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The map label for this gene is nrgA [H]

Identifier: 78357354

GI number: 78357354

Start: 2328899

End: 2330104

Strand: Reverse

Name: nrgA [H]

Synonym: Dde_2311

Alternate gene names: 78357354

Gene position: 2330104-2328899 (Counterclockwise)

Preceding gene: 78357358

Following gene: 78357353

Centisome position: 62.47

GC content: 59.29

Gene sequence:

>1206_bases
ATGAACGCGGCAGATACCGCTTTCATTCTTGTCTGTTCGGCTCTGGTGATGTTTATGACACCGGGGCTGGCGTTGTTTTA
CGGGGGGCTTGTCAGATCGCGCAATGTGCTCAGCACCACCATGCACAGCGTGGCTTTGCTCGGCATTGCCTCACTTGTCT
GGTGGGCAGTGGGGTATTCTCTGGCGTTCGGCAGTGACCACGGGGGCATTATCGGCGGGCTGGAGTATCTTTTTCTGCAT
AACGTAGGCACGGAACCGGGTCCTGTCGATAATCTGCCGCATCTGCTGTTCATGGTTTTTCAGTGCATGTTTGCCGTTAT
CACACCGGCGTTGATTTCGGGTGCCTATGCCGAGCGTATCCGTTTCGGGGCGATGGTGCTGTTTTCTGTTTTATGGCTTG
TGCTGGTGTATGCGCCCATGGCGCACTGGGTTTGGGGCGGCGGATGGATGGCCCGGCTGGGTGCGCTTGATTTTGCCGGG
GGGGCCGTGGTGCATATGAGCTCTGCCGCTGCTGCGCTGGCGGCTGCCAATGTGCTGGGACTGCGCCGCGGATACGGGCG
CGAGGCATTTATTCCGCACAATCTGCCCATGACAGTACTGGGAGCAGGTATTCTGTGGTTCGGCTGGTTCGGTTTTAACG
CCGGAAGCGCGCTGGCGGCCAACGGCGCGGCAGCCAATGCATTTGTAACCACACATCTTGCCGCGGCTGCGGCTCTGGTG
GGCTGGCTTGCCGTGGAAAAACTGCATGGCGGCAGAGCAACCACCCTTGGTGCGGCTTCGGGAGCCGTGGCGGGACTGGT
TGCCATTACTCCCGCCGCAGGGTTTGTCACACCGGCTTCAGCGGTGTTCATCGGGCTGGCCGGCGGAGTGCTGTGTTACG
GCGGTGTGTTTCTCAAGCACAAGCTGCGTTATGATGACGCTCTTGATGTGGTGGGGATTCATGGTGTTGGGGGCACGTGG
GGCGCGCTGGCAACCGGAATTTTTGCCGATGCCGCCGTGGGCGGGGCAGACGGTCTGCTGCACGGCAACGCATATCAGCT
CTGGGTGCAGTTTGTCTCGGTTGTGGCTACATGGCTGTTTTGCTATGTTGCCAGCTGGTTGCTTTTCAAGGCTGTCGATG
CGGTCATGGGACTCAGGGTCTCCGGTGAAGCCGAGCTTGCCGGTCTTGATGTGAGTGAACACAACGAAACCGCCTACCAG
ATGTAA

Upstream 100 bases:

>100_bases
GGCAATGATTGCGAAAAAAACTTCTTCTGGCCTGCTGCTTGCTTTCTTCCGGCTACCGGCAGGGGATTGCAATTTTTTGT
TTTTACTTCAGGAGGAGTCT

Downstream 100 bases:

>100_bases
AGGCAGGGACTCAGTATGAAAAAAGTTGAAATCATCATCAGGCCCTTCAAGCTGGATGAGGTGAAAGAAAGTCTTACCGA
GCTTGATATCAAAGGCATGA

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Membrane protein NrgA; Protein AmtB [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MNAADTAFILVCSALVMFMTPGLALFYGGLVRSRNVLSTTMHSVALLGIASLVWWAVGYSLAFGSDHGGIIGGLEYLFLH
NVGTEPGPVDNLPHLLFMVFQCMFAVITPALISGAYAERIRFGAMVLFSVLWLVLVYAPMAHWVWGGGWMARLGALDFAG
GAVVHMSSAAAALAAANVLGLRRGYGREAFIPHNLPMTVLGAGILWFGWFGFNAGSALAANGAAANAFVTTHLAAAAALV
GWLAVEKLHGGRATTLGAASGAVAGLVAITPAAGFVTPASAVFIGLAGGVLCYGGVFLKHKLRYDDALDVVGIHGVGGTW
GALATGIFADAAVGGADGLLHGNAYQLWVQFVSVVATWLFCYVASWLLFKAVDAVMGLRVSGEAELAGLDVSEHNETAYQ
M

Sequences:

>Translated_401_residues
MNAADTAFILVCSALVMFMTPGLALFYGGLVRSRNVLSTTMHSVALLGIASLVWWAVGYSLAFGSDHGGIIGGLEYLFLH
NVGTEPGPVDNLPHLLFMVFQCMFAVITPALISGAYAERIRFGAMVLFSVLWLVLVYAPMAHWVWGGGWMARLGALDFAG
GAVVHMSSAAAALAAANVLGLRRGYGREAFIPHNLPMTVLGAGILWFGWFGFNAGSALAANGAAANAFVTTHLAAAAALV
GWLAVEKLHGGRATTLGAASGAVAGLVAITPAAGFVTPASAVFIGLAGGVLCYGGVFLKHKLRYDDALDVVGIHGVGGTW
GALATGIFADAAVGGADGLLHGNAYQLWVQFVSVVATWLFCYVASWLLFKAVDAVMGLRVSGEAELAGLDVSEHNETAYQ
M
>Mature_401_residues
MNAADTAFILVCSALVMFMTPGLALFYGGLVRSRNVLSTTMHSVALLGIASLVWWAVGYSLAFGSDHGGIIGGLEYLFLH
NVGTEPGPVDNLPHLLFMVFQCMFAVITPALISGAYAERIRFGAMVLFSVLWLVLVYAPMAHWVWGGGWMARLGALDFAG
GAVVHMSSAAAALAAANVLGLRRGYGREAFIPHNLPMTVLGAGILWFGWFGFNAGSALAANGAAANAFVTTHLAAAAALV
GWLAVEKLHGGRATTLGAASGAVAGLVAITPAAGFVTPASAVFIGLAGGVLCYGGVFLKHKLRYDDALDVVGIHGVGGTW
GALATGIFADAAVGGADGLLHGNAYQLWVQFVSVVATWLFCYVASWLLFKAVDAVMGLRVSGEAELAGLDVSEHNETAYQ
M

Specific function: Functions as an ammonium and methylammonium transporter in the absence of glutamine. Required for ammonium utilization at low concentrations or at low pH values, when ammonium is the single nitrogen source. Required for binding of NrgB to the membrane [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=404, Percent_Identity=47.5247524752475, Blast_Score=331, Evalue=4e-92,
Organism=Caenorhabditis elegans, GI17550182, Length=435, Percent_Identity=30.8045977011494, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17550180, Length=431, Percent_Identity=28.7703016241299, Blast_Score=139, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI32564042, Length=418, Percent_Identity=27.7511961722488, Blast_Score=121, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI133931100, Length=421, Percent_Identity=27.790973871734, Blast_Score=116, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324187, Length=418, Percent_Identity=38.9952153110048, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6325396, Length=411, Percent_Identity=33.8199513381995, Blast_Score=221, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6321559, Length=412, Percent_Identity=33.0097087378641, Blast_Score=207, Evalue=2e-54,
Organism=Drosophila melanogaster, GI161078314, Length=422, Percent_Identity=31.042654028436, Blast_Score=151, Evalue=9e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 41595; Mature: 41595

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00639 THIOL_PROTEASE_HIS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNAADTAFILVCSALVMFMTPGLALFYGGLVRSRNVLSTTMHSVALLGIASLVWWAVGYS
CCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHH
LAFGSDHGGIIGGLEYLFLHNVGTEPGPVDNLPHLLFMVFQCMFAVITPALISGAYAERI
HEECCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RFGAMVLFSVLWLVLVYAPMAHWVWGGGWMARLGALDFAGGAVVHMSSAAAALAAANVLG
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCCCEEEEHHHHHHHHHHHHHH
LRRGYGREAFIPHNLPMTVLGAGILWFGWFGFNAGSALAANGAAANAFVTTHLAAAAALV
HHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHH
GWLAVEKLHGGRATTLGAASGAVAGLVAITPAAGFVTPASAVFIGLAGGVLCYGGVFLKH
HHHHHHHHCCCCCEECCCCCHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KLRYDDALDVVGIHGVGGTWGALATGIFADAAVGGADGLLHGNAYQLWVQFVSVVATWLF
HHCCCCCCCEEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHH
CYVASWLLFKAVDAVMGLRVSGEAELAGLDVSEHNETAYQM
HHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8282685; 9353933; 9384377 [H]