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The map label for this gene is hisH

Identifier: 78779454

GI number: 78779454

Start: 979143

End: 979760

Strand: Reverse

Name: hisH

Synonym: PMT9312_1071

Alternate gene names: 78779454

Gene position: 979760-979143 (Counterclockwise)

Preceding gene: 78779455

Following gene: 78779453

Centisome position: 57.32

GC content: 30.42

Gene sequence:

>618_bases
TTGCATAAAATTGGACTTATAGACTATGGAATGGGTAATATTCATTCAGTAACAAAATCTCTAGAAAGTCTTGGAGAAGA
AATAATTTTAATTAAAAACTTTAGTGAATCAAAAGCTTGTAAGGCGATAATACTTCCTGGAGTTGGAGCATTTGATCCTG
CGATGATTAATCTTATAAATACTGATTTGATAATTGATTTGAAAAATTGGATTAATAGTGGTAAGTCTTTTTTAGGAATT
TGTTTAGGTCTTCAACTTCTTTTTGAATCTAGTGATGAAGGAAAAGTTCAAGGACTAGGTATTTTAAAGGGCAAAATTCA
AAAAATACCCAATATAGTTAATCAAAGAATCCCCCACATGGGTTGGTGCGAACTTCTACCTACAAAAACAAATACTTTAT
TAGAACTAGAGGAATTAAATAATTGGGTCTATTTTGTCCATTCCTATCATGCAATCCCAGATGACTTCAGTATTATTGCA
GCTCAGGTTAATTATGGTTCTGAAAAATTAACAGCTATGATTGAAAATGATAATTTATTAGCTTGTCAATTTCATCCTGA
GAAATCTGGTAAAACTGGAGAAAAACTTTTGAGAAGATGGCTTAGTAATATTCAATAA

Upstream 100 bases:

>100_bases
ATTAATGATCTTTAAAGGAGGTCAGAAAGTTGATACTGTGGTTGGAGCCGTACCAAAAGCAACTCTTTCGAGCACTTTAA
CTAAGCATCTATAAATAGCT

Downstream 100 bases:

>100_bases
CTGATAATTACTGATGAAGACAAATTTAAGATTAATAGGTGGTAAAAAACTCCAAAGTCCAAATAATATTTATACAAGAC
CTACAACTTTGAGAGTTAGA

Product: imidazole glycerol phosphate synthase subunit HisH

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFSESKACKAIILPGVGAFDPAMINLINTDLIIDLKNWINSGKSFLGI
CLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHMGWCELLPTKTNTLLELEELNNWVYFVHSYHAIPDDFSIIA
AQVNYGSEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ

Sequences:

>Translated_205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFSESKACKAIILPGVGAFDPAMINLINTDLIIDLKNWINSGKSFLGI
CLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHMGWCELLPTKTNTLLELEELNNWVYFVHSYHAIPDDFSIIA
AQVNYGSEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ
>Mature_205_residues
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFSESKACKAIILPGVGAFDPAMINLINTDLIIDLKNWINSGKSFLGI
CLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHMGWCELLPTKTNTLLELEELNNWVYFVHSYHAIPDDFSIIA
AQVNYGSEKLTAMIENDNLLACQFHPEKSGKTGEKLLRRWLSNIQ

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=197, Percent_Identity=35.5329949238579, Blast_Score=108, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6319725, Length=215, Percent_Identity=35.3488372093023, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_PROM9 (Q31AG5)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397566.1
- ProteinModelPortal:   Q31AG5
- SMR:   Q31AG5
- STRING:   Q31AG5
- GeneID:   3765874
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1071
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- ProtClustDB:   PRK13141
- BioCyc:   PMAR74546:PMT9312_1071-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22875; Mature: 22875

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 81-81 ACT_SITE 185-185 ACT_SITE 187-187

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFSESKACKAIILPGVGAFDPAMINLIN
CCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHC
TDLIIDLKNWINSGKSFLGICLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHM
CCEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCC
GWCELLPTKTNTLLELEELNNWVYFVHSYHAIPDDFSIIAAQVNYGSEKLTAMIENDNLL
CCCEECCCCCCCEEEHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCEE
ACQFHPEKSGKTGEKLLRRWLSNIQ
EEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MHKIGLIDYGMGNIHSVTKSLESLGEEIILIKNFSESKACKAIILPGVGAFDPAMINLIN
CCCEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHC
TDLIIDLKNWINSGKSFLGICLGLQLLFESSDEGKVQGLGILKGKIQKIPNIVNQRIPHM
CCEEEEEHHHHCCCHHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCC
GWCELLPTKTNTLLELEELNNWVYFVHSYHAIPDDFSIIAAQVNYGSEKLTAMIENDNLL
CCCEECCCCCCCEEEHHHCCCEEEEEEEECCCCCCCEEEEEEECCCCCEEEEEEECCCEE
ACQFHPEKSGKTGEKLLRRWLSNIQ
EEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA