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The map label for this gene is tilS [H]

Identifier: 78357070

GI number: 78357070

Start: 2066282

End: 2067355

Strand: Direct

Name: tilS [H]

Synonym: Dde_2027

Alternate gene names: 78357070

Gene position: 2066282-2067355 (Clockwise)

Preceding gene: 78357069

Following gene: 78357071

Centisome position: 55.39

GC content: 59.4

Gene sequence:

>1074_bases
ATGCCGTACCCGCCACTGCCTAACCAGCTTCCTGCAAGGCTGCAGGATATCCCCGCGCCGCAGGCACGTCTGTGTCTGGA
TGTCGGGCGTTTTATGACGGAACAGCTTGCCGGTGCCGGCTGTATTTCCCGCGAGCAAGCCCTGAGCAGTGCCCCCCTGA
ACGGATATACGATGCTGGCGGCTTGTTCCGGGGGCGCCGACTCCACAGCACTGCTGCTCATCCTGCATTATCTGGCTCCG
CGCATGGGACATACCGTAGTTGCCGTTCACCTTGATCATGCGCTGCGACAGGAAAGCGCAGACGACGCGCACTTTGTCAG
TCAGCTCTGCTCCGCCGCCGGCATCAGGCTTTTTTCAGTCCGTACAGACGTTGCGGATCTGGCGGCCGCCGGACGCTGTG
GTCTGGAGGAAGCAGGCCGCAAAGCACGTTATGCCTTTTACGCGAAAACAGCCGCCCTTTTTTCCCGCCCTGTCATATGT
ACCGGCCACCATGCGGATGATCTTAAGGAAGACGTGCTCATGCGCCTTATCCGCGGTGCAGGCTGGCCGGCACTCGGCGG
AATGCAGGCCGTCATTCCCGAACGGAGCCTGCTGCGCCCCCTGCTTGTGACAGAAAAGTACAAGCTGACAACTTTTCTGG
AAGCATGCCGGTGCCCATGGCGCGAAGATGCTTCAAATTCTGACCCGTCGTTCACCCGCAACCGCATCCGCAACACCATC
ATTCCCCTGTTGCAGCAGGAAAATCCTTCTTTCGGAACCACAATCACAGAACTGTGGGAAGCCGCAGCCGCTGACAGAGA
CTACTGGACAGACGTGCTGCCCGAACTGGCACCGGACAAAAACGGCCAGATTTTCATTGCCCGCAGCACACTGCAGGGTC
TGCACAAAGCAGGCCGGCTGCGGCTGTACAAAAAAGCTCTGGAAGCTCTGGGCGCCGGACAGCCGCTGCATGCCTCCATC
ATGGCGCTGGAACATGCCTGGCACAGAAATCAGGGCGGCAAGTGCATTCAGTTTCCCGGCGGAAAAACAGCCAGGATACG
CCATGGCGGCATATGCTTCTGCCTAACCGCCTGA

Upstream 100 bases:

>100_bases
CTATCTTGACTCTTTGCTGTCAGAAAACGGCATCGCCATCTGAACGGGACAGCCCTTTTCCGCCGTTATACCGCGTTAAA
GGGCTGCCGCCCACCCTGCC

Downstream 100 bases:

>100_bases
TTCTTTTTTATCCGATTGACAGGGTGCGATTCTTCGCAGTATGACTTCGTGAAATAAATGACAAGGAGGAATGACGTGAA
TATACTCATCTTCGGCCCCA

Product: ketose-bisphosphate aldolase, class-II

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MPYPPLPNQLPARLQDIPAPQARLCLDVGRFMTEQLAGAGCISREQALSSAPLNGYTMLAACSGGADSTALLLILHYLAP
RMGHTVVAVHLDHALRQESADDAHFVSQLCSAAGIRLFSVRTDVADLAAAGRCGLEEAGRKARYAFYAKTAALFSRPVIC
TGHHADDLKEDVLMRLIRGAGWPALGGMQAVIPERSLLRPLLVTEKYKLTTFLEACRCPWREDASNSDPSFTRNRIRNTI
IPLLQQENPSFGTTITELWEAAAADRDYWTDVLPELAPDKNGQIFIARSTLQGLHKAGRLRLYKKALEALGAGQPLHASI
MALEHAWHRNQGGKCIQFPGGKTARIRHGGICFCLTA

Sequences:

>Translated_357_residues
MPYPPLPNQLPARLQDIPAPQARLCLDVGRFMTEQLAGAGCISREQALSSAPLNGYTMLAACSGGADSTALLLILHYLAP
RMGHTVVAVHLDHALRQESADDAHFVSQLCSAAGIRLFSVRTDVADLAAAGRCGLEEAGRKARYAFYAKTAALFSRPVIC
TGHHADDLKEDVLMRLIRGAGWPALGGMQAVIPERSLLRPLLVTEKYKLTTFLEACRCPWREDASNSDPSFTRNRIRNTI
IPLLQQENPSFGTTITELWEAAAADRDYWTDVLPELAPDKNGQIFIARSTLQGLHKAGRLRLYKKALEALGAGQPLHASI
MALEHAWHRNQGGKCIQFPGGKTARIRHGGICFCLTA
>Mature_356_residues
PYPPLPNQLPARLQDIPAPQARLCLDVGRFMTEQLAGAGCISREQALSSAPLNGYTMLAACSGGADSTALLLILHYLAPR
MGHTVVAVHLDHALRQESADDAHFVSQLCSAAGIRLFSVRTDVADLAAAGRCGLEEAGRKARYAFYAKTAALFSRPVICT
GHHADDLKEDVLMRLIRGAGWPALGGMQAVIPERSLLRPLLVTEKYKLTTFLEACRCPWREDASNSDPSFTRNRIRNTII
PLLQQENPSFGTTITELWEAAAADRDYWTDVLPELAPDKNGQIFIARSTLQGLHKAGRLRLYKKALEALGAGQPLHASIM
ALEHAWHRNQGGKCIQFPGGKTARIRHGGICFCLTA

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=207, Percent_Identity=32.3671497584541, Blast_Score=92, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 38939; Mature: 38808

Theoretical pI: Translated: 8.49; Mature: 8.49

Prosite motif: PS00602 ALDOLASE_CLASS_II_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPYPPLPNQLPARLQDIPAPQARLCLDVGRFMTEQLAGAGCISREQALSSAPLNGYTMLA
CCCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEE
ACSGGADSTALLLILHYLAPRMGHTVVAVHLDHALRQESADDAHFVSQLCSAAGIRLFSV
EECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHCCEEEEE
RTDVADLAAAGRCGLEEAGRKARYAFYAKTAALFSRPVICTGHHADDLKEDVLMRLIRGA
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCC
GWPALGGMQAVIPERSLLRPLLVTEKYKLTTFLEACRCPWREDASNSDPSFTRNRIRNTI
CCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IPLLQQENPSFGTTITELWEAAAADRDYWTDVLPELAPDKNGQIFIARSTLQGLHKAGRL
HHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHH
RLYKKALEALGAGQPLHASIMALEHAWHRNQGGKCIQFPGGKTARIRHGGICFCLTA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCEEEEEEC
>Mature Secondary Structure 
PYPPLPNQLPARLQDIPAPQARLCLDVGRFMTEQLAGAGCISREQALSSAPLNGYTMLA
CCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCCEEEEE
ACSGGADSTALLLILHYLAPRMGHTVVAVHLDHALRQESADDAHFVSQLCSAAGIRLFSV
EECCCCCHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHCCCCHHHHHHHHHHHHCCEEEEE
RTDVADLAAAGRCGLEEAGRKARYAFYAKTAALFSRPVICTGHHADDLKEDVLMRLIRGA
HHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHCC
GWPALGGMQAVIPERSLLRPLLVTEKYKLTTFLEACRCPWREDASNSDPSFTRNRIRNTI
CCCCCCCCHHHCCCHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHH
IPLLQQENPSFGTTITELWEAAAADRDYWTDVLPELAPDKNGQIFIARSTLQGLHKAGRL
HHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHHH
RLYKKALEALGAGQPLHASIMALEHAWHRNQGGKCIQFPGGKTARIRHGGICFCLTA
HHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEECCCCCEEEEECCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA