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The map label for this gene is aroE

Identifier: 78484498

GI number: 78484498

Start: 185412

End: 186245

Strand: Reverse

Name: aroE

Synonym: Tcr_0153

Alternate gene names: 78484498

Gene position: 186245-185412 (Counterclockwise)

Preceding gene: 78484499

Following gene: 78484495

Centisome position: 7.67

GC content: 48.68

Gene sequence:

>834_bases
ATGACAGATTTATATGCCGTAGTGGGCAACCCGATTGCCCATTCAAAGTCGCCTCTGATCCATCGTCTATTTGCAGAACA
AACCGATCAGGATCTGGTTTATGAAGCGTTATTGATCGATACCGAAGACACTACGTTTCAGTTTGCCATCTCCGACCTGA
TCGCAAGAGGCTACCGAGGAATCAATATTACCGTACCGTTTAAACTTGACGCGTTTGAACTAGCGGATGAATTGAGCCCT
CGTGCTGAGGTAGCGCATGCCGTGAACACTTTCAGTTTTAAGGACGGTAAAATTTTTGGCGACAACACGGATGGCATTGG
CTTAGTGACTGACATCGAAGAAAATGCCAATCGTCCATTTAAAGACCAGAAAGTTTTAATACTAGGAGCAGGCGGCGCTG
TACAGGGTATTTTGCAGCCATTACTGGAAAAACAACCAGGCCTGGTTCACATTGCCAATCGCACCGCCAAACGAGCAGAA
GTGCTTGGAAAACGATTCGAAACCTTCTCACCCATCACCAGCAGTGACTGGGAAGGCATTCCCGACCAAGCTTATGACAT
TATCATCAACGGCACTTCGGCCAGCTTGGAAGGCAAACTGCCCCCGATTAATTCAAATGTAGTGGGACAGGACAGTCTAG
TCTATGACATGATGTACGGAGCCGAACCGACCATTTTCATGCAATGGGCACAGCAACACCAACCCTCTTGCCAAGTCAGA
GATGGATTGGGAATGCTGGTTGGACAAGCCGCCGAGGCTTTCTATATTTGGCGAGGCGTGCGCCCCCAAACGCAAAGCGT
GATCGATGAAGTCCGTCGCTTAATTCAGGCCTGA

Upstream 100 bases:

>100_bases
GTCTTGGAGACCTTATTAAGCTGTAAACGTGCAGGGGCAGACGGCATCCTGACCTATTACGCCAAGGTCGTCGCACAATG
GTTAGCGGAAAAATAATGCC

Downstream 100 bases:

>100_bases
TCTCGTTCAAGCTCGTCGTAGCACACCGTCCATCAGTGTGCTGCGCCTGTACAATTCATATAAGGGCAGAACTCCACTTC
CGACATGGGGCGAGCCAACA

Product: shikimate 5-dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 277; Mature: 276

Protein sequence:

>277_residues
MTDLYAVVGNPIAHSKSPLIHRLFAEQTDQDLVYEALLIDTEDTTFQFAISDLIARGYRGINITVPFKLDAFELADELSP
RAEVAHAVNTFSFKDGKIFGDNTDGIGLVTDIEENANRPFKDQKVLILGAGGAVQGILQPLLEKQPGLVHIANRTAKRAE
VLGKRFETFSPITSSDWEGIPDQAYDIIINGTSASLEGKLPPINSNVVGQDSLVYDMMYGAEPTIFMQWAQQHQPSCQVR
DGLGMLVGQAAEAFYIWRGVRPQTQSVIDEVRRLIQA

Sequences:

>Translated_277_residues
MTDLYAVVGNPIAHSKSPLIHRLFAEQTDQDLVYEALLIDTEDTTFQFAISDLIARGYRGINITVPFKLDAFELADELSP
RAEVAHAVNTFSFKDGKIFGDNTDGIGLVTDIEENANRPFKDQKVLILGAGGAVQGILQPLLEKQPGLVHIANRTAKRAE
VLGKRFETFSPITSSDWEGIPDQAYDIIINGTSASLEGKLPPINSNVVGQDSLVYDMMYGAEPTIFMQWAQQHQPSCQVR
DGLGMLVGQAAEAFYIWRGVRPQTQSVIDEVRRLIQA
>Mature_276_residues
TDLYAVVGNPIAHSKSPLIHRLFAEQTDQDLVYEALLIDTEDTTFQFAISDLIARGYRGINITVPFKLDAFELADELSPR
AEVAHAVNTFSFKDGKIFGDNTDGIGLVTDIEENANRPFKDQKVLILGAGGAVQGILQPLLEKQPGLVHIANRTAKRAEV
LGKRFETFSPITSSDWEGIPDQAYDIIINGTSASLEGKLPPINSNVVGQDSLVYDMMYGAEPTIFMQWAQQHQPSCQVRD
GLGMLVGQAAEAFYIWRGVRPQTQSVIDEVRRLIQA

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; fourth step. [C]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family

Homologues:

Organism=Escherichia coli, GI1789675, Length=277, Percent_Identity=42.5992779783394, Blast_Score=205, Evalue=3e-54,
Organism=Escherichia coli, GI1787983, Length=278, Percent_Identity=31.294964028777, Blast_Score=101, Evalue=4e-23,
Organism=Saccharomyces cerevisiae, GI6320332, Length=294, Percent_Identity=25.1700680272109, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROE_THICR (Q31JC4)

Other databases:

- EMBL:   CP000109
- RefSeq:   YP_390423.1
- HSSP:   Q9SQT8
- ProteinModelPortal:   Q31JC4
- SMR:   Q31JC4
- STRING:   Q31JC4
- GeneID:   3762445
- GenomeReviews:   CP000109_GR
- KEGG:   tcx:Tcr_0153
- NMPDR:   fig|317025.3.peg.105
- eggNOG:   COG0169
- HOGENOM:   HBG553408
- OMA:   DQYVVFG
- PhylomeDB:   Q31JC4
- BioCyc:   TCRU317025:TCR_0153-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00222
- InterPro:   IPR016040
- InterPro:   IPR011342
- InterPro:   IPR013708
- InterPro:   IPR022893
- InterPro:   IPR006151
- Gene3D:   G3DSA:3.40.50.720
- TIGRFAMs:   TIGR00507

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N

EC number: =1.1.1.25

Molecular weight: Translated: 30534; Mature: 30403

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: NA

Important sites: ACT_SITE 68-68

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDLYAVVGNPIAHSKSPLIHRLFAEQTDQDLVYEALLIDTEDTTFQFAISDLIARGYRG
CCCEEEECCCCCCCCCCHHHHHHHHHCCCHHHEEEEEEECCCCCCHHHHHHHHHHCCCCC
INITVPFKLDAFELADELSPRAEVAHAVNTFSFKDGKIFGDNTDGIGLVTDIEENANRPF
EEEEEEEECCHHHHHHHCCCHHHHHHHHHCEEECCCEEECCCCCCEEEEEECHHCCCCCC
KDQKVLILGAGGAVQGILQPLLEKQPGLVHIANRTAKRAEVLGKRFETFSPITSSDWEGI
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PDQAYDIIINGTSASLEGKLPPINSNVVGQDSLVYDMMYGAEPTIFMQWAQQHQPSCQVR
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEC
DGLGMLVGQAAEAFYIWRGVRPQTQSVIDEVRRLIQA
CCHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TDLYAVVGNPIAHSKSPLIHRLFAEQTDQDLVYEALLIDTEDTTFQFAISDLIARGYRG
CCEEEECCCCCCCCCCHHHHHHHHHCCCHHHEEEEEEECCCCCCHHHHHHHHHHCCCCC
INITVPFKLDAFELADELSPRAEVAHAVNTFSFKDGKIFGDNTDGIGLVTDIEENANRPF
EEEEEEEECCHHHHHHHCCCHHHHHHHHHCEEECCCEEECCCCCCEEEEEECHHCCCCCC
KDQKVLILGAGGAVQGILQPLLEKQPGLVHIANRTAKRAEVLGKRFETFSPITSSDWEGI
CCCEEEEEECCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCC
PDQAYDIIINGTSASLEGKLPPINSNVVGQDSLVYDMMYGAEPTIFMQWAQQHQPSCQVR
CCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCEEC
DGLGMLVGQAAEAFYIWRGVRPQTQSVIDEVRRLIQA
CCHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA