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The map label for this gene is moeB [H]

Identifier: 78357502

GI number: 78357502

Start: 2481381

End: 2482235

Strand: Direct

Name: moeB [H]

Synonym: Dde_2459

Alternate gene names: 78357502

Gene position: 2481381-2482235 (Clockwise)

Preceding gene: 78357501

Following gene: 78357504

Centisome position: 66.52

GC content: 61.29

Gene sequence:

>855_bases
ATGACGAGTGCCGATGCCCGTCACGCCGGAGCGCCGCGGGACGACGCTCCTCTGCACACACTTATCATGCAGCACGCTGT
CAACGGCAGACTGCGCATGCGGGCGGTGCAGGGCATAGCGCAGGAATGCGGCTGCCATATCCGCCACGTGGAATGTGTTG
CACTACTTTCCGGAATCCTGCCGGAACGTTACGCGCGCAACACAAAGCAGTTTTCTCTGGAAGACCAGCGGGCGCTGCTG
TTGTCGCGGGTGCTGCTTGTGGGACTGGGGGGACTGGGAGGCCATGTTCTCGATATGCTTGTCAGGCTGGGTGTGGGACA
CATCACGGCAGCGGACGGAGACGTTTTCGAGCCCAGCAATCTGAACAGACAACTGCTCAGTTCCATGTCACGGGTGGGCA
CGTCCAAGGCGCAGGCGGCCAGAGACCACGCCCGCAATACCAACCCCGCAACCGAACTCACGGTTGTCGATCACTACGTG
GATGCTCTTTCGCTGCCGCCGCTGATGCAGCAATGCCATCTGGTCATCGACGCACTGGGCGGACTGCAGTCACGTCTTAT
GCTCCAGAAAGCCGCACAGAACGCCGGAGTGCCGCTGGTAACGGCCGCCGTGGGCGGTTTGACAGGGTATGTGGCCACAG
TATTGCCGGACCAGACAGGCCCCGCCGAACTGCTGGGGTCGGGCGGCACAGGTGAACCGGTGGAAGATACGCTGGGCACT
CCCGCTCCTGTTGTGGCCTGTGCCGCGGCCCTGCAATGCACAGAAGCGGCAAAAATTCTTACCGGCAAACCGCCGTCGCG
CGGTGTACTGTTTTTTGACCTGAACGACAGGACATTTCAGACAGTCATGCTCTGA

Upstream 100 bases:

>100_bases
TGAAAATAATCTTTATAAACGGCGCTCATGTAGAGCCGGAAGCCCTGCTGAACGAAGGTGACAGGCTCGGACTTTTTCCC
GCCGTGGGCGGAGGCTGATC

Downstream 100 bases:

>100_bases
TAAGCTGCACACCGGACACCGGCTGGAATGACTTCTGGCCACACCAGAAACCACGTCACAGTCCGCACGCCTTGCTCCCC
GCTTTTCCCGGCATACCTCA

Product: thiF protein

Products: NA

Alternate protein names: MoaD protein adenylase; Molybdopterin-converting factor subunit 1 adenylase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MTSADARHAGAPRDDAPLHTLIMQHAVNGRLRMRAVQGIAQECGCHIRHVECVALLSGILPERYARNTKQFSLEDQRALL
LSRVLLVGLGGLGGHVLDMLVRLGVGHITAADGDVFEPSNLNRQLLSSMSRVGTSKAQAARDHARNTNPATELTVVDHYV
DALSLPPLMQQCHLVIDALGGLQSRLMLQKAAQNAGVPLVTAAVGGLTGYVATVLPDQTGPAELLGSGGTGEPVEDTLGT
PAPVVACAAALQCTEAAKILTGKPPSRGVLFFDLNDRTFQTVML

Sequences:

>Translated_284_residues
MTSADARHAGAPRDDAPLHTLIMQHAVNGRLRMRAVQGIAQECGCHIRHVECVALLSGILPERYARNTKQFSLEDQRALL
LSRVLLVGLGGLGGHVLDMLVRLGVGHITAADGDVFEPSNLNRQLLSSMSRVGTSKAQAARDHARNTNPATELTVVDHYV
DALSLPPLMQQCHLVIDALGGLQSRLMLQKAAQNAGVPLVTAAVGGLTGYVATVLPDQTGPAELLGSGGTGEPVEDTLGT
PAPVVACAAALQCTEAAKILTGKPPSRGVLFFDLNDRTFQTVML
>Mature_283_residues
TSADARHAGAPRDDAPLHTLIMQHAVNGRLRMRAVQGIAQECGCHIRHVECVALLSGILPERYARNTKQFSLEDQRALLL
SRVLLVGLGGLGGHVLDMLVRLGVGHITAADGDVFEPSNLNRQLLSSMSRVGTSKAQAARDHARNTNPATELTVVDHYVD
ALSLPPLMQQCHLVIDALGGLQSRLMLQKAAQNAGVPLVTAAVGGLTGYVATVLPDQTGPAELLGSGGTGEPVEDTLGTP
APVVACAAALQCTEAAKILTGKPPSRGVLFFDLNDRTFQTVML

Specific function: Catalyzes the adenylation by ATP of the carboxyl group of the C-terminal glycine of sulfur carrier protein moaD [H]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hesA/moeB/thiF family [H]

Homologues:

Organism=Homo sapiens, GI4885649, Length=136, Percent_Identity=35.2941176470588, Blast_Score=74, Evalue=1e-13,
Organism=Escherichia coli, GI1787048, Length=229, Percent_Identity=35.3711790393013, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87082356, Length=227, Percent_Identity=28.6343612334802, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1789177, Length=158, Percent_Identity=27.8481012658228, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321903, Length=167, Percent_Identity=30.5389221556886, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320598, Length=144, Percent_Identity=28.4722222222222, Blast_Score=73, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6325323, Length=151, Percent_Identity=29.8013245033113, Blast_Score=70, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24660640, Length=143, Percent_Identity=34.965034965035, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582879, Length=213, Percent_Identity=29.5774647887324, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR012730
- InterPro:   IPR016040
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 29909; Mature: 29778

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSADARHAGAPRDDAPLHTLIMQHAVNGRLRMRAVQGIAQECGCHIRHVECVALLSGIL
CCCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
PERYARNTKQFSLEDQRALLLSRVLLVGLGGLGGHVLDMLVRLGVGHITAADGDVFEPSN
HHHHHCCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCC
LNRQLLSSMSRVGTSKAQAARDHARNTNPATELTVVDHYVDALSLPPLMQQCHLVIDALG
CCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GLQSRLMLQKAAQNAGVPLVTAAVGGLTGYVATVLPDQTGPAELLGSGGTGEPVEDTLGT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHCCC
PAPVVACAAALQCTEAAKILTGKPPSRGVLFFDLNDRTFQTVML
CHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHCCC
>Mature Secondary Structure 
TSADARHAGAPRDDAPLHTLIMQHAVNGRLRMRAVQGIAQECGCHIRHVECVALLSGIL
CCCCCCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
PERYARNTKQFSLEDQRALLLSRVLLVGLGGLGGHVLDMLVRLGVGHITAADGDVFEPSN
HHHHHCCCHHCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCEEECCCCCCCCCC
LNRQLLSSMSRVGTSKAQAARDHARNTNPATELTVVDHYVDALSLPPLMQQCHLVIDALG
CCHHHHHHHHHHCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
GLQSRLMLQKAAQNAGVPLVTAAVGGLTGYVATVLPDQTGPAELLGSGGTGEPVEDTLGT
HHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCHHHCCC
PAPVVACAAALQCTEAAKILTGKPPSRGVLFFDLNDRTFQTVML
CHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3045084; 8905232; 9278503 [H]