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The map label for this gene is purD [H]

Identifier: 78358497

GI number: 78358497

Start: 3419328

End: 3420599

Strand: Direct

Name: purD [H]

Synonym: Dde_3458

Alternate gene names: 78358497

Gene position: 3419328-3420599 (Clockwise)

Preceding gene: 78358494

Following gene: 78358498

Centisome position: 91.67

GC content: 62.03

Gene sequence:

>1272_bases
GTGAAGATCCTGATTGTCGGCTCCGGCGGACGCGAACATGCCCTCGCATGGAAGCTGCGCCAAAGTCCGCTCGTCGACGA
GCTGTTCATCGCCCCAGGTAACGGCGGTACCGCCCTTGAAGGCACAAACGTGCCCATCGCCGAAGACGATCTGCCTGCCA
TCGTGGCCTTTGCCCGCGAACACAGCATAGACCTTGTGGTACCCGGGCCCGAACTCCCCCTTGTTCTCGGCATCAAGGAT
TCCCTTGCCGCCGCAGGCATCGCCTGCTTCGGCCCCAACGCTTTTTCCGCCCAGCTCGAAGGCAGCAAGGCCTTTGCCAA
GCACATCATGCGCGAAGCAGGCGTACCCACTGCCGACTTCGGCGTGTTTGACGATTTTTACGAAGCCCGTGACTATGTGC
TGCGTCATGGCGCGCCCATCGTGGTAAAAGCCGACGGGCTGGCCGCGGGCAAAGGTGTTGTGGTGGCCCGCACCACGGAA
GAAGCCATCGAAGCCCTTGATGACATCATGGTCAAAAAAATCTACGGCAGCGCCGGAGCCCATGTGGTGGTGGAAGCCGC
ACTGGCCGGAGAGGAAGCATCGTTTCTCGCGCTGGTGGACGGCGAAACCATAGTGCCCCTGCCTTCGGCGCAGGACCACA
AGGCCGTGGGCGAGGGCGACACCGGTCCCAACACCGGCGGCATGGGCGCATACAGCCCCGCACCGGTCCTGCCCGCAGAC
CGCTGGGACGAGATGACCGAACTGGTCATACGCCCCATCGTACGCACGCTGGCCGCCAAAGATACACCTTTCTGCGGCGT
GCTGTACGCCGGACTCATGATGACCGCGGACGGACCTTATGTGCTGGAGTATAATGTCCGCTTCGGCGATCCGGAATGCC
AGCCGCTGCTCATGAGGCTGGACGGCGATCTTGCAGCCATCATGATGGCTGCCGCCACAGGCAGACTGGACAGCGTGCCC
GTGACATACAATCCCCGGACAGCGCTTGGAGTGGTCATCGCGGCACAGGGGTATCCGGGCAGCTATGATTCGGGCATGGA
AATCAGCGGCATAGAAGAAGCAGAAGCAGACGGTGCCAAAGTGTTTCAGGCGGGCACCAGCCTGAAAGACGGCAAAACGG
TCTCCCGCGGGGGACGTGTGCTGTGCGTCACCGCGCTGGGCGACACGCTGGCTCAGGCGCAGCAAAAAGCCTATGCCGCC
GCAGACCGCGTACGCATGGAAAAAAGCTACTACCGTCGTGATATAGGCAACAAAGGCCTCTCACGGGCGTAA

Upstream 100 bases:

>100_bases
CGAAAAGCGCCCCTACGGGGCCTGACCGTGCTAACACGAAACACGGCGGGGGAAAACCCCGGCCACACCGCCCCTGCCAC
AGACATCCGGAGGCCCACCC

Downstream 100 bases:

>100_bases
TACATCTTCAGCGGGGCACAGCTCCGCAACCGGGACTGCGGGCCGCCTGTGCGGCCCGCTCTGATATCCACCATCAACCT
GTATGGAGTAGACAATGCCT

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 423; Mature: 423

Protein sequence:

>423_residues
MKILIVGSGGREHALAWKLRQSPLVDELFIAPGNGGTALEGTNVPIAEDDLPAIVAFAREHSIDLVVPGPELPLVLGIKD
SLAAAGIACFGPNAFSAQLEGSKAFAKHIMREAGVPTADFGVFDDFYEARDYVLRHGAPIVVKADGLAAGKGVVVARTTE
EAIEALDDIMVKKIYGSAGAHVVVEAALAGEEASFLALVDGETIVPLPSAQDHKAVGEGDTGPNTGGMGAYSPAPVLPAD
RWDEMTELVIRPIVRTLAAKDTPFCGVLYAGLMMTADGPYVLEYNVRFGDPECQPLLMRLDGDLAAIMMAAATGRLDSVP
VTYNPRTALGVVIAAQGYPGSYDSGMEISGIEEAEADGAKVFQAGTSLKDGKTVSRGGRVLCVTALGDTLAQAQQKAYAA
ADRVRMEKSYYRRDIGNKGLSRA

Sequences:

>Translated_423_residues
MKILIVGSGGREHALAWKLRQSPLVDELFIAPGNGGTALEGTNVPIAEDDLPAIVAFAREHSIDLVVPGPELPLVLGIKD
SLAAAGIACFGPNAFSAQLEGSKAFAKHIMREAGVPTADFGVFDDFYEARDYVLRHGAPIVVKADGLAAGKGVVVARTTE
EAIEALDDIMVKKIYGSAGAHVVVEAALAGEEASFLALVDGETIVPLPSAQDHKAVGEGDTGPNTGGMGAYSPAPVLPAD
RWDEMTELVIRPIVRTLAAKDTPFCGVLYAGLMMTADGPYVLEYNVRFGDPECQPLLMRLDGDLAAIMMAAATGRLDSVP
VTYNPRTALGVVIAAQGYPGSYDSGMEISGIEEAEADGAKVFQAGTSLKDGKTVSRGGRVLCVTALGDTLAQAQQKAYAA
ADRVRMEKSYYRRDIGNKGLSRA
>Mature_423_residues
MKILIVGSGGREHALAWKLRQSPLVDELFIAPGNGGTALEGTNVPIAEDDLPAIVAFAREHSIDLVVPGPELPLVLGIKD
SLAAAGIACFGPNAFSAQLEGSKAFAKHIMREAGVPTADFGVFDDFYEARDYVLRHGAPIVVKADGLAAGKGVVVARTTE
EAIEALDDIMVKKIYGSAGAHVVVEAALAGEEASFLALVDGETIVPLPSAQDHKAVGEGDTGPNTGGMGAYSPAPVLPAD
RWDEMTELVIRPIVRTLAAKDTPFCGVLYAGLMMTADGPYVLEYNVRFGDPECQPLLMRLDGDLAAIMMAAATGRLDSVP
VTYNPRTALGVVIAAQGYPGSYDSGMEISGIEEAEADGAKVFQAGTSLKDGKTVSRGGRVLCVTALGDTLAQAQQKAYAA
ADRVRMEKSYYRRDIGNKGLSRA

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=424, Percent_Identity=48.3490566037736, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI209869995, Length=424, Percent_Identity=48.3490566037736, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI209869993, Length=424, Percent_Identity=48.3490566037736, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI28416899, Length=424, Percent_Identity=48.3490566037736, Blast_Score=398, Evalue=1e-111,
Organism=Escherichia coli, GI1790438, Length=426, Percent_Identity=51.1737089201878, Blast_Score=428, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17567511, Length=421, Percent_Identity=42.0427553444181, Blast_Score=315, Evalue=3e-86,
Organism=Saccharomyces cerevisiae, GI6321203, Length=442, Percent_Identity=43.6651583710407, Blast_Score=340, Evalue=3e-94,
Organism=Drosophila melanogaster, GI62471619, Length=429, Percent_Identity=44.0559440559441, Blast_Score=316, Evalue=2e-86,
Organism=Drosophila melanogaster, GI24582400, Length=432, Percent_Identity=43.9814814814815, Blast_Score=315, Evalue=4e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44332; Mature: 44332

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIVGSGGREHALAWKLRQSPLVDELFIAPGNGGTALEGTNVPIAEDDLPAIVAFARE
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHH
HSIDLVVPGPELPLVLGIKDSLAAAGIACFGPNAFSAQLEGSKAFAKHIMREAGVPTADF
CCEEEEECCCCCCEEEECCHHHHHCCEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCCC
GVFDDFYEARDYVLRHGAPIVVKADGLAAGKGVVVARTTEEAIEALDDIMVKKIYGSAGA
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCC
HVVVEAALAGEEASFLALVDGETIVPLPSAQDHKAVGEGDTGPNTGGMGAYSPAPVLPAD
EEEEEEECCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RWDEMTELVIRPIVRTLAAKDTPFCGVLYAGLMMTADGPYVLEYNVRFGDPECQPLLMRL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCEEEECCCCEEEEEEEEECCCCCCEEEEEE
DGDLAAIMMAAATGRLDSVPVTYNPRTALGVVIAAQGYPGSYDSGMEISGIEEAEADGAK
CCCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCC
VFQAGTSLKDGKTVSRGGRVLCVTALGDTLAQAQQKAYAAADRVRMEKSYYRRDIGNKGL
EEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SRA
CCC
>Mature Secondary Structure
MKILIVGSGGREHALAWKLRQSPLVDELFIAPGNGGTALEGTNVPIAEDDLPAIVAFARE
CEEEEEECCCCCCEEEEEECCCCCCCEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHH
HSIDLVVPGPELPLVLGIKDSLAAAGIACFGPNAFSAQLEGSKAFAKHIMREAGVPTADF
CCEEEEECCCCCCEEEECCHHHHHCCEEEECCCCCEEEECCHHHHHHHHHHHCCCCCCCC
GVFDDFYEARDYVLRHGAPIVVKADGLAAGKGVVVARTTEEAIEALDDIMVKKIYGSAGA
CHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCEEEEECHHHHHHHHHHHHHHHHHCCCCC
HVVVEAALAGEEASFLALVDGETIVPLPSAQDHKAVGEGDTGPNTGGMGAYSPAPVLPAD
EEEEEEECCCCCCCEEEEECCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RWDEMTELVIRPIVRTLAAKDTPFCGVLYAGLMMTADGPYVLEYNVRFGDPECQPLLMRL
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCEEEECCCCEEEEEEEEECCCCCCEEEEEE
DGDLAAIMMAAATGRLDSVPVTYNPRTALGVVIAAQGYPGSYDSGMEISGIEEAEADGAK
CCCHHHHHHHHHCCCCCCCCEEECCCCEEEEEEEECCCCCCCCCCCEECCCCCCCCCCCC
VFQAGTSLKDGKTVSRGGRVLCVTALGDTLAQAQQKAYAAADRVRMEKSYYRRDIGNKGL
EEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
SRA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]