Click here to switch to the map view.

The map label for this gene is spsK [H]

Identifier: 78357226

GI number: 78357226

Start: 2223620

End: 2224504

Strand: Direct

Name: spsK [H]

Synonym: Dde_2183

Alternate gene names: 78357226

Gene position: 2223620-2224504 (Clockwise)

Preceding gene: 78357225

Following gene: 78357227

Centisome position: 59.61

GC content: 61.47

Gene sequence:

>885_bases
ATGACCGTGGCACCCAAGGCAGTGGTTCTGGGCGGGCGCACCGGCCTGCTGGGACAGGCGCTGGTGGCGGACCTGACCCG
TGCTGGCTGGCAGGTTGAAGCACACGGCAGAGAAGATGCCGATGTGCATGATTCTGCCGCGCTGGAAAAATACATCTGCT
CCCATGCTCCCGACGTGCTTTTCAATGCGGTGGCGTACACGCAGGTGGACAAGGCGGAAGAAGAGCAGGATGCCGCCATG
CGCCTGAACAAAAGTCTGCCCGCACTGCTGGGCAGACTGGCCTGCACCGGCAACATGCATCTTGTCCACTACAGCACCGA
CTTCGTCTTTAACGGACGCAAGGAAACGCCGTATACAGAAGATGACGAAACCGCCCCGCAGAGCATCTACGGCCTGTCCA
AGCTGGCCGGAGAACAGGTACTGCTGCAGATGAACCTGCCCCGCCTGACGATTATACGCAGCTCATGGCTTTTCGGTCCG
GGCAGGGGCAACTTTGTGCAGACCATACTGGGGCTGTGCGAGACGCGGCAGGAAATCACCGTGGTGCATGATCAGGTTGG
CTCGCCCACCTACACACCGGATCTTGCCGCCGGTTCACGCCTGCTGGTTGAAAAAGGGGGCACAGGTCTCTTTCACCTGA
GCAACGGCGGTCAGGCCACATGGTGCGAGCTGGCGGCTGAGGCGGTCTCGCTGGCGCAGAAGCCCTGTCAGGTCCGGCCT
ATCCCTTCGGCGGCTTATCCGCAGCGGGCCACCCGCCCTGCATATTCCGTTCTCGACTGCTCCCGGTTCACCGGCATTAC
GGGAATCAAACCGCGCCCCTGGATTCAGGCTCTGCGCGAGTATATCTTTCAGCACGTCGGCGTGGCCGGACAGGAAGCGG
GATAA

Upstream 100 bases:

>100_bases
CAGATACTCTGGCATGGTACGAAAAACACACAACATGGCTGGAAAACGTAAAAAGCGGGGCCTACCTTGAATTTATGTCC
CGCTGGTACGGAGACCGCTG

Downstream 100 bases:

>100_bases
CCGGCCCGCACTGCAGGCAGGGTGCCCGCGCTTTGCAAAGGAGTTACAGAATGGCTTTTCCCGCAGTGACGGCGGCTGTC
AGAAAAATATTCACCGATGC

Product: dTDP-4-dehydrorhamnose reductase

Products: dTDP-4-dehydro-6-deoxy-L-mannose; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTVAPKAVVLGGRTGLLGQALVADLTRAGWQVEAHGREDADVHDSAALEKYICSHAPDVLFNAVAYTQVDKAEEEQDAAM
RLNKSLPALLGRLACTGNMHLVHYSTDFVFNGRKETPYTEDDETAPQSIYGLSKLAGEQVLLQMNLPRLTIIRSSWLFGP
GRGNFVQTILGLCETRQEITVVHDQVGSPTYTPDLAAGSRLLVEKGGTGLFHLSNGGQATWCELAAEAVSLAQKPCQVRP
IPSAAYPQRATRPAYSVLDCSRFTGITGIKPRPWIQALREYIFQHVGVAGQEAG

Sequences:

>Translated_294_residues
MTVAPKAVVLGGRTGLLGQALVADLTRAGWQVEAHGREDADVHDSAALEKYICSHAPDVLFNAVAYTQVDKAEEEQDAAM
RLNKSLPALLGRLACTGNMHLVHYSTDFVFNGRKETPYTEDDETAPQSIYGLSKLAGEQVLLQMNLPRLTIIRSSWLFGP
GRGNFVQTILGLCETRQEITVVHDQVGSPTYTPDLAAGSRLLVEKGGTGLFHLSNGGQATWCELAAEAVSLAQKPCQVRP
IPSAAYPQRATRPAYSVLDCSRFTGITGIKPRPWIQALREYIFQHVGVAGQEAG
>Mature_293_residues
TVAPKAVVLGGRTGLLGQALVADLTRAGWQVEAHGREDADVHDSAALEKYICSHAPDVLFNAVAYTQVDKAEEEQDAAMR
LNKSLPALLGRLACTGNMHLVHYSTDFVFNGRKETPYTEDDETAPQSIYGLSKLAGEQVLLQMNLPRLTIIRSSWLFGPG
RGNFVQTILGLCETRQEITVVHDQVGSPTYTPDLAAGSRLLVEKGGTGLFHLSNGGQATWCELAAEAVSLAQKPCQVRPI
PSAAYPQRATRPAYSVLDCSRFTGITGIKPRPWIQALREYIFQHVGVAGQEAG

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Homo sapiens, GI33519455, Length=303, Percent_Identity=28.7128712871287, Blast_Score=96, Evalue=5e-20,
Organism=Homo sapiens, GI11034825, Length=303, Percent_Identity=28.7128712871287, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788352, Length=270, Percent_Identity=33.7037037037037, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: 1.1.1.133

Molecular weight: Translated: 31829; Mature: 31697

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVAPKAVVLGGRTGLLGQALVADLTRAGWQVEAHGREDADVHDSAALEKYICSHAPDVL
CCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCHHHH
FNAVAYTQVDKAEEEQDAAMRLNKSLPALLGRLACTGNMHLVHYSTDFVFNGRKETPYTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCC
DDETAPQSIYGLSKLAGEQVLLQMNLPRLTIIRSSWLFGPGRGNFVQTILGLCETRQEIT
CCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHE
VVHDQVGSPTYTPDLAAGSRLLVEKGGTGLFHLSNGGQATWCELAAEAVSLAQKPCQVRP
EHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
IPSAAYPQRATRPAYSVLDCSRFTGITGIKPRPWIQALREYIFQHVGVAGQEAG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
TVAPKAVVLGGRTGLLGQALVADLTRAGWQVEAHGREDADVHDSAALEKYICSHAPDVL
CCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHHHHHCCHHHH
FNAVAYTQVDKAEEEQDAAMRLNKSLPALLGRLACTGNMHLVHYSTDFVFNGRKETPYTE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCEEECCCCCCCCCC
DDETAPQSIYGLSKLAGEQVLLQMNLPRLTIIRSSWLFGPGRGNFVQTILGLCETRQEIT
CCCCCHHHHHHHHHHCCCEEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCHHHE
VVHDQVGSPTYTPDLAAGSRLLVEKGGTGLFHLSNGGQATWCELAAEAVSLAQKPCQVRP
EHHHCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCC
IPSAAYPQRATRPAYSVLDCSRFTGITGIKPRPWIQALREYIFQHVGVAGQEAG
CCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: dTDP-6-deoxy-L-mannose; NADP+

Specific reaction: dTDP-6-deoxy-L-mannose + NADP+ = dTDP-4-dehydro-6-deoxy-L-mannose + NADPH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934828; 9384377; 10568751 [H]