Click here to switch to the map view.

The map label for this gene is pyrE [H]

Identifier: 78358052

GI number: 78358052

Start: 2997521

End: 2998081

Strand: Direct

Name: pyrE [H]

Synonym: Dde_3012

Alternate gene names: 78358052

Gene position: 2997521-2998081 (Clockwise)

Preceding gene: 78358051

Following gene: 78358053

Centisome position: 80.36

GC content: 55.08

Gene sequence:

>561_bases
ATGAACGATCTCAAACGCCGTCTTGCAAAAATACTTGCAGAAAAATCATATAAAGAAGGCGACTTTACACTCGCCTCCGG
CAAGAAAAGCGATTATTATTTCGACTGCCGTCAGACCGCCCTGCATGCGGAAGGTTCGTGGCTTATCGGCAACCTGTTCT
TTGAAATGATACAGAACATGGACGGTGCCGGTGTGGGCGGCATGACGCTGGGCGCAGACCCGCTGGTCACTGCCGTCACG
GTGATATCGCACGAAAAAGGCCGCCCCCTGCCGGGCCTTATTGTACGTAAACAAGCCAAAGGGCACGGCACAGGACAGTA
CGTGGAAGGACTGGCCAACTTCAGTCAGGGCGACACCGTCATCATGCTTGAAGACGTGGTCACCACCGGCGGTTCGGTGC
TTACAGCCATCGAACGCATTGAAGCCGCAGGGCTGAAAGTAGCCGCAGTGTGCTGCGTGCTGGACAGAGAAGAAGGCGGC
CGCCAGGCACTGGAAGAAAAGGGCTATGCCCTGCATTCCATTTTTACCCGCAAGCAGCTTGTGGAAGCTGCACGCTCATA
A

Upstream 100 bases:

>100_bases
GAGGTGCTTGATGCGCTGTTCGACGCAGGGTACTACCTGCGTCACGAAGACATGATCTTTGACCGCGTTTTTGCGGGCTA
ACCGCTCAGGAGAGCAATAC

Downstream 100 bases:

>100_bases
CGTTGCAACCGTGCGGAGAGCCCTTTGCGGCTCTCCGCGTACCATTCAGCCACACATACTTATATGACCGGAACACGCAG
ACCCCTTGCAAGCCTGCTTA

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 186; Mature: 186

Protein sequence:

>186_residues
MNDLKRRLAKILAEKSYKEGDFTLASGKKSDYYFDCRQTALHAEGSWLIGNLFFEMIQNMDGAGVGGMTLGADPLVTAVT
VISHEKGRPLPGLIVRKQAKGHGTGQYVEGLANFSQGDTVIMLEDVVTTGGSVLTAIERIEAAGLKVAAVCCVLDREEGG
RQALEEKGYALHSIFTRKQLVEAARS

Sequences:

>Translated_186_residues
MNDLKRRLAKILAEKSYKEGDFTLASGKKSDYYFDCRQTALHAEGSWLIGNLFFEMIQNMDGAGVGGMTLGADPLVTAVT
VISHEKGRPLPGLIVRKQAKGHGTGQYVEGLANFSQGDTVIMLEDVVTTGGSVLTAIERIEAAGLKVAAVCCVLDREEGG
RQALEEKGYALHSIFTRKQLVEAARS
>Mature_186_residues
MNDLKRRLAKILAEKSYKEGDFTLASGKKSDYYFDCRQTALHAEGSWLIGNLFFEMIQNMDGAGVGGMTLGADPLVTAVT
VISHEKGRPLPGLIVRKQAKGHGTGQYVEGLANFSQGDTVIMLEDVVTTGGSVLTAIERIEAAGLKVAAVCCVLDREEGG
RQALEEKGYALHSIFTRKQLVEAARS

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=170, Percent_Identity=35.8823529411765, Blast_Score=101, Evalue=4e-22,
Organism=Escherichia coli, GI1790073, Length=198, Percent_Identity=31.3131313131313, Blast_Score=64, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17554964, Length=175, Percent_Identity=37.7142857142857, Blast_Score=105, Evalue=2e-23,
Organism=Caenorhabditis elegans, GI17508631, Length=168, Percent_Identity=31.547619047619, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI17933654, Length=178, Percent_Identity=35.3932584269663, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 20063; Mature: 20063

Theoretical pI: Translated: 6.93; Mature: 6.93

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDLKRRLAKILAEKSYKEGDFTLASGKKSDYYFDCRQTALHAEGSWLIGNLFFEMIQNM
CCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHCC
DGAGVGGMTLGADPLVTAVTVISHEKGRPLPGLIVRKQAKGHGTGQYVEGLANFSQGDTV
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEE
IMLEDVVTTGGSVLTAIERIEAAGLKVAAVCCVLDREEGGRQALEEKGYALHSIFTRKQL
EEEEHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHH
VEAARS
HHHHCC
>Mature Secondary Structure
MNDLKRRLAKILAEKSYKEGDFTLASGKKSDYYFDCRQTALHAEGSWLIGNLFFEMIQNM
CCHHHHHHHHHHHHCCCCCCCEEEECCCCCCEEEHHHHHHHCCCCCCHHHHHHHHHHHCC
DGAGVGGMTLGADPLVTAVTVISHEKGRPLPGLIVRKQAKGHGTGQYVEGLANFSQGDTV
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCCEE
IMLEDVVTTGGSVLTAIERIEAAGLKVAAVCCVLDREEGGRQALEEKGYALHSIFTRKQL
EEEEHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHHHHH
VEAARS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11759840 [H]