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The map label for this gene is hmeA [H]

Identifier: 78357317

GI number: 78357317

Start: 2294518

End: 2295294

Strand: Direct

Name: hmeA [H]

Synonym: Dde_2274

Alternate gene names: 78357317

Gene position: 2294518-2295294 (Clockwise)

Preceding gene: 78357316

Following gene: 78357318

Centisome position: 61.51

GC content: 56.89

Gene sequence:

>777_bases
ATGTTCGACAGAAGAAGATTCCTCAAGATTGCTGGTCTGTCGGCCCTCGGTCTTGGCGCTGGTTCCGCGATTGGAGTGGC
AGAAGCGCTGGCTGCGCCTAAAAAGGATCCTTACAGCGCCAATGCCCATGCGCTGCATGCCGGTCGCTGGGCCATGGTTA
TCGATACCCGCAAGTTCGAGACTCCCGAAGACTTCCAGAAGCTCATCACGGCCTGCCACAAGCAGCACAATGTGCCCGAC
ATTCCCAATGATCAGCACATTATGTGGCTGTGGACGGACAAGTACGAGCACACCTTCCCCGAACAGGCTAACAACCATCT
GTCCGATGAAGTGAAGGAGCGGGACTTTCTGCTGCTGTGCAACCACTGCGAAAACCCGCCGTGCGTCCGTGTGTGCCCCA
CAAAGGCCACTTACAAACGCCCCGACGGCATTGTTGCCATGGACTACCACCGCTGCATCGGCTGCCGGTTCTGCATGGCA
GGCTGCCCCTACGGCGCGCGTAGCTTCAACTACGGCAACCCCAGAGAATACCTGAAGGATATCACCAACCCCGCTTTCCC
CACACGTATGCGCGGCGTTGTTGAAAAATGCAACTTCTGCGTCGAACGTCTGGCTCAGGGGCTGATGCCCTCCTGCGTTG
AAGCGTCTGAAGGTGCCATTGTCTTTGGCGATCTGAACGATCCGGATTCCGAAGTGCGTCAGGTTCTGGCCGAAAACTTC
ACCATCCGCCGTAAACCATCTCTGGGCACGCAGCCCGGTGTATACTACATCATCTAA

Upstream 100 bases:

>100_bases
GCCACACCAACAAGGCCGAATTCTGCGATAAGTGTCACGAAACCAACAGTGTCGATCCCTACTGCTGGGATTGCCACGTA
GCCCCCAGGGGGAACCAGTA

Downstream 100 bases:

>100_bases
GGGGAGATGCCATGATCGAAAAAGCGCTCAAAGGCTCTCCGGGTTATTACATCTGGCTGGCCTTCCTCGGTTCACTTATC
GGCCTCGGCTTGTTTACGTA

Product: reductase, iron-sulfur binding subunit

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
MFDRRRFLKIAGLSALGLGAGSAIGVAEALAAPKKDPYSANAHALHAGRWAMVIDTRKFETPEDFQKLITACHKQHNVPD
IPNDQHIMWLWTDKYEHTFPEQANNHLSDEVKERDFLLLCNHCENPPCVRVCPTKATYKRPDGIVAMDYHRCIGCRFCMA
GCPYGARSFNYGNPREYLKDITNPAFPTRMRGVVEKCNFCVERLAQGLMPSCVEASEGAIVFGDLNDPDSEVRQVLAENF
TIRRKPSLGTQPGVYYII

Sequences:

>Translated_258_residues
MFDRRRFLKIAGLSALGLGAGSAIGVAEALAAPKKDPYSANAHALHAGRWAMVIDTRKFETPEDFQKLITACHKQHNVPD
IPNDQHIMWLWTDKYEHTFPEQANNHLSDEVKERDFLLLCNHCENPPCVRVCPTKATYKRPDGIVAMDYHRCIGCRFCMA
GCPYGARSFNYGNPREYLKDITNPAFPTRMRGVVEKCNFCVERLAQGLMPSCVEASEGAIVFGDLNDPDSEVRQVLAENF
TIRRKPSLGTQPGVYYII
>Mature_258_residues
MFDRRRFLKIAGLSALGLGAGSAIGVAEALAAPKKDPYSANAHALHAGRWAMVIDTRKFETPEDFQKLITACHKQHNVPD
IPNDQHIMWLWTDKYEHTFPEQANNHLSDEVKERDFLLLCNHCENPPCVRVCPTKATYKRPDGIVAMDYHRCIGCRFCMA
GCPYGARSFNYGNPREYLKDITNPAFPTRMRGVVEKCNFCVERLAQGLMPSCVEASEGAIVFGDLNDPDSEVRQVLAENF
TIRRKPSLGTQPGVYYII

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=260, Percent_Identity=30.7692307692308, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI226510944, Length=259, Percent_Identity=29.7297297297297, Blast_Score=89, Evalue=2e-19,
Organism=Escherichia coli, GI1789370, Length=247, Percent_Identity=29.5546558704453, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1787122, Length=111, Percent_Identity=38.7387387387387, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1787872, Length=118, Percent_Identity=38.9830508474576, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1787478, Length=163, Percent_Identity=33.1288343558282, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787740, Length=105, Percent_Identity=39.0476190476191, Blast_Score=74, Evalue=7e-15,
Organism=Escherichia coli, GI1787749, Length=106, Percent_Identity=35.8490566037736, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790326, Length=121, Percent_Identity=32.2314049586777, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29038; Mature: 29038

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
4.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
7.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFDRRRFLKIAGLSALGLGAGSAIGVAEALAAPKKDPYSANAHALHAGRWAMVIDTRKFE
CCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCC
TPEDFQKLITACHKQHNVPDIPNDQHIMWLWTDKYEHTFPEQANNHLSDEVKERDFLLLC
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEE
NHCENPPCVRVCPTKATYKRPDGIVAMDYHRCIGCRFCMAGCPYGARSFNYGNPREYLKD
CCCCCCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
ITNPAFPTRMRGVVEKCNFCVERLAQGLMPSCVEASEGAIVFGDLNDPDSEVRQVLAENF
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHCC
TIRRKPSLGTQPGVYYII
EEEECCCCCCCCCEEEEC
>Mature Secondary Structure
MFDRRRFLKIAGLSALGLGAGSAIGVAEALAAPKKDPYSANAHALHAGRWAMVIDTRKFE
CCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHCCCCCCCCCCCEEEECCCEEEEEECCCCC
TPEDFQKLITACHKQHNVPDIPNDQHIMWLWTDKYEHTFPEQANNHLSDEVKERDFLLLC
CHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCEEEEE
NHCENPPCVRVCPTKATYKRPDGIVAMDYHRCIGCRFCMAGCPYGARSFNYGNPREYLKD
CCCCCCCEEEECCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHH
ITNPAFPTRMRGVVEKCNFCVERLAQGLMPSCVEASEGAIVFGDLNDPDSEVRQVLAENF
HCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEECCCCCCHHHHHHHHHHCC
TIRRKPSLGTQPGVYYII
EEEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]