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The map label for this gene is dusB [H]

Identifier: 78484786

GI number: 78484786

Start: 493889

End: 494851

Strand: Reverse

Name: dusB [H]

Synonym: Tcr_0441

Alternate gene names: 78484786

Gene position: 494851-493889 (Counterclockwise)

Preceding gene: 78484787

Following gene: 78484785

Centisome position: 20.38

GC content: 46.83

Gene sequence:

>963_bases
ATGATGACGTTATTTGACTCTAGTACCCCCACGATTGCCCTGGCACCCATGGCAGGCATTACCGATCGGGTTTATCGTGA
CATTTGTCGAGAATCCGGTGCCAGTTATGCCGTGTCAGAAATGGTCGCTTCCAAAAAGCAGTTACGCGAATCGGCGAAAT
CGTCAACCCGCCATGCGGATAAAACGGAAACGTCTCCTCGCATTGTCCAGTTGCTTGGAACCCACCCAGAAGAATTGGTG
GATGCAGCGTTATGGCAACAATCCCAGGGGGCCGACATCATCGATCTCAACATGGGATGCCCAGCAAAAAAAGTGTGCGA
TGTGGCCGCAGGTTCTGCTTTAATGGGCCAACCTGAACGGGTTGCTGATATTTTTAAAAGTCTGACCGAGGCCATTGACC
TTCCGGTTACGGTTAAAATTCGAACTGGAACCGATGTGGATAACATCAATGCCGTCGAAATCGCCAAACTGGCGCAAGAA
TTGGGCCTGAAAGCCATCACTATTCATGGACGCACCCGCGCCGATAAATTTAATGGCCAGGCCGAATATGATACCATCAA
AGCCGTCAAACAGGCGGTTGACATCCCAGTTATTGCAAACGGTGATATTTGCAGCCCAAAAGAGGCTCAATTTGTATTAA
AATATACCGGTTCAGATGGCATTATGATTGGTCGAGCCGCCCAAGGATATCCTTGGATTTTCAGAGAGATAGCACACTTT
TTAAAGACTGGGGATTGTTTACCCTCCCCGAGTTTTGCTGAATTCCAGCAAGTGATGACCCGACACTTTTCAGAACTGGA
AATACTTTATGGCGAGCATTTAGGCCATAAAATCGCTCGAAAACATCTGGGCTGGTATAGCCAATACCTTCCGAACGGTA
CAGAGCTAAGAAAAAGATTTAATCGGCTTGAGAATACTCAGGCACAACTCAACTTAATCAACCATTACTTTGATACTTTA
TGA

Upstream 100 bases:

>100_bases
TTTTATCAGCAACAAGGGTTTGAGTTTTTAGCTCAGAATGACTTGGATGAATGGTCTCAGCTGAGCTTTACTAAACAAGT
GACCTCTTAAGTACTTCAAC

Downstream 100 bases:

>100_bases
CATTACTATCTGATAAAAATCCAGCCAGCCAAGCACTCAGCACGCATGTCACCATGACGCTGAAAAACTATTTTGAAACG
CTGGAAGGTGAAGTACCGAG

Product: dihydrouridine synthase TIM-barrel protein nifR3

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MMTLFDSSTPTIALAPMAGITDRVYRDICRESGASYAVSEMVASKKQLRESAKSSTRHADKTETSPRIVQLLGTHPEELV
DAALWQQSQGADIIDLNMGCPAKKVCDVAAGSALMGQPERVADIFKSLTEAIDLPVTVKIRTGTDVDNINAVEIAKLAQE
LGLKAITIHGRTRADKFNGQAEYDTIKAVKQAVDIPVIANGDICSPKEAQFVLKYTGSDGIMIGRAAQGYPWIFREIAHF
LKTGDCLPSPSFAEFQQVMTRHFSELEILYGEHLGHKIARKHLGWYSQYLPNGTELRKRFNRLENTQAQLNLINHYFDTL

Sequences:

>Translated_320_residues
MMTLFDSSTPTIALAPMAGITDRVYRDICRESGASYAVSEMVASKKQLRESAKSSTRHADKTETSPRIVQLLGTHPEELV
DAALWQQSQGADIIDLNMGCPAKKVCDVAAGSALMGQPERVADIFKSLTEAIDLPVTVKIRTGTDVDNINAVEIAKLAQE
LGLKAITIHGRTRADKFNGQAEYDTIKAVKQAVDIPVIANGDICSPKEAQFVLKYTGSDGIMIGRAAQGYPWIFREIAHF
LKTGDCLPSPSFAEFQQVMTRHFSELEILYGEHLGHKIARKHLGWYSQYLPNGTELRKRFNRLENTQAQLNLINHYFDTL
>Mature_320_residues
MMTLFDSSTPTIALAPMAGITDRVYRDICRESGASYAVSEMVASKKQLRESAKSSTRHADKTETSPRIVQLLGTHPEELV
DAALWQQSQGADIIDLNMGCPAKKVCDVAAGSALMGQPERVADIFKSLTEAIDLPVTVKIRTGTDVDNINAVEIAKLAQE
LGLKAITIHGRTRADKFNGQAEYDTIKAVKQAVDIPVIANGDICSPKEAQFVLKYTGSDGIMIGRAAQGYPWIFREIAHF
LKTGDCLPSPSFAEFQQVMTRHFSELEILYGEHLGHKIARKHLGWYSQYLPNGTELRKRFNRLENTQAQLNLINHYFDTL

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family. DusB subfamily [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=323, Percent_Identity=30.030959752322, Blast_Score=115, Evalue=6e-26,
Organism=Homo sapiens, GI31742496, Length=234, Percent_Identity=31.1965811965812, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI239788462, Length=335, Percent_Identity=28.955223880597, Blast_Score=112, Evalue=4e-25,
Organism=Homo sapiens, GI40807366, Length=225, Percent_Identity=31.5555555555556, Blast_Score=104, Evalue=1e-22,
Organism=Homo sapiens, GI8923374, Length=172, Percent_Identity=30.2325581395349, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789660, Length=306, Percent_Identity=51.6339869281046, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1788462, Length=260, Percent_Identity=31.1538461538462, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI145693211, Length=331, Percent_Identity=29.607250755287, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI25144369, Length=305, Percent_Identity=28.8524590163934, Blast_Score=106, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17510279, Length=304, Percent_Identity=30.2631578947368, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17543114, Length=233, Percent_Identity=30.0429184549356, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17507177, Length=228, Percent_Identity=28.0701754385965, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6323560, Length=241, Percent_Identity=29.4605809128631, Blast_Score=99, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323437, Length=234, Percent_Identity=27.7777777777778, Blast_Score=90, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323433, Length=307, Percent_Identity=24.1042345276873, Blast_Score=80, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24580595, Length=281, Percent_Identity=30.9608540925267, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI19920448, Length=281, Percent_Identity=30.9608540925267, Blast_Score=121, Evalue=6e-28,
Organism=Drosophila melanogaster, GI24585320, Length=235, Percent_Identity=34.0425531914894, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI45549423, Length=146, Percent_Identity=37.6712328767123, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI19921524, Length=224, Percent_Identity=26.7857142857143, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR004652
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 35426; Mature: 35426

Theoretical pI: Translated: 7.00; Mature: 7.00

Prosite motif: PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTLFDSSTPTIALAPMAGITDRVYRDICRESGASYAVSEMVASKKQLRESAKSSTRHAD
CCEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KTETSPRIVQLLGTHPEELVDAALWQQSQGADIIDLNMGCPAKKVCDVAAGSALMGQPER
CCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCHHHCCCCHH
VADIFKSLTEAIDLPVTVKIRTGTDVDNINAVEIAKLAQELGLKAITIHGRTRADKFNGQ
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHCCCC
AEYDTIKAVKQAVDIPVIANGDICSPKEAQFVLKYTGSDGIMIGRAAQGYPWIFREIAHF
CHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCHHHHHHHHH
LKTGDCLPSPSFAEFQQVMTRHFSELEILYGEHLGHKIARKHLGWYSQYLPNGTELRKRF
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
NRLENTQAQLNLINHYFDTL
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMTLFDSSTPTIALAPMAGITDRVYRDICRESGASYAVSEMVASKKQLRESAKSSTRHAD
CCEEECCCCCCEEECCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
KTETSPRIVQLLGTHPEELVDAALWQQSQGADIIDLNMGCPAKKVCDVAAGSALMGQPER
CCCCCHHHHHHHCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHCCHHHCCCCHH
VADIFKSLTEAIDLPVTVKIRTGTDVDNINAVEIAKLAQELGLKAITIHGRTRADKFNGQ
HHHHHHHHHHHHCCCEEEEEECCCCCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHCCCC
AEYDTIKAVKQAVDIPVIANGDICSPKEAQFVLKYTGSDGIMIGRAAQGYPWIFREIAHF
CHHHHHHHHHHHHCCCEEECCCCCCCCCCEEEEEEECCCCEEECCCCCCCCHHHHHHHHH
LKTGDCLPSPSFAEFQQVMTRHFSELEILYGEHLGHKIARKHLGWYSQYLPNGTELRKRF
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
NRLENTQAQLNLINHYFDTL
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]