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The map label for this gene is otsA [H]

Identifier: 78357103

GI number: 78357103

Start: 2103139

End: 2104593

Strand: Reverse

Name: otsA [H]

Synonym: Dde_2060

Alternate gene names: 78357103

Gene position: 2104593-2103139 (Counterclockwise)

Preceding gene: 78357104

Following gene: 78357102

Centisome position: 56.42

GC content: 56.56

Gene sequence:

>1455_bases
ATGACGCCAGCAAAGGGCCTGATCGTCGTATCCAACCGGCTTCCCGTCGTGTTGTCCAGAAATGAAGACGGGTGGGGAAT
AACGACCGGTGCCGGAGGGCTGGTTTCTGCCATGGCTCCGGTACTGAGAGACCGGGGAGGCACCTGGGTGGGATGGACCG
GCGCCAGCGGTGAACATGAGGCCGAATCACTTCTGGGCGAGTTTTCCCGTGAGGCCGGCTATTCCCTCAAGCCGGTCAGG
CTGGAGAAAGAAGATGTGGCCGATTTTTACTATGGCTTTGCCAACGAGGTTATCTGGCCGCTGTTTCATGATTTCCAGTC
GCTGTGCAATTTTTTTCCGAAATACTGGAAATCGTACCTGCGGGTGAACGAGTTGTATGCCCGTGCTGTCATGGATTCCG
GCGATCCGGAAGCGTACGTCTGGGTGCATGACTATCACCTGATGCATGTGGGCGAAATGCTCAAGTCCATGGGCAGCAGG
CGCAGGGCAGGTTTTTTTCTGCACATTCCTTTTCCCCCGATGGATAATTATCTGAAGCTGCCGTGGCGGCGCAGACTGCT
GTCCGCGTTGCTGGAGTATGATCTTGTGGGGTTTCAGACCTATCGTGACCGCAGAAATTTCATGCAGTCGGTACAGGTGC
TGCTTCCTCATGTGAAACCGCAGGGGAGGGGGCCTGTAATCGAACTGCTGCTGGACGGGCGCAAGGTAAGGCTGGGGGCT
TTTCCCATCAGCATCGATTACCGGTCTTTTGTCCGTACGGCACGTTCTCCTGCGGTGTCCGGCAGGGTGAACGAGCTGAA
AAGCGCCATGGACGGGCGCAGGATGCTTCTGGCCGTGGACCGGCTTGATTATTCTAAAGGCATAACATTCCGGCTGGAGG
CCTTTCGGGAAGCGCTGGAAAAATACCCCGGACTGCGGGGCAATGTCACCCTGATGCAGATAATGGTTCCCAGCCGGCAG
GAGGTGCCCAGATACAACGCCCTGAAGCAGGAAATTGAAAGGCTGGTCGGGGAAATCAACGGGCTGTTCAGCAGACCGGG
CTGGAATCCTGTGCAGTACCAGTACAGAAGCCTTGGCAGGGAAGAGCTTGTGGCGTATTACAGGGCCGCTTCGATGGCCA
TAGTCACCCCTCTGCGTGACGGCATGAATCTGGTGGCCAAAGAATACTGCGCCTGCAATGTGAACCGGCGGGGCGTGCTG
TTGCTCAGCGAATTTGCCGGTGCCGCAGCGCAGCTGCAGAACGGAGCCGTGCTGGTGAACCCTTTTGACAGGGAAGGCAT
GGCGCAGAGCATCAAAGAGGCTCTGGATATGCCTGCCGAGGAAGCACGCCGCAGGATGGACAGAATGCGGGACGTGATTC
GAAAAAATGATATTTTCCGCTGGGTGGATATGTTTCTGCTGGCTGCGTTTTCGCGCCATCTTGATGACTTTCCGCCGACC
TATGCCCGTAAATAA

Upstream 100 bases:

>100_bases
GATTGCCTTTGTTTTTTTCCGCGTAATTCTGCTCCGGGTTGTCCGGCAGTGCCGTTGCCGTTATAGTTTCCTCCACTGCA
ATTATCAGAAGGAGGCTTCC

Downstream 100 bases:

>100_bases
TGATTGATAAGGAATACCAGCGTATTATGACCGAACGAACCGCATCTGCGCCACGGGCGAAGAAAAGCCTGGGCCAGAAT
TTTCTGCAGGACAAGAATAT

Product: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Products: H+; alpha,alpha'-Trehalose 6-phosphate; UDP [C]

Alternate protein names: TPS; Trehalose-6-phosphate synthase [H]

Number of amino acids: Translated: 484; Mature: 483

Protein sequence:

>484_residues
MTPAKGLIVVSNRLPVVLSRNEDGWGITTGAGGLVSAMAPVLRDRGGTWVGWTGASGEHEAESLLGEFSREAGYSLKPVR
LEKEDVADFYYGFANEVIWPLFHDFQSLCNFFPKYWKSYLRVNELYARAVMDSGDPEAYVWVHDYHLMHVGEMLKSMGSR
RRAGFFLHIPFPPMDNYLKLPWRRRLLSALLEYDLVGFQTYRDRRNFMQSVQVLLPHVKPQGRGPVIELLLDGRKVRLGA
FPISIDYRSFVRTARSPAVSGRVNELKSAMDGRRMLLAVDRLDYSKGITFRLEAFREALEKYPGLRGNVTLMQIMVPSRQ
EVPRYNALKQEIERLVGEINGLFSRPGWNPVQYQYRSLGREELVAYYRAASMAIVTPLRDGMNLVAKEYCACNVNRRGVL
LLSEFAGAAAQLQNGAVLVNPFDREGMAQSIKEALDMPAEEARRRMDRMRDVIRKNDIFRWVDMFLLAAFSRHLDDFPPT
YARK

Sequences:

>Translated_484_residues
MTPAKGLIVVSNRLPVVLSRNEDGWGITTGAGGLVSAMAPVLRDRGGTWVGWTGASGEHEAESLLGEFSREAGYSLKPVR
LEKEDVADFYYGFANEVIWPLFHDFQSLCNFFPKYWKSYLRVNELYARAVMDSGDPEAYVWVHDYHLMHVGEMLKSMGSR
RRAGFFLHIPFPPMDNYLKLPWRRRLLSALLEYDLVGFQTYRDRRNFMQSVQVLLPHVKPQGRGPVIELLLDGRKVRLGA
FPISIDYRSFVRTARSPAVSGRVNELKSAMDGRRMLLAVDRLDYSKGITFRLEAFREALEKYPGLRGNVTLMQIMVPSRQ
EVPRYNALKQEIERLVGEINGLFSRPGWNPVQYQYRSLGREELVAYYRAASMAIVTPLRDGMNLVAKEYCACNVNRRGVL
LLSEFAGAAAQLQNGAVLVNPFDREGMAQSIKEALDMPAEEARRRMDRMRDVIRKNDIFRWVDMFLLAAFSRHLDDFPPT
YARK
>Mature_483_residues
TPAKGLIVVSNRLPVVLSRNEDGWGITTGAGGLVSAMAPVLRDRGGTWVGWTGASGEHEAESLLGEFSREAGYSLKPVRL
EKEDVADFYYGFANEVIWPLFHDFQSLCNFFPKYWKSYLRVNELYARAVMDSGDPEAYVWVHDYHLMHVGEMLKSMGSRR
RAGFFLHIPFPPMDNYLKLPWRRRLLSALLEYDLVGFQTYRDRRNFMQSVQVLLPHVKPQGRGPVIELLLDGRKVRLGAF
PISIDYRSFVRTARSPAVSGRVNELKSAMDGRRMLLAVDRLDYSKGITFRLEAFREALEKYPGLRGNVTLMQIMVPSRQE
VPRYNALKQEIERLVGEINGLFSRPGWNPVQYQYRSLGREELVAYYRAASMAIVTPLRDGMNLVAKEYCACNVNRRGVLL
LSEFAGAAAQLQNGAVLVNPFDREGMAQSIKEALDMPAEEARRRMDRMRDVIRKNDIFRWVDMFLLAAFSRHLDDFPPTY
ARK

Specific function: Catalyzes the transfer of glucose from a nucleoside diphosphate-glucose to glucose-6-phosphate to form trehalose-6- phosphate and nucleoside diphosphate [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=461, Percent_Identity=36.2255965292842, Blast_Score=280, Evalue=2e-76,
Organism=Caenorhabditis elegans, GI115533560, Length=544, Percent_Identity=29.4117647058824, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI115533558, Length=544, Percent_Identity=29.4117647058824, Blast_Score=230, Evalue=1e-60,
Organism=Caenorhabditis elegans, GI71987012, Length=460, Percent_Identity=31.9565217391304, Blast_Score=222, Evalue=4e-58,
Organism=Saccharomyces cerevisiae, GI6319602, Length=477, Percent_Identity=36.0587002096436, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6323537, Length=460, Percent_Identity=27.8260869565217, Blast_Score=192, Evalue=1e-49,
Organism=Saccharomyces cerevisiae, GI6323917, Length=457, Percent_Identity=26.9146608315098, Blast_Score=177, Evalue=4e-45,
Organism=Saccharomyces cerevisiae, GI6320279, Length=391, Percent_Identity=27.1099744245524, Blast_Score=167, Evalue=3e-42,
Organism=Drosophila melanogaster, GI19920676, Length=502, Percent_Identity=38.0478087649402, Blast_Score=311, Evalue=6e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: 2.4.1.15 [C]

Molecular weight: Translated: 55368; Mature: 55236

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAKGLIVVSNRLPVVLSRNEDGWGITTGAGGLVSAMAPVLRDRGGTWVGWTGASGEHE
CCCCCCEEEEECCCCEEEECCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCHH
AESLLGEFSREAGYSLKPVRLEKEDVADFYYGFANEVIWPLFHDFQSLCNFFPKYWKSYL
HHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVNELYARAVMDSGDPEAYVWVHDYHLMHVGEMLKSMGSRRRAGFFLHIPFPPMDNYLKL
HHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
PWRRRLLSALLEYDLVGFQTYRDRRNFMQSVQVLLPHVKPQGRGPVIELLLDGRKVRLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEE
FPISIDYRSFVRTARSPAVSGRVNELKSAMDGRRMLLAVDRLDYSKGITFRLEAFREALE
EEEEECHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEEHHCCCCCCEEEHHHHHHHHH
KYPGLRGNVTLMQIMVPSRQEVPRYNALKQEIERLVGEINGLFSRPGWNPVQYQYRSLGR
HCCCCCCCEEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCH
EELVAYYRAASMAIVTPLRDGMNLVAKEYCACNVNRRGVLLLSEFAGAAAQLQNGAVLVN
HHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHCCCEEEEC
PFDREGMAQSIKEALDMPAEEARRRMDRMRDVIRKNDIFRWVDMFLLAAFSRHLDDFPPT
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
YARK
HCCC
>Mature Secondary Structure 
TPAKGLIVVSNRLPVVLSRNEDGWGITTGAGGLVSAMAPVLRDRGGTWVGWTGASGEHE
CCCCCEEEEECCCCEEEECCCCCCEEECCCCHHHHHHHHHHHCCCCCEEEECCCCCCHH
AESLLGEFSREAGYSLKPVRLEKEDVADFYYGFANEVIWPLFHDFQSLCNFFPKYWKSYL
HHHHHHHHHHHCCCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVNELYARAVMDSGDPEAYVWVHDYHLMHVGEMLKSMGSRRRAGFFLHIPFPPMDNYLKL
HHHHHHHHHHHCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCC
PWRRRLLSALLEYDLVGFQTYRDRRNFMQSVQVLLPHVKPQGRGPVIELLLDGRKVRLGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCEEEEEE
FPISIDYRSFVRTARSPAVSGRVNELKSAMDGRRMLLAVDRLDYSKGITFRLEAFREALE
EEEEECHHHHHHHHCCCCCCCHHHHHHHHHCCCEEEEEEEHHCCCCCCEEEHHHHHHHHH
KYPGLRGNVTLMQIMVPSRQEVPRYNALKQEIERLVGEINGLFSRPGWNPVQYQYRSLGR
HCCCCCCCEEEEEEECCCHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCH
EELVAYYRAASMAIVTPLRDGMNLVAKEYCACNVNRRGVLLLSEFAGAAAQLQNGAVLVN
HHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHCCHHHHHCCCEEEEC
PFDREGMAQSIKEALDMPAEEARRRMDRMRDVIRKNDIFRWVDMFLLAAFSRHLDDFPPT
CCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
YARK
HCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Monovalent cations [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: D-Glucose 6-phosphate; UDPglucose [C]

Specific reaction: D-Glucose 6-phosphate + UDPglucose --> H+ + alpha,alpha'-Trehalose 6-phosphate + UDP [C]

General reaction: Hexosyl group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA