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The map label for this gene is lspA

Identifier: 78357186

GI number: 78357186

Start: 2191794

End: 2192264

Strand: Direct

Name: lspA

Synonym: Dde_2143

Alternate gene names: 78357186

Gene position: 2191794-2192264 (Clockwise)

Preceding gene: 78357185

Following gene: 78357187

Centisome position: 58.76

GC content: 53.08

Gene sequence:

>471_bases
ATGCGTCCACGTTTTTTCGTCGTGTACCTCATCGCCGCACTTGTCATTGCCGTTGACCAGTGGACAAAGCAATGGGCAGC
CTCCACCATCCCTCCGCTTATCGGCTATACAGTTATTCCCGGTTTTTTCGACCTCGTAAACGTGCGTAACCGCGGCGCTG
CTTTCGGCTTTTTAAACAGAAGCGACATAGAATGGCAGTTCTGGCTCTTTTTCGCCGCTGCCGTCACAGCCGTCCTCGCC
ATCATTGCCATGACACGCTCCGCCAAAAGCAATCCCTACCTGTTTACAGGGTTCGGGCTTATCATGGGGGGAGCCATCGG
CAACCTCATTGACAGAATCCGCTTCCGCGCCGTTATCGACTTTCTTGATTTCCACATAGGCGGGTATCACTGGCCCGCAT
TCAACGTGGCGGATATGGGCATCTGCGTCGGAGCATTTTTTGTCTGCCTGGCAGTGTACAAACACAAATAA

Upstream 100 bases:

>100_bases
TCGTTGTGCCCCCGGTGCACCGGCGTACTCTGCGCCTGATACTGATCATCTGCGCGGGCCCGCACTGCGGGTCCGCGCTA
TCTCCATACAGAGGAAAGCC

Downstream 100 bases:

>100_bases
CCTGCGACCGGAGAAATTCAGTCATGCTCCCACTGGTTGACCTTGTACTGCAAACTGATAATCTTCCGCTGATGATGCTG
CTGCTTGCCGCACCTATCAT

Product: signal peptidase II

Products: NA

Alternate protein names: Prolipoprotein signal peptidase; Signal peptidase II; SPase II

Number of amino acids: Translated: 156; Mature: 156

Protein sequence:

>156_residues
MRPRFFVVYLIAALVIAVDQWTKQWAASTIPPLIGYTVIPGFFDLVNVRNRGAAFGFLNRSDIEWQFWLFFAAAVTAVLA
IIAMTRSAKSNPYLFTGFGLIMGGAIGNLIDRIRFRAVIDFLDFHIGGYHWPAFNVADMGICVGAFFVCLAVYKHK

Sequences:

>Translated_156_residues
MRPRFFVVYLIAALVIAVDQWTKQWAASTIPPLIGYTVIPGFFDLVNVRNRGAAFGFLNRSDIEWQFWLFFAAAVTAVLA
IIAMTRSAKSNPYLFTGFGLIMGGAIGNLIDRIRFRAVIDFLDFHIGGYHWPAFNVADMGICVGAFFVCLAVYKHK
>Mature_156_residues
MRPRFFVVYLIAALVIAVDQWTKQWAASTIPPLIGYTVIPGFFDLVNVRNRGAAFGFLNRSDIEWQFWLFFAAAVTAVLA
IIAMTRSAKSNPYLFTGFGLIMGGAIGNLIDRIRFRAVIDFLDFHIGGYHWPAFNVADMGICVGAFFVCLAVYKHK

Specific function: This protein specifically catalyzes the removal of signal peptides from prolipoproteins

COG id: COG0597

COG function: function code MU; Lipoprotein signal peptidase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A8 family

Homologues:

Organism=Escherichia coli, GI1786210, Length=110, Percent_Identity=41.8181818181818, Blast_Score=79, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LSPA_DESDG (Q30ZF6)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388635.1
- STRING:   Q30ZF6
- MEROPS:   A08.001
- GeneID:   3757152
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2143
- NMPDR:   fig|207559.3.peg.1930
- eggNOG:   COG0597
- HOGENOM:   HBG724422
- OMA:   VIDYADL
- BioCyc:   DDES207559:DDE_2143-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_00161
- InterPro:   IPR001872
- PRINTS:   PR00781
- TIGRFAMs:   TIGR00077

Pfam domain/function: PF01252 Peptidase_A8

EC number: =3.4.23.36

Molecular weight: Translated: 17476; Mature: 17476

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: PS00855 SPASE_II

Important sites: ACT_SITE 111-111 ACT_SITE 138-138

Signals:

None

Transmembrane regions:

HASH(0x1299da08)-; HASH(0x12768714)-; HASH(0x408709f8)-; HASH(0x4086fc30)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPRFFVVYLIAALVIAVDQWTKQWAASTIPPLIGYTVIPGFFDLVNVRNRGAAFGFLNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCC
SDIEWQFWLFFAAAVTAVLAIIAMTRSAKSNPYLFTGFGLIMGGAIGNLIDRIRFRAVID
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
FLDFHIGGYHWPAFNVADMGICVGAFFVCLAVYKHK
HHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MRPRFFVVYLIAALVIAVDQWTKQWAASTIPPLIGYTVIPGFFDLVNVRNRGAAFGFLNR
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCC
SDIEWQFWLFFAAAVTAVLAIIAMTRSAKSNPYLFTGFGLIMGGAIGNLIDRIRFRAVID
CCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHH
FLDFHIGGYHWPAFNVADMGICVGAFFVCLAVYKHK
HHHHHCCCEECCCCCHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA