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The map label for this gene is sigB [H]

Identifier: 78357159

GI number: 78357159

Start: 2165550

End: 2166632

Strand: Direct

Name: sigB [H]

Synonym: Dde_2116

Alternate gene names: 78357159

Gene position: 2165550-2166632 (Clockwise)

Preceding gene: 78357152

Following gene: 78357160

Centisome position: 58.05

GC content: 56.05

Gene sequence:

>1083_bases
ATGACTCCCGCAAAAGATACCGTAAAAAAGCATACCGCAGCCGCAGCCACGGACAGCAACGCCGCAGAAGATTCCGCGGC
GGAAGTCCTGACCGGAGACGACGAAACGACCTATGATATCGAAATTGATCAGGAAGAAGACGATGAAGATATCGATATCG
ACGAGGGCGATGTCATCGACGTCAGCGATACGGACCTGCCTGCCCCCCTGCCCCCCGGCAACAAGCCCACGCGCGATTCG
CTGCATCTGTACCTGCGCGAAATAAGCCGTTTTCCCATGCTCAAGCCCGACGAAGAGTTTGAGCTTGCACGGCGGGTACA
GCTGCACAACGACAGTGATGCGGCCTTCCGGCTTGTATCATCGCATCTGCGGCTTGTGGTCAAGATTGCCATGGACTTTC
AGCGCCGCTGGATGCAGAACGTGCTCGACCTGATACAGGAAGGCAACGTGGGGCTGATGCGTGCCGTCAACAAGTTTGAC
CCGGACAAGGGCATAAAATTTTCATACTACGCCGCTTTCTGGATAAAGGCGTATATCCTCAAGTTCATCATGGACAACTG
GCGGCTGGTCAAAATAGGCACCACGCAGGCCCAGCGGAAGCTTTTCTACAACCTTAACAAAGAACGCCAGAAGCTTATAG
CGCAGGGGTACGACCCCGATGCGGCCATGCTTTCCGAGCGGCTGGACGTCACCACCGAGCAGGTGGTGGAAATGGAGCAG
CGGCTTGACGCTTCGGACATGTCGCTTGATGTGCAGGTCGGCGACGAAAACGGCTCGGCTTCGCGCATGGACTTTCTGCC
GGCGCTCGGTCCCGGCATTGAAGACACCCTCGCCAACAACGAAATCGCGGCAATGGTGCAGGAACGCATCCGGGATATCG
TCCCCCTGCTCAATGACAAGGAGCTGGACATACTCAACTCGCGCCTGCTTTCGGAAGACCCGGTCACCCTGCGCGAAATA
GGCGAAAAGTATGATATAACCCGCGAACGGGTCCGCCAGATAGAAGCCCGTTTGCTCCAGAAGATCCGTGACCACCTGTT
CAAGGAAATCAAGGATTTCTCTTCGGACTGGATCACCAGATAG

Upstream 100 bases:

>100_bases
ATACCATTCAATGCCCAAGCCTGTTCTTAAGAGCAGGACTTCCCATCAAAGATCATATCCGGCTGATGCCGGTTAAAGCT
CCGGATAACACTCTATATCT

Downstream 100 bases:

>100_bases
CAGAATACACTTACAAGAGAGACTGTCATGTTTTCCTTCAGGCACATCACGCTGGCAACAGCGTTGTGCGCGTCACTCAC
TGCCGGATGCGCCACAGGAC

Product: sigma 70 family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTPAKDTVKKHTAAAATDSNAAEDSAAEVLTGDDETTYDIEIDQEEDDEDIDIDEGDVIDVSDTDLPAPLPPGNKPTRDS
LHLYLREISRFPMLKPDEEFELARRVQLHNDSDAAFRLVSSHLRLVVKIAMDFQRRWMQNVLDLIQEGNVGLMRAVNKFD
PDKGIKFSYYAAFWIKAYILKFIMDNWRLVKIGTTQAQRKLFYNLNKERQKLIAQGYDPDAAMLSERLDVTTEQVVEMEQ
RLDASDMSLDVQVGDENGSASRMDFLPALGPGIEDTLANNEIAAMVQERIRDIVPLLNDKELDILNSRLLSEDPVTLREI
GEKYDITRERVRQIEARLLQKIRDHLFKEIKDFSSDWITR

Sequences:

>Translated_360_residues
MTPAKDTVKKHTAAAATDSNAAEDSAAEVLTGDDETTYDIEIDQEEDDEDIDIDEGDVIDVSDTDLPAPLPPGNKPTRDS
LHLYLREISRFPMLKPDEEFELARRVQLHNDSDAAFRLVSSHLRLVVKIAMDFQRRWMQNVLDLIQEGNVGLMRAVNKFD
PDKGIKFSYYAAFWIKAYILKFIMDNWRLVKIGTTQAQRKLFYNLNKERQKLIAQGYDPDAAMLSERLDVTTEQVVEMEQ
RLDASDMSLDVQVGDENGSASRMDFLPALGPGIEDTLANNEIAAMVQERIRDIVPLLNDKELDILNSRLLSEDPVTLREI
GEKYDITRERVRQIEARLLQKIRDHLFKEIKDFSSDWITR
>Mature_359_residues
TPAKDTVKKHTAAAATDSNAAEDSAAEVLTGDDETTYDIEIDQEEDDEDIDIDEGDVIDVSDTDLPAPLPPGNKPTRDSL
HLYLREISRFPMLKPDEEFELARRVQLHNDSDAAFRLVSSHLRLVVKIAMDFQRRWMQNVLDLIQEGNVGLMRAVNKFDP
DKGIKFSYYAAFWIKAYILKFIMDNWRLVKIGTTQAQRKLFYNLNKERQKLIAQGYDPDAAMLSERLDVTTEQVVEMEQR
LDASDMSLDVQVGDENGSASRMDFLPALGPGIEDTLANNEIAAMVQERIRDIVPLLNDKELDILNSRLLSEDPVTLREIG
EKYDITRERVRQIEARLLQKIRDHLFKEIKDFSSDWITR

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=279, Percent_Identity=37.9928315412186, Blast_Score=178, Evalue=4e-46,
Organism=Escherichia coli, GI1789098, Length=319, Percent_Identity=33.8557993730408, Blast_Score=134, Evalue=1e-32,
Organism=Escherichia coli, GI1789448, Length=234, Percent_Identity=35.042735042735, Blast_Score=120, Evalue=9e-29,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 41232; Mature: 41101

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAKDTVKKHTAAAATDSNAAEDSAAEVLTGDDETTYDIEIDQEEDDEDIDIDEGDVID
CCCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEE
VSDTDLPAPLPPGNKPTRDSLHLYLREISRFPMLKPDEEFELARRVQLHNDSDAAFRLVS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SHLRLVVKIAMDFQRRWMQNVLDLIQEGNVGLMRAVNKFDPDKGIKFSYYAAFWIKAYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHH
KFIMDNWRLVKIGTTQAQRKLFYNLNKERQKLIAQGYDPDAAMLSERLDVTTEQVVEMEQ
HHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
RLDASDMSLDVQVGDENGSASRMDFLPALGPGIEDTLANNEIAAMVQERIRDIVPLLNDK
HCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCC
ELDILNSRLLSEDPVTLREIGEKYDITRERVRQIEARLLQKIRDHLFKEIKDFSSDWITR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TPAKDTVKKHTAAAATDSNAAEDSAAEVLTGDDETTYDIEIDQEEDDEDIDIDEGDVID
CCCHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEE
VSDTDLPAPLPPGNKPTRDSLHLYLREISRFPMLKPDEEFELARRVQLHNDSDAAFRLVS
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCHHHHHHHH
SHLRLVVKIAMDFQRRWMQNVLDLIQEGNVGLMRAVNKFDPDKGIKFSYYAAFWIKAYIL
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHH
KFIMDNWRLVKIGTTQAQRKLFYNLNKERQKLIAQGYDPDAAMLSERLDVTTEQVVEMEQ
HHHHCCCEEEEECCHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHH
RLDASDMSLDVQVGDENGSASRMDFLPALGPGIEDTLANNEIAAMVQERIRDIVPLLNDK
HCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHCCCC
ELDILNSRLLSEDPVTLREIGEKYDITRERVRQIEARLLQKIRDHLFKEIKDFSSDWITR
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1475207 [H]