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The map label for this gene is hemK [C]

Identifier: 78778709

GI number: 78778709

Start: 307947

End: 308816

Strand: Direct

Name: hemK [C]

Synonym: PMT9312_0324

Alternate gene names: 78778709

Gene position: 307947-308816 (Clockwise)

Preceding gene: 78778708

Following gene: 78778710

Centisome position: 18.02

GC content: 29.31

Gene sequence:

>870_bases
ATGCTTAGCATTTCAGTAAAAGAATTTTTAATTTGGAAAAAACTACAACTTTCTAAAGGAGGAGATCAAGAATCTTTTGC
TTTTTTACTTGACTGTATAGGCGGAATTTCGAATTTTGATTTAAATTTGCTGAGCATAAATCCAAAGGGAAACCTATATT
TAAAAAAAAAATTAGACTTTTTAGAATCTGTTTGGGACGATCATTTAGTAAAATGTTACCCAATTCAATACCTTTGTGAA
ACAACTTATTGGAGAGATTTAAAATTAAAAGTTACAAATAAAGTACTTATCCCTAGGCCAGAAACTGAACTTATAGTTGA
TATTGTTTTTAATATATTTGGAAAGCAATCACGAAAATTATTTTTCGCTGAATTAGGAACTGGATCAGGTGCTATAAGTA
TTGCTTTGGCATTAGCGTATCCATTGTGTAATGGAATAGCAACTGATATAGATCAAGATGCATTAGAAATAGCCATTAAT
AATTATATGAATTCTTCTAAACAATCAAATTTGAAATTTTATTGTGGACATTGGTGGACCCCTCTTGAGAGTTTTAAAGG
AAAATTAGATCTCGCCATTTCAAACCCGCCATATATCCCTAAAGATACTTATGAAAAATTACCCAAAGAAGTTAAAAATT
TCGAACCAAGAATTGCTTTATTAGGCGGCGAAGATGGTTTACAACATATTAGGGAAATAATTCAAAAAGCACCTATTTTT
TTAAAAGAGAAGGGATGGTTAATTTTAGAGAATCATTTTGATCAAGGTGAAAAAGTCAAACAACTTTTTATTAAGAATAA
ATTTACATCAATAGAAATTGTGAATGATCTTTCAGGTATTGGTAGGTTTACCATAGGAAGATATAAATAA

Upstream 100 bases:

>100_bases
TTATTGGTTTATTTATTTCTACCCAAGATGGCGAAAAATCAAGCTTCTTCTCTGACTCTAGTAAAGGTAAGCTTGGCCCA
AAACGCTAAAGCTTTTATAA

Downstream 100 bases:

>100_bases
AGTATTGCGGGTTCATAATTAAAATGAATTTAGTAGACTCTAAATCAGCTTTGAAGACTCTTACTAGTGGTTTACCTATA
ATTTTCCCAACAGATACCTT

Product: HemK family modification methylase

Products: Protoporphyrin IX; H2O

Alternate protein names: M.Ssp6803HemKP [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MLSISVKEFLIWKKLQLSKGGDQESFAFLLDCIGGISNFDLNLLSINPKGNLYLKKKLDFLESVWDDHLVKCYPIQYLCE
TTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFGKQSRKLFFAELGTGSGAISIALALAYPLCNGIATDIDQDALEIAIN
NYMNSSKQSNLKFYCGHWWTPLESFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPRIALLGGEDGLQHIREIIQKAPIF
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVNDLSGIGRFTIGRYK

Sequences:

>Translated_289_residues
MLSISVKEFLIWKKLQLSKGGDQESFAFLLDCIGGISNFDLNLLSINPKGNLYLKKKLDFLESVWDDHLVKCYPIQYLCE
TTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFGKQSRKLFFAELGTGSGAISIALALAYPLCNGIATDIDQDALEIAIN
NYMNSSKQSNLKFYCGHWWTPLESFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPRIALLGGEDGLQHIREIIQKAPIF
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVNDLSGIGRFTIGRYK
>Mature_289_residues
MLSISVKEFLIWKKLQLSKGGDQESFAFLLDCIGGISNFDLNLLSINPKGNLYLKKKLDFLESVWDDHLVKCYPIQYLCE
TTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFGKQSRKLFFAELGTGSGAISIALALAYPLCNGIATDIDQDALEIAIN
NYMNSSKQSNLKFYCGHWWTPLESFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPRIALLGGEDGLQHIREIIQKAPIF
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVNDLSGIGRFTIGRYK

Specific function: Probable protein methyltransferase. May methylate a Gln residue in target proteins [H]

COG id: COG2890

COG function: function code J; Methylase of polypeptide chain release factors

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hemK family [H]

Homologues:

Organism=Homo sapiens, GI7705409, Length=224, Percent_Identity=30.8035714285714, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI1787463, Length=215, Percent_Identity=33.4883720930233, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI87082085, Length=178, Percent_Identity=33.1460674157303, Blast_Score=85, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24582226, Length=196, Percent_Identity=31.1224489795918, Blast_Score=75, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR004556
- InterPro:   IPR019874
- InterPro:   IPR007848 [H]

Pfam domain/function: PF05175 MTS [H]

EC number: 1.3.3.4

Molecular weight: Translated: 33031; Mature: 33031

Theoretical pI: Translated: 8.43; Mature: 8.43

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSISVKEFLIWKKLQLSKGGDQESFAFLLDCIGGISNFDLNLLSINPKGNLYLKKKLDF
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHH
LESVWDDHLVKCYPIQYLCETTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFGKQSRKL
HHHHHHCCCEEEECHHHHHCCCCEEEEEEEEECEEECCCCCHHHHHHHHHHHHCCCCCEE
FFAELGTGSGAISIALALAYPLCNGIATDIDQDALEIAINNYMNSSKQSNLKFYCGHWWT
EEEEECCCCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCC
PLESFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPRIALLGGEDGLQHIREIIQKAPIF
CHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEE
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVNDLSGIGRFTIGRYK
EECCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLSISVKEFLIWKKLQLSKGGDQESFAFLLDCIGGISNFDLNLLSINPKGNLYLKKKLDF
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCCCCEEEHHHHHH
LESVWDDHLVKCYPIQYLCETTYWRDLKLKVTNKVLIPRPETELIVDIVFNIFGKQSRKL
HHHHHHCCCEEEECHHHHHCCCCEEEEEEEEECEEECCCCCHHHHHHHHHHHHCCCCCEE
FFAELGTGSGAISIALALAYPLCNGIATDIDQDALEIAINNYMNSSKQSNLKFYCGHWWT
EEEEECCCCCHHHHHHHHHHHHHCCHHHCCCHHHHHHHHHHHHCCCCCCCCEEEECCCCC
PLESFKGKLDLAISNPPYIPKDTYEKLPKEVKNFEPRIALLGGEDGLQHIREIIQKAPIF
CHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHCCCCCEEEECCCHHHHHHHHHHHHCCEE
LKEKGWLILENHFDQGEKVKQLFIKNKFTSIEIVNDLSGIGRFTIGRYK
EECCCCEEEECCCCCHHHHHHHHHHCCCCEEEEEHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Protoporphyrinogen IX; O2

Specific reaction: protoporphyrinogen-IX + 1.5 O2 = protoporphyrin-IX + 3 H2O

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]