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The map label for this gene is fliM [H]

Identifier: 78357750

GI number: 78357750

Start: 2709747

End: 2710727

Strand: Reverse

Name: fliM [H]

Synonym: Dde_2708

Alternate gene names: 78357750

Gene position: 2710727-2709747 (Counterclockwise)

Preceding gene: 78357751

Following gene: 78357734

Centisome position: 72.67

GC content: 53.52

Gene sequence:

>981_bases
ATGAACAAAATTCTGGCACAGGATGAAGTGGATGCCCTGTTACGGGGGCTTTCCGGCGGCGAGATAGAAACAGAATCCGA
TATACCGGAGGATGATTCCGGCATTGTGTCTTTTGACCTTGCCAATCAGGACAGAATCATCCGCGGGCGTATGCCGGTTC
TGGAAATCGTCAACGACCGCTTTGCCCGTCTGTGCACCAACGCGCTGAGCAACGCCGTGCGCAAGCGTGTGGAACTGAAC
CCCATATCCATAGATATGACCAAGTTCGGTGACTTTATGCGCTCGCTGCCCGTGCCTACCAGCATCAATATTTTCAAGAT
GGAACCGCTGCGCGGCAATGCCATCATCGTGGTCGATTCACGCCTTGTTTTCGCTCTGGTGGAAAACTTTTTCGGCGGGG
CAGGCAGTCAGCCCAAAATTGAAGGCCGCGAGTTCACGCGCATAGAACAGGCCATTGTGGACAGGGTTATCAAAATTGCG
CTTGATAACATGGAAGAATCGTGGCGGCCGGTGCATGAGGTGAACCTTGAGCTGGTCCGTTCTGAAATCAACCCGCAGTT
TGCTGCCATTGTGCCGCCCAGTGACGTTGTTGTGGTCATCACCTTTGAGGTGGAACTGGAATCGGCCATCGGTTCGCTGG
TTATGTGTCTGCCGTATGCCACCATCGAACCTATCCGTTCGAAGCTGCATGCCAGCTTTCAGACCGAGCGGCTGGAAGTG
GACCATGCATGGGTTGCCCGTCTGAAGGAGCGTCTGCTGGAAACTCCGGTGGAAATGAAAATACGCTTCGGGGAGACGAA
GATAACCGGCAACCAGCTGTTGCGGCTGAAAGTGGGCGACGTGCTGCTGCTTGATACCGATACTGATGATCTGCTGGAAG
GCACCGTGGAAGGGGTGCGCAAATTCTGGGGAATAAGCGGTATTGTCAAGGCTAATAAAGCATTTCAGGTCATCCGTGAG
GAAGAACCCAGATATACCTGA

Upstream 100 bases:

>100_bases
GAGCAAACGGCGGTATTTGCCGGTTGGTTCTGCCGGTTCTTTTTTATGATGTCCGTCCGTTCCGCGGGCGTGGTAACCAG
CACGGAGCCTGACTCTCTGT

Downstream 100 bases:

>100_bases
TATGCGCTGCCGGTTACGGATATTTCCGGAAAGAATGCAAGCGGCTTCGGCCGCTTTTTTTTGTCAGCTTTCGGGGGGGC
AGCATTCGTCCGGGTAGAAA

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNKILAQDEVDALLRGLSGGEIETESDIPEDDSGIVSFDLANQDRIIRGRMPVLEIVNDRFARLCTNALSNAVRKRVELN
PISIDMTKFGDFMRSLPVPTSINIFKMEPLRGNAIIVVDSRLVFALVENFFGGAGSQPKIEGREFTRIEQAIVDRVIKIA
LDNMEESWRPVHEVNLELVRSEINPQFAAIVPPSDVVVVITFEVELESAIGSLVMCLPYATIEPIRSKLHASFQTERLEV
DHAWVARLKERLLETPVEMKIRFGETKITGNQLLRLKVGDVLLLDTDTDDLLEGTVEGVRKFWGISGIVKANKAFQVIRE
EEPRYT

Sequences:

>Translated_326_residues
MNKILAQDEVDALLRGLSGGEIETESDIPEDDSGIVSFDLANQDRIIRGRMPVLEIVNDRFARLCTNALSNAVRKRVELN
PISIDMTKFGDFMRSLPVPTSINIFKMEPLRGNAIIVVDSRLVFALVENFFGGAGSQPKIEGREFTRIEQAIVDRVIKIA
LDNMEESWRPVHEVNLELVRSEINPQFAAIVPPSDVVVVITFEVELESAIGSLVMCLPYATIEPIRSKLHASFQTERLEV
DHAWVARLKERLLETPVEMKIRFGETKITGNQLLRLKVGDVLLLDTDTDDLLEGTVEGVRKFWGISGIVKANKAFQVIRE
EEPRYT
>Mature_326_residues
MNKILAQDEVDALLRGLSGGEIETESDIPEDDSGIVSFDLANQDRIIRGRMPVLEIVNDRFARLCTNALSNAVRKRVELN
PISIDMTKFGDFMRSLPVPTSINIFKMEPLRGNAIIVVDSRLVFALVENFFGGAGSQPKIEGREFTRIEQAIVDRVIKIA
LDNMEESWRPVHEVNLELVRSEINPQFAAIVPPSDVVVVITFEVELESAIGSLVMCLPYATIEPIRSKLHASFQTERLEV
DHAWVARLKERLLETPVEMKIRFGETKITGNQLLRLKVGDVLLLDTDTDDLLEGTVEGVRKFWGISGIVKANKAFQVIRE
EEPRYT

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=336, Percent_Identity=33.6309523809524, Blast_Score=190, Evalue=1e-49,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 36655; Mature: 36655

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKILAQDEVDALLRGLSGGEIETESDIPEDDSGIVSFDLANQDRIIRGRMPVLEIVNDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHH
FARLCTNALSNAVRKRVELNPISIDMTKFGDFMRSLPVPTSINIFKMEPLRGNAIIVVDS
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECC
RLVFALVENFFGGAGSQPKIEGREFTRIEQAIVDRVIKIALDNMEESWRPVHEVNLELVR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SEINPQFAAIVPPSDVVVVITFEVELESAIGSLVMCLPYATIEPIRSKLHASFQTERLEV
HHCCCCEEEEECCCCEEEEEEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHEEH
DHAWVARLKERLLETPVEMKIRFGETKITGNQLLRLKVGDVLLLDTDTDDLLEGTVEGVR
HHHHHHHHHHHHHCCCCEEEEEECCEEECCCEEEEEEECCEEEEECCCHHHHHHHHHHHH
KFWGISGIVKANKAFQVIREEEPRYT
HHHCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNKILAQDEVDALLRGLSGGEIETESDIPEDDSGIVSFDLANQDRIIRGRMPVLEIVNDR
CCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEECCCCCEEECCCHHHHHHHHH
FARLCTNALSNAVRKRVELNPISIDMTKFGDFMRSLPVPTSINIFKMEPLRGNAIIVVDS
HHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHCCCCCCEEEEEECCCCCCEEEEECC
RLVFALVENFFGGAGSQPKIEGREFTRIEQAIVDRVIKIALDNMEESWRPVHEVNLELVR
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SEINPQFAAIVPPSDVVVVITFEVELESAIGSLVMCLPYATIEPIRSKLHASFQTERLEV
HHCCCCEEEEECCCCEEEEEEEEEEEHHHHHHHHHHHCHHHHHHHHHHHHCCHHHHHEEH
DHAWVARLKERLLETPVEMKIRFGETKITGNQLLRLKVGDVLLLDTDTDDLLEGTVEGVR
HHHHHHHHHHHHHCCCCEEEEEECCEEECCCEEEEEEECCEEEEECCCHHHHHHHHHHHH
KFWGISGIVKANKAFQVIREEEPRYT
HHHCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10984043; 7622217 [H]