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The map label for this gene is pyk2 [H]

Identifier: 78779272

GI number: 78779272

Start: 820354

End: 822147

Strand: Reverse

Name: pyk2 [H]

Synonym: PMT9312_0888

Alternate gene names: 78779272

Gene position: 822147-820354 (Counterclockwise)

Preceding gene: 78779274

Following gene: 78779271

Centisome position: 48.1

GC content: 33.22

Gene sequence:

>1794_bases
ATGTCGAATATTGATTTAAAAAGAAGAACAAAAATAGTAGCAACTATTGGACCTGCAACTCAGTCTGAAGAGATAATTAC
AAATTTAATTAAAGCAGGAGTAACAACATTCAGATTAAATTTCTCACATGGAGATCATAATGATCACGCTGAGAGAATTA
AAACTATAAGGGAGGTTTCACAAAAGCTAGATATTGATATTGGTATTCTGCAAGATCTTCAAGGACCTAAAATTAGGTTA
GGACGATTCAAGGATGGTCCAGTAAAAGTAAAAAAAGGGGATAAATTTACACTAACCTCCAATGAAGTTGAATGTTCAAA
TACTATTGCGAATGTAACTTACGAGAATCTTTCTCAAGAAGTTAGTGAAGGGAAAAGAATACTATTAGATGATGGTAAAA
TTGAAATGATTGTTGAAAAAGTAGATACTAAAGCTAATCTTTTAGAATGTTTGGTTACTGTAGGTGGTGTTTTATCAAAT
AATAAAGGTGTAAATTTCCCAGATGTTCAATTATCTGTAAAAGCATTAACAGAAAAAGATAAAGAGGATTTAAAATTTGG
TTTATCTGAGGGAGTTGACTGGATAGCTCTGAGTTTCGTAAGAAATCCTTCCGATATTAATGAAATTAAAGATTTAATAA
ACAAAAATGGTCATTCAACTCCAGTAGTTGCAAAAATAGAAAAATTTGAAGCAATAGATCAAATTGATGCTGTATTACCT
TTATGTGATGGAGTTATGGTTGCAAGAGGGGATTTGGGAGTAGAAATGCCTGCTGAAGAAGTTCCACTTTTACAAAAGGA
TTTAATAAGAAAAGCTAATTCATTAGGTATACCAATAATTACCGCGACTCAAATGCTTGATTCCATGGCTTCTAATCCAA
GACCGACTAGAGCCGAAGTAAGCGATGTCGCCAATGCAATACTGGATGGAACAGATGCAGTAATGCTTTCAAACGAAACT
GCCGTTGGCGATTATCCTGTAGAGGCAGTACAAACGATGGCAACAATAGCAAGAAGAATTGAGAGGGATTATCCTCTTAA
AGCTATTGAGAGCCACTTACCTAGCACGATACCAAATGCTATTAGTGGAGCTGTAAGTAATATAGCTAGACAACTAGATG
CAGGAGCAATAATTCCTTTAACAAAATCAGGTTCTACTGCTCGAAATGTAAGTAAATTTAGACCGCCAACTCCTATTTTG
GCAACTACAACTGAAAGGAGTGTAGCGAGAAGACTTCAACTTGTTTGGGGAGTCACCCCGATAGTAGTAAATAATGATGA
AAGAACAGCAAAAACATTTAGTTTAGCTATGCAAATTGCTCAAGAAATGGGAATATTAAAACAAGGAGATTTAGTCGTTC
AAACTGCAGGTACACTAACTGGTATAAGTGGCTCTACAGATTTAATAAAAGTTGGTTTAGTAAGGAAGATACTTTCAAGA
GGGATCTCAATAGGTGAAATTGGAGTTACAGGTAAAGCAAGAAAAATAAATACTAAACTTGATTTGTCATTAATTTGCCC
TGGCGAAATTTTATTTGTTAATAAAGAATTATTGGAAAATGTTCCATTGAGCAAAAATATTGCAGGTATTGTTACGAACC
AAAATGTAGATGATGTTTATGCCTTTTTTAACAAAAACAATAAGAAGATTTCTACAATCTGTAATGTAGAAAACATTGAT
AATAATCAAATTATTAATGGTGATCTAGTAACTTTACAGCTTAACGAAGGTGTAATTTATATGGGACAAATTGAAGATGA
TGAAGAAGCTATAGATAAATATAAATATGTCTAG

Upstream 100 bases:

>100_bases
TGTTTCTCTTGCTTTTTTCTGATAAGTTTTAAAATCCATAACTTAAACAACTAACTTTGGGTATGGTAAATTTATTTATA
TCTAGCAATATTATTTATAT

Downstream 100 bases:

>100_bases
GAATATCTCAATAAAGGAAGCCCTAGGCATGGCGACCAAAACATTAGTCTCAAACAAATTAAGAAGTTCGTTGACTATGC
TAGGAATAATTATAGGAAAT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 2 [H]

Number of amino acids: Translated: 597; Mature: 596

Protein sequence:

>597_residues
MSNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHNDHAERIKTIREVSQKLDIDIGILQDLQGPKIRL
GRFKDGPVKVKKGDKFTLTSNEVECSNTIANVTYENLSQEVSEGKRILLDDGKIEMIVEKVDTKANLLECLVTVGGVLSN
NKGVNFPDVQLSVKALTEKDKEDLKFGLSEGVDWIALSFVRNPSDINEIKDLINKNGHSTPVVAKIEKFEAIDQIDAVLP
LCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPVEAVQTMATIARRIERDYPLKAIESHLPSTIPNAISGAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPIL
ATTTERSVARRLQLVWGVTPIVVNNDERTAKTFSLAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKILSR
GISIGEIGVTGKARKINTKLDLSLICPGEILFVNKELLENVPLSKNIAGIVTNQNVDDVYAFFNKNNKKISTICNVENID
NNQIINGDLVTLQLNEGVIYMGQIEDDEEAIDKYKYV

Sequences:

>Translated_597_residues
MSNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHNDHAERIKTIREVSQKLDIDIGILQDLQGPKIRL
GRFKDGPVKVKKGDKFTLTSNEVECSNTIANVTYENLSQEVSEGKRILLDDGKIEMIVEKVDTKANLLECLVTVGGVLSN
NKGVNFPDVQLSVKALTEKDKEDLKFGLSEGVDWIALSFVRNPSDINEIKDLINKNGHSTPVVAKIEKFEAIDQIDAVLP
LCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNET
AVGDYPVEAVQTMATIARRIERDYPLKAIESHLPSTIPNAISGAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPIL
ATTTERSVARRLQLVWGVTPIVVNNDERTAKTFSLAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKILSR
GISIGEIGVTGKARKINTKLDLSLICPGEILFVNKELLENVPLSKNIAGIVTNQNVDDVYAFFNKNNKKISTICNVENID
NNQIINGDLVTLQLNEGVIYMGQIEDDEEAIDKYKYV
>Mature_596_residues
SNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHNDHAERIKTIREVSQKLDIDIGILQDLQGPKIRLG
RFKDGPVKVKKGDKFTLTSNEVECSNTIANVTYENLSQEVSEGKRILLDDGKIEMIVEKVDTKANLLECLVTVGGVLSNN
KGVNFPDVQLSVKALTEKDKEDLKFGLSEGVDWIALSFVRNPSDINEIKDLINKNGHSTPVVAKIEKFEAIDQIDAVLPL
CDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASNPRPTRAEVSDVANAILDGTDAVMLSNETA
VGDYPVEAVQTMATIARRIERDYPLKAIESHLPSTIPNAISGAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILA
TTTERSVARRLQLVWGVTPIVVNNDERTAKTFSLAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKILSRG
ISIGEIGVTGKARKINTKLDLSLICPGEILFVNKELLENVPLSKNIAGIVTNQNVDDVYAFFNKNNKKISTICNVENIDN
NQIINGDLVTLQLNEGVIYMGQIEDDEEAIDKYKYV

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=39.9590163934426, Blast_Score=334, Evalue=1e-91,
Organism=Homo sapiens, GI33286422, Length=489, Percent_Identity=39.8773006134969, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI33286420, Length=489, Percent_Identity=39.8773006134969, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI10835121, Length=490, Percent_Identity=39.5918367346939, Blast_Score=325, Evalue=6e-89,
Organism=Homo sapiens, GI32967597, Length=490, Percent_Identity=39.5918367346939, Blast_Score=325, Evalue=9e-89,
Organism=Homo sapiens, GI310128732, Length=289, Percent_Identity=40.1384083044983, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI310128730, Length=289, Percent_Identity=40.1384083044983, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI310128736, Length=237, Percent_Identity=37.1308016877637, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI310128734, Length=237, Percent_Identity=37.1308016877637, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI310128738, Length=216, Percent_Identity=36.1111111111111, Blast_Score=131, Evalue=2e-30,
Organism=Escherichia coli, GI1787965, Length=469, Percent_Identity=43.7100213219616, Blast_Score=357, Evalue=2e-99,
Organism=Escherichia coli, GI1788160, Length=473, Percent_Identity=42.2832980972516, Blast_Score=322, Evalue=5e-89,
Organism=Caenorhabditis elegans, GI17544584, Length=485, Percent_Identity=38.5567010309278, Blast_Score=325, Evalue=6e-89,
Organism=Caenorhabditis elegans, GI71984413, Length=487, Percent_Identity=40.2464065708419, Blast_Score=304, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI17506829, Length=487, Percent_Identity=40.2464065708419, Blast_Score=304, Evalue=9e-83,
Organism=Caenorhabditis elegans, GI71984406, Length=487, Percent_Identity=40.2464065708419, Blast_Score=303, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI17506831, Length=487, Percent_Identity=40.2464065708419, Blast_Score=303, Evalue=1e-82,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=39.3004115226337, Blast_Score=304, Evalue=3e-83,
Organism=Saccharomyces cerevisiae, GI6324923, Length=478, Percent_Identity=38.4937238493724, Blast_Score=295, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24648964, Length=491, Percent_Identity=39.5112016293279, Blast_Score=316, Evalue=3e-86,
Organism=Drosophila melanogaster, GI28571814, Length=491, Percent_Identity=39.5112016293279, Blast_Score=315, Evalue=5e-86,
Organism=Drosophila melanogaster, GI24648966, Length=408, Percent_Identity=40.4411764705882, Blast_Score=286, Evalue=2e-77,
Organism=Drosophila melanogaster, GI24581235, Length=486, Percent_Identity=33.3333333333333, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24646914, Length=329, Percent_Identity=37.6899696048632, Blast_Score=190, Evalue=3e-48,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 65036; Mature: 64904

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHNDHAERIKTIREVS
CCCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHH
QKLDIDIGILQDLQGPKIRLGRFKDGPVKVKKGDKFTLTSNEVECSNTIANVTYENLSQE
HHCCCCEEHHHCCCCCCEEECCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHH
VSEGKRILLDDGKIEMIVEKVDTKANLLECLVTVGGVLSNNKGVNFPDVQLSVKALTEKD
HHCCCEEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHCCC
KEDLKFGLSEGVDWIALSFVRNPSDINEIKDLINKNGHSTPVVAKIEKFEAIDQIDAVLP
HHHHHHHHHCCCCEEEHEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASNPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPLKAIESHLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHH
ISGAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILATTTERSVARRLQLVWGVTP
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCE
IVVNNDERTAKTFSLAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKILSR
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHC
GISIGEIGVTGKARKINTKLDLSLICPGEILFVNKELLENVPLSKNIAGIVTNQNVDDVY
CCCEEEECCCCCCEEECCEEEEEEECCCEEEEEEHHHHHCCCCCCCCEEEEECCCHHHHH
AFFNKNNKKISTICNVENIDNNQIINGDLVTLQLNEGVIYMGQIEDDEEAIDKYKYV
HHHCCCCCEEEEEECCCCCCCCEEECCEEEEEEECCCEEEEECCCCHHHHHHHHCCC
>Mature Secondary Structure 
SNIDLKRRTKIVATIGPATQSEEIITNLIKAGVTTFRLNFSHGDHNDHAERIKTIREVS
CCCCCCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHHHH
QKLDIDIGILQDLQGPKIRLGRFKDGPVKVKKGDKFTLTSNEVECSNTIANVTYENLSQE
HHCCCCEEHHHCCCCCCEEECCCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHH
VSEGKRILLDDGKIEMIVEKVDTKANLLECLVTVGGVLSNNKGVNFPDVQLSVKALTEKD
HHCCCEEEEECCCEEEEEEHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEHHHHHCCC
KEDLKFGLSEGVDWIALSFVRNPSDINEIKDLINKNGHSTPVVAKIEKFEAIDQIDAVLP
HHHHHHHHHCCCCEEEHEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
LCDGVMVARGDLGVEMPAEEVPLLQKDLIRKANSLGIPIITATQMLDSMASNPRPTRAEV
HHCCEEEEECCCCCCCCHHHCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHCCCCCCHHHH
SDVANAILDGTDAVMLSNETAVGDYPVEAVQTMATIARRIERDYPLKAIESHLPSTIPNA
HHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHH
ISGAVSNIARQLDAGAIIPLTKSGSTARNVSKFRPPTPILATTTERSVARRLQLVWGVTP
HHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCEEEECCHHHHHHHHHHHHCCCE
IVVNNDERTAKTFSLAMQIAQEMGILKQGDLVVQTAGTLTGISGSTDLIKVGLVRKILSR
EEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCEECCCCCHHHHHHHHHHHHHHC
GISIGEIGVTGKARKINTKLDLSLICPGEILFVNKELLENVPLSKNIAGIVTNQNVDDVY
CCCEEEECCCCCCEEECCEEEEEEECCCEEEEEEHHHHHCCCCCCCCEEEEECCCHHHHH
AFFNKNNKKISTICNVENIDNNQIINGDLVTLQLNEGVIYMGQIEDDEEAIDKYKYV
HHHCCCCCEEEEEECCCCCCCCEEECCEEEEEEECCCEEEEECCCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]