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The map label for this gene is ruvA

Identifier: 78357366

GI number: 78357366

Start: 2340745

End: 2341350

Strand: Reverse

Name: ruvA

Synonym: Dde_2323

Alternate gene names: 78357366

Gene position: 2341350-2340745 (Counterclockwise)

Preceding gene: 78357367

Following gene: 78357365

Centisome position: 62.77

GC content: 58.09

Gene sequence:

>606_bases
ATGATTGCATATATTGAAGGGCGAGTGGCCGAAGTGACCGAGCAGAGCTGTGTGGTGGTTACGCAGGGCGGGGTAGGGTA
CGAAGTGCATCTGCCTTCGCATCTGCTTGCGCGGCTGCCTGAACAGGGCGCGGCGGTTTCTTTTTACATCCACACGGTGG
TGCGCGAAGACGCGCTGGAACTGTACGGATTTTCCGGATGGGATGAGCGGCAGACGTTTCTTGTGCTTATTTCCATTTCC
AAAGTGGGGGCCAAGACGGCTCTTGCCGTGCTTTCTCTGTACCGGCCCGACGACCTGCGGCGCATTGTTGCCGACGATGA
TGTGACGGCGCTTACGCGGGTTTCCGGCATCGGCAAAAAAAGCGCCCAGCACATTCTGCTGGAACTGAAATACAAGCTTA
AAGTGGAATCGCTGCCTGCTTCGGCCGGTCTGGCCGCCGGTGTGCCGGGCAGTGTGCTGCGCGATGCCGTTCAGGCACTG
GGCAATCTCGGTTATGCCGAAGAAGAAGCCGCCCCCGTGCTGAAAAACATTCTGAAGCAGGATCCCGATCTGGATGTGAG
CGAAGCGTTGCGTGCGGCGCTTAAAGCGCTTGCAAAGGCGAGATGA

Upstream 100 bases:

>100_bases
GGTAACTTCTGCCTGCCGCTGTGAACCCGGCGCGCAATGCGGACAGCGGCCTTTTATTCTCTGTTCCGTTGATGTACAAC
CCTACTTCAGGAAATCTGCC

Downstream 100 bases:

>100_bases
TGATGGAACAGGAATGTGTTGATGATTCGATCCGCCCGAGAACGCTGGATGATTTTATCGGGCAGGAAGACCTGCGCGCC
AACCTGCGGGTGTATCTGCA

Product: Holliday junction DNA helicase RuvA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 201; Mature: 201

Protein sequence:

>201_residues
MIAYIEGRVAEVTEQSCVVVTQGGVGYEVHLPSHLLARLPEQGAAVSFYIHTVVREDALELYGFSGWDERQTFLVLISIS
KVGAKTALAVLSLYRPDDLRRIVADDDVTALTRVSGIGKKSAQHILLELKYKLKVESLPASAGLAAGVPGSVLRDAVQAL
GNLGYAEEEAAPVLKNILKQDPDLDVSEALRAALKALAKAR

Sequences:

>Translated_201_residues
MIAYIEGRVAEVTEQSCVVVTQGGVGYEVHLPSHLLARLPEQGAAVSFYIHTVVREDALELYGFSGWDERQTFLVLISIS
KVGAKTALAVLSLYRPDDLRRIVADDDVTALTRVSGIGKKSAQHILLELKYKLKVESLPASAGLAAGVPGSVLRDAVQAL
GNLGYAEEEAAPVLKNILKQDPDLDVSEALRAALKALAKAR
>Mature_201_residues
MIAYIEGRVAEVTEQSCVVVTQGGVGYEVHLPSHLLARLPEQGAAVSFYIHTVVREDALELYGFSGWDERQTFLVLISIS
KVGAKTALAVLSLYRPDDLRRIVADDDVTALTRVSGIGKKSAQHILLELKYKLKVESLPASAGLAAGVPGSVLRDAVQAL
GNLGYAEEEAAPVLKNILKQDPDLDVSEALRAALKALAKAR

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG0632

COG function: function code L; Holliday junction resolvasome, DNA-binding subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvA family

Homologues:

Organism=Escherichia coli, GI1788168, Length=204, Percent_Identity=38.2352941176471, Blast_Score=113, Evalue=9e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVA_DESDG (Q30YX6)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388815.1
- ProteinModelPortal:   Q30YX6
- SMR:   Q30YX6
- STRING:   Q30YX6
- GeneID:   3757334
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2323
- NMPDR:   fig|207559.3.peg.618
- eggNOG:   COG0632
- HOGENOM:   HBG635309
- OMA:   LSIETYV
- ProtClustDB:   PRK14602
- BioCyc:   DDES207559:DDE_2323-MONOMER
- HAMAP:   MF_00031
- InterPro:   IPR011114
- InterPro:   IPR013849
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR000085
- InterPro:   IPR010994
- Gene3D:   G3DSA:2.40.50.140
- SMART:   SM00278
- TIGRFAMs:   TIGR00084

Pfam domain/function: PF07499 RuvA_C; PF01330 RuvA_N; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like; SSF46929 RuvA_C-like

EC number: =3.6.4.12

Molecular weight: Translated: 21560; Mature: 21560

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIAYIEGRVAEVTEQSCVVVTQGGVGYEVHLPSHLLARLPEQGAAVSFYIHTVVREDALE
CEEEECCCHHHHCCCCEEEEEECCCCEEEECHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
LYGFSGWDERQTFLVLISISKVGAKTALAVLSLYRPDDLRRIVADDDVTALTRVSGIGKK
HCCCCCCCCCCEEEEEEEECHHCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCHH
SAQHILLELKYKLKVESLPASAGLAAGVPGSVLRDAVQALGNLGYAEEEAAPVLKNILKQ
HHHHHHHEEHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
DPDLDVSEALRAALKALAKAR
CCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIAYIEGRVAEVTEQSCVVVTQGGVGYEVHLPSHLLARLPEQGAAVSFYIHTVVREDALE
CEEEECCCHHHHCCCCEEEEEECCCCEEEECHHHHHHHCCCCCCEEEHHHHHHHHHHHHH
LYGFSGWDERQTFLVLISISKVGAKTALAVLSLYRPDDLRRIVADDDVTALTRVSGIGKK
HCCCCCCCCCCEEEEEEEECHHCHHHHHHHHHHCCCHHHHHHHCCCCHHHHHHHHCCCHH
SAQHILLELKYKLKVESLPASAGLAAGVPGSVLRDAVQALGNLGYAEEEAAPVLKNILKQ
HHHHHHHEEHEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHC
DPDLDVSEALRAALKALAKAR
CCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA