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The map label for this gene is hprA [H]

Identifier: 78356724

GI number: 78356724

Start: 1731810

End: 1732778

Strand: Reverse

Name: hprA [H]

Synonym: Dde_1681

Alternate gene names: 78356724

Gene position: 1732778-1731810 (Counterclockwise)

Preceding gene: 78356731

Following gene: 78356722

Centisome position: 46.45

GC content: 57.48

Gene sequence:

>969_bases
ATGCGCATCACTGTGCTTGACGGATACACCCTGAACCCGGGAGACCTTTCCTGGGCAGAAATCGAAAAACTGGGAGAAGT
TACTGTTCATGACAGAACGCATGGACAGGATATTGTGAAACGGGGCGGGCAGGCGCAGATACTTTTGACCAACAAAGTCC
GTCTGACCGCCGAAACGCTGGCGATGCTGCCGCAGCTCCGGTTCATATCGGTTCTTGCCACCGGATATGACGTGGTGGAC
ATAGCCGCAGCCGCAGCCAGAGGCATACCGGTATCAAATGCACCGGGATACGGGGTGGAAGCCGTCGCCCAGCATACCAT
GGCACTGTTGCTTGAATTATGCAGAAAAACAGCGCGCCACGATACGCTTGTCAAACAGGGAGCATGGTCTCAGGCCCCTG
ACTGGTGCTTCTGGGAAGGAACGCAGCAGCAGCTTACCGGAAAAACCATGGGGATTGTCGGGTTCGGCAATTCAGGACGT
AGGGTTGCGGTACTGGCCGACGCTTTCGGCATGGATGTCATAGCCTATGCTCCGCGCCCCAAGGAAGCTCCGGCACTCAG
AAATTTCAGATTTGCCCCGCTGGAAGAACTGACGGCACAGGCGGACGTCATCAGCCTGCACTGCCCTCTCACCGCCGACA
ACCGTCATCTGATAAACGCGCAGCGCATAGCGTCGATGAAAGACGGCGCGCTGCTGCTGAACACGGCACGGGGACCGCTG
GTGGACGAAACGGCTCTGGCACAGGCACTGGTGTCGGGCAAACTGGGCGGAGCCGGTCTGGATGTGCTGGAGACGGAACC
TCCGCTTCCGGACAATCCGCTTTTCCGTGCCCCCAATTGCCTTATCACCCCTCATATTGCATGGGCTACACAGACTGCCC
GGCAATCACTGATGAGCATAACAGCCCGCAACATCGAGATGTTCAAACACGGCACACCGCAAAATGTGGTCAACGCGCAC
ATGCTGTGA

Upstream 100 bases:

>100_bases
CAACTCTTTCAAATTGACACCGCAGGCGGAATAAGGTCCCATCCTGCTTTCCGGTATCATCGCCAAGGCATTCAGTACCA
CTACCTACAAGGAGAAAGCC

Downstream 100 bases:

>100_bases
TCAGAAAGAAAGGCGGGGTGCCGCAGCACCCCGCCTTCTAGTGTCGTTACGGTTTGAAAGGGCTAAATTTTGCCCATGGG
GTCTTCGCGCAGCATTTTCA

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRITVLDGYTLNPGDLSWAEIEKLGEVTVHDRTHGQDIVKRGGQAQILLTNKVRLTAETLAMLPQLRFISVLATGYDVVD
IAAAAARGIPVSNAPGYGVEAVAQHTMALLLELCRKTARHDTLVKQGAWSQAPDWCFWEGTQQQLTGKTMGIVGFGNSGR
RVAVLADAFGMDVIAYAPRPKEAPALRNFRFAPLEELTAQADVISLHCPLTADNRHLINAQRIASMKDGALLLNTARGPL
VDETALAQALVSGKLGGAGLDVLETEPPLPDNPLFRAPNCLITPHIAWATQTARQSLMSITARNIEMFKHGTPQNVVNAH
ML

Sequences:

>Translated_322_residues
MRITVLDGYTLNPGDLSWAEIEKLGEVTVHDRTHGQDIVKRGGQAQILLTNKVRLTAETLAMLPQLRFISVLATGYDVVD
IAAAAARGIPVSNAPGYGVEAVAQHTMALLLELCRKTARHDTLVKQGAWSQAPDWCFWEGTQQQLTGKTMGIVGFGNSGR
RVAVLADAFGMDVIAYAPRPKEAPALRNFRFAPLEELTAQADVISLHCPLTADNRHLINAQRIASMKDGALLLNTARGPL
VDETALAQALVSGKLGGAGLDVLETEPPLPDNPLFRAPNCLITPHIAWATQTARQSLMSITARNIEMFKHGTPQNVVNAH
ML
>Mature_322_residues
MRITVLDGYTLNPGDLSWAEIEKLGEVTVHDRTHGQDIVKRGGQAQILLTNKVRLTAETLAMLPQLRFISVLATGYDVVD
IAAAAARGIPVSNAPGYGVEAVAQHTMALLLELCRKTARHDTLVKQGAWSQAPDWCFWEGTQQQLTGKTMGIVGFGNSGR
RVAVLADAFGMDVIAYAPRPKEAPALRNFRFAPLEELTAQADVISLHCPLTADNRHLINAQRIASMKDGALLLNTARGPL
VDETALAQALVSGKLGGAGLDVLETEPPLPDNPLFRAPNCLITPHIAWATQTARQSLMSITARNIEMFKHGTPQNVVNAH
ML

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=262, Percent_Identity=39.6946564885496, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI4557499, Length=262, Percent_Identity=39.6946564885496, Blast_Score=172, Evalue=5e-43,
Organism=Homo sapiens, GI61743967, Length=262, Percent_Identity=38.1679389312977, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI4557497, Length=262, Percent_Identity=38.1679389312977, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI145580575, Length=262, Percent_Identity=39.6946564885496, Blast_Score=166, Evalue=3e-41,
Organism=Homo sapiens, GI23308577, Length=278, Percent_Identity=33.8129496402878, Blast_Score=140, Evalue=1e-33,
Organism=Homo sapiens, GI6912396, Length=253, Percent_Identity=35.1778656126482, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI87082289, Length=284, Percent_Identity=36.9718309859155, Blast_Score=159, Evalue=2e-40,
Organism=Escherichia coli, GI1787645, Length=261, Percent_Identity=33.3333333333333, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI1789279, Length=278, Percent_Identity=33.4532374100719, Blast_Score=139, Evalue=3e-34,
Organism=Escherichia coli, GI87081824, Length=166, Percent_Identity=36.7469879518072, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788660, Length=254, Percent_Identity=31.496062992126, Blast_Score=75, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=302, Percent_Identity=33.7748344370861, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI25147481, Length=278, Percent_Identity=33.0935251798561, Blast_Score=141, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6320925, Length=263, Percent_Identity=32.6996197718631, Blast_Score=139, Evalue=5e-34,
Organism=Saccharomyces cerevisiae, GI6322116, Length=263, Percent_Identity=32.319391634981, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6324055, Length=313, Percent_Identity=30.6709265175719, Blast_Score=132, Evalue=8e-32,
Organism=Saccharomyces cerevisiae, GI6324964, Length=235, Percent_Identity=34.0425531914894, Blast_Score=114, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6324980, Length=152, Percent_Identity=36.8421052631579, Blast_Score=89, Evalue=8e-19,
Organism=Saccharomyces cerevisiae, GI6325144, Length=168, Percent_Identity=30.952380952381, Blast_Score=71, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321253, Length=155, Percent_Identity=33.5483870967742, Blast_Score=70, Evalue=3e-13,
Organism=Drosophila melanogaster, GI24646446, Length=263, Percent_Identity=39.9239543726236, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24646448, Length=263, Percent_Identity=39.9239543726236, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24646452, Length=263, Percent_Identity=39.9239543726236, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI24646450, Length=263, Percent_Identity=39.9239543726236, Blast_Score=166, Evalue=2e-41,
Organism=Drosophila melanogaster, GI62472511, Length=263, Percent_Identity=39.9239543726236, Blast_Score=164, Evalue=7e-41,
Organism=Drosophila melanogaster, GI28571528, Length=262, Percent_Identity=37.7862595419847, Blast_Score=138, Evalue=6e-33,
Organism=Drosophila melanogaster, GI19921140, Length=251, Percent_Identity=33.8645418326693, Blast_Score=128, Evalue=5e-30,
Organism=Drosophila melanogaster, GI24585514, Length=256, Percent_Identity=31.640625, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574282, Length=256, Percent_Identity=31.640625, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=31.640625, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=31.640625, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=31.640625, Blast_Score=125, Evalue=3e-29,
Organism=Drosophila melanogaster, GI28574286, Length=263, Percent_Identity=30.7984790874525, Blast_Score=124, Evalue=7e-29,
Organism=Drosophila melanogaster, GI24585516, Length=251, Percent_Identity=30.2788844621514, Blast_Score=117, Evalue=8e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 34788; Mature: 34788

Theoretical pI: Translated: 7.29; Mature: 7.29

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRITVLDGYTLNPGDLSWAEIEKLGEVTVHDRTHGQDIVKRGGQAQILLTNKVRLTAETL
CEEEEECCEEECCCCCCHHHHHHHCCEEECCCCCCHHHHHCCCCEEEEEECCEEHHHHHH
AMLPQLRFISVLATGYDVVDIAAAAARGIPVSNAPGYGVEAVAQHTMALLLELCRKTARH
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DTLVKQGAWSQAPDWCFWEGTQQQLTGKTMGIVGFGNSGRRVAVLADAFGMDVIAYAPRP
HHHHHCCCCCCCCCCEEECCCHHHHCCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCC
KEAPALRNFRFAPLEELTAQADVISLHCPLTADNRHLINAQRIASMKDGALLLNTARGPL
CCCCHHHCCCCCCHHHHHCCCCEEEEECCCCCCCCEEECHHHHHCCCCCEEEEECCCCCC
VDETALAQALVSGKLGGAGLDVLETEPPLPDNPLFRAPNCLITPHIAWATQTARQSLMSI
CCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
TARNIEMFKHGTPQNVVNAHML
HHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure
MRITVLDGYTLNPGDLSWAEIEKLGEVTVHDRTHGQDIVKRGGQAQILLTNKVRLTAETL
CEEEEECCEEECCCCCCHHHHHHHCCEEECCCCCCHHHHHCCCCEEEEEECCEEHHHHHH
AMLPQLRFISVLATGYDVVDIAAAAARGIPVSNAPGYGVEAVAQHTMALLLELCRKTARH
HHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
DTLVKQGAWSQAPDWCFWEGTQQQLTGKTMGIVGFGNSGRRVAVLADAFGMDVIAYAPRP
HHHHHCCCCCCCCCCEEECCCHHHHCCCEEEEEEECCCCCEEEEEECCCCCEEEEECCCC
KEAPALRNFRFAPLEELTAQADVISLHCPLTADNRHLINAQRIASMKDGALLLNTARGPL
CCCCHHHCCCCCCHHHHHCCCCEEEEECCCCCCCCEEECHHHHHCCCCCEEEEECCCCCC
VDETALAQALVSGKLGGAGLDVLETEPPLPDNPLFRAPNCLITPHIAWATQTARQSLMSI
CCHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHH
TARNIEMFKHGTPQNVVNAHML
HHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]