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The map label for this gene is purT

Identifier: 78357809

GI number: 78357809

Start: 2767044

End: 2768225

Strand: Reverse

Name: purT

Synonym: Dde_2767

Alternate gene names: 78357809

Gene position: 2768225-2767044 (Counterclockwise)

Preceding gene: 78357812

Following gene: 78357808

Centisome position: 74.21

GC content: 61.68

Gene sequence:

>1182_bases
ATGACAGTCATCGGTACCCCTTTAAGTCCTGTTAGAACCAGAATAATGCTGCTCGGCTCCGGCGAGCTGGGCAAAGAGGT
CGCCATTGAGGCAATGCGGCTGGGAGCGGAAGTTGTGGCGGTGGACCGCTATGCCGGTGCTCCCGCCATGCAGGTGGCCC
ACCGCTCGCACGTTATTTCCATGCTGGACGCGGATGCGTTGCGGCGGCTTATTGCCGAAGAACGCCCTGACTTTGTGGTG
CCGGAGATAGAGGCCATCGCCACGGACGTGCTGGAAGAACTGGAAAGTGATTCTCTTACCGTGGTGCCGACGGCCCGCGC
CGCCCGCCTGACCATGAACAGAGAAGGCATACGCCGTCTGGCTGCGGAAGAGCTGGGGCTCAGAACTTCTCCGTACCGCT
TTGCGCAGACAGAGGATGAATACCGCCGCGCAGTGGAGGAGCTGGGCATGCCCTGTGTGGTCAAGCCGGTCATGAGCTCT
TCGGGCAAAGGCCAGAGCGTGGTGCGCAGTGCGGATGACATCGCAGATGCGTGGACGTATGCCCAGTCCGGCGGCCGGGC
CGGCGGCGGACGGGTCATCGTCGAATCATTCGTCGATTTTGATTACGAGATCACACTGCTTACCGTGCGGCATTGCAACG
GTACTTCTTTCTGCGAGCCCATCGGCCACAGGCAGGAAGACGGAGACTACCGTCAGTCGTGGCAGCCGCAACCCATGAGC
GCGGCCGCGCTGGAGCAGGCCCGGCATATGGCGCAGGCCGTGACACAGGCGCTGGGCGGTTACGGATTGTTCGGGGTGGA
GCTGTTCATCAAAGGTGATACCGTCTGGTTCAGCGAGGTGTCCCCGCGGCCTCATGATACAGGGCTTGTTACCATAGCTT
CTCAGAATCTTTCGGAATTTGCGCTGCATGTGCGTGCCGTTCTGGGGCTGCCGGTGCCGGTCATCCGCCAGTACGGGCCC
GCTGCATCCAGCGTTATTCTGGCAGAAGGCAGTTCTGCCCGGACCCGCTTCGGTAATCTGCAGGAAGCGCTCGGCGAGCC
GGATACTTCTCTGCTGCTGTTCGGCAAGCCGGAAGTGCAGGGCCGCAGACGCATGGGGGTGGCTCTGGCTCTGGGCGAAA
CAGTGGAGCAGGCTGTGGAAAAGGCGTGCCGCGTTTCAGACGCCGTCAAGGTCATGCTGTAA

Upstream 100 bases:

>100_bases
CCTTGACTGGTGGAGTATGCCAAATGAGCGCAGCAGCTTTGCGCCACAATGTTTTTTATCAATATTCCGCAGCATACACC
CGCAACTCAGGAGCTTTGCC

Downstream 100 bases:

>100_bases
TCCGCATTGTTCCGGAACGCCCGCGCAGCCGCGCGGGCGTTTTGCGTTATGCCGTGCTTTGTGCGGCACAAATGTTACTG
ACGCGGCAGGAGCCGTGCGG

Product: phosphoribosylglycinamide formyltransferase 2

Products: 5'-Phosphoribosyl-N-Formylglycinamide; Pyrophosphate; acetylphosphate; ADP [C]

Alternate protein names: GART 2; 5'-phosphoribosylglycinamide transformylase 2; Formate-dependent GAR transformylase; GAR transformylase 2

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MTVIGTPLSPVRTRIMLLGSGELGKEVAIEAMRLGAEVVAVDRYAGAPAMQVAHRSHVISMLDADALRRLIAEERPDFVV
PEIEAIATDVLEELESDSLTVVPTARAARLTMNREGIRRLAAEELGLRTSPYRFAQTEDEYRRAVEELGMPCVVKPVMSS
SGKGQSVVRSADDIADAWTYAQSGGRAGGGRVIVESFVDFDYEITLLTVRHCNGTSFCEPIGHRQEDGDYRQSWQPQPMS
AAALEQARHMAQAVTQALGGYGLFGVELFIKGDTVWFSEVSPRPHDTGLVTIASQNLSEFALHVRAVLGLPVPVIRQYGP
AASSVILAEGSSARTRFGNLQEALGEPDTSLLLFGKPEVQGRRRMGVALALGETVEQAVEKACRVSDAVKVML

Sequences:

>Translated_393_residues
MTVIGTPLSPVRTRIMLLGSGELGKEVAIEAMRLGAEVVAVDRYAGAPAMQVAHRSHVISMLDADALRRLIAEERPDFVV
PEIEAIATDVLEELESDSLTVVPTARAARLTMNREGIRRLAAEELGLRTSPYRFAQTEDEYRRAVEELGMPCVVKPVMSS
SGKGQSVVRSADDIADAWTYAQSGGRAGGGRVIVESFVDFDYEITLLTVRHCNGTSFCEPIGHRQEDGDYRQSWQPQPMS
AAALEQARHMAQAVTQALGGYGLFGVELFIKGDTVWFSEVSPRPHDTGLVTIASQNLSEFALHVRAVLGLPVPVIRQYGP
AASSVILAEGSSARTRFGNLQEALGEPDTSLLLFGKPEVQGRRRMGVALALGETVEQAVEKACRVSDAVKVML
>Mature_392_residues
TVIGTPLSPVRTRIMLLGSGELGKEVAIEAMRLGAEVVAVDRYAGAPAMQVAHRSHVISMLDADALRRLIAEERPDFVVP
EIEAIATDVLEELESDSLTVVPTARAARLTMNREGIRRLAAEELGLRTSPYRFAQTEDEYRRAVEELGMPCVVKPVMSSS
GKGQSVVRSADDIADAWTYAQSGGRAGGGRVIVESFVDFDYEITLLTVRHCNGTSFCEPIGHRQEDGDYRQSWQPQPMSA
AALEQARHMAQAVTQALGGYGLFGVELFIKGDTVWFSEVSPRPHDTGLVTIASQNLSEFALHVRAVLGLPVPVIRQYGPA
ASSVILAEGSSARTRFGNLQEALGEPDTSLLLFGKPEVQGRRRMGVALALGETVEQAVEKACRVSDAVKVML

Specific function: Catalyzes two reactions:the first one is the production of beta-formyl glycinamide ribonucleotide (GAR) from formate, ATP and beta GAR; the second, a side reaction, is the production of acetyl phosphate and ADP from acetate and ATP

COG id: COG0027

COG function: function code F; Formate-dependent phosphoribosylglycinamide formyltransferase (GAR transformylase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Escherichia coli, GI1788155, Length=391, Percent_Identity=67.2634271099744, Blast_Score=532, Evalue=1e-152,
Organism=Escherichia coli, GI1786733, Length=305, Percent_Identity=26.2295081967213, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324702, Length=361, Percent_Identity=24.3767313019391, Blast_Score=103, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURT_DESDG (Q30XN3)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389258.1
- ProteinModelPortal:   Q30XN3
- SMR:   Q30XN3
- STRING:   Q30XN3
- GeneID:   3757790
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2767
- NMPDR:   fig|207559.3.peg.3283
- eggNOG:   COG0027
- HOGENOM:   HBG516369
- OMA:   PDYIVPE
- ProtClustDB:   PRK09288
- BioCyc:   DDES207559:DDE_2767-MONOMER
- HAMAP:   MF_01643
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR005862
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20
- PANTHER:   PTHR23047:SF2
- TIGRFAMs:   TIGR01142

Pfam domain/function: PF02222 ATP-grasp; SSF52440 PreATP-grasp-like

EC number: 2.1.2.- [C]

Molecular weight: Translated: 42545; Mature: 42414

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50975 ATP_GRASP; PS00430 TONB_DEPENDENT_REC_1

Important sites: BINDING 82-82 BINDING 114-114 BINDING 155-155 BINDING 203-203 BINDING 267-267 BINDING 279-279 BINDING 286-286 BINDING 356-356

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVIGTPLSPVRTRIMLLGSGELGKEVAIEAMRLGAEVVAVDRYAGAPAMQVAHRSHVIS
CCEECCCCHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
MLDADALRRLIAEERPDFVVPEIEAIATDVLEELESDSLTVVPTARAARLTMNREGIRRL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHEECCHHHHHHH
AAEELGLRTSPYRFAQTEDEYRRAVEELGMPCVVKPVMSSSGKGQSVVRSADDIADAWTY
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQSGGRAGGGRVIVESFVDFDYEITLLTVRHCNGTSFCEPIGHRQEDGDYRQSWQPQPMS
HHCCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCHHHHCCCCCCCCCHHHCCCCCCCH
AAALEQARHMAQAVTQALGGYGLFGVELFIKGDTVWFSEVSPRPHDTGLVTIASQNLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHH
ALHVRAVLGLPVPVIRQYGPAASSVILAEGSSARTRFGNLQEALGEPDTSLLLFGKPEVQ
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCCEEEEECCCCCC
GRRRMGVALALGETVEQAVEKACRVSDAVKVML
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TVIGTPLSPVRTRIMLLGSGELGKEVAIEAMRLGAEVVAVDRYAGAPAMQVAHRSHVIS
CEECCCCHHHHEEEEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHH
MLDADALRRLIAEERPDFVVPEIEAIATDVLEELESDSLTVVPTARAARLTMNREGIRRL
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCEEEEECCHHHHEECCHHHHHHH
AAEELGLRTSPYRFAQTEDEYRRAVEELGMPCVVKPVMSSSGKGQSVVRSADDIADAWTY
HHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHHCCCCCCHHHHHHHHHHHHHHHH
AQSGGRAGGGRVIVESFVDFDYEITLLTVRHCNGTSFCEPIGHRQEDGDYRQSWQPQPMS
HHCCCCCCCCHHHHHHHHCCCEEEEEEEEEECCCCCHHHHCCCCCCCCCHHHCCCCCCCH
AAALEQARHMAQAVTQALGGYGLFGVELFIKGDTVWFSEVSPRPHDTGLVTIASQNLSEF
HHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCEEEEECCCCCCCCCCEEEEECCCHHHH
ALHVRAVLGLPVPVIRQYGPAASSVILAEGSSARTRFGNLQEALGEPDTSLLLFGKPEVQ
HHHHHHHHCCCHHHHHHCCCCCCEEEEECCCCCHHHHCCHHHHHCCCCCEEEEECCCCCC
GRRRMGVALALGETVEQAVEKACRVSDAVKVML
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Formate; 5'-Phospho-Ribosylglycinamide; acetate; ATP [C]

Specific reaction: Formate + ATP + 5'-Phospho-Ribosylglycinamide = 5'-Phosphoribosyl-N-Formylglycinamide + ADP + Pyrophosphate. acetate + ATP = acetylphosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA