Click here to switch to the map view.

The map label for this gene is gluQ [H]

Identifier: 78357047

GI number: 78357047

Start: 2045979

End: 2046968

Strand: Reverse

Name: gluQ [H]

Synonym: Dde_2004

Alternate gene names: 78357047

Gene position: 2046968-2045979 (Counterclockwise)

Preceding gene: 78357049

Following gene: 78357046

Centisome position: 54.88

GC content: 57.58

Gene sequence:

>990_bases
ATGGGCGTTCGGGGACGGCTGGCTCCCAGTCCGACAGGGTATCTTCATATAGGAAACGCATGGGCTTTTCTGATCGCATG
GCTTGCCGTGCGGGCTGAGGGGGGAAGTCTTGTTCTGCGCATGGAAGATATCGACCCTGACCGGTCGCGCAGGGAATTTG
AGCTTGGAATTGTAGAGGACCTTAAGTGGCTGGGCCTTGACTGGGACGAGGGCCCCGATAGCGGTGGGGCCTTTGGCCCC
TACAGGCAGAGTGACAGGCTGGGGTATTACGGTCAGGTGCTTGATGAATTTATCCGCAGCGGTAAAGTGTATCCGTGTTA
CTGCACCCGTAAGGAACTACGGTCCATGGCGTCTGCTCCGCATCTGGGGGAGCATGTTCCTGTTTATCCGGGAACATGCC
GCAACCTTGATGCAGCCGGGCAACGGCACCGTGAAGCTTCCGGGCGGGCACCTGCACAGCGTATGCGCTGTGGGGAGAAG
CCGCGGACTTTTGTGGATGCAGTGTACGGCAGGCAGAGATATGTTCCTGCTGAATGGGGCGGGGATTTTCCTGTCCGCCG
GTCTGACGGTGTTTATGCATATCAGTTTGCCGTTGTGCTGGATGATGCCGCAATGCAGGTCAATCAGGTCGTCCGCGGGC
GTGATATTCTGCATTGTACGCCGCGGCAGCTTGTTATTTATGAGGCCATAGGAGCCGAACCGCCGGCATATATTCATGTA
CCCCTTGTCCTTGATGCGGAGGGAGAAAGGCTGGCCAAGCGTCACGGTCACTTCGAACTGCGCAGGCTGCGTGAAGCAGG
CGTTGCACCGCAGGCGCTTGTCGGTTTTTTAGGGTGGAAAGCCGGTTTGCTGGAAACCTGTGTGCCATGCCCGCCTGACC
TGCTTGCGGCCCGCTTCCGGAAAGAGAATCTGCCACATGAGGATATCCGGCTCGATCATGATATCCTTTCGCTGCTTGCC
GGTATATCGGAGCAGGGTTCTCTTGCATAG

Upstream 100 bases:

>100_bases
CTTGTCAAGTTTGCTGAAATACCGGCAAGAGCGTAAGCAGATTCTGCTTCAGGCGAGCGGTTGCTTTAATTGTTAACACA
GAGATGTAAAAGAGGCTGTT

Downstream 100 bases:

>100_bases
TCTGATGGTTACATGGTAGGCGGGATGTCCGGATATCTGCTCCGGTATTATTGCAAGGAAGGTATGCTATGATTGAAAAT
GCACAAGCTGTTTTTACTGC

Product: glutamyl-tRNA synthetase

Products: AMP; diphosphate; L-glutamyl-tRNA(Glu)

Alternate protein names: Glu-Q-RSs [H]

Number of amino acids: Translated: 329; Mature: 328

Protein sequence:

>329_residues
MGVRGRLAPSPTGYLHIGNAWAFLIAWLAVRAEGGSLVLRMEDIDPDRSRREFELGIVEDLKWLGLDWDEGPDSGGAFGP
YRQSDRLGYYGQVLDEFIRSGKVYPCYCTRKELRSMASAPHLGEHVPVYPGTCRNLDAAGQRHREASGRAPAQRMRCGEK
PRTFVDAVYGRQRYVPAEWGGDFPVRRSDGVYAYQFAVVLDDAAMQVNQVVRGRDILHCTPRQLVIYEAIGAEPPAYIHV
PLVLDAEGERLAKRHGHFELRRLREAGVAPQALVGFLGWKAGLLETCVPCPPDLLAARFRKENLPHEDIRLDHDILSLLA
GISEQGSLA

Sequences:

>Translated_329_residues
MGVRGRLAPSPTGYLHIGNAWAFLIAWLAVRAEGGSLVLRMEDIDPDRSRREFELGIVEDLKWLGLDWDEGPDSGGAFGP
YRQSDRLGYYGQVLDEFIRSGKVYPCYCTRKELRSMASAPHLGEHVPVYPGTCRNLDAAGQRHREASGRAPAQRMRCGEK
PRTFVDAVYGRQRYVPAEWGGDFPVRRSDGVYAYQFAVVLDDAAMQVNQVVRGRDILHCTPRQLVIYEAIGAEPPAYIHV
PLVLDAEGERLAKRHGHFELRRLREAGVAPQALVGFLGWKAGLLETCVPCPPDLLAARFRKENLPHEDIRLDHDILSLLA
GISEQGSLA
>Mature_328_residues
GVRGRLAPSPTGYLHIGNAWAFLIAWLAVRAEGGSLVLRMEDIDPDRSRREFELGIVEDLKWLGLDWDEGPDSGGAFGPY
RQSDRLGYYGQVLDEFIRSGKVYPCYCTRKELRSMASAPHLGEHVPVYPGTCRNLDAAGQRHREASGRAPAQRMRCGEKP
RTFVDAVYGRQRYVPAEWGGDFPVRRSDGVYAYQFAVVLDDAAMQVNQVVRGRDILHCTPRQLVIYEAIGAEPPAYIHVP
LVLDAEGERLAKRHGHFELRRLREAGVAPQALVGFLGWKAGLLETCVPCPPDLLAARFRKENLPHEDIRLDHDILSLLAG
ISEQGSLA

Specific function: Catalyzes the tRNA-independent activation of glutamate in presence of ATP and the subsequent transfer of glutamate onto a tRNA(Asp). Glutamate is transferred on the 2-amino-5-(4,5- dihydroxy-2-cyclopenten-1-yl) moiety of the queuosine in the wobble positi

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family. GluQ subfamily [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=277, Percent_Identity=35.0180505415162, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1788743, Length=285, Percent_Identity=35.7894736842105, Blast_Score=187, Evalue=6e-49,
Organism=Escherichia coli, GI226510925, Length=251, Percent_Identity=33.8645418326693, Blast_Score=123, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71993394, Length=277, Percent_Identity=31.7689530685921, Blast_Score=154, Evalue=4e-38,
Organism=Saccharomyces cerevisiae, GI6324540, Length=287, Percent_Identity=32.404181184669, Blast_Score=155, Evalue=8e-39,
Organism=Saccharomyces cerevisiae, GI6324742, Length=115, Percent_Identity=28.695652173913, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24665321, Length=296, Percent_Identity=35.472972972973, Blast_Score=191, Evalue=8e-49,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR022380
- InterPro:   IPR000924
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: 6.1.1.17

Molecular weight: Translated: 36667; Mature: 36536

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGVRGRLAPSPTGYLHIGNAWAFLIAWLAVRAEGGSLVLRMEDIDPDRSRREFELGIVED
CCCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEECCHHHH
LKWLGLDWDEGPDSGGAFGPYRQSDRLGYYGQVLDEFIRSGKVYPCYCTRKELRSMASAP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCC
HLGEHVPVYPGTCRNLDAAGQRHREASGRAPAQRMRCGEKPRTFVDAVYGRQRYVPAEWG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCCEECCCCCC
GDFPVRRSDGVYAYQFAVVLDDAAMQVNQVVRGRDILHCTPRQLVIYEAIGAEPPAYIHV
CCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCEEEE
PLVLDAEGERLAKRHGHFELRRLREAGVAPQALVGFLGWKAGLLETCVPCPPDLLAARFR
EEEEECCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KENLPHEDIRLDHDILSLLAGISEQGSLA
HCCCCHHHHHCCHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
GVRGRLAPSPTGYLHIGNAWAFLIAWLAVRAEGGSLVLRMEDIDPDRSRREFELGIVED
CCCCCCCCCCCCEEEECHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCCEEECCHHHH
LKWLGLDWDEGPDSGGAFGPYRQSDRLGYYGQVLDEFIRSGKVYPCYCTRKELRSMASAP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCC
HLGEHVPVYPGTCRNLDAAGQRHREASGRAPAQRMRCGEKPRTFVDAVYGRQRYVPAEWG
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCCCHHHHHHHHCCCEECCCCCC
GDFPVRRSDGVYAYQFAVVLDDAAMQVNQVVRGRDILHCTPRQLVIYEAIGAEPPAYIHV
CCCCCCCCCCEEEEEEEEEHHHHHHHHHHHHCCCCEEEECCCCEEEEEECCCCCCCEEEE
PLVLDAEGERLAKRHGHFELRRLREAGVAPQALVGFLGWKAGLLETCVPCPPDLLAARFR
EEEEECCCHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
KENLPHEDIRLDHDILSLLAGISEQGSLA
HCCCCHHHHHCCHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-glutamate; tRNA(Glu)

Specific reaction: ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)

General reaction: Aminoacylation; Esterification [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA