Click here to switch to the map view.

The map label for this gene is tesA [H]

Identifier: 78357184

GI number: 78357184

Start: 2188240

End: 2188818

Strand: Direct

Name: tesA [H]

Synonym: Dde_2141

Alternate gene names: 78357184

Gene position: 2188240-2188818 (Clockwise)

Preceding gene: 78357183

Following gene: 78357185

Centisome position: 58.66

GC content: 57.17

Gene sequence:

>579_bases
GTGCTTCCGCCTTTTACCATCCTTGCTCTGGGAGACAGCCTGACTGAAGGTTACGGCCTTCCGGCGGGATGTGCTGTTCC
CGATGTTCTCCGGCAGCTGCTGGAAAAATCAGGTACCCGCGTCCGCATTGTGAATCTGGGACTTTCCGGCGATACAACAG
CAGGCGGCCTCAGAAGACTGCGGGCATGGCTTTCGCGGCACCCGGCTCCCCATGCCGCCATGGTGGAGCTGGGTGTCAAC
GATACATTCATGGGACTGGAATACGAAGAAATTGAAGACAACCTTGATGCCATCCTGCAGATATTGACAGCTCAGGATGT
TCCGGTCCTTCTTACCTCCATGCCGTTACTTTTTCCCGCACATGAAGAAGACCGAAAGGCGTTTGACGCCATCTATTCGA
ATCTCGCCGCGCGTCACCGTGTAATGCTTCACCCCTTTTTTCCCGAGGGCGTTGCGGCAAATCCTGCTCTGAGCCTGCCC
GACGGCATTCACCCTGCCCCCGCAGGCGCACATGCCATCGCCGAACATCTTCTGCCTGCCGTACAAAAACTGCTGTGCAA
CGCCGCCGGCAAAGCCTGA

Upstream 100 bases:

>100_bases
TTGCCCTGCGCAAGCTGGACCCCGCAGAAGCACAGGAGAGCCTGCGTCTGCTCCGGCAGATGGCCAACATCGAAGAAGGA
ACTCCCGGAGGCTTTTAGCC

Downstream 100 bases:

>100_bases
CAACTCCGCACCGGTGTATGCCTGCACCCTTGACTTTACATCACGCTTGCACCATGTTTCGAAGTTTACAACGTTCCGTA
TCCGATGCTTCCTGAGGAGA

Product: GDSL family lipase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 192; Mature: 192

Protein sequence:

>192_residues
MLPPFTILALGDSLTEGYGLPAGCAVPDVLRQLLEKSGTRVRIVNLGLSGDTTAGGLRRLRAWLSRHPAPHAAMVELGVN
DTFMGLEYEEIEDNLDAILQILTAQDVPVLLTSMPLLFPAHEEDRKAFDAIYSNLAARHRVMLHPFFPEGVAANPALSLP
DGIHPAPAGAHAIAEHLLPAVQKLLCNAAGKA

Sequences:

>Translated_192_residues
MLPPFTILALGDSLTEGYGLPAGCAVPDVLRQLLEKSGTRVRIVNLGLSGDTTAGGLRRLRAWLSRHPAPHAAMVELGVN
DTFMGLEYEEIEDNLDAILQILTAQDVPVLLTSMPLLFPAHEEDRKAFDAIYSNLAARHRVMLHPFFPEGVAANPALSLP
DGIHPAPAGAHAIAEHLLPAVQKLLCNAAGKA
>Mature_192_residues
MLPPFTILALGDSLTEGYGLPAGCAVPDVLRQLLEKSGTRVRIVNLGLSGDTTAGGLRRLRAWLSRHPAPHAAMVELGVN
DTFMGLEYEEIEDNLDAILQILTAQDVPVLLTSMPLLFPAHEEDRKAFDAIYSNLAARHRVMLHPFFPEGVAANPALSLP
DGIHPAPAGAHAIAEHLLPAVQKLLCNAAGKA

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=182, Percent_Identity=40.1098901098901, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 20517; Mature: 20517

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPPFTILALGDSLTEGYGLPAGCAVPDVLRQLLEKSGTRVRIVNLGLSGDTTAGGLRRL
CCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
RAWLSRHPAPHAAMVELGVNDTFMGLEYEEIEDNLDAILQILTAQDVPVLLTSMPLLFPA
HHHHHHCCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHCCC
HEEDRKAFDAIYSNLAARHRVMLHPFFPEGVAANPALSLPDGIHPAPAGAHAIAEHLLPA
CHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VQKLLCNAAGKA
HHHHHHHHCCCC
>Mature Secondary Structure
MLPPFTILALGDSLTEGYGLPAGCAVPDVLRQLLEKSGTRVRIVNLGLSGDTTAGGLRRL
CCCCEEEEEECCHHHHCCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHH
RAWLSRHPAPHAAMVELGVNDTFMGLEYEEIEDNLDAILQILTAQDVPVLLTSMPLLFPA
HHHHHHCCCCCCEEEEECCCCEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHCCC
HEEDRKAFDAIYSNLAARHRVMLHPFFPEGVAANPALSLPDGIHPAPAGAHAIAEHLLPA
CHHHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
VQKLLCNAAGKA
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]