Click here to switch to the map view.

The map label for this gene is phnE [H]

Identifier: 78358366

GI number: 78358366

Start: 3311634

End: 3312731

Strand: Direct

Name: phnE [H]

Synonym: Dde_3326

Alternate gene names: 78358366

Gene position: 3311634-3312731 (Clockwise)

Preceding gene: 78358365

Following gene: 78358368

Centisome position: 88.78

GC content: 57.83

Gene sequence:

>1098_bases
ATGTCAGAACTGACCCTCGAACAGCTCACCCCGAGAAAGACCCTCACCCAGAGGGTCCTTCTCGGACTCATGAGCCTCAC
TGTAGCAGCAGTCCTTGCCGTAACGTACGTGTGGTGCGGCATCGACCCCGTCAAACTGTACGAAAAACGCAACAACGCCG
TGGAATACCTGTTCGGGCACAGCATTTCAGATGCCGACAGAACGGCTGCCATGGAACAGGCACGCAGGCTGCCCGCCATC
ATCATCCATCAGGAAGCCATGGACGAAGTGCGCAAAGAAATGGCTGCCAAAGGCTCCGCGGACTTTACGGAAATGACCGG
ACGCGGCAACGAACTGGCCGAAAAAAGGCTGGCCGCGATGGACCCTGCGGCATTCGACGCCATAGTGCAGGAAGAATACG
AACGGGTGCTGGATGAAAAGCGCGGCGGATTCTTTCCTCCGGAAATGCGTACCGGCCCCCTGATGCAGTACAGCCGCGCC
CTGATTGAAACCATTGCCATAGCCATATGGGGCACGCTGCTGGCTTTTGTGGCAGCCATTCCCGCATCCATGCTGGCGGC
GCGCAACACGCTGTGTCTTATTCTGCCGGGCAGTTCGCCCGCCCGCAAAAAGCTGCGCCGCAGCATCCAGTTTGCCATGC
GGCGGCTGCTCGACTTCTGCCGCGGATTCAATGAATTCGTCATGGCCCTCATTTTCGTGGCAGTCATCGGTCTGGGGCCT
TATGCCGGGGTGCTGGCCCTTGCCATCCACTCGTTCGGCATTCTCAGCAAGGTGTTCAGCGAAGGTATCGAAGCCATCGA
ACCCGGTCAGGTGGAAGCCGTGGAAGCCAGCGGCGCCAGCTCGGTACAGACCATTGTCTTTTCCGTGCTGCCGCAGATGA
TGCCGCTCATTGTCAGCTACAGCCTGCTGCGCTTTGAAACCAACGTGCGCAGCGCCACCATTCTGGGATTTGTGGGTGCC
GGCGGCATAGGCTTTCTGATTTTCGACAAGCTGAACGGCTACCTGTACCGCGAAGTAAGCACCATGATGATTCTGGTCAT
CCTCATGGTCACCGTCATCGATTACATGTGCGGCATCATCCGCAGCAGGTTTGTATAA

Upstream 100 bases:

>100_bases
AGTACACCGACGACAGCACCTACGACATCATCCGCTACCTGAAGCGCCTGAAAAACGAACTGGCAGCCAAATAGCACCAG
CCCATCCACCGGAAAAGAGC

Downstream 100 bases:

>100_bases
CCATGCGCAGTTCAGCCGCCTGTACCGCCGCAGACGTTTTACGCGGCATCAATGACAAACTGGATGCGGTCGGCCGCCCA
GACGCACACCCCGTATTCCA

Product: phosphonate ABC transporter inner membrane protein

Products: 3-phospho-serine [Cytoplasm]; phosphite [Cytoplasm]; ADP; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 364

Protein sequence:

>365_residues
MSELTLEQLTPRKTLTQRVLLGLMSLTVAAVLAVTYVWCGIDPVKLYEKRNNAVEYLFGHSISDADRTAAMEQARRLPAI
IIHQEAMDEVRKEMAAKGSADFTEMTGRGNELAEKRLAAMDPAAFDAIVQEEYERVLDEKRGGFFPPEMRTGPLMQYSRA
LIETIAIAIWGTLLAFVAAIPASMLAARNTLCLILPGSSPARKKLRRSIQFAMRRLLDFCRGFNEFVMALIFVAVIGLGP
YAGVLALAIHSFGILSKVFSEGIEAIEPGQVEAVEASGASSVQTIVFSVLPQMMPLIVSYSLLRFETNVRSATILGFVGA
GGIGFLIFDKLNGYLYREVSTMMILVILMVTVIDYMCGIIRSRFV

Sequences:

>Translated_365_residues
MSELTLEQLTPRKTLTQRVLLGLMSLTVAAVLAVTYVWCGIDPVKLYEKRNNAVEYLFGHSISDADRTAAMEQARRLPAI
IIHQEAMDEVRKEMAAKGSADFTEMTGRGNELAEKRLAAMDPAAFDAIVQEEYERVLDEKRGGFFPPEMRTGPLMQYSRA
LIETIAIAIWGTLLAFVAAIPASMLAARNTLCLILPGSSPARKKLRRSIQFAMRRLLDFCRGFNEFVMALIFVAVIGLGP
YAGVLALAIHSFGILSKVFSEGIEAIEPGQVEAVEASGASSVQTIVFSVLPQMMPLIVSYSLLRFETNVRSATILGFVGA
GGIGFLIFDKLNGYLYREVSTMMILVILMVTVIDYMCGIIRSRFV
>Mature_364_residues
SELTLEQLTPRKTLTQRVLLGLMSLTVAAVLAVTYVWCGIDPVKLYEKRNNAVEYLFGHSISDADRTAAMEQARRLPAII
IHQEAMDEVRKEMAAKGSADFTEMTGRGNELAEKRLAAMDPAAFDAIVQEEYERVLDEKRGGFFPPEMRTGPLMQYSRAL
IETIAIAIWGTLLAFVAAIPASMLAARNTLCLILPGSSPARKKLRRSIQFAMRRLLDFCRGFNEFVMALIFVAVIGLGPY
AGVLALAIHSFGILSKVFSEGIEAIEPGQVEAVEASGASSVQTIVFSVLPQMMPLIVSYSLLRFETNVRSATILGFVGAG
GIGFLIFDKLNGYLYREVSTMMILVILMVTVIDYMCGIIRSRFV

Specific function: Part of the binding-protein-dependent transport system for phosphonates; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG3639

COG function: function code P; ABC-type phosphate/phosphonate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR005769 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 40182; Mature: 40051

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.9 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELTLEQLTPRKTLTQRVLLGLMSLTVAAVLAVTYVWCGIDPVKLYEKRNNAVEYLFGH
CCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCC
SISDADRTAAMEQARRLPAIIIHQEAMDEVRKEMAAKGSADFTEMTGRGNELAEKRLAAM
CCCHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHC
DPAAFDAIVQEEYERVLDEKRGGFFPPEMRTGPLMQYSRALIETIAIAIWGTLLAFVAAI
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PASMLAARNTLCLILPGSSPARKKLRRSIQFAMRRLLDFCRGFNEFVMALIFVAVIGLGP
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCH
YAGVLALAIHSFGILSKVFSEGIEAIEPGQVEAVEASGASSVQTIVFSVLPQMMPLIVSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SLLRFETNVRSATILGFVGAGGIGFLIFDKLNGYLYREVSTMMILVILMVTVIDYMCGII
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRFV
HHHCC
>Mature Secondary Structure 
SELTLEQLTPRKTLTQRVLLGLMSLTVAAVLAVTYVWCGIDPVKLYEKRNNAVEYLFGH
CCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHHHCC
SISDADRTAAMEQARRLPAIIIHQEAMDEVRKEMAAKGSADFTEMTGRGNELAEKRLAAM
CCCHHHHHHHHHHHHCCCHHEEEHHHHHHHHHHHHHCCCCCHHHHCCCCHHHHHHHHHHC
DPAAFDAIVQEEYERVLDEKRGGFFPPEMRTGPLMQYSRALIETIAIAIWGTLLAFVAAI
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PASMLAARNTLCLILPGSSPARKKLRRSIQFAMRRLLDFCRGFNEFVMALIFVAVIGLGP
HHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHCCCH
YAGVLALAIHSFGILSKVFSEGIEAIEPGQVEAVEASGASSVQTIVFSVLPQMMPLIVSY
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHHHHHHHHHHHHHHHH
SLLRFETNVRSATILGFVGAGGIGFLIFDKLNGYLYREVSTMMILVILMVTVIDYMCGII
HHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSRFV
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]