Click here to switch to the map view.

The map label for this gene is dus1 [H]

Identifier: 78779403

GI number: 78779403

Start: 940333

End: 941340

Strand: Direct

Name: dus1 [H]

Synonym: PMT9312_1019

Alternate gene names: 78779403

Gene position: 940333-941340 (Clockwise)

Preceding gene: 78779400

Following gene: 78779404

Centisome position: 55.02

GC content: 32.24

Gene sequence:

>1008_bases
ATGTCTTCAGATATAAAGATAAAAGGAAGGGGAGTTAACAGGATAATTAAGAGTAAGGTGATGCTATCGCCCTTAGCAGG
GGTTACAGATAACATTTTTAGGCGACTTGTTCGTAAATGGGCTCCTAACTCTTTACTTTTTACAGAAATGATAAATGCCA
CGAGTCTTAAAAAAGGATTTGGAACACAAAAAATCAATCAAATAAATTTAGAAGAAGGTCCAGTAGGAGTTCAAATATTT
GATAATAGACCATATGCTGTTTCTGAAGCCGCTAAACAAGCTGAGGACTCTGGAGCTTTCTTAATTGATATAAATATGGG
ATGTCCAGTAAAAAAGATTGCAAAGAAAGGTGGAGGCAGTGCCTTAATTAAAGACCGTAAACTTGCTATAGAATTAGTCA
AAAATGTCGTAAAAGCTGTAAAAATTCCAGTTACAGTAAAAACTCGACTCGGATGGGATAGTAAAGAAGAAAATATAGAA
GATTTTTTATTTAAGCTTCAAGATGCGGGAGCAACAATGATCACACTTCATGGAAGAACTAGAAAACAAGGTTTTTCAGG
TAAATCAGATTGGGAAATGATCGGGAGACTTAAAAAGTTATTGGAAATTCCAGTAATTGCTAATGGAGATATCAAAAATC
CAGATGACGCTCTTAATTGTTTAAATAAAACAAATGCAGATGGTGTAATGATTGGACGAGGGATTTTAGGATCTCCATGG
AAATTAGGAGAAATAGATTATGCCATTAAAGAAAATAAAAATTTTAAAGAACCAAACGCAGAAGAAAAACTATATTTAAT
TATTGAGCACCTTGATGAATTAATAAAAGAAAAAGGAGATCACGGTTTGCTAATTGCTAGGAAGCATATTTCATGGACAT
GCAAAGATTTTAAGGGTGCATCAAATTTGCGAAATAACCTGGTTAGAGCTGTTGATAAAAATGAAGTTAAAAATTTAATA
AATAAAATGATTAAAACTTTGAATAAAGAAAAAAATAGATTAGCTTAA

Upstream 100 bases:

>100_bases
AAATAAAATCACTTATCAAAAAATTAAATATATAAAATAATAAAGATAGAGTAAACTCCATTTCAGAATTTATAATAAAT
TAAAAAATATTTAAAATAAT

Downstream 100 bases:

>100_bases
AACAACATTATTCTTCCAATGAAAATAATTAGTAAAGAAACAAGTAAAAGAGTTTGTGATCATATGAACAATGATCATAT
TGATTCAGTTCACAAATATC

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MSSDIKIKGRGVNRIIKSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGFGTQKINQINLEEGPVGVQIF
DNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVKIPVTVKTRLGWDSKEENIE
DFLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLNKTNADGVMIGRGILGSPW
KLGEIDYAIKENKNFKEPNAEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLI
NKMIKTLNKEKNRLA

Sequences:

>Translated_335_residues
MSSDIKIKGRGVNRIIKSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGFGTQKINQINLEEGPVGVQIF
DNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVKIPVTVKTRLGWDSKEENIE
DFLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLNKTNADGVMIGRGILGSPW
KLGEIDYAIKENKNFKEPNAEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLI
NKMIKTLNKEKNRLA
>Mature_334_residues
SSDIKIKGRGVNRIIKSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGFGTQKINQINLEEGPVGVQIFD
NRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGSALIKDRKLAIELVKNVVKAVKIPVTVKTRLGWDSKEENIED
FLFKLQDAGATMITLHGRTRKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLNKTNADGVMIGRGILGSPWK
LGEIDYAIKENKNFKEPNAEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGASNLRNNLVRAVDKNEVKNLIN
KMIKTLNKEKNRLA

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=252, Percent_Identity=30.5555555555556, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI239788462, Length=236, Percent_Identity=31.3559322033898, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI40807366, Length=243, Percent_Identity=30.0411522633745, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI31742496, Length=223, Percent_Identity=33.6322869955157, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI8923374, Length=142, Percent_Identity=32.3943661971831, Blast_Score=72, Evalue=7e-13,
Organism=Escherichia coli, GI1789660, Length=302, Percent_Identity=34.1059602649007, Blast_Score=177, Evalue=8e-46,
Organism=Escherichia coli, GI1788462, Length=302, Percent_Identity=33.112582781457, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI145693211, Length=245, Percent_Identity=31.0204081632653, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17507177, Length=237, Percent_Identity=34.1772151898734, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI25144369, Length=252, Percent_Identity=29.3650793650794, Blast_Score=96, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17543114, Length=240, Percent_Identity=28.75, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323560, Length=244, Percent_Identity=32.3770491803279, Blast_Score=101, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6323433, Length=303, Percent_Identity=26.0726072607261, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323437, Length=226, Percent_Identity=29.646017699115, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585320, Length=238, Percent_Identity=33.6134453781513, Blast_Score=119, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24580595, Length=225, Percent_Identity=30.6666666666667, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19920448, Length=225, Percent_Identity=30.6666666666667, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19921524, Length=231, Percent_Identity=31.6017316017316, Blast_Score=91, Evalue=7e-19,
Organism=Drosophila melanogaster, GI45549423, Length=136, Percent_Identity=34.5588235294118, Blast_Score=75, Evalue=8e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37367; Mature: 37236

Theoretical pI: Translated: 10.43; Mature: 10.43

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDIKIKGRGVNRIIKSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGF
CCCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
GTQKINQINLEEGPVGVQIFDNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGS
CCCCCCEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCC
ALIKDRKLAIELVKNVVKAVKIPVTVKTRLGWDSKEENIEDFLFKLQDAGATMITLHGRT
EEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCH
RKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLNKTNADGVMIGRGILGSPW
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCC
KLGEIDYAIKENKNFKEPNAEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGA
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHCCH
SNLRNNLVRAVDKNEVKNLINKMIKTLNKEKNRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SSDIKIKGRGVNRIIKSKVMLSPLAGVTDNIFRRLVRKWAPNSLLFTEMINATSLKKGF
CCCEEEECCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
GTQKINQINLEEGPVGVQIFDNRPYAVSEAAKQAEDSGAFLIDINMGCPVKKIAKKGGGS
CCCCCCEECCCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCHHHHHHCCCCC
ALIKDRKLAIELVKNVVKAVKIPVTVKTRLGWDSKEENIEDFLFKLQDAGATMITLHGRT
EEHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCEEEEECCCH
RKQGFSGKSDWEMIGRLKKLLEIPVIANGDIKNPDDALNCLNKTNADGVMIGRGILGSPW
HCCCCCCCCHHHHHHHHHHHHHCCCEECCCCCCHHHHHHHHHCCCCCCEEEECCCCCCCC
KLGEIDYAIKENKNFKEPNAEEKLYLIIEHLDELIKEKGDHGLLIARKHISWTCKDFKGA
CCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEEECCCEEEEHHHCCH
SNLRNNLVRAVDKNEVKNLINKMIKTLNKEKNRLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]