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The map label for this gene is gatA

Identifier: 78779237

GI number: 78779237

Start: 787678

End: 789120

Strand: Direct

Name: gatA

Synonym: PMT9312_0853

Alternate gene names: 78779237

Gene position: 787678-789120 (Clockwise)

Preceding gene: 78779236

Following gene: 78779238

Centisome position: 46.08

GC content: 34.51

Gene sequence:

>1443_bases
ATGGATTTTAATTCTCTCAGAAAGAAAATTATTACTAAAAAAGCTTCCGTTAAGGAATTAGTAAATGATATTTTTTTAAA
AATCGATTCTGATGATCCTGAAATTAATTCATATATTTGTACTACAAAAGATAATGCAATCCAGCAAGCTGAAAATATAG
ATAAATTAATTCAAAATGAAGAAATACTTCCTCCTCTTGCAGGAATGCCAATAGCAATTAAAGATAATATTTGCACTAAG
GGTGTCCATACCACTTGTGCGAGTAAAATGCTCAAAGGTTTTATCGCACCGTATGAGTCCACAGCTTCAGATAAATTATG
GTCCTCAGGTGGCATTTGTTTAGGCAAAACAAATTTGGATGAATTTGCAATGGGCAGTTCAACAGAAACTTCTGTATTTG
GCGTTACTTCAAACCCTTGGGATATTAGTAGAGTTCCAGGAGGAAGTTCTGGAGGCAGTGCCGCTTCTGTTGCTGCTGGA
TTATGTTCTGCAGCTATAGGTTCTGATACTGGAGGATCAATAAGACAACCAGCTTCATTTTGTGGTGTTGTAGGACTTAA
GCCTACCTATGGAAGAGTAAGTAGATGGGGACTAGTAGCATTTGCTAGTTCTCTTGATCAAATTGGTCCAATTACAAATA
CTGTCTCAGATGCCGCTGAAATTCTTTATTCAATATCTGGTAAAGATCCCTTAGATTCAACATGTCTTGATCAACCAGTA
CCAAATTATTTGATTAATTTAAATAAGTCTATAAAGGATTTAAAAATTGGAATCATTAAAGAATGCTTTGAACATCCTGG
TCTTAATACCGAAGTTAAAGATTCTGTTCTTTGTGGAGTAGAAAGATTCAAAAACTTAGGAGCCGAAATTATTGAAGTTG
AATGTCCTAGGTTCAACGACGGAATTGCCACATATTATGTAATTGCCCCTTCTGAAGCATCTGCAAATTTAGCTAGATAC
GATGGAGTTAAATATGGTTTCAGATCAGAAGAAGGTTCTAATCTTATAGATATGACTTCAAAAAGTAGAGCTGAAGGATT
TGGAGATGAAGTTCAGAGAAGAATTTTGATAGGAACTTATGCTTTGTCCGCAGGATACAGTGACGCATACTATAAGAAAG
CACAAAAAGTTAGAACATTAATAAGAAATGATTTTGATAAAGCTTTTAAAAAAGTAGATATTTTATTAACTCCGACTTGT
CCAACTACCGCTTTTTTAAAAGGTGATTTTAGCAACGATCCTCTTTCCATGTATTTGTCTGACCTCTTAACTGTTCCCGC
AAATTTAGCAGGACTCCCAGCTATCAGTATCCCTTGTGGTTTTGATACTAAAGGATTACCTATAGGACTTCAATTAATAG
GTAATGTATTGGAAGAGGATAAAATATTGAATGCAGCACATATTTTTGAAATTGATGCTCAAGTAATTAAGAATAGACTT
TGA

Upstream 100 bases:

>100_bases
ATGAAGGTTTTGCTAAAATTGAACATAGTTTAATTCGTTGTAAAAAAGAAGAGCCATTAACTGTTTGATATTCTTAAAAT
TTTAATAAATAAGATGAGAA

Downstream 100 bases:

>100_bases
TTCTAAATTTGTATTAATAATTAATTTGTAATCACAAAATATAGACCTTTTAAAAATTTTCTCTATCTTATATATATAAA
TAGATTGAGTATGGGTTTCA

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 480; Mature: 480

Protein sequence:

>480_residues
MDFNSLRKKIITKKASVKELVNDIFLKIDSDDPEINSYICTTKDNAIQQAENIDKLIQNEEILPPLAGMPIAIKDNICTK
GVHTTCASKMLKGFIAPYESTASDKLWSSGGICLGKTNLDEFAMGSSTETSVFGVTSNPWDISRVPGGSSGGSAASVAAG
LCSAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPLDSTCLDQPV
PNYLINLNKSIKDLKIGIIKECFEHPGLNTEVKDSVLCGVERFKNLGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGFRSEEGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRNDFDKAFKKVDILLTPTC
PTTAFLKGDFSNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDKILNAAHIFEIDAQVIKNRL

Sequences:

>Translated_480_residues
MDFNSLRKKIITKKASVKELVNDIFLKIDSDDPEINSYICTTKDNAIQQAENIDKLIQNEEILPPLAGMPIAIKDNICTK
GVHTTCASKMLKGFIAPYESTASDKLWSSGGICLGKTNLDEFAMGSSTETSVFGVTSNPWDISRVPGGSSGGSAASVAAG
LCSAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPLDSTCLDQPV
PNYLINLNKSIKDLKIGIIKECFEHPGLNTEVKDSVLCGVERFKNLGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGFRSEEGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRNDFDKAFKKVDILLTPTC
PTTAFLKGDFSNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDKILNAAHIFEIDAQVIKNRL
>Mature_480_residues
MDFNSLRKKIITKKASVKELVNDIFLKIDSDDPEINSYICTTKDNAIQQAENIDKLIQNEEILPPLAGMPIAIKDNICTK
GVHTTCASKMLKGFIAPYESTASDKLWSSGGICLGKTNLDEFAMGSSTETSVFGVTSNPWDISRVPGGSSGGSAASVAAG
LCSAAIGSDTGGSIRQPASFCGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPLDSTCLDQPV
PNYLINLNKSIKDLKIGIIKECFEHPGLNTEVKDSVLCGVERFKNLGAEIIEVECPRFNDGIATYYVIAPSEASANLARY
DGVKYGFRSEEGSNLIDMTSKSRAEGFGDEVQRRILIGTYALSAGYSDAYYKKAQKVRTLIRNDFDKAFKKVDILLTPTC
PTTAFLKGDFSNDPLSMYLSDLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDKILNAAHIFEIDAQVIKNRL

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=468, Percent_Identity=43.3760683760684, Blast_Score=329, Evalue=3e-90,
Organism=Homo sapiens, GI195972892, Length=496, Percent_Identity=25.4032258064516, Blast_Score=122, Evalue=6e-28,
Organism=Homo sapiens, GI166795287, Length=133, Percent_Identity=38.3458646616541, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17543272, Length=448, Percent_Identity=37.9464285714286, Blast_Score=266, Evalue=1e-71,
Organism=Caenorhabditis elegans, GI17537465, Length=485, Percent_Identity=26.8041237113402, Blast_Score=132, Evalue=5e-31,
Organism=Caenorhabditis elegans, GI71990152, Length=284, Percent_Identity=32.0422535211268, Blast_Score=114, Evalue=1e-25,
Organism=Caenorhabditis elegans, GI17556264, Length=235, Percent_Identity=33.6170212765957, Blast_Score=108, Evalue=7e-24,
Organism=Caenorhabditis elegans, GI17538252, Length=322, Percent_Identity=27.3291925465839, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17556276, Length=199, Percent_Identity=33.1658291457286, Blast_Score=75, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17556278, Length=199, Percent_Identity=33.1658291457286, Blast_Score=75, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17538254, Length=270, Percent_Identity=28.1481481481481, Blast_Score=75, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6323950, Length=423, Percent_Identity=36.8794326241135, Blast_Score=242, Evalue=9e-65,
Organism=Saccharomyces cerevisiae, GI6319685, Length=443, Percent_Identity=31.8284424379233, Blast_Score=129, Evalue=9e-31,
Organism=Saccharomyces cerevisiae, GI6320448, Length=134, Percent_Identity=39.5522388059701, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI24648113, Length=457, Percent_Identity=43.7636761487965, Blast_Score=329, Evalue=3e-90,
Organism=Drosophila melanogaster, GI24644968, Length=478, Percent_Identity=26.1506276150628, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24652985, Length=491, Percent_Identity=24.847250509165, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922090, Length=491, Percent_Identity=24.847250509165, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652981, Length=491, Percent_Identity=24.847250509165, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI24652983, Length=491, Percent_Identity=24.847250509165, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI45550774, Length=476, Percent_Identity=26.2605042016807, Blast_Score=125, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24648435, Length=476, Percent_Identity=26.2605042016807, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24648437, Length=476, Percent_Identity=26.2605042016807, Blast_Score=125, Evalue=7e-29,
Organism=Drosophila melanogaster, GI161078093, Length=483, Percent_Identity=27.536231884058, Blast_Score=124, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24648441, Length=433, Percent_Identity=26.0969976905312, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI24648439, Length=433, Percent_Identity=26.0969976905312, Blast_Score=109, Evalue=5e-24,
Organism=Drosophila melanogaster, GI21356731, Length=473, Percent_Identity=24.1014799154334, Blast_Score=100, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51649; Mature: 51649

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFNSLRKKIITKKASVKELVNDIFLKIDSDDPEINSYICTTKDNAIQQAENIDKLIQNE
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCC
EILPPLAGMPIAIKDNICTKGVHTTCASKMLKGFIAPYESTASDKLWSSGGICLGKTNLD
CCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEECCCCH
EFAMGSSTETSVFGVTSNPWDISRVPGGSSGGSAASVAAGLCSAAIGSDTGGSIRQPASF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPLDSTCLDQPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCC
PNYLINLNKSIKDLKIGIIKECFEHPGLNTEVKDSVLCGVERFKNLGAEIIEVECPRFND
CHHHEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGFRSEEGSNLIDMTSKSRAEGFGDEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHEEEEE
ALSAGYSDAYYKKAQKVRTLIRNDFDKAFKKVDILLTPTCPTTAFLKGDFSNDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDKILNAAHIFEIDAQVIKNRL
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDFNSLRKKIITKKASVKELVNDIFLKIDSDDPEINSYICTTKDNAIQQAENIDKLIQNE
CCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCCCEEEECCCHHHHHHHHHHHHHCCC
EILPPLAGMPIAIKDNICTKGVHTTCASKMLKGFIAPYESTASDKLWSSGGICLGKTNLD
CCCCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHCCCCCEEEECCCCH
EFAMGSSTETSVFGVTSNPWDISRVPGGSSGGSAASVAAGLCSAAIGSDTGGSIRQPASF
HHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHH
CGVVGLKPTYGRVSRWGLVAFASSLDQIGPITNTVSDAAEILYSISGKDPLDSTCLDQPV
HCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCHHHCCCCC
PNYLINLNKSIKDLKIGIIKECFEHPGLNTEVKDSVLCGVERFKNLGAEIIEVECPRFND
CHHHEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCEEEEEECCCCCC
GIATYYVIAPSEASANLARYDGVKYGFRSEEGSNLIDMTSKSRAEGFGDEVQRRILIGTY
CCEEEEEEECCCCCCCHHHHCCCCCCCCCCCCCCEEECCCCHHHCCCCHHHHHHHEEEEE
ALSAGYSDAYYKKAQKVRTLIRNDFDKAFKKVDILLTPTCPTTAFLKGDFSNDPLSMYLS
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCEEEECCCCCCHHHHHHH
DLLTVPANLAGLPAISIPCGFDTKGLPIGLQLIGNVLEEDKILNAAHIFEIDAQVIKNRL
HHHHCCCCCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA