Click here to switch to the map view.

The map label for this gene is glyS

Identifier: 78357076

GI number: 78357076

Start: 2073229

End: 2075319

Strand: Reverse

Name: glyS

Synonym: Dde_2033

Alternate gene names: 78357076

Gene position: 2075319-2073229 (Counterclockwise)

Preceding gene: 78357077

Following gene: 78357074

Centisome position: 55.64

GC content: 57.05

Gene sequence:

>2091_bases
ATGTCTGTTTTCGTGATGGAGATTGGAACAGAAGAACTGCCTGCCCGTTTCCTTTCCGGACTGGAGCAGGAGATTGCTTC
GCGGTTTGAGGCTGCGCTTGCAGCAGCCAATGTGGAATTCGGTACTCTGAATGTGCAGTCGACCCCGCGCAGGCTGGCTC
TGACCATTGAAAATATTGATGCCGTGCAGCGCGAAGCTGAAGAAGTAGTTTCCGGTCCGCCCGTGCGTATAGCTTTTGAC
GCACAAGGCCGGCCTACCAAGGCGGCCGAAGGATTTGCCAGAACGCAGGGCGCTGAGCTGGACGAGCTTTTCACGCTGAC
CACCGATAAAGGTGAATACCTTGCGGTGCGCAAGAAAACCGGCGGCCGTCCCGTACAGGAAATTCTGTCAGAGGTGGCCC
CCGAGATCATTGCAGCTTTACCTTTCCCTAAAAAAATGAAGTGGGGGAGCGGCGATTTTACGTTCGGCCGTCCTCTGCGC
TGGGTGCTGGCCCTCTTTGACAGTGAAGTAGTATCGTTCACAGTGGCGGGGATTGTTTCCGGCAACGAAACCTGCGGGCA
CAGGGTTCATGGTCCGGGGCCTTTTGAGGTTTCCGGAGCAGACGCCTATGCCGGTACCGTGCGCGATAAGTGCCATGTCA
CCTTGTGCGCTGCCGACCGGCGCCGCGTGACCGTAGAAGGTGGAGACGCCCTTGCCGCACAGGCCGGTGGCCGTATCCTC
TGGAAAGACTCCCTGCTGGACGAAGTACAGGGCCTTTGCGAGCACCCTGTTCCCTGCCTGGGAGATTTTGATCCATCTTT
TCTGGAACTGCCGGCAGAGGTTCTGCTGACCAGTATGGAAAGCCATCAGAAGAGCTTTGGCGTTGCCGCTGCCGATGGCA
GCCTGATGCCGCACTTTCTGACTGTGCTCAACATGACGCCGCAGAATTCCGCGCTGGTTAAAAAAGGCTGGGAGCGGGTG
TTGCGTGCACGTCTTGAAGATGCCCGTTTCTTCTGGAAGGCGGACCTTGCTTCCGATTTTGACAGCTGGCTTGCCAAGCT
GGACAACGTCATCTTTCTTGCTCCGCTGGGCAGCATGGGTGACAAAACGCGCCGGCTGGAAAGGCTGTGCCGCTGGCTGG
CTGCCGCCGTGGCTCCTGATATTCAGGAACAGGCCGCCCGTGCAGGCCGGTTGTCCAAGGCTGATCTTGTCTCGGAAATG
GTGTACGAGTTTGATTCGCTGCAGGGGATCATGGGCGGCATTTACGCACGCCGGAAAGGAGAGGACGAGATTGTCGCCCA
GGCTGTCAGCGAGCAGTACCTGCCGGCCGGTCCCGAAAGCCCTGTTCCTGATTCTTTGTGCGGTGCGCTGCTTTCCATAG
CTGACAAGGCTGATACGCTGGCTGGCTGTTTCGGACTGTCCATGATTCCCACAGGTGCGGCCGATCCGTATGCATTGCGG
CGTTGCGCGCTGGGTATTGCGCGTATTATGCTGGAAAAAGGTCTTCGTTTCGATGTACGCGAGCTTTTCAGATACGCGCA
GGAAGGCTACGGTGAACGTCAATGGAAGCTGAAACCTGAGCAGGCAGCCGAAAAGCTGCATGAATTCTTTGTGTTGCGTC
TGAAAAACCTGTTTGTGTCACAAGGGCATGAAACGCTTCTTGTGGAAGCCGCGCTGCATGCCGGTGCCGACGATGTATGG
GCCGCAGGTGCCCGGCTGGAGGCTCTGGACCGTTTCAGCAAATCCGCAGAATTCGGTCAGGCGGTGCTTACATTCAAGCG
TGCGGCGAATATTATCCGCAAGCAGGGGCAGGAGGAAGACGGCGGACTGGATGGTTGCTACGATGCTTCGTTGTTTGAGG
ACGAAGCTGAAAAGGCGCTGGCCGGTGCGCTGGAAGACATCGCCCCTGATTTTGAAGCACGGTGGGCCGCGGATGATTAC
CATGCGCTGTTTGGTCTGCTGGGTGTGCTTCGTCCGGCCGTGGATGCTTTTTTTGACAGCGTCATGGTCATGTGCGAAGA
TCCGCATATCAGACGTAACAGGTTGAATCTGCTGCAGTCTCTTGTGCAGCGTCTTGGGCGTCTTGCTGATTTCGGTGCAT
TGCAGATGTAA

Upstream 100 bases:

>100_bases
TGCTTGCTTCTTCCGTGGCGCGGCTGTATGCCGCACAGCGTGAGGAAATGGGCTATCCCATGCTCAATACCGGAATCAGG
AAAGGATAGGGGAGTCATAT

Downstream 100 bases:

>100_bases
CGGTGGCAAGGCAAAAAATTGCCGGAACAGCCGGGAAAAAATAGTCTGTCAGGGTGTAAACAGCCAATGAAAATGTCACC
TTTTTGGGACGGTTTGGGAC

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS

Number of amino acids: Translated: 696; Mature: 695

Protein sequence:

>696_residues
MSVFVMEIGTEELPARFLSGLEQEIASRFEAALAAANVEFGTLNVQSTPRRLALTIENIDAVQREAEEVVSGPPVRIAFD
AQGRPTKAAEGFARTQGAELDELFTLTTDKGEYLAVRKKTGGRPVQEILSEVAPEIIAALPFPKKMKWGSGDFTFGRPLR
WVLALFDSEVVSFTVAGIVSGNETCGHRVHGPGPFEVSGADAYAGTVRDKCHVTLCAADRRRVTVEGGDALAAQAGGRIL
WKDSLLDEVQGLCEHPVPCLGDFDPSFLELPAEVLLTSMESHQKSFGVAAADGSLMPHFLTVLNMTPQNSALVKKGWERV
LRARLEDARFFWKADLASDFDSWLAKLDNVIFLAPLGSMGDKTRRLERLCRWLAAAVAPDIQEQAARAGRLSKADLVSEM
VYEFDSLQGIMGGIYARRKGEDEIVAQAVSEQYLPAGPESPVPDSLCGALLSIADKADTLAGCFGLSMIPTGAADPYALR
RCALGIARIMLEKGLRFDVRELFRYAQEGYGERQWKLKPEQAAEKLHEFFVLRLKNLFVSQGHETLLVEAALHAGADDVW
AAGARLEALDRFSKSAEFGQAVLTFKRAANIIRKQGQEEDGGLDGCYDASLFEDEAEKALAGALEDIAPDFEARWAADDY
HALFGLLGVLRPAVDAFFDSVMVMCEDPHIRRNRLNLLQSLVQRLGRLADFGALQM

Sequences:

>Translated_696_residues
MSVFVMEIGTEELPARFLSGLEQEIASRFEAALAAANVEFGTLNVQSTPRRLALTIENIDAVQREAEEVVSGPPVRIAFD
AQGRPTKAAEGFARTQGAELDELFTLTTDKGEYLAVRKKTGGRPVQEILSEVAPEIIAALPFPKKMKWGSGDFTFGRPLR
WVLALFDSEVVSFTVAGIVSGNETCGHRVHGPGPFEVSGADAYAGTVRDKCHVTLCAADRRRVTVEGGDALAAQAGGRIL
WKDSLLDEVQGLCEHPVPCLGDFDPSFLELPAEVLLTSMESHQKSFGVAAADGSLMPHFLTVLNMTPQNSALVKKGWERV
LRARLEDARFFWKADLASDFDSWLAKLDNVIFLAPLGSMGDKTRRLERLCRWLAAAVAPDIQEQAARAGRLSKADLVSEM
VYEFDSLQGIMGGIYARRKGEDEIVAQAVSEQYLPAGPESPVPDSLCGALLSIADKADTLAGCFGLSMIPTGAADPYALR
RCALGIARIMLEKGLRFDVRELFRYAQEGYGERQWKLKPEQAAEKLHEFFVLRLKNLFVSQGHETLLVEAALHAGADDVW
AAGARLEALDRFSKSAEFGQAVLTFKRAANIIRKQGQEEDGGLDGCYDASLFEDEAEKALAGALEDIAPDFEARWAADDY
HALFGLLGVLRPAVDAFFDSVMVMCEDPHIRRNRLNLLQSLVQRLGRLADFGALQM
>Mature_695_residues
SVFVMEIGTEELPARFLSGLEQEIASRFEAALAAANVEFGTLNVQSTPRRLALTIENIDAVQREAEEVVSGPPVRIAFDA
QGRPTKAAEGFARTQGAELDELFTLTTDKGEYLAVRKKTGGRPVQEILSEVAPEIIAALPFPKKMKWGSGDFTFGRPLRW
VLALFDSEVVSFTVAGIVSGNETCGHRVHGPGPFEVSGADAYAGTVRDKCHVTLCAADRRRVTVEGGDALAAQAGGRILW
KDSLLDEVQGLCEHPVPCLGDFDPSFLELPAEVLLTSMESHQKSFGVAAADGSLMPHFLTVLNMTPQNSALVKKGWERVL
RARLEDARFFWKADLASDFDSWLAKLDNVIFLAPLGSMGDKTRRLERLCRWLAAAVAPDIQEQAARAGRLSKADLVSEMV
YEFDSLQGIMGGIYARRKGEDEIVAQAVSEQYLPAGPESPVPDSLCGALLSIADKADTLAGCFGLSMIPTGAADPYALRR
CALGIARIMLEKGLRFDVRELFRYAQEGYGERQWKLKPEQAAEKLHEFFVLRLKNLFVSQGHETLLVEAALHAGADDVWA
AGARLEALDRFSKSAEFGQAVLTFKRAANIIRKQGQEEDGGLDGCYDASLFEDEAEKALAGALEDIAPDFEARWAADDYH
ALFGLLGVLRPAVDAFFDSVMVMCEDPHIRRNRLNLLQSLVQRLGRLADFGALQM

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Escherichia coli, GI1789982, Length=694, Percent_Identity=36.8876080691643, Blast_Score=420, Evalue=1e-118,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYGB_DESDG (Q30ZR6)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388525.1
- ProteinModelPortal:   Q30ZR6
- STRING:   Q30ZR6
- GeneID:   3757041
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_2033
- NMPDR:   fig|207559.3.peg.2316
- eggNOG:   COG0751
- HOGENOM:   HBG586599
- OMA:   FIRPVHT
- ProtClustDB:   PRK01233
- BioCyc:   DDES207559:DDE_2033-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00255
- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311
- PRINTS:   PR01045
- TIGRFAMs:   TIGR00211

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f

EC number: =6.1.1.14

Molecular weight: Translated: 76084; Mature: 75952

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVFVMEIGTEELPARFLSGLEQEIASRFEAALAAANVEFGTLNVQSTPRRLALTIENID
CEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHH
AVQREAEEVVSGPPVRIAFDAQGRPTKAAEGFARTQGAELDELFTLTTDKGEYLAVRKKT
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHEEEECCCCCEEEEECCC
GGRPVQEILSEVAPEIIAALPFPKKMKWGSGDFTFGRPLRWVLALFDSEVVSFTVAGIVS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEC
GNETCGHRVHGPGPFEVSGADAYAGTVRDKCHVTLCAADRRRVTVEGGDALAAQAGGRIL
CCCCCCCCCCCCCCCEECCCCHHHCCCCCCEEEEEEECCCCEEEECCCCCHHHHCCCEEE
WKDSLLDEVQGLCEHPVPCLGDFDPSFLELPAEVLLTSMESHQKSFGVAAADGSLMPHFL
EHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCEECCCCHHHHHH
TVLNMTPQNSALVKKGWERVLRARLEDARFFWKADLASDFDSWLAKLDNVIFLAPLGSMG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DKTRRLERLCRWLAAAVAPDIQEQAARAGRLSKADLVSEMVYEFDSLQGIMGGIYARRKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EDEIVAQAVSEQYLPAGPESPVPDSLCGALLSIADKADTLAGCFGLSMIPTGAADPYALR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
RCALGIARIMLEKGLRFDVRELFRYAQEGYGERQWKLKPEQAAEKLHEFFVLRLKNLFVS
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGHETLLVEAALHAGADDVWAAGARLEALDRFSKSAEFGQAVLTFKRAANIIRKQGQEED
CCCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGLDGCYDASLFEDEAEKALAGALEDIAPDFEARWAADDYHALFGLLGVLRPAVDAFFDS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VMVMCEDPHIRRNRLNLLQSLVQRLGRLADFGALQM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
SVFVMEIGTEELPARFLSGLEQEIASRFEAALAAANVEFGTLNVQSTPRRLALTIENID
EEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEEEEEHHHH
AVQREAEEVVSGPPVRIAFDAQGRPTKAAEGFARTQGAELDELFTLTTDKGEYLAVRKKT
HHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCHHHHEEEECCCCCEEEEECCC
GGRPVQEILSEVAPEIIAALPFPKKMKWGSGDFTFGRPLRWVLALFDSEVVSFTVAGIVS
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHEEC
GNETCGHRVHGPGPFEVSGADAYAGTVRDKCHVTLCAADRRRVTVEGGDALAAQAGGRIL
CCCCCCCCCCCCCCCEECCCCHHHCCCCCCEEEEEEECCCCEEEECCCCCHHHHCCCEEE
WKDSLLDEVQGLCEHPVPCLGDFDPSFLELPAEVLLTSMESHQKSFGVAAADGSLMPHFL
EHHHHHHHHHHHHCCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCEECCCCHHHHHH
TVLNMTPQNSALVKKGWERVLRARLEDARFFWKADLASDFDSWLAKLDNVIFLAPLGSMG
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCC
DKTRRLERLCRWLAAAVAPDIQEQAARAGRLSKADLVSEMVYEFDSLQGIMGGIYARRKG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EDEIVAQAVSEQYLPAGPESPVPDSLCGALLSIADKADTLAGCFGLSMIPTGAADPYALR
HHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHH
RCALGIARIMLEKGLRFDVRELFRYAQEGYGERQWKLKPEQAAEKLHEFFVLRLKNLFVS
HHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QGHETLLVEAALHAGADDVWAAGARLEALDRFSKSAEFGQAVLTFKRAANIIRKQGQEED
CCCHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
GGLDGCYDASLFEDEAEKALAGALEDIAPDFEARWAADDYHALFGLLGVLRPAVDAFFDS
CCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VMVMCEDPHIRRNRLNLLQSLVQRLGRLADFGALQM
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA