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The map label for this gene is rpoA

Identifier: 78357274

GI number: 78357274

Start: 2269114

End: 2270160

Strand: Reverse

Name: rpoA

Synonym: Dde_2231

Alternate gene names: 78357274

Gene position: 2270160-2269114 (Counterclockwise)

Preceding gene: 78357275

Following gene: 78357273

Centisome position: 60.86

GC content: 53.77

Gene sequence:

>1047_bases
ATGCTCATCAAACAAGGCGACAGGCTTATCAACACGCGTAACTGGTCCGAGCTGGTCAAGCCCGATCAAATCTCCCGGGC
TAGTGAATCCGCCGACACCATGTACGGCAAGTTCGTCTGCGAACCTCTGGAGCGCGGCTACGGAACGACCATCGGCAATG
CCATGCGTCGCGTCCTGCTGGCGTCCCTGCAGGGCGCCGCGTTTGTGGCCGTAAAGATCTCTGGAGTTCAGCACGAGTTC
ACCACCATTCCCGGTGTGCTTGAAGACGTTACCGATATCGTACTCAACCTTAAACAGGTCCGTCTTGCCATGGATACCGA
GGAGCCTCAGCACCTTGAGCTCCATGTCAACAAGAGCGGCGAAGTTAAGGCCGGCGACATCAAGTGCAATCAGCACGTGA
TGGTTCTGAATGCAGATCAGCATCTTATGACGCTGACTGAGGACGTTGAGCTGACCTTCGAACTCGAAGTTCGTATGGGT
AAGGGCTATGTTCCTGCCGATATGCACGAAGGGCTGAGCGACGAGATCGGCCTTATTGCCCTTGATGCCAGCTTTTCGCC
GGTACGCAAGGTCGCCTACACGGTGGAGCAGGCTCGCGTTGGCCAGATGACCAACTACGACAAGCTCATCCTTGAAGTGT
GGACCGACGGCTCCATATCCCCCGAGGATGCGATTGCGTACAGTGCCAAGATCATCAAGGATCAGATTTCGGTCTTCATC
AACTTTGACGAGCGTATTTCCGAACAGGAAAACGAAGGCAGAAGCTCTGCTTCTGACGTCAATGAAAACCTGTTCAAGGG
CATTGACGAGCTCGAACTTTCTGTTCGCGCCACCAACTGCCTGAAGTCGGCCAACATTTCGCTGGTCGGCGAGCTGGTGC
AGAAGTCCGAAGGTGAAATGCTGAAAACCAAGAACTTTGGCCGTAAGTCGCTTGACGAAATCCGTCGGGTACTGGGCGAA
ATGTCTCTCGATTTCGGCATGAAGGTCGATGGCTTCGAGAAGAAATATCAGGAATGGTTGAAGAGGAAGCAGCAAAATGA
GGCATAG

Upstream 100 bases:

>100_bases
CCGTGGTGTAGTCAAGGCCCTGCCGCAGCGCGAGGACATCCAGTTCCCGATGAGCGAACAGCTTATCGTCGAACTTTACT
CCAAATAAGCAGGTTCGAGC

Downstream 100 bases:

>100_bases
AAAATCCGGCAGAAAGCTGAACAGGAGTTCCTCGCATCGGTCTGCTCTGTTCCGCAACATGGCCAAATCTCTTTTGACGT
ACGGTAAAATCCGCACGACC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MLIKQGDRLINTRNWSELVKPDQISRASESADTMYGKFVCEPLERGYGTTIGNAMRRVLLASLQGAAFVAVKISGVQHEF
TTIPGVLEDVTDIVLNLKQVRLAMDTEEPQHLELHVNKSGEVKAGDIKCNQHVMVLNADQHLMTLTEDVELTFELEVRMG
KGYVPADMHEGLSDEIGLIALDASFSPVRKVAYTVEQARVGQMTNYDKLILEVWTDGSISPEDAIAYSAKIIKDQISVFI
NFDERISEQENEGRSSASDVNENLFKGIDELELSVRATNCLKSANISLVGELVQKSEGEMLKTKNFGRKSLDEIRRVLGE
MSLDFGMKVDGFEKKYQEWLKRKQQNEA

Sequences:

>Translated_348_residues
MLIKQGDRLINTRNWSELVKPDQISRASESADTMYGKFVCEPLERGYGTTIGNAMRRVLLASLQGAAFVAVKISGVQHEF
TTIPGVLEDVTDIVLNLKQVRLAMDTEEPQHLELHVNKSGEVKAGDIKCNQHVMVLNADQHLMTLTEDVELTFELEVRMG
KGYVPADMHEGLSDEIGLIALDASFSPVRKVAYTVEQARVGQMTNYDKLILEVWTDGSISPEDAIAYSAKIIKDQISVFI
NFDERISEQENEGRSSASDVNENLFKGIDELELSVRATNCLKSANISLVGELVQKSEGEMLKTKNFGRKSLDEIRRVLGE
MSLDFGMKVDGFEKKYQEWLKRKQQNEA
>Mature_348_residues
MLIKQGDRLINTRNWSELVKPDQISRASESADTMYGKFVCEPLERGYGTTIGNAMRRVLLASLQGAAFVAVKISGVQHEF
TTIPGVLEDVTDIVLNLKQVRLAMDTEEPQHLELHVNKSGEVKAGDIKCNQHVMVLNADQHLMTLTEDVELTFELEVRMG
KGYVPADMHEGLSDEIGLIALDASFSPVRKVAYTVEQARVGQMTNYDKLILEVWTDGSISPEDAIAYSAKIIKDQISVFI
NFDERISEQENEGRSSASDVNENLFKGIDELELSVRATNCLKSANISLVGELVQKSEGEMLKTKNFGRKSLDEIRRVLGE
MSLDFGMKVDGFEKKYQEWLKRKQQNEA

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family

Homologues:

Organism=Escherichia coli, GI1789690, Length=323, Percent_Identity=39.6284829721362, Blast_Score=227, Evalue=1e-60,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): RPOA_DESDG (Q30Z68)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388723.1
- ProteinModelPortal:   Q30Z68
- STRING:   Q30Z68
- GeneID:   3757242
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2231
- NMPDR:   fig|207559.3.peg.2193
- eggNOG:   COG0202
- HOGENOM:   HBG430844
- OMA:   FGTTLGN
- ProtClustDB:   PRK05182
- BioCyc:   DDES207559:DDE_2231-MONOMER
- HAMAP:   MF_00059
- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- Gene3D:   G3DSA:2.170.120.12
- ProDom:   PD001179
- SMART:   SM00662
- TIGRFAMs:   TIGR02027

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L; SSF47789 RNAP_alpha_C; SSF56553 RNAP_insert; SSF55257 RNAP_RBP11-like

EC number: =2.7.7.6

Molecular weight: Translated: 38993; Mature: 38993

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIKQGDRLINTRNWSELVKPDQISRASESADTMYGKFVCEPLERGYGTTIGNAMRRVLL
CCCCCCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ASLQGAAFVAVKISGVQHEFTTIPGVLEDVTDIVLNLKQVRLAMDTEEPQHLELHVNKSG
HHCCCCEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCC
EVKAGDIKCNQHVMVLNADQHLMTLTEDVELTFELEVRMGKGYVPADMHEGLSDEIGLIA
CEEECCEEECCEEEEEECCCCEEEEECCEEEEEEEEEEECCCCCCCHHHCCCCCCCCEEE
LDASFSPVRKVAYTVEQARVGQMTNYDKLILEVWTDGSISPEDAIAYSAKIIKDQISVFI
EECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEE
NFDERISEQENEGRSSASDVNENLFKGIDELELSVRATNCLKSANISLVGELVQKSEGEM
CCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
LKTKNFGRKSLDEIRRVLGEMSLDFGMKVDGFEKKYQEWLKRKQQNEA
EHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLIKQGDRLINTRNWSELVKPDQISRASESADTMYGKFVCEPLERGYGTTIGNAMRRVLL
CCCCCCCCEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
ASLQGAAFVAVKISGVQHEFTTIPGVLEDVTDIVLNLKQVRLAMDTEEPQHLELHVNKSG
HHCCCCEEEEEEEECCCCHHHHCCHHHHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCC
EVKAGDIKCNQHVMVLNADQHLMTLTEDVELTFELEVRMGKGYVPADMHEGLSDEIGLIA
CEEECCEEECCEEEEEECCCCEEEEECCEEEEEEEEEEECCCCCCCHHHCCCCCCCCEEE
LDASFSPVRKVAYTVEQARVGQMTNYDKLILEVWTDGSISPEDAIAYSAKIIKDQISVFI
EECCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHEEE
NFDERISEQENEGRSSASDVNENLFKGIDELELSVRATNCLKSANISLVGELVQKSEGEM
CCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCE
LKTKNFGRKSLDEIRRVLGEMSLDFGMKVDGFEKKYQEWLKRKQQNEA
EHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA