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The map label for this gene is cobB [H]

Identifier: 78779466

GI number: 78779466

Start: 993308

End: 994699

Strand: Reverse

Name: cobB [H]

Synonym: PMT9312_1083

Alternate gene names: 78779466

Gene position: 994699-993308 (Counterclockwise)

Preceding gene: 78779467

Following gene: 78779462

Centisome position: 58.2

GC content: 29.96

Gene sequence:

>1392_bases
ATGCCTTGTGTAATATCTTCTCCTTCAACTGATAGTGGGAAAACTACATTATCGCTTTTGATATCTTGTTGGGCGTTTTC
AAAAGGTATTAAGATACAAACTTTCAAGGTTGGCCCAGATTATCTTGATCAACAACAACTTAGTTCAATTGGTCAACCTA
TTTGTAGGAATTTAGATATTTTTTTAAGTGGTGAGGCATGGGTTCAAGAAAGTTTTTTTAAACATTCTTTGAAATATGAA
TTCTCATTAATTGAAGGGGCAATGGGCTTATTTGATGGATTAGGTTCAACAACCTATTCAAGTACAGCAAATATCTCTAA
ACTTCTCAATGCTCCAGTAATTTTTATTGTTAATGCTAGAGGTCAAGTAGCTTCTCTTTTGGCGACTATTCGAGGTTTTA
GAGATTTCGATAGTGAGTTGTCAATAGTAGGAATTATATTTAACAACGTTAATTCAGATAGACATAAAAAATTAATAGAA
GAGGTTTTCAAAAATGAAGATATCGAAATTCTTGGTTTTTTACCTTCTGATTCAAACATAACTTTAAACAAAGCTAATTT
AGGTTTGATATCTCCATTGGATAATGGTAAAGAAATTGATGTTGAATATTTTGCAAATTTCGCCGAAAGAAATCTTGATG
TATTTTCTCTTATTAAATTCTTGAAATCTCCTCAAAAGAAAATATTTAATTCTGTTAGTTTTAAAGATTTTAAAATTGAT
AAAACTAAACCTATCGCAATTGCAGAAGATAAAATCTTTCACTTTCAATACCCTGAAACTAAGGAGTTTTTGAGTGAAAT
AGGAATACCTTTGATTTCATGGAGTATTTATGAAGATCAAGAAATACCTAATGAAGCTTCTTCTTTAATTATTCCGGGGG
GATTTCCTGAAAAATATGCTGATCATATAAGTAATTCTAGGAAAAGTTTAAATTCGTTAAGGAAATTCCGCAAAAATGGA
TTTATATATGCAGAGTGCGGAGGGATGATGATATTAGGAGACTTCATAAAAGATGAAAATGGTAATTATCATAAAATGAG
TGGAATCCTACCTTTTAGATCGAAAAAACGTAAGCTTTCAGTAGGGTATAGATACATTAAGGGTTTAAAAGATACTCCGA
TCATTAAACAAAATCAATTAATTAGAGGACATGAATTTCATTATTGGGAAATTGAAAATAATTTATTTGAATTTGATTTA
AAAAAAGATGAGCCTCAGAAAAAACTCTCTCCTCCATGGAAAATTAAATCTTGGGAAACTAAATATAAAAATGAAGGCTT
TTTTGATAACAAATTACATGCAAGTTGGATTCACTTACATTTTCCAAGTTCTCCAGAAGTTGCAAAAAACTTTATAGATG
CTACCCAAATTGCTTATTCAAAGGATTCTTAA

Upstream 100 bases:

>100_bases
TAGTAAATTATTGGGAAGTAGTAGAGGTAATACAAGTCCTCTTTTTGAAAATTCTATTAAAATAGCTCTTCAAATATTTA
ATGGTTTTAATAACTAATAA

Downstream 100 bases:

>100_bases
TTTATTATCAAATCAAATATCTTGAGAGGGGAACCTAAAAAAAATATTCCAGGCAAAGTAATTAATGGTCCTAAAAAACT
ACCCACCATAACTTCAATTT

Product: hydrogenobyrinic acid a,c-diamide synthase (glutamine-hydrolysing) / cobyrinate a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MPCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLSGEAWVQESFFKHSLKYE
FSLIEGAMGLFDGLGSTTYSSTANISKLLNAPVIFIVNARGQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIE
EVFKNEDIEILGFLPSDSNITLNKANLGLISPLDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKIFNSVSFKDFKID
KTKPIAIAEDKIFHFQYPETKEFLSEIGIPLISWSIYEDQEIPNEASSLIIPGGFPEKYADHISNSRKSLNSLRKFRKNG
FIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKRKLSVGYRYIKGLKDTPIIKQNQLIRGHEFHYWEIENNLFEFDL
KKDEPQKKLSPPWKIKSWETKYKNEGFFDNKLHASWIHLHFPSSPEVAKNFIDATQIAYSKDS

Sequences:

>Translated_463_residues
MPCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLSGEAWVQESFFKHSLKYE
FSLIEGAMGLFDGLGSTTYSSTANISKLLNAPVIFIVNARGQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIE
EVFKNEDIEILGFLPSDSNITLNKANLGLISPLDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKIFNSVSFKDFKID
KTKPIAIAEDKIFHFQYPETKEFLSEIGIPLISWSIYEDQEIPNEASSLIIPGGFPEKYADHISNSRKSLNSLRKFRKNG
FIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKRKLSVGYRYIKGLKDTPIIKQNQLIRGHEFHYWEIENNLFEFDL
KKDEPQKKLSPPWKIKSWETKYKNEGFFDNKLHASWIHLHFPSSPEVAKNFIDATQIAYSKDS
>Mature_462_residues
PCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDIFLSGEAWVQESFFKHSLKYEF
SLIEGAMGLFDGLGSTTYSSTANISKLLNAPVIFIVNARGQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIEE
VFKNEDIEILGFLPSDSNITLNKANLGLISPLDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKIFNSVSFKDFKIDK
TKPIAIAEDKIFHFQYPETKEFLSEIGIPLISWSIYEDQEIPNEASSLIIPGGFPEKYADHISNSRKSLNSLRKFRKNGF
IYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKRKLSVGYRYIKGLKDTPIIKQNQLIRGHEFHYWEIENNLFEFDLK
KDEPQKKLSPPWKIKSWETKYKNEGFFDNKLHASWIHLHFPSSPEVAKNFIDATQIAYSKDS

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 52762; Mature: 52631

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00037 MYB_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDI
CCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEE
FLSGEAWVQESFFKHSLKYEFSLIEGAMGLFDGLGSTTYSSTANISKLLNAPVIFIVNAR
EECCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCC
GQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIEEVFKNEDIEILGFLPSDSNI
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCE
TLNKANLGLISPLDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKIFNSVSFKDFKID
EEEECCCEEECCCCCCCEECHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCCEEECC
KTKPIAIAEDKIFHFQYPETKEFLSEIGIPLISWSIYEDQEIPNEASSLIIPGGFPEKYA
CCCCEEEECCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCCHHHH
DHISNSRKSLNSLRKFRKNGFIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKRKLS
HHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEHHHCCCCCEEEECCCCCCCCCCHHHH
VGYRYIKGLKDTPIIKQNQLIRGHEFHYWEIENNLFEFDLKKDEPQKKLSPPWKIKSWET
HHHHHHCCCCCCCCCCCCCEECCCCEEEEEECCCEEEEECCCCCCHHHCCCCCCCCCCCC
KYKNEGFFDNKLHASWIHLHFPSSPEVAKNFIDATQIAYSKDS
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure 
PCVISSPSTDSGKTTLSLLISCWAFSKGIKIQTFKVGPDYLDQQQLSSIGQPICRNLDI
CCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHCHHHHCCCEE
FLSGEAWVQESFFKHSLKYEFSLIEGAMGLFDGLGSTTYSSTANISKLLNAPVIFIVNAR
EECCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCEEEEECCC
GQVASLLATIRGFRDFDSELSIVGIIFNNVNSDRHKKLIEEVFKNEDIEILGFLPSDSNI
CHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHCCCCEEEEEEECCCCCE
TLNKANLGLISPLDNGKEIDVEYFANFAERNLDVFSLIKFLKSPQKKIFNSVSFKDFKID
EEEECCCEEECCCCCCCEECHHHHHHHHHCCCCHHHHHHHHHCHHHHHHHCCCCCEEECC
KTKPIAIAEDKIFHFQYPETKEFLSEIGIPLISWSIYEDQEIPNEASSLIIPGGFPEKYA
CCCCEEEECCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCCCCCEEECCCCCHHHH
DHISNSRKSLNSLRKFRKNGFIYAECGGMMILGDFIKDENGNYHKMSGILPFRSKKRKLS
HHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEEEHHHCCCCCEEEECCCCCCCCCCHHHH
VGYRYIKGLKDTPIIKQNQLIRGHEFHYWEIENNLFEFDLKKDEPQKKLSPPWKIKSWET
HHHHHHCCCCCCCCCCCCCEECCCCEEEEEECCCEEEEECCCCCCHHHCCCCCCCCCCCC
KYKNEGFFDNKLHASWIHLHFPSSPEVAKNFIDATQIAYSKDS
CCCCCCCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA