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The map label for this gene is nadB [H]

Identifier: 78356872

GI number: 78356872

Start: 1877546

End: 1879150

Strand: Reverse

Name: nadB [H]

Synonym: Dde_1829

Alternate gene names: 78356872

Gene position: 1879150-1877546 (Counterclockwise)

Preceding gene: 78356873

Following gene: 78356863

Centisome position: 50.38

GC content: 56.2

Gene sequence:

>1605_bases
ATGTCCACTTTCCGTCCCGTCACTCCTGTTCTGGTCATCGGATCCGGTATTGCCGGATGCACTGCGGCTCTTACTCTGGC
CGACATGGGTATAGAGGTCACCCTGATAACCAGCGGTGATACGCTTGATAAAGGCAACACTGCGCTGGCACAGGGAGGCA
TTGTTTTCCGTGCGCACGACGGTGACCCCAAGCAGCTGGAAAAAGATATTCTGGTGGCCGGTCACAGGTACAATTCGACA
AAAGCCGTACGCCATGTGAGCGTTGAAGGACCTAAGGCCGTTCAGGACGTTCTTGTGGACAGGCTGTCCATTCCTTTTGC
CCGTCAGCAGGACGACAGCAAGACCGGCGCCGACTGCGAGTGGGACATGACCATGGAGGGCGGTCACGCTGCTCAGCGTA
TTCTGCATTGTGCTGATTACACCGGCCGGGCCATTATGGACGGGCTGATGCGCGAGGTGCAGGCATCACCCAATATACGG
GTGCTCACCAACCGCACCGCCATCGACCTGTTGACCAGTCATCACCACGGTACCAGTCTGGAGTTCAAATACCAGCTGGT
GAACCAGTGCGCCGGAGCATATGTTTTTAACGAACAGTTACGTTGTGTGGAGACCATTCTGGCAGATTTTACCGTGCTGG
CCACGGGAGGAATAGGACAGATTTACCTGCACACAACCAACTCTCCTGTGTGTGTCGGTTCCGGTCTGGCCATGGCATAC
AGGGCTGGTGGGCGAATTGAAAATGCAGAATTTGTTCAGTTTCATCCCACAGCGCTGTATCACCGCACTGAACGCCGTTT
TTTGATTACCGAGGCCATGCGCGGTGAGGGAGCCCGGCTTGTCAATGCGTCCGGCGAACCTTTCATGCACCGCTATGATC
CCCGGGCCGATCTTGCGCCGCGTGACATTGTGGCCCGCGCCATTGTGGAAGAAATGCTCTCCACAGGCAGCGACTGTGTT
TTTCTGGATGCGGCCAACTATGTCGAGCACAATCTTGAAACGCGGTTTCCTACCATTTTCCAGCGATGCCTGAATCTTGG
TATTGATATCCGCAGACAGCCCATTCCCGTTGTGCCCGCAGCACATTATTTCTGCGGAGGGGTGCTGGCCGACCTGAACG
GCAGGACTACGCTGGAACGCCTTTACAGTGTGGGCGAATGCAACTGCACCGGGGTACACGGCGCCAACAGACTTGCCAGC
ACTTCCCTGCTTGAAGCCCTGCTGTGGGGCCGCAACGCGGCATTGAATATTCATGGCAGACTGGGACGCCGGGCAGCGCT
GAATAAGAGGCTGCGTGATTCCATTCCTGACTGGAATCCGCTGGGCGATGAAAAAAATGATGACCCGGCCCTGATTGCTC
AGGACTGGACAACCCTGCGCAATACCATGTGGAATTACGTGGGCATTACCAGAACATCCAGCCGTCTGCAGCGTGCTCAT
GACGAGATGCGCGACCTGTGGCGTCATGTGAGTGACTTTTACAAGCGCACTCCCCTCTCCAAGCCGCTTGTGGATCTTTT
CCACGGCTGTCAGGCGGGTTACGTGGTTACCATGGCGGCACGCCGCAACAAACAGAGCGTTGGCTGCCATTACCGGATAG
ACTGA

Upstream 100 bases:

>100_bases
GCACATGTGCGCCGGTTATGGTTCCCCTAGAAATGCAGGACAACGCCCGTGTTGCCCTGGAGCGCATGCTGCAGGCCTGC
GCATAAAAAGAGGCACTTTT

Downstream 100 bases:

>100_bases
TACGCAAATAAAAACGGGACCTTCGGGTCCCGTTTTTATTTGCGTATATGCTTTGTGCTGCTGTTACAGCAGATAATCGC
GGATAATGAGAACGCCCGTG

Product: L-aspartate oxidase

Products: NA

Alternate protein names: LASPO; Quinolinate synthase B [H]

Number of amino acids: Translated: 534; Mature: 533

Protein sequence:

>534_residues
MSTFRPVTPVLVIGSGIAGCTAALTLADMGIEVTLITSGDTLDKGNTALAQGGIVFRAHDGDPKQLEKDILVAGHRYNST
KAVRHVSVEGPKAVQDVLVDRLSIPFARQQDDSKTGADCEWDMTMEGGHAAQRILHCADYTGRAIMDGLMREVQASPNIR
VLTNRTAIDLLTSHHHGTSLEFKYQLVNQCAGAYVFNEQLRCVETILADFTVLATGGIGQIYLHTTNSPVCVGSGLAMAY
RAGGRIENAEFVQFHPTALYHRTERRFLITEAMRGEGARLVNASGEPFMHRYDPRADLAPRDIVARAIVEEMLSTGSDCV
FLDAANYVEHNLETRFPTIFQRCLNLGIDIRRQPIPVVPAAHYFCGGVLADLNGRTTLERLYSVGECNCTGVHGANRLAS
TSLLEALLWGRNAALNIHGRLGRRAALNKRLRDSIPDWNPLGDEKNDDPALIAQDWTTLRNTMWNYVGITRTSSRLQRAH
DEMRDLWRHVSDFYKRTPLSKPLVDLFHGCQAGYVVTMAARRNKQSVGCHYRID

Sequences:

>Translated_534_residues
MSTFRPVTPVLVIGSGIAGCTAALTLADMGIEVTLITSGDTLDKGNTALAQGGIVFRAHDGDPKQLEKDILVAGHRYNST
KAVRHVSVEGPKAVQDVLVDRLSIPFARQQDDSKTGADCEWDMTMEGGHAAQRILHCADYTGRAIMDGLMREVQASPNIR
VLTNRTAIDLLTSHHHGTSLEFKYQLVNQCAGAYVFNEQLRCVETILADFTVLATGGIGQIYLHTTNSPVCVGSGLAMAY
RAGGRIENAEFVQFHPTALYHRTERRFLITEAMRGEGARLVNASGEPFMHRYDPRADLAPRDIVARAIVEEMLSTGSDCV
FLDAANYVEHNLETRFPTIFQRCLNLGIDIRRQPIPVVPAAHYFCGGVLADLNGRTTLERLYSVGECNCTGVHGANRLAS
TSLLEALLWGRNAALNIHGRLGRRAALNKRLRDSIPDWNPLGDEKNDDPALIAQDWTTLRNTMWNYVGITRTSSRLQRAH
DEMRDLWRHVSDFYKRTPLSKPLVDLFHGCQAGYVVTMAARRNKQSVGCHYRID
>Mature_533_residues
STFRPVTPVLVIGSGIAGCTAALTLADMGIEVTLITSGDTLDKGNTALAQGGIVFRAHDGDPKQLEKDILVAGHRYNSTK
AVRHVSVEGPKAVQDVLVDRLSIPFARQQDDSKTGADCEWDMTMEGGHAAQRILHCADYTGRAIMDGLMREVQASPNIRV
LTNRTAIDLLTSHHHGTSLEFKYQLVNQCAGAYVFNEQLRCVETILADFTVLATGGIGQIYLHTTNSPVCVGSGLAMAYR
AGGRIENAEFVQFHPTALYHRTERRFLITEAMRGEGARLVNASGEPFMHRYDPRADLAPRDIVARAIVEEMLSTGSDCVF
LDAANYVEHNLETRFPTIFQRCLNLGIDIRRQPIPVVPAAHYFCGGVLADLNGRTTLERLYSVGECNCTGVHGANRLAST
SLLEALLWGRNAALNIHGRLGRRAALNKRLRDSIPDWNPLGDEKNDDPALIAQDWTTLRNTMWNYVGITRTSSRLQRAHD
EMRDLWRHVSDFYKRTPLSKPLVDLFHGCQAGYVVTMAARRNKQSVGCHYRID

Specific function: Catalyzes the oxidation of L-aspartate to iminoaspartate [H]

COG id: COG0029

COG function: function code H; Aspartate oxidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. NadB subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=536, Percent_Identity=28.9179104477612, Blast_Score=174, Evalue=3e-43,
Organism=Escherichia coli, GI1788928, Length=527, Percent_Identity=41.5559772296015, Blast_Score=370, Evalue=1e-103,
Organism=Escherichia coli, GI1790597, Length=453, Percent_Identity=29.8013245033113, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1786942, Length=411, Percent_Identity=33.0900243309002, Blast_Score=161, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17505833, Length=499, Percent_Identity=29.2585170340681, Blast_Score=172, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17550100, Length=414, Percent_Identity=30.4347826086957, Blast_Score=168, Evalue=6e-42,
Organism=Caenorhabditis elegans, GI71986328, Length=480, Percent_Identity=26.25, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6322701, Length=532, Percent_Identity=29.3233082706767, Blast_Score=179, Evalue=8e-46,
Organism=Saccharomyces cerevisiae, GI6322416, Length=396, Percent_Identity=32.8282828282828, Blast_Score=177, Evalue=4e-45,
Organism=Drosophila melanogaster, GI17137288, Length=474, Percent_Identity=29.3248945147679, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24655642, Length=474, Percent_Identity=29.3248945147679, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24655647, Length=474, Percent_Identity=29.3248945147679, Blast_Score=175, Evalue=6e-44,
Organism=Drosophila melanogaster, GI24663005, Length=416, Percent_Identity=32.2115384615385, Blast_Score=171, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112
- InterPro:   IPR005288 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: =1.4.3.16 [H]

Molecular weight: Translated: 59081; Mature: 58950

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFRPVTPVLVIGSGIAGCTAALTLADMGIEVTLITSGDTLDKGNTALAQGGIVFRAHD
CCCCCCCCCEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCEEEEECC
GDPKQLEKDILVAGHRYNSTKAVRHVSVEGPKAVQDVLVDRLSIPFARQQDDSKTGADCE
CCHHHHHHHHEEECCCCCCCHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
WDMTMEGGHAAQRILHCADYTGRAIMDGLMREVQASPNIRVLTNRTAIDLLTSHHHGTSL
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCE
EFKYQLVNQCAGAYVFNEQLRCVETILADFTVLATGGIGQIYLHTTNSPVCVGSGLAMAY
EEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCEEECCCHHHHH
RAGGRIENAEFVQFHPTALYHRTERRFLITEAMRGEGARLVNASGEPFMHRYDPRADLAP
HCCCCCCCCEEEEECCHHHHHHCCCEEEEEHHHCCCCCEEEECCCCCCCCCCCCCCCCCH
RDIVARAIVEEMLSTGSDCVFLDAANYVEHNLETRFPTIFQRCLNLGIDIRRQPIPVVPA
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
AHYFCGGVLADLNGRTTLERLYSVGECNCTGVHGANRLASTSLLEALLWGRNAALNIHGR
HHHHHCCHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
LGRRAALNKRLRDSIPDWNPLGDEKNDDPALIAQDWTTLRNTMWNYVGITRTSSRLQRAH
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHEEECCHHHHHHHH
DEMRDLWRHVSDFYKRTPLSKPLVDLFHGCQAGYVVTMAARRNKQSVGCHYRID
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEHHCCCCCCCCEEECC
>Mature Secondary Structure 
STFRPVTPVLVIGSGIAGCTAALTLADMGIEVTLITSGDTLDKGNTALAQGGIVFRAHD
CCCCCCCCEEEECCCHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCEEEEECC
GDPKQLEKDILVAGHRYNSTKAVRHVSVEGPKAVQDVLVDRLSIPFARQQDDSKTGADCE
CCHHHHHHHHEEECCCCCCCHHEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCE
WDMTMEGGHAAQRILHCADYTGRAIMDGLMREVQASPNIRVLTNRTAIDLLTSHHHGTSL
EEEEECCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCCE
EFKYQLVNQCAGAYVFNEQLRCVETILADFTVLATGGIGQIYLHTTNSPVCVGSGLAMAY
EEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEECCCCEEEEEECCCCEEECCCHHHHH
RAGGRIENAEFVQFHPTALYHRTERRFLITEAMRGEGARLVNASGEPFMHRYDPRADLAP
HCCCCCCCCEEEEECCHHHHHHCCCEEEEEHHHCCCCCEEEECCCCCCCCCCCCCCCCCH
RDIVARAIVEEMLSTGSDCVFLDAANYVEHNLETRFPTIFQRCLNLGIDIRRQPIPVVPA
HHHHHHHHHHHHHHCCCCEEEEECHHHHHHCCCCCCHHHHHHHHHCCCCCCCCCCCCCCC
AHYFCGGVLADLNGRTTLERLYSVGECNCTGVHGANRLASTSLLEALLWGRNAALNIHGR
HHHHHCCHHCCCCCCHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEECCC
LGRRAALNKRLRDSIPDWNPLGDEKNDDPALIAQDWTTLRNTMWNYVGITRTSSRLQRAH
CCHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHEEECCHHHHHHHH
DEMRDLWRHVSDFYKRTPLSKPLVDLFHGCQAGYVVTMAARRNKQSVGCHYRID
HHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCCEEEEEEHHCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7737518; 10984043; 7961421 [H]