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The map label for this gene is tpiA

Identifier: 78357032

GI number: 78357032

Start: 2032369

End: 2033121

Strand: Reverse

Name: tpiA

Synonym: Dde_1989

Alternate gene names: 78357032

Gene position: 2033121-2032369 (Counterclockwise)

Preceding gene: 78357033

Following gene: 78357031

Centisome position: 54.5

GC content: 55.78

Gene sequence:

>753_bases
ATGAAAAAACTGATGGCTGCAAACTGGAAAATGTATAAGACCCCGCAGGAAGCTGCGCTTACCGCCGGAGAACTTGTAAA
AGCTGTGGGGACAGTGCCGGATGACCGTGAGGTACTTGTTTTTCCGCCTTTTGTGGGGCTTGCACAGGTTGCTCAGGCTT
TTTCCGGTGTTGCCGGTTTTTATTGCGGAGCTCAAAACGTCTATCCCGCAGATGAAGGAGCGTATACCGGCGAAATATCG
CCGGGGATGATCGTGTCTACAGGTGCCGCGTGGGCGTTGACCGGACATTCGGAGCGCCGGAGCGTTCTGGGTGAAAGCAG
TGCCTTTGTGGGGCAGAAGACCGCATTTTGTCTGGAAAAAGGCCTGAATGTGGTGCTGTGCATCGGCGAAACGCTGGAAG
AACGTGAAGGCGGGCAGCTGGAAAAGGTGCTTCGCGGACAGCTTTCCGAGGGGCTGTCCGGTGTGCCGGCCGGTATTGCT
CCTGAACGGCTGGCTATAGCTTATGAGCCTGTCTGGGCCATAGGTACAGGCAAAGTTGCCGGTACCGCCGAAATTGCCGC
AACCCACGCTGTGGTGCGCGGTATGCTGCGCGAATTGTTGCCCGCAGCGGCTGATTCCATGCGTATTCTGTACGGTGGCA
GCGTAAAGCCCGACAATGCGGCCGCTATCATTTCTCTTGACAATGTGGACGGAGTCTTGGTAGGTGGCGCTTCTTTACAG
GCTGAAAGCTTCAGCCGGATAATTCTTGCCTGA

Upstream 100 bases:

>100_bases
GGTATTATCAGGATACCGGCGAAGATGCTCTGGTTTACACGCGGCAGGTTCCGCCGCACAACGCGGAAAAACAAAGTCCG
GAACTTTAGGAGATCAGACT

Downstream 100 bases:

>100_bases
TTTCCGCCGGTGCGGAATCGCATATAGCTCCGAGGAGGAACAAGAACCGTGCAAACTGTGATTCTCACTCTCCACATCGT
CGCTTGTATCGTGTTGACGG

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MKKLMAANWKMYKTPQEAALTAGELVKAVGTVPDDREVLVFPPFVGLAQVAQAFSGVAGFYCGAQNVYPADEGAYTGEIS
PGMIVSTGAAWALTGHSERRSVLGESSAFVGQKTAFCLEKGLNVVLCIGETLEEREGGQLEKVLRGQLSEGLSGVPAGIA
PERLAIAYEPVWAIGTGKVAGTAEIAATHAVVRGMLRELLPAAADSMRILYGGSVKPDNAAAIISLDNVDGVLVGGASLQ
AESFSRIILA

Sequences:

>Translated_250_residues
MKKLMAANWKMYKTPQEAALTAGELVKAVGTVPDDREVLVFPPFVGLAQVAQAFSGVAGFYCGAQNVYPADEGAYTGEIS
PGMIVSTGAAWALTGHSERRSVLGESSAFVGQKTAFCLEKGLNVVLCIGETLEEREGGQLEKVLRGQLSEGLSGVPAGIA
PERLAIAYEPVWAIGTGKVAGTAEIAATHAVVRGMLRELLPAAADSMRILYGGSVKPDNAAAIISLDNVDGVLVGGASLQ
AESFSRIILA
>Mature_250_residues
MKKLMAANWKMYKTPQEAALTAGELVKAVGTVPDDREVLVFPPFVGLAQVAQAFSGVAGFYCGAQNVYPADEGAYTGEIS
PGMIVSTGAAWALTGHSERRSVLGESSAFVGQKTAFCLEKGLNVVLCIGETLEEREGGQLEKVLRGQLSEGLSGVPAGIA
PERLAIAYEPVWAIGTGKVAGTAEIAATHAVVRGMLRELLPAAADSMRILYGGSVKPDNAAAIISLDNVDGVLVGGASLQ
AESFSRIILA

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=252, Percent_Identity=43.6507936507937, Blast_Score=172, Evalue=2e-43,
Organism=Homo sapiens, GI4507645, Length=252, Percent_Identity=43.6507936507937, Blast_Score=172, Evalue=2e-43,
Organism=Escherichia coli, GI1790353, Length=247, Percent_Identity=38.8663967611336, Blast_Score=145, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17536593, Length=252, Percent_Identity=44.8412698412698, Blast_Score=183, Evalue=8e-47,
Organism=Saccharomyces cerevisiae, GI6320255, Length=248, Percent_Identity=39.9193548387097, Blast_Score=154, Evalue=1e-38,
Organism=Drosophila melanogaster, GI28572004, Length=250, Percent_Identity=38.8, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI28572008, Length=244, Percent_Identity=39.344262295082, Blast_Score=154, Evalue=5e-38,
Organism=Drosophila melanogaster, GI28572006, Length=244, Percent_Identity=39.344262295082, Blast_Score=154, Evalue=5e-38,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_DESDG (Q30ZW0)

Other databases:

- EMBL:   CP000112
- RefSeq:   YP_388481.1
- HSSP:   P36204
- ProteinModelPortal:   Q30ZW0
- SMR:   Q30ZW0
- STRING:   Q30ZW0
- GeneID:   3756996
- GenomeReviews:   CP000112_GR
- KEGG:   dde:Dde_1989
- NMPDR:   fig|207559.3.peg.1631
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   DDES207559:DDE_1989-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 25936; Mature: 25936

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 169-169 BINDING 8-8 BINDING 10-10

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKLMAANWKMYKTPQEAALTAGELVKAVGTVPDDREVLVFPPFVGLAQVAQAFSGVAGF
CCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
YCGAQNVYPADEGAYTGEISPGMIVSTGAAWALTGHSERRSVLGESSAFVGQKTAFCLEK
HCCCCCCCCCCCCCEECCCCCCEEEECCCEEEEECCHHHHHHHCCCCHHHCHHHHHHHHC
GLNVVLCIGETLEEREGGQLEKVLRGQLSEGLSGVPAGIAPERLAIAYEPVWAIGTGKVA
CCEEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEECCCCC
GTAEIAATHAVVRGMLRELLPAAADSMRILYGGSVKPDNAAAIISLDNVDGVLVGGASLQ
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCC
AESFSRIILA
HHHHHHEECC
>Mature Secondary Structure
MKKLMAANWKMYKTPQEAALTAGELVKAVGTVPDDREVLVFPPFVGLAQVAQAFSGVAGF
CCCCCCCCCEEECCCHHHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHHHH
YCGAQNVYPADEGAYTGEISPGMIVSTGAAWALTGHSERRSVLGESSAFVGQKTAFCLEK
HCCCCCCCCCCCCCEECCCCCCEEEECCCEEEEECCHHHHHHHCCCCHHHCHHHHHHHHC
GLNVVLCIGETLEEREGGQLEKVLRGQLSEGLSGVPAGIAPERLAIAYEPVWAIGTGKVA
CCEEEEEECCHHHHCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCEEEEECCCCC
GTAEIAATHAVVRGMLRELLPAAADSMRILYGGSVKPDNAAAIISLDNVDGVLVGGASLQ
CHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEECCCCEEEEECCCCC
AESFSRIILA
HHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA