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The map label for this gene is rpoN [H]

Identifier: 78356814

GI number: 78356814

Start: 1825203

End: 1826627

Strand: Direct

Name: rpoN [H]

Synonym: Dde_1771

Alternate gene names: 78356814

Gene position: 1825203-1826627 (Clockwise)

Preceding gene: 78356813

Following gene: 78356815

Centisome position: 48.93

GC content: 50.67

Gene sequence:

>1425_bases
ATGGCTTTAGAGTTACGACAGCAGCTTAAACTCGCTCAGCAGCTTGTGATGACGCCCCAGCTTCAGCAGGCGATCAAACT
GTTGCAGCTTTCACGCGTCGAGCTCGTTGAAACCGTTCAGCAGGAACTGCTGGAAAACCCGTTTCTTGAAGAAACACAGG
AAGAGCGCCCAGTCTCTCAGGCAGAAGAGAGAGCCCCGAGAGCAGAAGAAGCCGACCCTTTCCGTCAGGAATTGGTAAAG
AATGCCGACTGGGAAAACTATCTTGGCGATTTTGCCAGTACCGGCAAACAGGCAGGCGGACGCGACGCGGAAGTGCCGGA
AGAGATGACCGCCTTTGAGGCTCGTTTTTCATCCAGCCCCACGCTGGAAGGGCATCTCAGCTGGCAGCTGCGCTTGTCTT
CCGCCACGGAAAAACAGCTGGACATAGCAGAGGTCATAATCGGCAACCTTTCGCCTGCGGGCTATCTGCAGGCGACGGTG
GAAAACGTGGCGGAAATGGCCGGTGCCGGTACGGCAGAGGTGGAAGAAACGCTGCACCTGCTGCAGCGGCTTGATCCCGT
GGGCATAGCGGCGCGTACTCCGCAGGAATGCCTTCTGGTTCAGGTCGAAGTGCTTGGATACGACCGCGACCCTGTGCTTG
TCGAGCTGATCCGCGATCATCTGGAAGATCTGGAAAAACGACGGTACAAGCCTCTTCTCAGAAAATATCGTCTGGGAATG
GACGACCTTAAAGAATATCTTGACCTTATCCAGACTCTTGACCCCATGCCGGGCTCCCGTTTCGGTAACGAACAGCCCGC
GTATGTCAGCCCCGATGTCTACGTGTATAAAAGCGAGGATGATTTTATCATCCTGCTGAACGAAGACGGCCTGCCACATC
TCCAGCTGAGTGAATTTTATAAAGAAGGCGTCAAGGGCACCACTGATAAAGAAAAGGACTACGTGCAGGAAAAAATGCGC
TCCGCCTCGTGGCTTATAAAAAGCCTTTATCAAAGGCAGCGAACCCTGTACAAAGTAGTTGAGAGCATTATTAAATATCA
GAGGGGTTTTTTCGAGGAAGGCGTATCCCGGCTTCGCCCGCTTATTCTCAAGGATATAGCGGACGACATAGGAATGCACG
AATCAACCGTCAGCCGTATTACAACAAACAAGTATGTGGCTACCCCCCATGGTATTGTGGAGCTTAAGTATTTCTTTAAC
AGTGCACTGGCGTTAAATGACGGAACAGAGGTAGGTTCTGAAAGTGTGAAAGCGCTGATAAAGAACCTGATTTCTGAAGA
AAATCCTAAAGAACCGCTCAGTGACGAAAAGATCGGAGAAATTCTTAAAGAAAAGTTGCAAGTCAATATTGCTCGAAGAA
CCGTGGCGAAGTATCGTACAGCTATGGGAATCCCTTCTTCTTCGAAACGAAAAGCTGTATTTTAG

Upstream 100 bases:

>100_bases
TATACGTTTCCGCTGTCTCCGGTATCAGGGCTTTGATATTCTCTGAGCAATAAAAACAAAATATATTCAGCTTTTATAAT
CTTAAGGATTCGGTTGAACA

Downstream 100 bases:

>100_bases
GCCAAAGGATTTCCGGCAGAAATGCCTGATACTGAATCTCTGGAGGTTTTTATGAACATCGCTTTCACTTTCAAGAACTT
CGAGCCTTCCGATCACTTGA

Product: sigma-54 factor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MALELRQQLKLAQQLVMTPQLQQAIKLLQLSRVELVETVQQELLENPFLEETQEERPVSQAEERAPRAEEADPFRQELVK
NADWENYLGDFASTGKQAGGRDAEVPEEMTAFEARFSSSPTLEGHLSWQLRLSSATEKQLDIAEVIIGNLSPAGYLQATV
ENVAEMAGAGTAEVEETLHLLQRLDPVGIAARTPQECLLVQVEVLGYDRDPVLVELIRDHLEDLEKRRYKPLLRKYRLGM
DDLKEYLDLIQTLDPMPGSRFGNEQPAYVSPDVYVYKSEDDFIILLNEDGLPHLQLSEFYKEGVKGTTDKEKDYVQEKMR
SASWLIKSLYQRQRTLYKVVESIIKYQRGFFEEGVSRLRPLILKDIADDIGMHESTVSRITTNKYVATPHGIVELKYFFN
SALALNDGTEVGSESVKALIKNLISEENPKEPLSDEKIGEILKEKLQVNIARRTVAKYRTAMGIPSSSKRKAVF

Sequences:

>Translated_474_residues
MALELRQQLKLAQQLVMTPQLQQAIKLLQLSRVELVETVQQELLENPFLEETQEERPVSQAEERAPRAEEADPFRQELVK
NADWENYLGDFASTGKQAGGRDAEVPEEMTAFEARFSSSPTLEGHLSWQLRLSSATEKQLDIAEVIIGNLSPAGYLQATV
ENVAEMAGAGTAEVEETLHLLQRLDPVGIAARTPQECLLVQVEVLGYDRDPVLVELIRDHLEDLEKRRYKPLLRKYRLGM
DDLKEYLDLIQTLDPMPGSRFGNEQPAYVSPDVYVYKSEDDFIILLNEDGLPHLQLSEFYKEGVKGTTDKEKDYVQEKMR
SASWLIKSLYQRQRTLYKVVESIIKYQRGFFEEGVSRLRPLILKDIADDIGMHESTVSRITTNKYVATPHGIVELKYFFN
SALALNDGTEVGSESVKALIKNLISEENPKEPLSDEKIGEILKEKLQVNIARRTVAKYRTAMGIPSSSKRKAVF
>Mature_473_residues
ALELRQQLKLAQQLVMTPQLQQAIKLLQLSRVELVETVQQELLENPFLEETQEERPVSQAEERAPRAEEADPFRQELVKN
ADWENYLGDFASTGKQAGGRDAEVPEEMTAFEARFSSSPTLEGHLSWQLRLSSATEKQLDIAEVIIGNLSPAGYLQATVE
NVAEMAGAGTAEVEETLHLLQRLDPVGIAARTPQECLLVQVEVLGYDRDPVLVELIRDHLEDLEKRRYKPLLRKYRLGMD
DLKEYLDLIQTLDPMPGSRFGNEQPAYVSPDVYVYKSEDDFIILLNEDGLPHLQLSEFYKEGVKGTTDKEKDYVQEKMRS
ASWLIKSLYQRQRTLYKVVESIIKYQRGFFEEGVSRLRPLILKDIADDIGMHESTVSRITTNKYVATPHGIVELKYFFNS
ALALNDGTEVGSESVKALIKNLISEENPKEPLSDEKIGEILKEKLQVNIARRTVAKYRTAMGIPSSSKRKAVF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of the nitrogen fixation genes. The open complex (sigma-54 and core

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=479, Percent_Identity=39.0396659707724, Blast_Score=327, Evalue=9e-91,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 53817; Mature: 53686

Theoretical pI: Translated: 4.74; Mature: 4.74

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALELRQQLKLAQQLVMTPQLQQAIKLLQLSRVELVETVQQELLENPFLEETQEERPVSQ
CCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
AEERAPRAEEADPFRQELVKNADWENYLGDFASTGKQAGGRDAEVPEEMTAFEARFSSSP
HHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
TLEGHLSWQLRLSSATEKQLDIAEVIIGNLSPAGYLQATVENVAEMAGAGTAEVEETLHL
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LQRLDPVGIAARTPQECLLVQVEVLGYDRDPVLVELIRDHLEDLEKRRYKPLLRKYRLGM
HHHCCCCCCCCCCCHHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DDLKEYLDLIQTLDPMPGSRFGNEQPAYVSPDVYVYKSEDDFIILLNEDGLPHLQLSEFY
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCEEEEECCCCEEEEECCCCCCCCHHHHHH
KEGVKGTTDKEKDYVQEKMRSASWLIKSLYQRQRTLYKVVESIIKYQRGFFEEGVSRLRP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILKDIADDIGMHESTVSRITTNKYVATPHGIVELKYFFNSALALNDGTEVGSESVKALI
HHHHHHHHHHCCCHHHHHHHHCCCEEECCCCHHHHHHHHCCHHEECCCCCCCHHHHHHHH
KNLISEENPKEPLSDEKIGEILKEKLQVNIARRTVAKYRTAMGIPSSSKRKAVF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
ALELRQQLKLAQQLVMTPQLQQAIKLLQLSRVELVETVQQELLENPFLEETQEERPVSQ
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHH
AEERAPRAEEADPFRQELVKNADWENYLGDFASTGKQAGGRDAEVPEEMTAFEARFSSSP
HHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCC
TLEGHLSWQLRLSSATEKQLDIAEVIIGNLSPAGYLQATVENVAEMAGAGTAEVEETLHL
CCCCEEEEEEEECCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LQRLDPVGIAARTPQECLLVQVEVLGYDRDPVLVELIRDHLEDLEKRRYKPLLRKYRLGM
HHHCCCCCCCCCCCHHHHHEEHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCH
DDLKEYLDLIQTLDPMPGSRFGNEQPAYVSPDVYVYKSEDDFIILLNEDGLPHLQLSEFY
HHHHHHHHHHHHCCCCCCCCCCCCCCCEECCCEEEEECCCCEEEEECCCCCCCCHHHHHH
KEGVKGTTDKEKDYVQEKMRSASWLIKSLYQRQRTLYKVVESIIKYQRGFFEEGVSRLRP
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LILKDIADDIGMHESTVSRITTNKYVATPHGIVELKYFFNSALALNDGTEVGSESVKALI
HHHHHHHHHHCCCHHHHHHHHCCCEEECCCCHHHHHHHHCCHHEECCCCCCCHHHHHHHH
KNLISEENPKEPLSDEKIGEILKEKLQVNIARRTVAKYRTAMGIPSSSKRKAVF
HHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2999700; 2695747 [H]