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The map label for this gene is ruvA

Identifier: 78779241

GI number: 78779241

Start: 793472

End: 794149

Strand: Reverse

Name: ruvA

Synonym: PMT9312_0857

Alternate gene names: 78779241

Gene position: 794149-793472 (Counterclockwise)

Preceding gene: 78779242

Following gene: 78779240

Centisome position: 46.46

GC content: 24.19

Gene sequence:

>678_bases
TTGATTAGTTGGATTAGTGGAGAATTGGTTGAATTATGGCAAACTAATCAAAAATTTTTTCTTTTAATAAATTGTCAAGG
ATTAGGGTACGAAATTCAAGTCTTAGAATCTTTATTTCTCAAATTAAAAACGAATCAGATAACTAATAAAAACATCACTC
TTTGGATAAAACATATTAAAAAAGAAGATTCAGATTTATTATTTGGATTTAGCTCGAAGGATCAAAAAAATTTTTTTATT
GAAATTTTAAATATTAGAGGTGTTGGTTCCCAAATTGGTATGGGTATATTAAGTAAATTTTCCATTAGTGAAGTAATCAA
TGCAATTAATACACAAAACAAAAAATTAATTTGTTCCGTACCTGGTATAGGACAAAAAATGAGTGAGAGGTTAATTTTAG
AATTAAAAAGTAAATTTAGAAACGAATTAAAAATTCAGGAAGAAAAAAGCAAAGATGAATTTCATATTAAGGATAATAAA
ATTAATAAAATTGTGAGTGACATTGAATTAACCTTAAAGTCCTTAAACTACACTAAGAATGAAATAAAAAGTATTTTGCC
AATCATTAGCAAAGAAATAGATAGCCTTACTAAAAAGGAAAAAGATACATCATTTGAAAATTTATTAATGTTAGCTATGA
ATTATTTAGATAATGATAGTAGTAATATAGTCAGATAA

Upstream 100 bases:

>100_bases
AATAGCACTTTCCTCCTCTAGAGGGAAAGATAGATTTTGGGCAGTACCTGCTGGAGGAACTGCAGGAGCACTAATTGGAT
GTCAGGTGGATGGTGGTTAA

Downstream 100 bases:

>100_bases
CGTAGTAATATAGATTTAAGAAAATTAATTTTATGTCATTAGATACGGCCGAAAAACAGAAACTTATTGAAACCCATCAA
GTTCATGCAACTGATACTGG

Product: Holliday junction DNA helicase subunit RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MISWISGELVELWQTNQKFFLLINCQGLGYEIQVLESLFLKLKTNQITNKNITLWIKHIKKEDSDLLFGFSSKDQKNFFI
EILNIRGVGSQIGMGILSKFSISEVINAINTQNKKLICSVPGIGQKMSERLILELKSKFRNELKIQEEKSKDEFHIKDNK
INKIVSDIELTLKSLNYTKNEIKSILPIISKEIDSLTKKEKDTSFENLLMLAMNYLDNDSSNIVR

Sequences:

>Translated_225_residues
MISWISGELVELWQTNQKFFLLINCQGLGYEIQVLESLFLKLKTNQITNKNITLWIKHIKKEDSDLLFGFSSKDQKNFFI
EILNIRGVGSQIGMGILSKFSISEVINAINTQNKKLICSVPGIGQKMSERLILELKSKFRNELKIQEEKSKDEFHIKDNK
INKIVSDIELTLKSLNYTKNEIKSILPIISKEIDSLTKKEKDTSFENLLMLAMNYLDNDSSNIVR
>Mature_225_residues
MISWISGELVELWQTNQKFFLLINCQGLGYEIQVLESLFLKLKTNQITNKNITLWIKHIKKEDSDLLFGFSSKDQKNFFI
EILNIRGVGSQIGMGILSKFSISEVINAINTQNKKLICSVPGIGQKMSERLILELKSKFRNELKIQEEKSKDEFHIKDNK
INKIVSDIELTLKSLNYTKNEIKSILPIISKEIDSLTKKEKDTSFENLLMLAMNYLDNDSSNIVR

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=141, Percent_Identity=30.4964539007092, Blast_Score=90, Evalue=1e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_PROM9 (Q31B28)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397353.1
- ProteinModelPortal:   Q31B28
- SMR:   Q31B28
- STRING:   Q31B28
- GeneID:   3765658
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_0857
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   IATKEVK
- ProtClustDB:   CLSK922035
- BioCyc:   PMAR74546:PMT9312_0857-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278

Pfam domain/function: PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =3.6.4.12

Molecular weight: Translated: 25950; Mature: 25950

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MISWISGELVELWQTNQKFFLLINCQGLGYEIQVLESLFLKLKTNQITNKNITLWIKHIK
CCCCCCHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
KEDSDLLFGFSSKDQKNFFIEILNIRGVGSQIGMGILSKFSISEVINAINTQNKKLICSV
CCCCCEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PGIGQKMSERLILELKSKFRNELKIQEEKSKDEFHIKDNKINKIVSDIELTLKSLNYTKN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCHH
EIKSILPIISKEIDSLTKKEKDTSFENLLMLAMNYLDNDSSNIVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MISWISGELVELWQTNQKFFLLINCQGLGYEIQVLESLFLKLKTNQITNKNITLWIKHIK
CCCCCCHHHHHHHCCCCEEEEEEEECCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
KEDSDLLFGFSSKDQKNFFIEILNIRGVGSQIGMGILSKFSISEVINAINTQNKKLICSV
CCCCCEEEEECCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEC
PGIGQKMSERLILELKSKFRNELKIQEEKSKDEFHIKDNKINKIVSDIELTLKSLNYTKN
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCHHHHHHHHHHHHHHHHCCCCHH
EIKSILPIISKEIDSLTKKEKDTSFENLLMLAMNYLDNDSSNIVR
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA