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The map label for this gene is rpfG [H]

Identifier: 78357500

GI number: 78357500

Start: 2480081

End: 2481157

Strand: Direct

Name: rpfG [H]

Synonym: Dde_2457

Alternate gene names: 78357500

Gene position: 2480081-2481157 (Clockwise)

Preceding gene: 78357499

Following gene: 78357501

Centisome position: 66.49

GC content: 58.77

Gene sequence:

>1077_bases
ATGACCACAGAATCCATTGCCGTTCTTGTGATCGATGACGATCCCGCCATTCTTCAGAATATCGTCTGCTATCTGGAAGA
CTCTGATTTTATCTGCTTCGGAGCCGCCGACGGTGCCGCCGGTATCGAAACATTTGAAAGAGAAAGGCCTGACGCCGTCA
TTGTGGACCTGAACATGCCCGGCATGAACGGACGCGATGTCATCGCACGCATCAACGCACAATCGCCCACCACGCCGGTC
ATCGTGGTTTCCGGCACAGGTGTCATTGATGAAGCGGTCGAATCCATGCGTGTCGGCGCGTGGGACTTTCTTTCAAAGCC
CGTGCGCCGCATGGAAGAGCTGGAAAACGCGGTGCGCGCCGGACTGCGCCGTGCCCGCACCATGCGCGAAAGCAATGAAT
ACAAAGAAACGCTGGAGCGCATGGTTAAACGACGCACCCGCGAACTGCAGCTGGCAAACGACCGGACCGAAGCCATTCTG
CTTGCCACCGTGCAGTCGCTCAGCACCGTCATCAACCTGAAAGACGCCTATACCGGCACCCACCAGCAGCGTGTGGCGCT
GATAGCCCCGGCCATTGCCGAAGAACTGGGCATGGAACAACGCGCGGTGGAAGCCGTGCGGCTGGCGGCACTGCTGCACG
ATGTGGGAAAAATGGGACTGCCGTCGGAATTTCTTACCAAACCATCGCGGCTGGACGCCAACGAGATGCAGTTTATCCGC
CAGCACCCGCAGCTGGGGTATGATATCCTCAAAGACATTCCCTTTGAACTGCCCATACCCGAAATGGTTCTGCAGCACCA
TGAACGGCTGGACGGCTCGGGGTATCCGCGCGGGCTTGCCGGCGACGACATACTGCCCGAGGCACGCATTCTGGCGGTTG
CCGACACCGTGGAAGCCATCTGTTCGCACAGACCATGGCGTCCGGCCCATCCGTTCAGCCATGCGCTGCACGAGCTGACA
GCGGGTAAAAACACGCTGTATTGCCCCGATGCAGTGAACAGCTGCATTGACCTGATAACACGGGGCGACCCCCGCCTGAC
AACTTTCACCGCAACCATCAAGGGACTGCCGTCATGA

Upstream 100 bases:

>100_bases
TCCGCGCTGTTTTAGCCTGACCTCCCTGTCCCGTATTGCGTTATCAGGCAGGCGCATATACCATATTTTCAATTCTGCAC
CCCTAGCCTGCGACGGACCC

Downstream 100 bases:

>100_bases
AGATAGAAGTGAAATGCTTTGCCACGCTGGCCCCGCATACCCCGGCGGAGGGCACCATGGACGTGCCGCCGCAGACATCT
GTAAACGACATCATGCGCCA

Product: metal dependent phosphohydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 358; Mature: 357

Protein sequence:

>358_residues
MTTESIAVLVIDDDPAILQNIVCYLEDSDFICFGAADGAAGIETFERERPDAVIVDLNMPGMNGRDVIARINAQSPTTPV
IVVSGTGVIDEAVESMRVGAWDFLSKPVRRMEELENAVRAGLRRARTMRESNEYKETLERMVKRRTRELQLANDRTEAIL
LATVQSLSTVINLKDAYTGTHQQRVALIAPAIAEELGMEQRAVEAVRLAALLHDVGKMGLPSEFLTKPSRLDANEMQFIR
QHPQLGYDILKDIPFELPIPEMVLQHHERLDGSGYPRGLAGDDILPEARILAVADTVEAICSHRPWRPAHPFSHALHELT
AGKNTLYCPDAVNSCIDLITRGDPRLTTFTATIKGLPS

Sequences:

>Translated_358_residues
MTTESIAVLVIDDDPAILQNIVCYLEDSDFICFGAADGAAGIETFERERPDAVIVDLNMPGMNGRDVIARINAQSPTTPV
IVVSGTGVIDEAVESMRVGAWDFLSKPVRRMEELENAVRAGLRRARTMRESNEYKETLERMVKRRTRELQLANDRTEAIL
LATVQSLSTVINLKDAYTGTHQQRVALIAPAIAEELGMEQRAVEAVRLAALLHDVGKMGLPSEFLTKPSRLDANEMQFIR
QHPQLGYDILKDIPFELPIPEMVLQHHERLDGSGYPRGLAGDDILPEARILAVADTVEAICSHRPWRPAHPFSHALHELT
AGKNTLYCPDAVNSCIDLITRGDPRLTTFTATIKGLPS
>Mature_357_residues
TTESIAVLVIDDDPAILQNIVCYLEDSDFICFGAADGAAGIETFERERPDAVIVDLNMPGMNGRDVIARINAQSPTTPVI
VVSGTGVIDEAVESMRVGAWDFLSKPVRRMEELENAVRAGLRRARTMRESNEYKETLERMVKRRTRELQLANDRTEAILL
ATVQSLSTVINLKDAYTGTHQQRVALIAPAIAEELGMEQRAVEAVRLAALLHDVGKMGLPSEFLTKPSRLDANEMQFIRQ
HPQLGYDILKDIPFELPIPEMVLQHHERLDGSGYPRGLAGDDILPEARILAVADTVEAICSHRPWRPAHPFSHALHELTA
GKNTLYCPDAVNSCIDLITRGDPRLTTFTATIKGLPS

Specific function: Positively regulates the synthesis of extracellular enzymes and extracellular polysaccharides. The cell-cell signaling system encoded by rpf genes is required for full virulence and is implicated in regulation of the formation and dispersal of biofilms [H

COG id: COG3437

COG function: function code KT; Response regulator containing a CheY-like receiver domain and an HD-GYP domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788905, Length=110, Percent_Identity=34.5454545454545, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1790299, Length=100, Percent_Identity=33, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790552, Length=126, Percent_Identity=27.7777777777778, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1789402, Length=105, Percent_Identity=34.2857142857143, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1788550, Length=131, Percent_Identity=25.9541984732824, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR003607
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 39580; Mature: 39449

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTESIAVLVIDDDPAILQNIVCYLEDSDFICFGAADGAAGIETFERERPDAVIVDLNMP
CCCCCEEEEEECCCHHHHHHHHHEEECCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCC
GMNGRDVIARINAQSPTTPVIVVSGTGVIDEAVESMRVGAWDFLSKPVRRMEELENAVRA
CCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GLRRARTMRESNEYKETLERMVKRRTRELQLANDRTEAILLATVQSLSTVINLKDAYTGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
HQQRVALIAPAIAEELGMEQRAVEAVRLAALLHDVGKMGLPSEFLTKPSRLDANEMQFIR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHH
QHPQLGYDILKDIPFELPIPEMVLQHHERLDGSGYPRGLAGDDILPEARILAVADTVEAI
HCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CSHRPWRPAHPFSHALHELTAGKNTLYCPDAVNSCIDLITRGDPRLTTFTATIKGLPS
HCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCCEEEEEEEECCCCC
>Mature Secondary Structure 
TTESIAVLVIDDDPAILQNIVCYLEDSDFICFGAADGAAGIETFERERPDAVIVDLNMP
CCCCEEEEEECCCHHHHHHHHHEEECCCEEEEECCCCCHHHHHHHHCCCCEEEEEECCC
GMNGRDVIARINAQSPTTPVIVVSGTGVIDEAVESMRVGAWDFLSKPVRRMEELENAVRA
CCCCCCEEEEECCCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
GLRRARTMRESNEYKETLERMVKRRTRELQLANDRTEAILLATVQSLSTVINLKDAYTGT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
HQQRVALIAPAIAEELGMEQRAVEAVRLAALLHDVGKMGLPSEFLTKPSRLDANEMQFIR
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCHHHHHHHH
QHPQLGYDILKDIPFELPIPEMVLQHHERLDGSGYPRGLAGDDILPEARILAVADTVEAI
HCCCCCHHHHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
CSHRPWRPAHPFSHALHELTAGKNTLYCPDAVNSCIDLITRGDPRLTTFTATIKGLPS
HCCCCCCCCCHHHHHHHHHHCCCCEEECHHHHHHHHHHHHCCCCCEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11123673 [H]