCCCCCC
SLKINPIFKDLNLGWYKENKEGGTIAGTYIHGIFENDIWRDQYINLIRMNKKLPALKKRT
CEEEEEEHCCCCCCEEEECCCCCCEEEEEEEEHHHCCCCHHHHHHHHHHCCCCHHHHHHH
SSYKNKREKIIENLANEFNKHLNLSSLLN
HHHHHHHHHHHHHHHHHHHHCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA