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The map label for this gene is potD [H]

Identifier: 78358712

GI number: 78358712

Start: 3632965

End: 3634011

Strand: Direct

Name: potD [H]

Synonym: Dde_3673

Alternate gene names: 78358712

Gene position: 3632965-3634011 (Clockwise)

Preceding gene: 78358711

Following gene: 78358722

Centisome position: 97.39

GC content: 53.96

Gene sequence:

>1047_bases
ATGAAAAAGCTCTTCGCCGTCATCCTGACGGTCATGCTGTGTCCGTTTTTTGCCCAGAACGCTCTGGCGCAGGATGAAAA
AATCCTCAATGTGTACAACTGGTCGGAATACATTCCGCAGGATGTGCTGACGCAGTTCACGGAAGAAACCGGCATCGAAA
TCAACTACACCACGTTTGAATCCAACGAAGCCATGTACGCCAAGCTCAAACTGCTGGACGGCAAAGGCTACGATGTGGTG
GTGCCTTCTACCTATTTTGTCACGCTGATGCGCGAACAGGGCCTGCTGGCCAGAATAGACACCGGCAAGCTGGATAACTT
CAAGCATATTGACCCGTTTGTCCTGCACCAGCCCTTTGACCCGAAAAACGAATATTCCGTGCCTTACATGTGGGGTTCCA
CCAGCCTGCTGGTGAATACCGATCATGTCGATCCCGCATCCGTAACCAGCTGGCACGACCTGCTGCGTCCGGAATTCAAG
GGCAAGATCATTCTGTCGGACGATCTGCGCGACACCATGGGCATGGCTCTGCTGGCCACCGGCCATTCGGTGAACAGCCG
CAGCGATGCAGAGCTCAAAGAAGCGTACGAGTTTCTGAAACAGCTCTACCCTTCCGTACGCGTGTTTGATGTGACTGCCA
CCAAGCAGGCGTTCGCCAGCGAGGAAGTCATCATCGGCATGTCATGGAACGGCGATGCCACCGTGCTGACGCAGGAAATG
CCCAATCTGCAGTACATCTATCCCCGAGAAGGCGCCCCCCTGTGGCTGGACAGCCTGTGCATTCCCAAAAATGCCGAGCA
CAAAGACAACGCCCATACGTTCATCAACTTTCTGCTGCGGCCCGACATTGCCAAGCGCATAGTGGAAGAATATCTGTATT
CCACCCCCAACAAAGCCGCGTGGGAACTGCTGCCCGAAGACCTGAAAAACAGCCGGGCGGTTTCCCCCACAGCGCAGGAT
CTCGAAAAAAGCGAATTCTGCGACTCAGTGGGCCCCGCACTGCAGATATATGAAAAATACTGGCAGATGCTGAAAACCAG
AAGTTAA

Upstream 100 bases:

>100_bases
GCGTAAAGCCCGACATCAACGCGCTGAGCGCGGTTCTGTTCTGCCTGACAATCGTACTGGTACTGCTGGCCCACACCCTT
AGCAAGGCAAGGAGACGCCC

Downstream 100 bases:

>100_bases
CGCATTGCGCCACCCGCACATAAAGCCCGCAGGCATAAGCCTGCGGGCTTTTTTTCATACACGCATTGACAAAAAAAACT
GCGGAGGCTCTCCGCAGGAC

Product: polyamine ABC transporter periplasmic polyamine-binding protein

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: SPBP [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKKLFAVILTVMLCPFFAQNALAQDEKILNVYNWSEYIPQDVLTQFTEETGIEINYTTFESNEAMYAKLKLLDGKGYDVV
VPSTYFVTLMREQGLLARIDTGKLDNFKHIDPFVLHQPFDPKNEYSVPYMWGSTSLLVNTDHVDPASVTSWHDLLRPEFK
GKIILSDDLRDTMGMALLATGHSVNSRSDAELKEAYEFLKQLYPSVRVFDVTATKQAFASEEVIIGMSWNGDATVLTQEM
PNLQYIYPREGAPLWLDSLCIPKNAEHKDNAHTFINFLLRPDIAKRIVEEYLYSTPNKAAWELLPEDLKNSRAVSPTAQD
LEKSEFCDSVGPALQIYEKYWQMLKTRS

Sequences:

>Translated_348_residues
MKKLFAVILTVMLCPFFAQNALAQDEKILNVYNWSEYIPQDVLTQFTEETGIEINYTTFESNEAMYAKLKLLDGKGYDVV
VPSTYFVTLMREQGLLARIDTGKLDNFKHIDPFVLHQPFDPKNEYSVPYMWGSTSLLVNTDHVDPASVTSWHDLLRPEFK
GKIILSDDLRDTMGMALLATGHSVNSRSDAELKEAYEFLKQLYPSVRVFDVTATKQAFASEEVIIGMSWNGDATVLTQEM
PNLQYIYPREGAPLWLDSLCIPKNAEHKDNAHTFINFLLRPDIAKRIVEEYLYSTPNKAAWELLPEDLKNSRAVSPTAQD
LEKSEFCDSVGPALQIYEKYWQMLKTRS
>Mature_348_residues
MKKLFAVILTVMLCPFFAQNALAQDEKILNVYNWSEYIPQDVLTQFTEETGIEINYTTFESNEAMYAKLKLLDGKGYDVV
VPSTYFVTLMREQGLLARIDTGKLDNFKHIDPFVLHQPFDPKNEYSVPYMWGSTSLLVNTDHVDPASVTSWHDLLRPEFK
GKIILSDDLRDTMGMALLATGHSVNSRSDAELKEAYEFLKQLYPSVRVFDVTATKQAFASEEVIIGMSWNGDATVLTQEM
PNLQYIYPREGAPLWLDSLCIPKNAEHKDNAHTFINFLLRPDIAKRIVEEYLYSTPNKAAWELLPEDLKNSRAVSPTAQD
LEKSEFCDSVGPALQIYEKYWQMLKTRS

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787367, Length=325, Percent_Identity=47.6923076923077, Blast_Score=325, Evalue=2e-90,
Organism=Escherichia coli, GI1787078, Length=316, Percent_Identity=32.2784810126582, Blast_Score=170, Evalue=1e-43,
Organism=Escherichia coli, GI1787711, Length=319, Percent_Identity=24.1379310344828, Blast_Score=73, Evalue=3e-14,

Paralogues:

None

Copy number: 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 460 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 39795; Mature: 39795

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLFAVILTVMLCPFFAQNALAQDEKILNVYNWSEYIPQDVLTQFTEETGIEINYTTFE
CHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHHCCHHHHHHHHHHCCCEEEEEEEC
SNEAMYAKLKLLDGKGYDVVVPSTYFVTLMREQGLLARIDTGKLDNFKHIDPFVLHQPFD
CCCEEEEEEEEECCCCCEEEECCCEEEEEHHCCCCEEEECCCCCCCCCCCCCEEEECCCC
PKNEYSVPYMWGSTSLLVNTDHVDPASVTSWHDLLRPEFKGKIILSDDLRDTMGMALLAT
CCCCCCCCEEECCCEEEEECCCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHCEEEEEE
GHSVNSRSDAELKEAYEFLKQLYPSVRVFDVTATKQAFASEEVIIGMSWNGDATVLTQEM
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCCEEEEEECCCCEEEEEECC
PNLQYIYPREGAPLWLDSLCIPKNAEHKDNAHTFINFLLRPDIAKRIVEEYLYSTPNKAA
CCCEEEECCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
WELLPEDLKNSRAVSPTAQDLEKSEFCDSVGPALQIYEKYWQMLKTRS
HHHCHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKLFAVILTVMLCPFFAQNALAQDEKILNVYNWSEYIPQDVLTQFTEETGIEINYTTFE
CHHHHHHHHHHHHHHHHHHHHHHCCHHHEEEHHHHHHCCHHHHHHHHHHCCCEEEEEEEC
SNEAMYAKLKLLDGKGYDVVVPSTYFVTLMREQGLLARIDTGKLDNFKHIDPFVLHQPFD
CCCEEEEEEEEECCCCCEEEECCCEEEEEHHCCCCEEEECCCCCCCCCCCCCEEEECCCC
PKNEYSVPYMWGSTSLLVNTDHVDPASVTSWHDLLRPEFKGKIILSDDLRDTMGMALLAT
CCCCCCCCEEECCCEEEEECCCCCCCCHHHHHHHHCCCCCCEEEECCCHHHHHCEEEEEE
GHSVNSRSDAELKEAYEFLKQLYPSVRVFDVTATKQAFASEEVIIGMSWNGDATVLTQEM
CCCCCCCCCHHHHHHHHHHHHHCCCEEEEEEEHHHHHHCCCCEEEEEECCCCEEEEEECC
PNLQYIYPREGAPLWLDSLCIPKNAEHKDNAHTFINFLLRPDIAKRIVEEYLYSTPNKAA
CCCEEEECCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHCCCCHHH
WELLPEDLKNSRAVSPTAQDLEKSEFCDSVGPALQIYEKYWQMLKTRS
HHHCHHHHCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1939142; 8905232; 9278503; 9298646; 8194593; 8621624; 8897598 [H]