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The map label for this gene is purB [H]

Identifier: 78358051

GI number: 78358051

Start: 2996206

End: 2997501

Strand: Direct

Name: purB [H]

Synonym: Dde_3011

Alternate gene names: 78358051

Gene position: 2996206-2997501 (Clockwise)

Preceding gene: 78358050

Following gene: 78358052

Centisome position: 80.32

GC content: 57.48

Gene sequence:

>1296_bases
ATGATCGAACGGTACACCCGTCCCGAAATGGGGCAGCTCTGGACGCTGGAAAACAGATTCCGCGTCTGGCTTGAAGTGGA
AATCGCCGTCTGCGAAGCCTGGAACCGCCTGGGCGTGATAGACGACGAAACAATGACCACCATACGGGAAAAAGCAGACT
TCGATGTGGAGCGCATCCTTGAAATCGAAGAACAGACCCGCCATGACGTCATCGCCTTTCTGACCGCCGTGGAAGAAAAG
GTAGGCCCTGCCGCCCGGTTCATCCATCTGGGCTGCACTTCATCTGACATCGTGGATACCGCCAACGGCGTGATGCTGAG
CCGTGCGGGCGACATGGTGGTGCAGGGTATAGAGCGTCTGCTGGTCACGCTGGAAAAACTGGCCCGTGCCAACAAGGGAC
GCCTGTGCATGGGCAGAACCCACGGAATTCACGCCGAACCCACCAGCTTCGGACTGAAAATGGCAGGCTACTACGCCGAA
TTCCGCCGGCATCTGGAACGGGTGAAGGCCGGCGTGGAAAGCGTACGCGTGGGCAAGATTTCCGGTGCCGTGGGCACCTA
CGCCATGCTCAACCCCGAAGTGGAGCGCATCACCTGCCAGATTCTGGGACTGCACGCAGACCCCATCTCCACCCAGATCA
TCCAGCGTGACAGACACGCGCATTTCTTTACCGCTCTGGCGGTCTGTGCCGGCGGCATAGAACGCCTGTGCGTTGAACTG
CGCCACCTGCAGCGCACTGAAGTGCTGGAAGCCGAAGAAGGTTTCCGCAAGGGGCAGAAAGGTTCTTCGGCCATGCCGCA
CAAAAAGAACCCCATCTCGGCGGAAAACATGACCGGTCTTGCACGGCTTATCCGCACCAACTCCGTTGCCGCCATGGAAA
ATCAGGCACTGTGGCACGAACGCGATATATCCCACTCTTCCGTGGAACGCGTCATCATGCCCGATTCGACCATACTTATG
GATTATGTGCTGCACCGCCTGTGCAATCTGCTCGAAAACCTGCGTGTTATTCCCGAAAACATGGACCGCAACCTTATGAG
CTCATACGGTCTGTTCTTTTCACAGCGGGTGCTCACCGCTCTGGTGGAGTCGGGCATGGCACGGCAGGAAGCCTATGTCA
TGGTGCAGAAAGCAGCCATGAAAAGCTGGGATACCGGCACCAGTTTTCCCGAAAATGTCCGGGCCGATGCAGACATAACC
GCACATCTGCAGCCTGAGGTGCTTGATGCGCTGTTCGACGCAGGGTACTACCTGCGTCACGAAGACATGATCTTTGACCG
CGTTTTTGCGGGCTAA

Upstream 100 bases:

>100_bases
CAGGCCAGGCATCCTGATTTTTTGGCAGCCTCCGGCTGCCTTTTTCATACCCACAGGGCGCTTAGCCCACAACCGCGTAG
CGACTATAGAGACCAGACCG

Downstream 100 bases:

>100_bases
CCGCTCAGGAGAGCAATACATGAACGATCTCAAACGCCGTCTTGCAAAAATACTTGCAGAAAAATCATATAAAGAAGGCG
ACTTTACACTCGCCTCCGGC

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MIERYTRPEMGQLWTLENRFRVWLEVEIAVCEAWNRLGVIDDETMTTIREKADFDVERILEIEEQTRHDVIAFLTAVEEK
VGPAARFIHLGCTSSDIVDTANGVMLSRAGDMVVQGIERLLVTLEKLARANKGRLCMGRTHGIHAEPTSFGLKMAGYYAE
FRRHLERVKAGVESVRVGKISGAVGTYAMLNPEVERITCQILGLHADPISTQIIQRDRHAHFFTALAVCAGGIERLCVEL
RHLQRTEVLEAEEGFRKGQKGSSAMPHKKNPISAENMTGLARLIRTNSVAAMENQALWHERDISHSSVERVIMPDSTILM
DYVLHRLCNLLENLRVIPENMDRNLMSSYGLFFSQRVLTALVESGMARQEAYVMVQKAAMKSWDTGTSFPENVRADADIT
AHLQPEVLDALFDAGYYLRHEDMIFDRVFAG

Sequences:

>Translated_431_residues
MIERYTRPEMGQLWTLENRFRVWLEVEIAVCEAWNRLGVIDDETMTTIREKADFDVERILEIEEQTRHDVIAFLTAVEEK
VGPAARFIHLGCTSSDIVDTANGVMLSRAGDMVVQGIERLLVTLEKLARANKGRLCMGRTHGIHAEPTSFGLKMAGYYAE
FRRHLERVKAGVESVRVGKISGAVGTYAMLNPEVERITCQILGLHADPISTQIIQRDRHAHFFTALAVCAGGIERLCVEL
RHLQRTEVLEAEEGFRKGQKGSSAMPHKKNPISAENMTGLARLIRTNSVAAMENQALWHERDISHSSVERVIMPDSTILM
DYVLHRLCNLLENLRVIPENMDRNLMSSYGLFFSQRVLTALVESGMARQEAYVMVQKAAMKSWDTGTSFPENVRADADIT
AHLQPEVLDALFDAGYYLRHEDMIFDRVFAG
>Mature_431_residues
MIERYTRPEMGQLWTLENRFRVWLEVEIAVCEAWNRLGVIDDETMTTIREKADFDVERILEIEEQTRHDVIAFLTAVEEK
VGPAARFIHLGCTSSDIVDTANGVMLSRAGDMVVQGIERLLVTLEKLARANKGRLCMGRTHGIHAEPTSFGLKMAGYYAE
FRRHLERVKAGVESVRVGKISGAVGTYAMLNPEVERITCQILGLHADPISTQIIQRDRHAHFFTALAVCAGGIERLCVEL
RHLQRTEVLEAEEGFRKGQKGSSAMPHKKNPISAENMTGLARLIRTNSVAAMENQALWHERDISHSSVERVIMPDSTILM
DYVLHRLCNLLENLRVIPENMDRNLMSSYGLFFSQRVLTALVESGMARQEAYVMVQKAAMKSWDTGTSFPENVRADADIT
AHLQPEVLDALFDAGYYLRHEDMIFDRVFAG

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=438, Percent_Identity=26.027397260274, Blast_Score=146, Evalue=4e-35,
Organism=Homo sapiens, GI183227688, Length=383, Percent_Identity=26.1096605744125, Blast_Score=141, Evalue=1e-33,
Organism=Escherichia coli, GI1787376, Length=288, Percent_Identity=31.9444444444444, Blast_Score=96, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17508577, Length=384, Percent_Identity=26.0416666666667, Blast_Score=116, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI32564234, Length=328, Percent_Identity=26.219512195122, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=387, Percent_Identity=27.1317829457364, Blast_Score=140, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24647570, Length=434, Percent_Identity=26.7281105990783, Blast_Score=132, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48662; Mature: 48662

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
6.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIERYTRPEMGQLWTLENRFRVWLEVEIAVCEAWNRLGVIDDETMTTIREKADFDVERIL
CCCCCCCCCCCCEEEECCCEEEEEEEEEHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
EIEEQTRHDVIAFLTAVEEKVGPAARFIHLGCTSSDIVDTANGVMLSRAGDMVVQGIERL
HHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHCCCCEEEECCHHHHHHHHHHH
LVTLEKLARANKGRLCMGRTHGIHAEPTSFGLKMAGYYAEFRRHLERVKAGVESVRVGKI
HHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
SGAVGTYAMLNPEVERITCQILGLHADPISTQIIQRDRHAHFFTALAVCAGGIERLCVEL
CCCCCCHHHCCCHHHHEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
RHLQRTEVLEAEEGFRKGQKGSSAMPHKKNPISAENMTGLARLIRTNSVAAMENQALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
RDISHSSVERVIMPDSTILMDYVLHRLCNLLENLRVIPENMDRNLMSSYGLFFSQRVLTA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
LVESGMARQEAYVMVQKAAMKSWDTGTSFPENVRADADITAHLQPEVLDALFDAGYYLRH
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCEEEH
EDMIFDRVFAG
HHHHHHHHHCC
>Mature Secondary Structure
MIERYTRPEMGQLWTLENRFRVWLEVEIAVCEAWNRLGVIDDETMTTIREKADFDVERIL
CCCCCCCCCCCCEEEECCCEEEEEEEEEHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHH
EIEEQTRHDVIAFLTAVEEKVGPAARFIHLGCTSSDIVDTANGVMLSRAGDMVVQGIERL
HHHHHHHHHHHHHHHHHHHHCCCHHHEEEECCCCCHHHHCCCCEEEECCHHHHHHHHHHH
LVTLEKLARANKGRLCMGRTHGIHAEPTSFGLKMAGYYAEFRRHLERVKAGVESVRVGKI
HHHHHHHHCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEE
SGAVGTYAMLNPEVERITCQILGLHADPISTQIIQRDRHAHFFTALAVCAGGIERLCVEL
CCCCCCHHHCCCHHHHEEEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
RHLQRTEVLEAEEGFRKGQKGSSAMPHKKNPISAENMTGLARLIRTNSVAAMENQALWHE
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
RDISHSSVERVIMPDSTILMDYVLHRLCNLLENLRVIPENMDRNLMSSYGLFFSQRVLTA
CCCCHHHHCEEECCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHHHHHHH
LVESGMARQEAYVMVQKAAMKSWDTGTSFPENVRADADITAHLQPEVLDALFDAGYYLRH
HHHHCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCEEEH
EDMIFDRVFAG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]