Click here to switch to the map view.

The map label for this gene is purN [H]

Identifier: 78779293

GI number: 78779293

Start: 840205

End: 840939

Strand: Reverse

Name: purN [H]

Synonym: PMT9312_0909

Alternate gene names: 78779293

Gene position: 840939-840205 (Counterclockwise)

Preceding gene: 78779295

Following gene: 78779291

Centisome position: 49.2

GC content: 27.89

Gene sequence:

>735_bases
ATGTTTTTGAATAAAAACAATTACAGTAGATTGTTTTTTTTATTTCATATGAATTTATCATTGGATAAAATAAAAATTTT
GGATAAATCATTTAATTACATAATTTCGCCTGAGATATCTAAATTTAGAAGATTTTCACCAAAATTAAAAATTGGAGTAC
TTGCTTCTGGGAAGGGAACAAACTTTCAGGAGTTAATTAATCTCTCAGGAAAAGGAGAATTAGACCTAGAAATAAAAATT
CTAATTACAAACAAAGAAGATGCTGGTTGTATAAAAAGAGCGGTAAAAGCTGAAATACCTCATAAAATAATAAGAAGTGA
AGACTTTTCTCATAAAGAACTATTTGAATTAGAAATTATAAATACATTAATTAATCATGATGTTGAACTTGTAGTAATGG
CTGGCTGGATGAAAATTGTCACTCCATTCTTTATTAACAAATTTAAGAATAAAATTATAAACATTCACCCATCATTACTT
CCTGCATATAAAGGAGGTTCTGCAATAAAGGATTCGATATTAAATGGTTCAAAAATAACTGGTTGCTCAGTACATTTTGT
TGAGGAGGAAGTGGATAGCGGTTCTCTTATAATGCAAGCTGCTTTATCAATCCAACACGACGACAATATTGAAACACTAT
CTAAAAAAATACAAATACTTGAACATAAAATTTTACCTCAATCAATCTCTCATGCTGGTTTTTTGATAAGAAGTAATTTT
ATGGAAAGTTATTAG

Upstream 100 bases:

>100_bases
TTATATATTTTTCCTTTATTTATGATTTAATTATATCTTTACGAATAGTACATAGAATTAATTCTTTTAAATTTGTTTAA
ATTTATATCTTTAAATTTAT

Downstream 100 bases:

>100_bases
TTAACTCAAGGCCATCATCCATTGAAAATCCACTCATAATATTTAAATTTTGAATTGCCTGCCCAGTCTGTCCTTTTAAC
AAATTGTCAATTACAGATAA

Product: phosphoribosylglycinamide formyltransferase

Products: NA

Alternate protein names: 5'-phosphoribosylglycinamide transformylase; GAR transformylase; GART [H]

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MFLNKNNYSRLFFLFHMNLSLDKIKILDKSFNYIISPEISKFRRFSPKLKIGVLASGKGTNFQELINLSGKGELDLEIKI
LITNKEDAGCIKRAVKAEIPHKIIRSEDFSHKELFELEIINTLINHDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLL
PAYKGGSAIKDSILNGSKITGCSVHFVEEEVDSGSLIMQAALSIQHDDNIETLSKKIQILEHKILPQSISHAGFLIRSNF
MESY

Sequences:

>Translated_244_residues
MFLNKNNYSRLFFLFHMNLSLDKIKILDKSFNYIISPEISKFRRFSPKLKIGVLASGKGTNFQELINLSGKGELDLEIKI
LITNKEDAGCIKRAVKAEIPHKIIRSEDFSHKELFELEIINTLINHDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLL
PAYKGGSAIKDSILNGSKITGCSVHFVEEEVDSGSLIMQAALSIQHDDNIETLSKKIQILEHKILPQSISHAGFLIRSNF
MESY
>Mature_244_residues
MFLNKNNYSRLFFLFHMNLSLDKIKILDKSFNYIISPEISKFRRFSPKLKIGVLASGKGTNFQELINLSGKGELDLEIKI
LITNKEDAGCIKRAVKAEIPHKIIRSEDFSHKELFELEIINTLINHDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLL
PAYKGGSAIKDSILNGSKITGCSVHFVEEEVDSGSLIMQAALSIQHDDNIETLSKKIQILEHKILPQSISHAGFLIRSNF
MESY

Specific function: De novo purine biosynthesis; third step. [C]

COG id: COG0299

COG function: function code F; Folate-dependent phosphoribosylglycinamide formyltransferase PurN

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GART family [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=182, Percent_Identity=36.2637362637363, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI209869995, Length=182, Percent_Identity=36.2637362637363, Blast_Score=145, Evalue=3e-35,
Organism=Homo sapiens, GI209869993, Length=182, Percent_Identity=36.2637362637363, Blast_Score=145, Evalue=3e-35,
Organism=Escherichia coli, GI1788846, Length=182, Percent_Identity=33.5164835164835, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1787483, Length=195, Percent_Identity=27.6923076923077, Blast_Score=92, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=189, Percent_Identity=37.037037037037, Blast_Score=146, Evalue=7e-36,
Organism=Saccharomyces cerevisiae, GI6320616, Length=196, Percent_Identity=28.5714285714286, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24582400, Length=184, Percent_Identity=35.8695652173913, Blast_Score=139, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002376
- InterPro:   IPR001555
- InterPro:   IPR004607 [H]

Pfam domain/function: PF00551 Formyl_trans_N [H]

EC number: =2.1.2.2 [H]

Molecular weight: Translated: 27704; Mature: 27704

Theoretical pI: Translated: 9.34; Mature: 9.34

Prosite motif: PS00373 GART

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLNKNNYSRLFFLFHMNLSLDKIKILDKSFNYIISPEISKFRRFSPKLKIGVLASGKGT
CCCCCCCCCEEEEEEEECCCHHHEEEEECCCCEEECCCHHHHHHCCCCEEEEEEECCCCC
NFQELINLSGKGELDLEIKILITNKEDAGCIKRAVKAEIPHKIIRSEDFSHKELFELEII
CHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
NTLINHDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLLPAYKGGSAIKDSILNGSKIT
HHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCCCCC
GCSVHFVEEEVDSGSLIMQAALSIQHDDNIETLSKKIQILEHKILPQSISHAGFLIRSNF
CEEEEEECCCCCCCHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
MESY
HHCC
>Mature Secondary Structure
MFLNKNNYSRLFFLFHMNLSLDKIKILDKSFNYIISPEISKFRRFSPKLKIGVLASGKGT
CCCCCCCCCEEEEEEEECCCHHHEEEEECCCCEEECCCHHHHHHCCCCEEEEEEECCCCC
NFQELINLSGKGELDLEIKILITNKEDAGCIKRAVKAEIPHKIIRSEDFSHKELFELEII
CHHHHHCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHCCHHHHCCCCCCHHHHHHHHHH
NTLINHDVELVVMAGWMKIVTPFFINKFKNKIINIHPSLLPAYKGGSAIKDSILNGSKIT
HHHHCCCEEEEEEHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCHHHHHHCCCCCCC
GCSVHFVEEEVDSGSLIMQAALSIQHDDNIETLSKKIQILEHKILPQSISHAGFLIRSNF
CEEEEEECCCCCCCHHEEHHHHHCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHH
MESY
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]