Click here to switch to the map view.

The map label for this gene is ycgF [H]

Identifier: 78484374

GI number: 78484374

Start: 34387

End: 35154

Strand: Reverse

Name: ycgF [H]

Synonym: Tcr_0028

Alternate gene names: 78484374

Gene position: 35154-34387 (Counterclockwise)

Preceding gene: 78484376

Following gene: 78484373

Centisome position: 1.45

GC content: 38.15

Gene sequence:

>768_bases
ATGACGACCCATTTCCCAATAGAAACGATCAAATCCGGCTGTTCTCAGTGTCATACTCCGCTAGGATTTGAGTTTACCTT
TGCGTTTCAACCGATTATTCATGTTTTTAACAAGGAAATATGGGGATATGAAGCCTTGGTTCGCGGCCTGGAAGAAAAAA
CCGCTTGGAGTGTTTTAAAGCGGGTAAACGATGATAACCGTTATGCTTTTGATCAAGCTTGCCGAACCAAGGCCATTGCA
CTCGCCAGTCAATTAAAGTTAGATAAAATGTTGAGTATCAATTTTTTACCGAATGCGGTTTATGAACCAAAACATTGTAT
TCAAAGTACGTTAAAGGCGGCTAAAGAACATCAATTTCCATTAGAAAAAATCATGTTTGAAATCACCGAGTCAGAGCAGG
TGGTGGATCGAGCTCATCTGACGAATATTTTTGAATATTATCAATCTCAAGGCTTTACCACTGCGTTGGATGATTTTGGA
GAAGGCCATGCGGGCTTGAATATGTTGGCCAGTTTTGTACCCAATATTTTAAAAATTGATATGGAACTGGTAAAGAACGT
TGATCAAAGTCCGGTTAAGCAAGCGATTACAAAAAATTTAATTCAAATGTGCAATGATCTTAATGTCACGGTATTAGCCG
AAGGAATAGAACGGATTGAAGAAGTGAACTATTTTAAAGATCTTGGCGTGCCATTAATGCAAGGTTATTTCTTCGCTAAG
CCAGGTTTTGAGAATTTACCAACAGTGAATTTTTCATCGTTAGATTAA

Upstream 100 bases:

>100_bases
TCTTAGTTTGAACGCTAAATAACGCATGAATGACCTTCTGTGCATTTGTTTTTATCTTTCTCTATAATGATTTTAATTAT
CAACAGAGTTTACTTTTATT

Downstream 100 bases:

>100_bases
TGGTTTTTAAAAATCTTTAAAAACCAATTGCTTATAAAATAAGTAAACCTTCTTATTTCTACCTATCACTATCCTTAGTA
TTTACACATATATTTTTTGT

Product: diguanylate phosphodiesterase

Products: NA

Alternate protein names: Blrp [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MTTHFPIETIKSGCSQCHTPLGFEFTFAFQPIIHVFNKEIWGYEALVRGLEEKTAWSVLKRVNDDNRYAFDQACRTKAIA
LASQLKLDKMLSINFLPNAVYEPKHCIQSTLKAAKEHQFPLEKIMFEITESEQVVDRAHLTNIFEYYQSQGFTTALDDFG
EGHAGLNMLASFVPNILKIDMELVKNVDQSPVKQAITKNLIQMCNDLNVTVLAEGIERIEEVNYFKDLGVPLMQGYFFAK
PGFENLPTVNFSSLD

Sequences:

>Translated_255_residues
MTTHFPIETIKSGCSQCHTPLGFEFTFAFQPIIHVFNKEIWGYEALVRGLEEKTAWSVLKRVNDDNRYAFDQACRTKAIA
LASQLKLDKMLSINFLPNAVYEPKHCIQSTLKAAKEHQFPLEKIMFEITESEQVVDRAHLTNIFEYYQSQGFTTALDDFG
EGHAGLNMLASFVPNILKIDMELVKNVDQSPVKQAITKNLIQMCNDLNVTVLAEGIERIEEVNYFKDLGVPLMQGYFFAK
PGFENLPTVNFSSLD
>Mature_254_residues
TTHFPIETIKSGCSQCHTPLGFEFTFAFQPIIHVFNKEIWGYEALVRGLEEKTAWSVLKRVNDDNRYAFDQACRTKAIAL
ASQLKLDKMLSINFLPNAVYEPKHCIQSTLKAAKEHQFPLEKIMFEITESEQVVDRAHLTNIFEYYQSQGFTTALDDFGE
GHAGLNMLASFVPNILKIDMELVKNVDQSPVKQAITKNLIQMCNDLNVTVLAEGIERIEEVNYFKDLGVPLMQGYFFAKP
GFENLPTVNFSSLD

Specific function: Binds to and releases the YcgE repressor from its bound DNA target in a blue-light-dependent (470 nm) fashion. A shift to low temperature also triggers a YcgF-medisted relief of repression by YcgE, suggesting YcgF may serve as a thermometer. Blue light ma

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 EAL domain [H]

Homologues:

Organism=Escherichia coli, GI1787410, Length=226, Percent_Identity=31.4159292035398, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1790496, Length=227, Percent_Identity=29.0748898678414, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI87081980, Length=233, Percent_Identity=25.3218884120172, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1788502, Length=231, Percent_Identity=26.4069264069264, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787541, Length=137, Percent_Identity=30.6569343065693, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI226510982, Length=231, Percent_Identity=24.2424242424242, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI87081845, Length=162, Percent_Identity=28.3950617283951, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87081921, Length=240, Percent_Identity=27.0833333333333, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788849, Length=150, Percent_Identity=27.3333333333333, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786507, Length=119, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI87082096, Length=152, Percent_Identity=28.2894736842105, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1787055, Length=231, Percent_Identity=26.8398268398268, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001792
- InterPro:   IPR007024
- InterPro:   IPR001633 [H]

Pfam domain/function: PF04940 BLUF; PF00563 EAL [H]

EC number: NA

Molecular weight: Translated: 29000; Mature: 28868

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS50883 EAL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCCCCCCCC
>Mature Secondary Structure 
TTHFPIETIKSGCSQCHTPLGFEFTFAFQPIIHVFNKEIWGYEALVRGLEEKTAWSVLK
CCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RVNDDNRYAFDQACRTKAIALASQLKLDKMLSINFLPNAVYEPKHCIQSTLKAAKEHQFP
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCC
LEKIMFEITESEQVVDRAHLTNIFEYYQSQGFTTALDDFGEGHAGLNMLASFVPNILKID
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHH
MELVKNVDQSPVKQAITKNLIQMCNDLNVTVLAEGIERIEEVNYFKDLGVPLMQGYFFAK
HHHHHCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCC
PGFENLPTVNFSSLD
CCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]