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The map label for this gene is rfbB [H]

Identifier: 78357225

GI number: 78357225

Start: 2222598

End: 2223620

Strand: Direct

Name: rfbB [H]

Synonym: Dde_2182

Alternate gene names: 78357225

Gene position: 2222598-2223620 (Clockwise)

Preceding gene: 78357220

Following gene: 78357226

Centisome position: 59.58

GC content: 56.79

Gene sequence:

>1023_bases
ATGCGACTACTCGTCACTGGCGGCTGCGGCTTCATCGGCACCAACTTTATCCGGTACCAGCTTAACGTCCATCCGCAGAC
CACCATTGTCAACTTAGATAAACTGACCTACGCGGGAAACCCCCAGAATCTTACCGACATTCAGCAAGCCCACGGCGGCA
CCCGCTACTTTTTTGAAAAAGCGGATATCGCTGATGCGGCGGCTGTTCAGGCTGTAATAGAAAAGTACAGCCCCGACGCC
GTACTCAATTTTGCCGCGGAATCACATGTGGACCGGTCCATCTCGGACCCGGCACCTTTTGTAACCACCAATGTGCTGGG
TACTCAGGTGCTTATGCAGGCGGCCCGTACAGCAGGAATAAAGCGTTTTGTCCACATATCCACGGACGAGGTGTACGGTT
CGCTGCTGCCCCACGAGGCACCCTTCACGGAAAGCAACCCTCTGCTGCCCAACAGCCCCTATTCGGCCTCAAAGGCCGGG
GCGGACCTCATGGTCCGGGCTTTTGTGGAAACATACGGCTTTCCCGCCATCATCACCCGCTGTTCCAACAACTACGGACC
GTACCAGTTTCCGGAAAAGCTCATTCCGCTCATGCTGGGCAAAGCATGGGAAAACGCCCCCCTGCCCGTCTACGGCGACG
GCACCAACGTCCGTGACTGGATTCACGTGGAAGACCACTGCCGGGGCATTGACCTTGCCCTGCGCAAAGGCCGTGACGGC
GCCGTATACAATTTCGGCGGCAATGCCGAACGCACCAACCTTGATGTGGTCAGAGCCATACTGCGCCTGACCGGAAAGCC
CGAGTCGCTCATATCCTTTGTGAAAGACAGGCCCGGACACGACAGGCGCTATGCCATGGACTTCACTCTGGCCGGCGACG
AACTGGGGTACGCCCCCGTGCATGATTTCGAAACAGGACTTGCAGATACTCTGGCATGGTACGAAAAACACACAACATGG
CTGGAAAACGTAAAAAGCGGGGCCTACCTTGAATTTATGTCCCGCTGGTACGGAGACCGCTGA

Upstream 100 bases:

>100_bases
ATGCCATGCAGTCCGCCGCGGCATTATGCGCGTTTTTGGGTTTGACCGGGTACCGGACGTACAGGTATGTAGAGGGGTCA
AACCAAGTGAGGAACCCTGC

Downstream 100 bases:

>100_bases
TGACCGTGGCACCCAAGGCAGTGGTTCTGGGCGGGCGCACCGGCCTGCTGGGACAGGCGCTGGTGGCGGACCTGACCCGT
GCTGGCTGGCAGGTTGAAGC

Product: dTDP-glucose 4,6-dehydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 340

Protein sequence:

>340_residues
MRLLVTGGCGFIGTNFIRYQLNVHPQTTIVNLDKLTYAGNPQNLTDIQQAHGGTRYFFEKADIADAAAVQAVIEKYSPDA
VLNFAAESHVDRSISDPAPFVTTNVLGTQVLMQAARTAGIKRFVHISTDEVYGSLLPHEAPFTESNPLLPNSPYSASKAG
ADLMVRAFVETYGFPAIITRCSNNYGPYQFPEKLIPLMLGKAWENAPLPVYGDGTNVRDWIHVEDHCRGIDLALRKGRDG
AVYNFGGNAERTNLDVVRAILRLTGKPESLISFVKDRPGHDRRYAMDFTLAGDELGYAPVHDFETGLADTLAWYEKHTTW
LENVKSGAYLEFMSRWYGDR

Sequences:

>Translated_340_residues
MRLLVTGGCGFIGTNFIRYQLNVHPQTTIVNLDKLTYAGNPQNLTDIQQAHGGTRYFFEKADIADAAAVQAVIEKYSPDA
VLNFAAESHVDRSISDPAPFVTTNVLGTQVLMQAARTAGIKRFVHISTDEVYGSLLPHEAPFTESNPLLPNSPYSASKAG
ADLMVRAFVETYGFPAIITRCSNNYGPYQFPEKLIPLMLGKAWENAPLPVYGDGTNVRDWIHVEDHCRGIDLALRKGRDG
AVYNFGGNAERTNLDVVRAILRLTGKPESLISFVKDRPGHDRRYAMDFTLAGDELGYAPVHDFETGLADTLAWYEKHTTW
LENVKSGAYLEFMSRWYGDR
>Mature_340_residues
MRLLVTGGCGFIGTNFIRYQLNVHPQTTIVNLDKLTYAGNPQNLTDIQQAHGGTRYFFEKADIADAAAVQAVIEKYSPDA
VLNFAAESHVDRSISDPAPFVTTNVLGTQVLMQAARTAGIKRFVHISTDEVYGSLLPHEAPFTESNPLLPNSPYSASKAG
ADLMVRAFVETYGFPAIITRCSNNYGPYQFPEKLIPLMLGKAWENAPLPVYGDGTNVRDWIHVEDHCRGIDLALRKGRDG
AVYNFGGNAERTNLDVVRAILRLTGKPESLISFVKDRPGHDRRYAMDFTLAGDELGYAPVHDFETGLADTLAWYEKHTTW
LENVKSGAYLEFMSRWYGDR

Specific function: Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction [H]

COG id: COG1088

COG function: function code M; dTDP-D-glucose 4,6-dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=332, Percent_Identity=36.4457831325301, Blast_Score=223, Evalue=2e-58,
Organism=Homo sapiens, GI56237023, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI56118217, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI189083684, Length=341, Percent_Identity=28.4457478005865, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI42516563, Length=330, Percent_Identity=24.8484848484848, Blast_Score=96, Evalue=4e-20,
Organism=Homo sapiens, GI4504031, Length=327, Percent_Identity=25.0764525993884, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1788353, Length=365, Percent_Identity=49.041095890411, Blast_Score=341, Evalue=3e-95,
Organism=Escherichia coli, GI48994969, Length=348, Percent_Identity=47.9885057471264, Blast_Score=325, Evalue=4e-90,
Organism=Escherichia coli, GI1786974, Length=344, Percent_Identity=29.6511627906977, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1788366, Length=181, Percent_Identity=28.1767955801105, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17568069, Length=324, Percent_Identity=34.8765432098765, Blast_Score=211, Evalue=4e-55,
Organism=Caenorhabditis elegans, GI115532424, Length=320, Percent_Identity=34.375, Blast_Score=183, Evalue=1e-46,
Organism=Caenorhabditis elegans, GI71982035, Length=347, Percent_Identity=27.0893371757925, Blast_Score=97, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71982038, Length=349, Percent_Identity=26.9340974212034, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17539532, Length=319, Percent_Identity=22.5705329153605, Blast_Score=72, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319493, Length=292, Percent_Identity=27.0547945205479, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI19923002, Length=344, Percent_Identity=29.6511627906977, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI21356223, Length=318, Percent_Identity=25.4716981132075, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24158427, Length=195, Percent_Identity=27.1794871794872, Blast_Score=75, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005888
- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.46 [H]

Molecular weight: Translated: 37770; Mature: 37770

Theoretical pI: Translated: 6.18; Mature: 6.18

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLLVTGGCGFIGTNFIRYQLNVHPQTTIVNLDKLTYAGNPQNLTDIQQAHGGTRYFFEK
CEEEEECCCCHHCCCEEEEEEECCCCEEEEEEHHEEECCCCCCHHHHHHHCCCCEEEEEC
ADIADAAAVQAVIEKYSPDAVLNFAAESHVDRSISDPAPFVTTNVLGTQVLMQAARTAGI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
KRFVHISTDEVYGSLLPHEAPFTESNPLLPNSPYSASKAGADLMVRAFVETYGFPAIITR
HEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
CSNNYGPYQFPEKLIPLMLGKAWENAPLPVYGDGTNVRDWIHVEDHCRGIDLALRKGRDG
HCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEHHHCCCCHHHHCCCCCC
AVYNFGGNAERTNLDVVRAILRLTGKPESLISFVKDRPGHDRRYAMDFTLAGDELGYAPV
CEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCC
HDFETGLADTLAWYEKHTTWLENVKSGAYLEFMSRWYGDR
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MRLLVTGGCGFIGTNFIRYQLNVHPQTTIVNLDKLTYAGNPQNLTDIQQAHGGTRYFFEK
CEEEEECCCCHHCCCEEEEEEECCCCEEEEEEHHEEECCCCCCHHHHHHHCCCCEEEEEC
ADIADAAAVQAVIEKYSPDAVLNFAAESHVDRSISDPAPFVTTNVLGTQVLMQAARTAGI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHH
KRFVHISTDEVYGSLLPHEAPFTESNPLLPNSPYSASKAGADLMVRAFVETYGFPAIITR
HEEEEECHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHH
CSNNYGPYQFPEKLIPLMLGKAWENAPLPVYGDGTNVRDWIHVEDHCRGIDLALRKGRDG
HCCCCCCCCCHHHHHHHHHHCCCCCCCCEEECCCCCCCEEEEEHHHCCCCHHHHCCCCCC
AVYNFGGNAERTNLDVVRAILRLTGKPESLISFVKDRPGHDRRYAMDFTLAGDELGYAPV
CEEECCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCC
HDFETGLADTLAWYEKHTTWLENVKSGAYLEFMSRWYGDR
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609; 11796113 [H]