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The map label for this gene is gatB

Identifier: 78357657

GI number: 78357657

Start: 2630044

End: 2631474

Strand: Reverse

Name: gatB

Synonym: Dde_2615

Alternate gene names: 78357657

Gene position: 2631474-2630044 (Counterclockwise)

Preceding gene: 78357658

Following gene: 78357656

Centisome position: 70.54

GC content: 55.97

Gene sequence:

>1431_bases
ATGGCTGAATACGAAGTCGTCATAGGGCTGGAGGTGCACGCCCAGCTGCGCACCAGCTCGAAGCTGTTCTGCGCCTGTTC
CACGGAATTCGGAAGCGAACCCAATACCAACGTGTGTGAAATCTGTTCCGGCATGCCCGGCGTGCTGCCGGTGGTTAACA
GAAAAGCCGTGGAATACGCCGTAATGATGGGCATGGCTGTGGACTGCACCATCAATAACGATTCGGTGTTTGCACGTAAA
AACTATTTTTATCCCGATCTGCCCTACGGTTATCAGATTTCGCAGTTCGAGCGGCCGCTGTGCGAACACGGGCATCTTGA
TATTCCGGTGAACGGCGGCACAAAGCGCATCGGTATAACGCGTATACACATGGAAAATGATGCCGGTAAAAACATTCATT
CGGCTGCGGACAATGCCAGCTATGTGGATTTGAACCGTGCCGGAACGCCGCTGATTGAAATCGTCAGCGAACCGGATATG
CGCAGCGCGGAAGAAGCCGTGGCCTATCTGAAGGAACTGCGCTCCATACTGCTGTATCTCGGTATCTGTGACGGTAATAT
GGAAGAAGGCAGCTTTCGCTGCGACGCCAACGTTTCTTTGCGCCCTGCGGGACAGAAGGAATTCGGCACCCGCACGGAAC
TGAAAAACGTGAACTCGTTCCGCAACGTGCAGCGGGCCATTGAAGTGGAAATAGCCCGCCAGCAGGATTTGCTGGAAGAC
GGCGAAAAAGTGGTTCAGGAAACACGCCTGTACGATGCCGCCCGCAATGTCACCGCATCCATGCGGGGCAAAGAAGAGGC
ACACGACTACCGCTACTTCCCCGATCCTGACCTTATTCCTCTGCATCTGGAAGACGGATGGCTGCAGGAATGGCGCAAGG
CGCTGCCCGAGTTGCCCGCACTCCGCAGAACACGCTTTATGGAGCAGTACGGACTGTCTGTGCAGGACGCCGAACTGCTT
ACCGCCGAACGTGAGCTGGCGGATTTTTATGAGGCTGCGGTGCGGCACGGCGGCGTGCCGAAAAAGGTGGCCAATCTGAT
GATGGGTGAATTCATGCGCGAGCTCAATGAGCGGCGCATCAGCGTGAACGAAGCCGCCATGACTCCCGAAGCCGTGGCCG
AACTGGTACGGCTGCAGGAAGAAGGTGTGGTGAGCAGCAAGATTGTGCACGATATCTTTTCTGACCTGTTCACATCGGGG
TCCATGCCGGAAGCCTATGTGAAGGCCAAGGGACTGGTGCAGATATCTGATTCGGGCGCCATAGAAACCGCAGTGGACGA
GGTGCTGGCTGAAAACCCTGCAGAGGTTGAAGCCTACCGCGGCGGCAAGACCAAACTGATGAGCTTCTTTATGGGGCAGG
TCATGCGCAAGACCAGAGGCAAAGCCAATCCTGCCGTGGTCACCGGATTGCTGACGGAAAAGCTGGGATAG

Upstream 100 bases:

>100_bases
ACCCAGATCTGAATCCGGAACTGTACGGCAACAGAAAAGGAAAGGCATAGCGCTGCGGGGCCGCCCTGCTGGCACATGCT
TTTGTCCAAGGAGTATCTGT

Downstream 100 bases:

>100_bases
CAGGCTGCCGGTATAATGGCGCCGTAATTATTTTATGGAGCAATGCAACAGGCGGTCCCCGGACCGCCTTTTTTTATGCA
TAAGATGCGTCCGCAAAAAG

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MAEYEVVIGLEVHAQLRTSSKLFCACSTEFGSEPNTNVCEICSGMPGVLPVVNRKAVEYAVMMGMAVDCTINNDSVFARK
NYFYPDLPYGYQISQFERPLCEHGHLDIPVNGGTKRIGITRIHMENDAGKNIHSAADNASYVDLNRAGTPLIEIVSEPDM
RSAEEAVAYLKELRSILLYLGICDGNMEEGSFRCDANVSLRPAGQKEFGTRTELKNVNSFRNVQRAIEVEIARQQDLLED
GEKVVQETRLYDAARNVTASMRGKEEAHDYRYFPDPDLIPLHLEDGWLQEWRKALPELPALRRTRFMEQYGLSVQDAELL
TAERELADFYEAAVRHGGVPKKVANLMMGEFMRELNERRISVNEAAMTPEAVAELVRLQEEGVVSSKIVHDIFSDLFTSG
SMPEAYVKAKGLVQISDSGAIETAVDEVLAENPAEVEAYRGGKTKLMSFFMGQVMRKTRGKANPAVVTGLLTEKLG

Sequences:

>Translated_476_residues
MAEYEVVIGLEVHAQLRTSSKLFCACSTEFGSEPNTNVCEICSGMPGVLPVVNRKAVEYAVMMGMAVDCTINNDSVFARK
NYFYPDLPYGYQISQFERPLCEHGHLDIPVNGGTKRIGITRIHMENDAGKNIHSAADNASYVDLNRAGTPLIEIVSEPDM
RSAEEAVAYLKELRSILLYLGICDGNMEEGSFRCDANVSLRPAGQKEFGTRTELKNVNSFRNVQRAIEVEIARQQDLLED
GEKVVQETRLYDAARNVTASMRGKEEAHDYRYFPDPDLIPLHLEDGWLQEWRKALPELPALRRTRFMEQYGLSVQDAELL
TAERELADFYEAAVRHGGVPKKVANLMMGEFMRELNERRISVNEAAMTPEAVAELVRLQEEGVVSSKIVHDIFSDLFTSG
SMPEAYVKAKGLVQISDSGAIETAVDEVLAENPAEVEAYRGGKTKLMSFFMGQVMRKTRGKANPAVVTGLLTEKLG
>Mature_475_residues
AEYEVVIGLEVHAQLRTSSKLFCACSTEFGSEPNTNVCEICSGMPGVLPVVNRKAVEYAVMMGMAVDCTINNDSVFARKN
YFYPDLPYGYQISQFERPLCEHGHLDIPVNGGTKRIGITRIHMENDAGKNIHSAADNASYVDLNRAGTPLIEIVSEPDMR
SAEEAVAYLKELRSILLYLGICDGNMEEGSFRCDANVSLRPAGQKEFGTRTELKNVNSFRNVQRAIEVEIARQQDLLEDG
EKVVQETRLYDAARNVTASMRGKEEAHDYRYFPDPDLIPLHLEDGWLQEWRKALPELPALRRTRFMEQYGLSVQDAELLT
AERELADFYEAAVRHGGVPKKVANLMMGEFMRELNERRISVNEAAMTPEAVAELVRLQEEGVVSSKIVHDIFSDLFTSGS
MPEAYVKAKGLVQISDSGAIETAVDEVLAENPAEVEAYRGGKTKLMSFFMGQVMRKTRGKANPAVVTGLLTEKLG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily

Homologues:

Organism=Homo sapiens, GI4758894, Length=495, Percent_Identity=36.7676767676768, Blast_Score=324, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI115534094, Length=371, Percent_Identity=37.7358490566038, Blast_Score=226, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6319391, Length=510, Percent_Identity=29.2156862745098, Blast_Score=209, Evalue=6e-55,
Organism=Drosophila melanogaster, GI45550808, Length=494, Percent_Identity=34.8178137651822, Blast_Score=282, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GATB_DESDG (Q30Y35)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_389106.1
- ProteinModelPortal:   Q30Y35
- STRING:   Q30Y35
- GeneID:   3757637
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2615
- NMPDR:   fig|207559.3.peg.2802
- eggNOG:   COG0064
- HOGENOM:   HBG395951
- OMA:   LRYLGTC
- ProtClustDB:   PRK05477
- BioCyc:   DDES207559:DDE_2615-MONOMER
- GO:   GO:0006412
- HAMAP:   MF_00121
- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958
- PANTHER:   PTHR11659
- SMART:   SM00845
- TIGRFAMs:   TIGR00133

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey; SSF89095 GatB_Yqey

EC number: 6.3.5.-

Molecular weight: Translated: 53022; Mature: 52891

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEYEVVIGLEVHAQLRTSSKLFCACSTEFGSEPNTNVCEICSGMPGVLPVVNRKAVEYA
CCCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHH
VMMGMAVDCTINNDSVFARKNYFYPDLPYGYQISQFERPLCEHGHLDIPVNGGTKRIGIT
HHHCEEEEEEECCCCEEEECCCCCCCCCCCCCHHHHCCHHHHCCCEEEEECCCCCEEEEE
RIHMENDAGKNIHSAADNASYVDLNRAGTPLIEIVSEPDMRSAEEAVAYLKELRSILLYL
EEEECCCCCCCHHHHCCCCCEEEECCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHH
GICDGNMEEGSFRCDANVSLRPAGQKEFGTRTELKNVNSFRNVQRAIEVEIARQQDLLED
CCCCCCCCCCCEEECCCCEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEKVVQETRLYDAARNVTASMRGKEEAHDYRYFPDPDLIPLHLEDGWLQEWRKALPELPA
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHH
LRRTRFMEQYGLSVQDAELLTAERELADFYEAAVRHGGVPKKVANLMMGEFMRELNERRI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
SVNEAAMTPEAVAELVRLQEEGVVSSKIVHDIFSDLFTSGSMPEAYVKAKGLVQISDSGA
CCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCCC
IETAVDEVLAENPAEVEAYRGGKTKLMSFFMGQVMRKTRGKANPAVVTGLLTEKLG
HHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AEYEVVIGLEVHAQLRTSSKLFCACSTEFGSEPNTNVCEICSGMPGVLPVVNRKAVEYA
CCEEEEEEEEEEEECCCCCEEEEEEECCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHH
VMMGMAVDCTINNDSVFARKNYFYPDLPYGYQISQFERPLCEHGHLDIPVNGGTKRIGIT
HHHCEEEEEEECCCCEEEECCCCCCCCCCCCCHHHHCCHHHHCCCEEEEECCCCCEEEEE
RIHMENDAGKNIHSAADNASYVDLNRAGTPLIEIVSEPDMRSAEEAVAYLKELRSILLYL
EEEECCCCCCCHHHHCCCCCEEEECCCCCCHHEECCCCCCCCHHHHHHHHHHHHHHHHHH
GICDGNMEEGSFRCDANVSLRPAGQKEFGTRTELKNVNSFRNVQRAIEVEIARQQDLLED
CCCCCCCCCCCEEECCCCEECCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GEKVVQETRLYDAARNVTASMRGKEEAHDYRYFPDPDLIPLHLEDGWLQEWRKALPELPA
HHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCHH
LRRTRFMEQYGLSVQDAELLTAERELADFYEAAVRHGGVPKKVANLMMGEFMRELNERRI
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCC
SVNEAAMTPEAVAELVRLQEEGVVSSKIVHDIFSDLFTSGSMPEAYVKAKGLVQISDSGA
CCHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHHHCCCEEEECCCCC
IETAVDEVLAENPAEVEAYRGGKTKLMSFFMGQVMRKTRGKANPAVVTGLLTEKLG
HHHHHHHHHHCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA